Date post: | 13-Jan-2016 |
Category: |
Documents |
Upload: | walter-harrington |
View: | 218 times |
Download: | 1 times |
Logistical Considerations for Participation in the PSI-MR
John G. Primm, Craig A. Bingman, Gary Wesenberg, Xiaokang Pan, Russell L. Wrobel, Kory D. Seder, Brian G. Fox, George N. Phillips, Jr.,
and John L. Markley
• Scope of CESG individual activity
• Current administrative process for CESG material distribution / Stakeholders
• New infrastructure developed for participation in PSI-MR
• Cost / Benefit analysis
• Considerations and Going Forward
PSI-2 Material Transfer Agreement Activity
0
5
10
15
20
25
30
35
Material Transfer Agreements Initiated
Year 1
Year 2
Year 3
Our most popular request…
CESG Materials Distribution PSI-2
Current Processes
Stakeholders
Step Action Cost Units Involved
1 Incoming request for material sent from Requestor to PI or comes in through CESG web site. $26.89 PI
2 Request is routed to PM office - preliminary review. $15.30 PI / PM
3 If the material can be shared, material location is extracted from LIMS and verified. $13.24 Cloning / PM
4Project Management office (PM) sets up files and contacts requestor to establishing legal signing entity for their institution. $4.64
PM office / Requestor
5Upon receipt of Legal information, a boiler plate MTA is form is filled out and UW routing forms are constructed; routing for execution begins. $21.02
PM assistant / PM / PI
6 The PI reviews and signs the documents. $15.30 PM / PI
7 Documents routed to UW Biochemistry chair for review / signature. Copies made and filed. $15.30 PM / Biochem
8Documents routed to UW College of Agriculture and Life Sciences (CALS) for review / signature. Entered into CALS database. Copies filed. $20.09
PM assistant / CALS intake /CALS
9Wisconsin Alumni Research Foundation (WARF) may be brought in depending on materials / issues. $3.09 CALS / WARF
10
After CALS / WARF review, documents are routed to UW Research and Sponsored Programs Office (RSP). MTA entered into RSP database. MTA reviewed and signed. Copies made and filed. $13.91 RSP intake / RSP
11 RSP routes to legal signatory for institutional signature. $28.91 RSP intake / RSP
12 Legal signatory routes to recipient scientist for external Requestor signature. $0.00Tech Transfer Office / Requestor
13 Executed copy returned to RSP, originals filed, RSP database status marked as "completed“. $10.43Tech Transfer Office / RSP
14 PM office notifies cloning; cloning pulls and prepares material. $54.94 Cloning / PM
15 Materials pass through QA step. $84.41 Cloning
16 Material is packed and sent to Requestor. $32.65 Cloning / PM
17 Specific information regarding the sample is sent to the Requestor. $13.24 Cloning
18Follow-up questions from the Requestor are directed to and answered by the appropriate CESG group. $26.47
Requestor / Cloning / PM
Total Cost for Average MTA and Transfer $399.80
Stakeholder IssuesIntellectual Property > UW licenses thousands of
products now in commerceLiabilityTracking of materials depositedTracking of materials distributedTracking of materials receivedOrganization and visibility of processesPermanent storage of recordsDistribution efforts
Physical / extraction of manipulation of data
Image from photobucket.com
Nuts
and
Bolts
Data First !
Plates last!
