LSBRLSBR

Single particle processing workflow in

BsoftBernard Heymann

SPA Processing Steps

Picking

Corrections/Modifications (CTF, mask)

Alignment

Reconstruction

Quality assessment (Resolution)

Bsoft SPA Workflow

Parameter Organization

Parameter Handling

Flat files:

Text files with tagged parameters

Images referenced through tags

Multiple formats: STAR & XML

Backwards compatible

Types of Bsoft parameter files:1.Micrograph/reconstruction parameter files2.Modeling parameter files

Quality Assessment

Reconstruction:Resolution/spatial frequency limit.

Modification curve (sharpening, B-factor)

Number of particles in reconstruction

Defocus range, astigmatism range

View distribution

Coverage/Completeness (up to spatial frequency limit)

Particle FOM distribution

Resolution: FSC curve or similar

ModelingFitting statistics

Reconstruction assessment

View distribution ResolutionFOM distribution