Methylation inhibit gene expression

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1

-1

-0.5

0

0.5

1

1.5

2

2.5

3

3.5

4

f(x) = − 0.456651984224308 x + 0.28621292191235R² = 0.048288207228155

MAGEB2

Methylation

Gene

exp

ress

ion

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.4

0.5

f(x) = − 0.00114300925953714 x + 0.0058958753028404R² = 1.66320590794244E-05

MAGEA2B

Methylation

Gene

exp

ress

ion

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.4

0.5

f(x) = − 0.001943022608513 x + 0.00606922636890706R² = 3.1715406761279E-05

MAGEA2B

Methylation

Gene

exp

ress

ion

(2nd

)

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4

-1.5

-1

-0.5

0

0.5

1

1.5

f(x) = 0.197843446856895 x + 0.0137069192056319R² = 0.00183526153719227

MAGED2

Methylation

Gene

exp

ress

ion

Methylation inhibit the MAGEB2 expression, increase life span

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

-1

-0.5

0

0.5

1

1.5

2

f(x) = 0.0230126000854307 x + 0.0336951391054741R² = 0.000318591896984022

MAGEA11

Methylation

Gene

Exp

ress

ion

0 0.1 0.2 0.3 0.4 0.5 0.6

-1

-0.5

0

0.5

1

1.5

2

f(x) = 0.108849835707722 x + 0.0202142569790082R² = 0.00278903436742295

MAGEA11

Methylation

Gene

exp

ress

ion

(2nd

)0 0.05 0.1 0.15 0.2 0.25 0.3 0.35

-0.8-0.6-0.4-0.2

00.20.40.60.8

11.2

f(x) = 0.257796826810243 x − 0.0226975425081518R² = 0.00471748419011153

MAGEH1

Methylation

Gene

exp

ress

ion

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

-0.8-0.6-0.4-0.2

00.20.40.60.8

11.2

f(x) = 0.0424363004039068 x − 0.0151396248360341R² = 0.000289553744404758

MAGEH1

Methylation

Gene

exp

ress

ion

(2nd

)

