M. Willrich Siebert, L. B. Braxton, R. M. Huckaba, L.C. Walton, R.A. Haygood,

Field Performance of WideStrike Field Performance of WideStrike Insect Insect ProtectionProtection Against Key Lepidopteran Pests in Against Key Lepidopteran Pests in the Mid-South And Southeastern U.S.the Mid-South And Southeastern U.S.

M. Willrich Siebert, L. B. Braxton, R. M. Huckaba, L.C. Walton, R.A. Haygood, R.B. Lassiter, F. Haile, and G. D. Thompson

*Dow AgroSciences LLC., Indianapolis, IN

TM Trademark of Dow AgroSciences LLC.TM PhytoGen and the PhytoGen Logo are trademarks of PhytoGen Seed Company LLC.

TMTM

Heliothine Complex In CottonHeliothine Complex In Cotton

• Bollworm, Helicoverpa zea

• Tobacco Budworm, Heliothis virescens

• Mid-south and southeastern U.S.

• 2005 Cotton Insect Loss Estimates:

- Yield Reduction: 1.2% (10 states, 1 to 2.5%) - Applications/Acre: 0.8 (0.2 to 1.5)


Secondary Lepidopteran Pests In Cotton Secondary Lepidopteran Pests In Cotton

2005• Applications per acre: <1

• Fall armyworm: Greatest losses among secondary Lepidoptera

Fall Armyworm, Beet Armyworm, Southern Armyworm Soybean Looper/Cabbage Looper


Cry 1Ac

Cry 1F

+

WideStrike : A Dual-Gene WideStrike : A Dual-Gene BtBt Technology For Broad Technology For Broad Spectrum Lepidopteran ManagementSpectrum Lepidopteran Management

Commercially Available In PhytoGen Varieties Since 2005

TMTM


• WideStrikeTM reduced feeding damage by tobacco budworm and bollworm to low levels.

Cry1Ac is highly effective against tobacco budworm.WideStrikeTM sustained low-level damage under high bollworm pressure

• WideStrikeTM non-sprayed yields were numerically similar if not statistically greater than the sprayed non-Bt in natural infestation tests.

• WideStrikeTM provided excellent control of many of the secondary foliar feeding lepidopterous insects including cabbage looper, soybean looper, southern armyworm, and fall armyworm.

Historical Background: 2001-2005Historical Background: 2001-2005


Materials And MethodsMaterials And Methods WideStrike Evaluated in 23 Locations During 2006 - No

Insecticide Sprays Targeting Lepidopteran Pests - Natural Infestations

Experimental Design: Randomized Complete Block, 4 replications

Variables Measured: Heliothine Damage And Live Larvae, and Lint Yield

Statistical Analysis: ANOVA, α = 0.10

TM


WideStrike Trial Locations: 2006 WideStrike Trial Locations: 2006

* High* Moderate

* Low

Heliothine Infestations

*

*

*

*** *

*

* ****

**

* **

** *

* *

TMTM


0

20

40

60

80

100

Perc

ent

8 Aug

PHY370WR PHY485WRF PHY310R PHY425RF

8 Aug 15 Aug 24 Aug

Square Damage Boll Damage

Efficacy of WideStrike Against Bollworm,Efficacy of WideStrike Against Bollworm,Rocky Mount, NC, Dr. J.R. Bradley, NCSURocky Mount, NC, Dr. J.R. Bradley, NCSU

Columns within a date followed by the same letter are not significantly different, P>0.10

b b b b b b bb

aa

aa

a

aa

a

TMTM


Performance of WideStrike Against Bollworm,Performance of WideStrike Against Bollworm,East Bernard, TX, Gregory Visoski Consulting EntomologistEast Bernard, TX, Gregory Visoski Consulting Entomologist

0

20

40

60

80

100

Perc

ent

2 July


2 July 12 July 7 Aug

Square Damage Boll DamageColumns within a date followed by the same letter are not significantly different, P>0.10

PHY470WR PHY480WR

aa

aa

aaa

a

b b

b

b

TMTM


0

200

400

600

800

1000

Lint Yield / Acre

Poun

ds

b

b

aba

b

c c

Yield of WideStrike Varieties Exposed to High Bollworm Pressure,Yield of WideStrike Varieties Exposed to High Bollworm Pressure, East Bernard, TX, Gregory Visoski Consulting Entomologist East Bernard, TX, Gregory Visoski Consulting Entomologist