Clone Information Files (Required):
• Clone Location File
• Clone Information File
• Clone Gene Info File
Sesame actions required for internal tracking
Sesame search results
Human Eyes 2 hrs/WG
Permanent data storage
PSI-MR CESG Quarterly Report Order ID Order Date Status Clone ID987 FEB-06-2008 10:37:40 Shipped AtCD00082681983 FEB-04-2008 16:36:05 Shipped AtCD00082683
Species Specific ID User At1g70910 David AcetiAt4g16160 David Aceti
Shipping Information: CESG-QA 445 Henry Mall Madison, WI 53706 Phone: 608-890-0491User Email: [email protected] Name MARKLEY, John PI Institution Univ. Wisconsin-Madison PI Department Biochemistry PI Email [email protected]
Results to date
Viability of Destination Clones by Workgroup
0
20
40
60
80
100
WG
834
WG
875
WG
917
WG
925
WG
935
WG
975
WG
102
5
WG
119
1
WG
119
2
WG
119
7
WG
120
8
WG
123
0
WG
125
5
WG
126
5
WG
130
6
WG
132
2
WG
132
3
WG
138
5
Submitted
Transformed
% Transformed
CESG PSI-MR Deposition Progress as of 4/10/08
0200400600800
1000120014001600
TransformationAttempted
Transformed Accepted Rejected /mismatch
In Progress
De
sti
na
tio
n C
lon
es
Accepted Destination Clones By Workgroup
0
20
40
60
80
100
WG 8
34
WG 8
75
WG 9
17
WG925
WG 9
35
WG 9
75
WG 1
025
WG 1
191
WG 1
192
WG 1
197
WG 1
208
WG 1
230
WG 1
255
WG 1
265
WG 1
306
WG 1
322
WG 1
323
WG 1
385
% Transformed
% Accepted
Accepted Destination Clones By Workgroup
0
20
40
60
80
100
WG 8
34
WG 8
75
WG 9
17
WG925
WG 9
35
WG 9
75
WG 1
025
WG 1
191
WG 1
192
WG 1
197
WG 1
208
WG 1
230
WG 1
255
WG 1
265
WG 1
306
WG 1
322
WG 1
323
WG 1
385
% Transformed
% Accepted
Of the sample:
CESG was able to positively identify 83 % of those mismatched.
Pathway for remediation is under discussion.
Next GoalsReduce mismatched error rate 13.7% > by
remediation to 7.0%
Deposit all “declared available” destination clones by Oct 1. Or, better, before any changes in the data format occurs.
Improve CESG processes to include multiple vectors clones
Unit Estimated time HRS / 500 destination clones
Estimated cost / 500 destination clones
Projected costs - all remaining destination clones
Projected costs - all remaining entry clones
Bioinformatics 14 $711 $7,113 $1,423
Cloning 8 $424 $4,235 $5,929
QA 8 $349 $3,486 $697
CESG Admin 0.5 $31 $306 $428
RSP 0.1 $4 $42 $58
CALS 0.1 $5 $48 $67
Totals MR deposition 30.7 $1,523 $15,231 $8,604
Totals for traditional distribution $45,885
Projected Savings $22,051
Costs of ImplementationUnit Estimated time HRS Investment
Bioinformatics 280 $15,105.35
Cloning 80 $4,235.28
QA 20 $871.53
CESG Admin 45 $4,158.33
RSP 10 $417.22
CALS 18 $867.83
Cost of MR Participation Summary
Infrastructure Investment $24,700
Projected Savings ($22,000)
Priceless!
Looking Forward•PSI-MR will receive significant attention as useful biological research materials for biomedically relevant proteins whose purification properties and structures are known become available.
•Each Center preparing TargetDB files should already have accumulated all or nearly all of the information needed to prepare clone submission files for the PSI-MR.
•Opportunities are now emerging to create a wonderful synergy between the PSI-MR, PepcDB, and the PSI Knowledgebase.
– require that a unified, searchable tag is seeded into all three databases
Opportunity
Risk Requestors need and expect
all information required
(Protocols) to replicate your
success.
Data Preparation Depositors Agreement
Data Preparation Depositors Agreement
Data Preparation Depositors Agreement
Expedited MTA Covers Entire Institutions
Data Preparation Depositors Agreement
Expedited MTA Covers Entire Institutions
Data Preparation Depositors Agreement
Expedited MTA Covers Entire Institutions
Data Preparation Depositors Agreement
Expedited MTA Covers Entire Institutions
Data Preparation Depositors Agreement
Dancing in the
Streets
Acknowledgements
CESG gratefully acknowledges the collaborative interactions with the following personnel of the PSI-MR as an essential part of this effort: Catherine Cormier, Joshua LaBaer, Stephanie Mohr, Andreas Rolfs, Elena Taycher, and Dongmei Zuo.
We also thank all members of CESG, including Arash Bahrami, Lenka Bittova, Lai Bergeman, E. Sethe Bergie, Christopher Bianchetti, Eduard Bitto, Claudia Cornilescu, Ronnie Frederick, Michael Goren, Katarzyna Gromek, Leigh Grundhoefer, Teagan Hayes, Andrew Larkin, Elena Levin, Betsy Lytle, Shin-Ichi Makino, John Markley, Yuko Matsubara, Karl Nichols, Xiaokang Pan, Francis Peterson, Mike Popelars, Sarata Sahu, Kory Seder, Donna Troestler, Frank Vojtik, Brian Volkman, Gary Wesenberg, Russell Wrobel, and Zsolt Zolnai for their support of this important initiative.