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

-1

-0.5

0

0.5

1

1.5

2

2.5

3

f(x) = − 0.220623134850392 x + 0.203730064938955R² = 0.00583066076551353

MAGEA12

Methylation

Gene

exp

ress

ion

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8

-1

-0.5

0

0.5

1

1.5

2

2.5

3

f(x) = − 0.243467016048532 x + 0.206481171986796R² = 0.00611711406918114

MAGEA12

Methylation

Gene

exp

ress

ion

0 0.05 0.1 0.15 0.2 0.25

-2-1.5

-1-0.5

00.5

11.5

22.5

f(x) = − 0.61221159123106 x + 0.230444080072514R² = 0.00178127023472341

MAGED1

Methylation

Gene

exp

ress

ion

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4

-2-1.5

-1-0.5

00.5

11.5

22.5

f(x) = − 0.590415203277728 x + 0.239739452397554R² = 0.0053644833220573

MAGED1

Methylation

Gene

exp

ress

ion

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1-1

-0.50

0.51

1.52

2.53

3.54

f(x) = − 0.309865456222292 x + 0.193432663565709R² = 0.049836553401494

MAGEC1

Methylation

Gene

exp

ress

ion

0 0.2 0.4 0.6 0.8 1 1.2-1

-0.50

0.51

1.52

2.53

3.54

f(x) = − 0.290689706593913 x + 0.176986116537847R² = 0.0400566277976366

MAGEC1

Methylation

Gene

exp

ress

ion

P=0,1274

0 0.2 0.4 0.6 0.8 1 1.2

-2

-1

0

1

2

3

4

5

f(x) = − 0.21447090906437 x + 0.229132717845224R² = 0.00633735605194108

MAGEA10

Methylation

Gene

exp

ress

ion

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1-1

-0.50

0.51

1.52

2.53

3.54

f(x) = 0.0455067210020692 x + 0.00756771853190782R² = 0.00118175847751578

MAGEA8

Methylation

Gene

exp

ress

ion

0 0.2 0.4 0.6 0.8 1 1.2

-2

-1

0

1

2

3

4

5

f(x) = − 0.188259718741881 x + 0.218376838395587R² = 0.00503010879958177

MAGEA10

Methylation

Gene

exp

ress

ion

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4 0.45 0.5

-0.4-0.3-0.2-0.1

00.10.20.30.40.5

f(x) = 0.0933273640141773 x − 0.0211129199951481R² = 0.0082151366026767

MAGEE1

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6

-0.4-0.3-0.2-0.1

00.10.20.30.40.5

f(x) = 0.107593734217629 x − 0.0173905785471191R² = 0.0122702658237978

MAGEE1

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

-0.4-0.3-0.2-0.1

00.10.20.30.40.5

f(x) = − 0.0334696747904668 x + 0.0198944597744082R² = 0.00422362203202798

MAGEA5

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

-0.4-0.3-0.2-0.1

00.10.20.30.40.5

f(x) = − 0.0226295671722427 x + 0.0197762522564552R² = 0.00360536566872149

MAGEA5

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8

-0.4

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.4

f(x) = 0.0288509589232703 x − 0.00771367677995617R² = 0.00481277088125098

MAGEB3

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

-0.4

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.4

f(x) = 0.0489597047768901 x − 0.00915614796898346R² = 0.00671923164040789

MAGEB3

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1

-0.4

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.4

f(x) = − 0.0606129534098957 x + 0.0304217645209539R² = 0.0272165923169282

MAGEA6

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1

-0.4

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.4

f(x) = − 0.0601934752486244 x + 0.0310496514784214R² = 0.0287052612474278

MAGEA6

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8

-0.4

-0.2

0

0.2

0.4

0.6

0.8

f(x) = − 0.018712935240924 x + 0.0157587457655398R² = 0.00157998306351237

MAGEB4

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

-0.4

-0.2

0

0.2

0.4

0.6

0.8

f(x) = − 0.0124204716961998 x + 0.0148455845735156R² = 0.00105791237435238

MAGEB4

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

-0.4

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

0.4

0.5

f(x) = − 0.102770085314559 x + 0.0184116760606217R² = 0.00901767698787026

MAGEE2

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1

-2

-1

0

1

2

3

4

5

f(x) = − 0.211750998624707 x + 0.330089595178982R² = 0.00268894169987732

MAGEA9B

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

-0.3

-0.2

-0.1

0

0.1

0.2

0.3

f(x) = − 0.0132436302631602 x − 0.00613715352620989R² = 0.00211443010051682

MAGEB18

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9

-0.5-0.4-0.3-0.2-0.1

00.10.20.30.4

f(x) = − 0.00164083701411615 x + 0.000818622611243408R² = 1.24996614228046E-05

MAGEC3

Axis Title

Axis Title0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1

-0.5-0.4-0.3-0.2-0.1

00.10.20.30.4

f(x) = − 0.000788509938801673 x + 0.000683347564467422R² = 5.29604166377151E-06

MAGEC3

Axis Title

Axis Title

0 0.05 0.1 0.15 0.2 0.25

-2-1.5

-1-0.5

00.5

11.5

22.5

f(x) = − 0.612211591231062 x + 0.230444080072514R² = 0.00178127023472341

MAGED1

Axis Title

Axis Title

0 0.05 0.1 0.15 0.2 0.25 0.3 0.35 0.4

-1.5

-1

-0.5

0

0.5

1

1.5

f(x) = 0.197843446856895 x + 0.0137069192056319R² = 0.00183526153719227

MAGED2

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6 0.7

-2-1.5

-1-0.5

00.5

11.5

22.5

3

f(x) = − 1.71408218643785 x + 0.441982431309442R² = 0.0785542702933715

MAGED4 (2)

Axis Title

Axis Title

0 0.1 0.2 0.3 0.4 0.5 0.6

-2-1.5

-1-0.5

00.5

11.5

22.5

3

f(x) = − 2.09053988067637 x + 0.419733368735678R² = 0.0500522322492711

MAGED4 (1)

Axis Title

Axis Title

Km5P=0,0703

Km2

Km7

Km1

Km3

Km4

P=0,0734

Km6

P=0.0026

Correlation of methylation and gene expression

(A) Correlation between ID4 expression and ID4 promoter methylation in human breast cancer

For this purpose, a part of the same breast cancer cohort (n = 46) used previously for methylation analysis was re-assessed (Figure 2A ).                                                                                                                                                                                                                                                                                                                                                    We found an increased risk for tumour recurrence in breast cancer patients with ID4 promoter methylation (P = 0.036) compared to patients with lack of ID4 methylation (Figure 2B ).

