mal3.peg1.1 mal3. tip1.

peg1.1 tip1.

mal3.tip1.

peg1.1mal3.tip1.

peg1.1dhc1.

Grallert et al. Supplementary Figure 1

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peg1.1dlc1.dlc2.

tip1. nmt1GFPatb2 20M thiamine 2M thiamine

Grallert et al. Supplementary Figure 2

5m

GFPatb2

Cdc2

atb2nda2

wild type, 25C

wild type, 36C

nmt81GFPatb2, 25C

nmt81GFPatb2, 36C

peg1.1, 25C

peg1.1, 36C

peg1.1 nmt81GFPatb2, 25C

peg1.1 nmt81GFPatb2, 36C

nmt81GFPatb2 + ppeg1.c, 25C

nmt81GFPatb2 + p ppeg1.c, 36C

peg1.1 nmt81GFPatb2 + p ppeg1.c, 25C

peg1.1 nmt81GFPatb2 + p ppeg1.c, 36C

Relative -tubulin level

nmt81GFPatb2, 25C

nmt81GFPatb2, 36C

peg1.1 nmt81GFPatb2, 25C

peg1.1 nmt81GFPatb2, 36C

mal3. nmt81GFPatb2, 25C

mal3. nmt81GFPatb2, 36C

peg1.1 mal3. nmt81GFPatb2, 25C

peg1.1 mal3. nmt81GFPatb2, 36C

peg1.1 mal3., 25C

peg1.1 mal3., 36C

mal3.

nmt81GFPatb2 + pDB20, 25C

mal3. nmt81GFPatb2 + pDB20,, 36C

tip1. peg1.1 nmt81GFPatb2, 25C

tip1. peg1.1 nmt81GFPatb2, 36C

tip1. nmt81GFPatb2, 25C

tip1. nmt81GFPatb2, 36C

tip1., 25C

tip1., 36C

0

1

Grallert et al. Supplementary Figure 3

wild type

peg1.1

tip1.

tip1. peg1.1

mal3.

mal3.peg1.1

tip1. mal3.

tip1. mal3. peg1.1

Grallert et al. Supplementary Figure 4

dhc1.

dhc1. peg1.1

dlc1.dlc2.

dlc1.dlc2. peg1.1

5m

Grallert et al. Supplementary Figure 5

tea1.pkGFP

tea1.pkGFP+

mal3.

tea1.pkGFP

tea1.pkGFP+

peg1.1 tea1.pkGF

P

tea1.pkGFP+

peg1.1 mal3.

tea1.pkGFP

Merged

GFP signa

l

Lectin marker

to identify tea1.pkGFP

cells Brightfie

ld to identify all cells in field

5m

Grallert et al. Supplementary Figure 6

0 min 1 min 2 min 3 min 4 min 5 min

wild type

peg1.1

10 m

16 h Nitrogen starvation > ice/salt bath 20 mins > re-warm to 36°C B

Log phase culture > ice/salt bath 20 mins > re-warm to 36°C A0 min 1 min 2 min 3 min 4 min

wild type

peg1.1

wild type

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dhc1.

After MT reaches cell tipBefore MT

reaches cell tipAs MT curls around

cell tip

Grallert et al. Supplementary Figure 7

Grallert et al. Supplementary Table 1

IH163 972 h- la b stock IH3505 pe g1.1 ura4.D18 leu1.32 h - this s tudy UFY561 mal3::ura4+ ade 6.M210 le u1.32 ura4.D18 his3. h+ this s tudy UFY646 pe g1.1 m al3::ura4+ ade 6.M210 le u1.32 ura4.D18 his3.D h+ this s tudy IH1741 nm t81GFP atb2:kanMX6 le u1.32 h - Garcia et al.

2001 IH1742 nm t1GFPatb2:kanMX6 le u1.32 h- Garcia et al.

2001 IH3379 pe g1.1 n m t81GFP atb2:kanMX6 ura4.D18 h - this s tudy IH3687 pe g1+/pe g1+ nm t81GFP atb2:kan MX6/nmt81GFPatb2:kan MX6 ade 6.M210/ade 6.M216 h -/h+ this s tudy IH4585 pe g1.hph:MX6/peg1+ n mt81GF Patb2:kanMX6/nmt81GF Patb2:kanMX6

ade 6.M210/ade 6.M21 6 h-/h+ this s tudy

IH4586 pe g1.1/pe g1.1 n mt81GFPatb2:kan MX6/nmt81GF Patb2:kanMX6 ade 6.M210/ade6.M216 h -

/h+ this s tudy

IH3836 pe g1.1/pe g1+ nmt81G FPatb2:kanMX6/nm t81GFPa tb2:kanMX6 a de6.M210/ade 6.M21 6 h-

