Long-term Potentiation as a Physiological Phenomenon
From Mechanisms of Memory by J. David Sweatt, Ph.D.
The Cellular and Molecular Basis of Cognition
Memories are stored as alterations in the strength of synaptic connections between neurons in the CNS.
“Hebb’s Postulate”:
When an axon of cell A … excites cell B and repeatedly or persistently takes part in firing it, some growth process or metabolic change takes place in one or both cells so that A’s efficiency as one of the cells firing B is increased.
D.O. Hebb, The Organization of Behavior, 1949.
From Sidney Harris
Memories are stored as alterations in the strength of synaptic connections between neurons in the CNS.
“Hebb’s Postulate”:
When an axon of cell A … excites cell B and repeatedly or persistently takes part in firing it, some growth process or metabolic change takes place in one or both cells so that A’s efficiency as one of the cells firing B is increased.
D.O. Hebb, The Organization of Behavior, 1949.
TVP Bliss, FRS
EntorhinalCortex
Dentate Gyrus
CA3
IpsilateralCA1
PerforantPathway
Mossy Fiber
SchafferCollaterals
The Entorhinal/Hippocampal System
Str
atu
m L
acu
no
som
Mo
lecu
lar
inp
uts
RecurrentConnections
Bliss and Lomo’s First Published LTP Experiment
Lateral Septum,
Contralateral CA1
EntorhinalCortex
Dentate Gyrus
CA3
IpsilateralCA1
PerforantPathway
Mossy Fiber
EntorhinalCortex
Subiculum Lateral Septum
Amygdala,Cortex
SchafferCollaterals
Norepinephrine,
Acetylcholine,
Serotonin
GABAergic Interneuron
CA1 Axon
Schaffer Collaterals
The Entorhinal/Hippocampal System
Str
atu
m L
acu
no
som
Mo
lecu
lar
inp
uts
RecurrentConnections
SLM Inputs
Dopamine,
The Dendritic Tree
The Dendritic Spine
Lateral Septum,
Contralateral CA1
EntorhinalCortex
Dentate Gyrus
CA3
IpsilateralCA1
PerforantPathway
Mossy Fiber
EntorhinalCortex
Subiculum Lateral Septum
Amygdala,Cortex
SchafferCollaterals
Norepinephrine,
Acetylcholine,
Serotonin
GABAergic Interneuron
CA1 Axon
Schaffer Collaterals
The Entorhinal/Hippocampal System
Str
atu
m L
acu
no
som
Mo
lecu
lar
inp
uts
RecurrentConnections
SLM Inputs
Dopamine,
StimulatingElectrode
RecordingElectrode
Electrodes in a Living Hippocampal Slice
Tissue Slice Chamber
Stimulating Schaffer Collaterals
in Area CA3
Recording in Stratum Pyramidale
in Area CA1
Recording in Stratum Radiatum
in Area CA1
Stimulus Artifact
Fiber VolleyEPSP
Recording Configuration and Typical Responses in a Hippocampal Slice Recording Experiment
-20 0 20 40 60 80 1000
1
2
3
Time (min)
No
rmal
ized
Initi
al S
lope
-20 0 20 40 60 80 1000
1
2
3
Time (min)
No
rmal
ized
Initi
al S
lopeB
A Input/Output
0 10 20 30 400
250
500
750
Stimulus Intensity ( A)
Slo
pe
fEP
SP
( V
/ms)
Fiber Volley
0 10 20 30 400.0
0.1
0.2
0.3
0.4
Stimulus Intensity ( A)
Fib
er V
olle
y A
mp
litu
de
(V
)
Input/Output vs Fiber Volley
0.0 0.1 0.2 0.3 0.40.00
0.25
0.50
0.75
Fiber Volley Amplitude ( V)
Slo
pe
fEP
SP
( V
/ms)
An Input/Output Curve and a Typical LTP Experiment
From Nicoll et al.
Malenka et al, Bear et al, Huganir et al.
Theta Pattern in Hippocampal EEG
1-voluntary movement 2-REM sleep3-still-alert 4-slow-wave sleep
Before and after a
medial septal lesion.
…
100-Hz 100-Hz 100-Hz 100-Hz
200
msec200
msec
200
msec
10 msec between pulses
• 5-Hz burst frequency• 10 bursts per train• 3 trains, 20-sec intertrain interval
A
B
Time(min)
fEP
SP
slo
pe
(% o
f b
asel
ine)
-20 0 20 40 60
75
100
125
150
175
200
LTP Triggered by Theta Burst Stimulation
Pairing LTP
ASSOCIATIVE LTP
German Barrionuevo and Tom Brown
Back Propagating Action Potentials
Pairing LTP
NEURONAL INFORMATION PROCESSING
MOLECULAR MECHANISMS
NMDA
APV = AP5
Graham Collingridge
-20 0 20 40 60
75
100
125
150
175
200
225Vehicle50 M APV
APV
Time(min)
fEP
SP
slo
pe
(% o
f b
asel
ine)
APV Block of LTP
Gly
Glu++++
----
---
+++
SynapticGlutamate Alone
CytoplasmSynaptic Cleft
Mg++
Ca++
Glu
Ca++
++++
----
Mg++
Gly
---
+++
Glutamate plusMembrane
Depolarization
CytoplasmSynaptic Cleft
Ca++
Coincidence Detection by the NMDA Receptor
Back Propagating Action Potentials
Timing of Back-propagating Action Potentials with Synaptic Activity
EP
SP
’s
SynapticActivity
NEChange in
Local excitability1
2
Synapse
LTP?
A B
The Dendritic Tree and Regulation of Action Potential Propagation
NMDAR Independent LTP
200 Hz
Mossy FiberTEA LTP
-30 -20 -10 0 10 20 30 40 50 6050
75
100
125
150
175
200
200HzAP-5
Time (min)%
Slo
pe
pE
PS
P(S
tan
dar
diz
ed t
o B
asel
ine)
-90 -60 -30 0 30 60 90 120 150 18080
100
120
140
160
180
200
Seconds%
bas
elin
e fE
PS
P
PTPPPF