Methylation headmap of MAGE genes?

MAGEE1 The sequence you input has 13375 nucleotides in length. There are 2 possible CpG island(s) found.

Homo sapiens melanoma antigen family E, 1 (MAGEE1), RefSeqGene on chromosome X. CDS 5279..8152

MAGEE2 There are NO possible CpG islands found.

Homo sapiens melanoma antigen family E, 2 (MAGEE2), RefSeqGene on chromosome X.

MAGEC1Homo sapiens melanoma antigen family C, 1 (MAGEC1), RefSeqGene on chromosome X.

Homo sapiens melanoma antigen family A, 2B (MAGEA2B), mRNA

Homo sapiens melanoma antigen family A, 11 (MAGEA11), RefSeqGene on chromosome X

Homo sapiens melanoma antigen family A, 10 (MAGEA10), transcript variant 1, mRNA

NCBI Reference Sequence: NM_001011543.2

Homo sapiens melanoma antigen family B, 18 (MAGEB18), RefSeqGene on chromosome X

NCBI Reference Sequence: NG_016370.1

Homo sapiens melanoma antigen family C, 3 (MAGEC3), RefSeqGene on chromosome XNCBI Reference Sequence: NG_013272.1

Homo sapiens melanoma antigen family D, 1 (MAGED1), RefSeqGene on chromosome XNCBI Reference Sequence: NG_012559.1

Homo sapiens melanoma antigen family H, 1 (MAGEH1), RefSeqGene on chromosome X

NCBI Reference Sequence: NG_013248.1

Homo sapiens melanoma antigen family D, 2 (MAGED2), RefSeqGene on chromosome XNCBI Reference Sequence: NG_012844.1

Homo sapiens melanoma antigen family B, 4 (MAGEB4), RefSeqGene on chromosome XNCBI Reference Sequence: NG_015870.1

Homo sapiens melanoma antigen family B, 1 (MAGEB1), RefSeqGene on chromosome XNCBI Reference Sequence: NG_016336.1

Homo sapiens melanoma antigen family A, 3 (MAGEA3), RefSeqGene on chromosome X

NCBI Reference Sequence: NG_013244.1

There are 1 possible CpG island(s) found.

Homo sapiens melanoma antigen family A, 6 (MAGEA6), transcript variant 2, mRNANCBI Reference Sequence: NM_175868.1

Homo sapiens melanoma antigen family A, 5 (MAGEA5), RefSeqGene on chromosome X

NCBI Reference Sequence: NG_016417.1

Homo sapiens MAGED4 protein (MAGED4) mRNA, complete cdsGenBank: AF329733.1

There are 2 possible CpG island(s) found.

http://www.urogene.org/methprimer

Homo sapiens melanoma antigen family B, 10 (MAGEB10), RefSeqGene on chromosome XNCBI Reference Sequence: NG_017094.1

There are 1 possible CpG island(s) found.

Homo sapiens melanoma antigen family C, 2 (MAGEC2), RefSeqGene on chromosome XNCBI Reference Sequence: NG_015872.1

Homo sapiens melanoma antigen family B, 2 (MAGEB2), RefSeqGene on chromosome XNCBI Reference Sequence: NG_013246.1

PREDICTED: Homo sapiens melanoma antigen family B, 5 (MAGEB5), mRNANCBI Reference Sequence: XM_293407.7

Homo sapiens melanoma antigen family A, 12 (MAGEA12), RefSeqGene on chromosome XNCBI Reference Sequence: NG_015852.1

Homo sapiens melanoma antigen family A, 9 (MAGEA9), mRNANCBI Reference Sequence: NM_005365.4