PHY370WR PHY485WRF PHY310R PHY425RFPHY470WR PHY480WR

TMTM


Performance of WideStrike Against Bollworm,Performance of WideStrike Against Bollworm,Bossier City, LA, Steve Micinski, LSU AgCenterBossier City, LA, Steve Micinski, LSU AgCenter



0

10

20

30

40

50

Squa

re D

amag

e (%

)

11 July 18 July00 00

a

a

a

a

a

25 July 1 Aug

a

a

a

00 00 0000

b b b b

TMTM


0

300

600

900

1200

Lint Yield / Acre

Poun

ds

a

b

a aa

b b



Yields of WideStrike Varieties Exposed ToYields of WideStrike Varieties Exposed ToSustained Bollworm Infestations, Bossier City, LASustained Bollworm Infestations, Bossier City, LA

Steve Micinski, LSU AgCenterSteve Micinski, LSU AgCenter

TMTM


Square Damage (%) Boll Damage (%)

26 Jul 2 Aug 9 Aug 23 Aug 30 Aug 26 Jul 2 Aug 9 Aug 23 Aug 30 Aug

*Fall armyworm infestations occurred from 9-30 Aug.


Efficacy of WideStrike Against Mixed Bollworm andEfficacy of WideStrike Against Mixed Bollworm andFall Armyworm Infestations, Lonoke, ARFall Armyworm Infestations, Lonoke, ARDr. Merritt Holman, Arkansas Crop TechnologyDr. Merritt Holman, Arkansas Crop Technology

TMTM


0

3

6

9

12

Cum

ulat

ive

Num

ber L

arva

e

9 Aug*Number per 40 bolls sampled per date.

23 Aug 30 Aug


WideStrike Provides Excellent Control Of Fall Armyworm, Lonoke, ARWideStrike Provides Excellent Control Of Fall Armyworm, Lonoke, ARDr. Merritt Holman, Arkansas Crop TechnologyDr. Merritt Holman, Arkansas Crop Technology

TMTM


0

300

600

900

1200

1500

Lint Yield / Acre

Poun

ds

b

a a

b

c c


Yields of WideStrike Varieties Exposed To Sustained Yields of WideStrike Varieties Exposed To Sustained Bollworm and Fall Armyworm Infestations, Lonoke, ARBollworm and Fall Armyworm Infestations, Lonoke, AR


Dr. Merritt Holman, Arkansas Crop TechnologyDr. Merritt Holman, Arkansas Crop Technology

TMTM


0

200

400

600

800

1000

1200

No. larvae /100 row-ft

PHY 310 RPHY 425 RFPHY 370 WRPHY 485 WRF

Efficacy of WideStrike Against High-Level Soybean Looper Efficacy of WideStrike Against High-Level Soybean Looper Infestations, Tillar, ARInfestations, Tillar, AR

Dr. Charlie Guy, G&H Associates, Inc.Dr. Charlie Guy, G&H Associates, Inc.

a

a

b b


TMTM


ConclusionsConclusions Efficacy of WideStrike™ cotton against Heliothines in 2006 similar

to 2001-2005 results.

Varieties containing WideStrike provided control of Heliothis (primarily bollworm) that were statistically similar.

- Boll damage exceeded 5% at four of 23 locations, all under heavy BW pressure.

- Under high insect occurrence, a few locations exceeded insect threshold levels of feeding damage or larval infestation in Widestrike™ cotton – treatment necessary to prevent losses from bollworm.


ConclusionsConclusions Varieties containing WideStrike provided control of secondary

lepidopteran pests that were statistically similar. WideStrike effectively protected against yield loss.

– Numerical and even statistic differences did occur among WideStrike varieties in one location. Yield potential and environmental conditions are likely contributing factors, rather than in-season differences in efficacy.

TM


WideStrike Insect Protection:WideStrike Insect Protection:Delivery of Two Insect Control GenesDelivery of Two Insect Control Genes

Excellent Efficacy Against Heliothines

Control Of Key Secondary Lepidopteran Pests

Overall Reduction In Lepidopteran Sprays

Expressed In High Yielding PhytoGen Varieties

TMTM


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