/h+ this s tudy

IH3704 tip1::kan MX6 nmt81GF Patb2:kanMX6 ura4.D18 h - this s tudy IH4587 tip1::kan MX6 nmt1GFP atb2:kanMX6 ura4.D18 h - this s tudy IH3703 pe g1.1 tip1::kanMX6 nmt81GF Patb2:kanMX6 leu1.32 h is2 h+ this s tudy IH3651 mal3::ura4+ nmt81GF Patb2:kanMX6 his3 le u1.32 ade 6 this s tudy IH3577 pe g1.1 m al3::ura4+ nmt81G FPatb2:kanMX6 this s tudy IH4901 pe g1.C9m yc:kan MX6 h - this s tudy IH3907 tip1.C6 HA:kanMX6 h - this s tudy IH4909 pe g1.C9m yc:kan MX6 t ip1.C6HA:kan MX6 h - this s tudy IH3613 tea1.CpkGFP:ura4+ ura4.D18 le u1.32 this s tudy IH3591 pe g1.1 tea1.CpkGFP:ura4+ ura4.D18 le u1.32 h - this s tudy IH4124 pe g1.C9m yc:kan MX61 tea1.CpkGFP:ura4+ ura4.D18 this s tudy IH3499 mal3:CpkGFP:ura4+ ura4.D18 le u1.32 his3 .d1 a de6.M210 h - this s tudy IH3502 pe g1.1 m al3:CpkGFP:ura4+ ura4.D18 le u1.32 his3 .d1 ade 6.M210 h - this s tudy IH3893 mal3:CpkGFP:ura4+ tip1.C6H A:kanMX6leu 1.32 ura4.D18 h- this s tudy IH4125 pe g1.C9m yc:kan MX6 m al3:CpkGFP:ura4+ ura4.D18 le u1.32 h- this s tudy IH3638 tip1.C4GF P:kanMX6 h - this s tudy IH3680 pe g1.1 tip1.C4G FP:kanMX6 h - this s tudy IH3732 tip1.C4GF P:kanMX6 m al3::ura4+ ura4.D18 le u1.32 h- this s tudy IH4207 pe g1.N2GFP:ura4+ ura4.D18 h- this s tudy IH4236 pe g1.N2GFP:ura4+ mal3::ura4+ ura4.D18 leu1.32 h - this s tudy IH4233 pe g1.N2GFP:ura4+ tip1::kan MX6 ura4.D18 leu 1.32 h - this s tudy IH5297 pe g1.N2GFP:ura4+ tip1::kan MX6 m al3::ura4+ te a1::ura4+ ura4.D18 this s tudy IH3746 pe g1.C4GFP :kan MX6 h - this s tudy IH3797 pe g1.C4GFP :kan MX6 nmt81GFPatb2:kan MX6 h - this s tudy IH5536 pe g1.N2GFP:ura4+ nm t81GFP atb2:kanMX6 ura4.D18 h- this s tudy IH4180 pe g1.1 leu1::nmt81 p e g1+:ura4+ ura4.D18 h - this s tudy IH3897 dhc1::ura4+ nmt81GF Patb2:kanMX6 leu1.32 ura4.D18 ade 6.M216 h- this s tudy IH3899 pe g1.1 d hc1::ura4+ nmt81GF Patb2:kanMX6 leu1.32 ura4.D18 h- this s tudy IH4213 mal3::ura4+ tip1::kanMX6 nmt81GFPatb2:kan MX6 le u1.32 ura4.D18 h- this s tudy IH4370 pe g1.1 m al3::ura4+ tip1::kan MX6 nmt81GFPatb2:kan MX6 le u1.3 2 ura4.D18 h - this s tudy IH5028 dhc1::ura4+ m al3::ura4+ tip1::kanMX6 nmt81GFPa tb2:kanMX6 le u1.3 2 ura4.D18 h - this s tudy IH5296 pe g1.1 dhc1::ura4+ mal3::ura4+ tip1::kanMX6 nmt81GFPatb2:kan MX6 leu 1.32 ura4.D18 h- this s tudy IH4346 pe g1.N6pk:ura4+ ura4.D18 h - this s tudy IH5229 pe g1.N6pk:ura4+ dhc1.c6HA :kan MX6 ura4.D18 h - this s tudy IH5103 dhc1.C6HA:kan MX6 h - this s tudy IH5018 dlc1::ura4+ dlc2::kan MX6 nmt81GF Patb2 le u1.32 ura4.D18 this s tudy IH5021 dlc1::ura4+ dlc2::kanMX6 peg1.1 nmt81G FPatb2 ura4.D18 h - this s tudy IH5478 pe g1.N2GFP:ura4+ s id4.tdTomato:hph MX6 ura4.D18 this s tudy IH5484 pe g1.N2GFP:ura4+ cnp1.cher ry:kanMX6 le u1.32 ura4.D18 h- this s tudy IH3901 cdc11.123 m ad2::ura4+ leu 1.32 ura4.D18 h- this s tudy IH3927 tip1::kan MX6 cdc1 1.123 m ad2::ura4+ le u1.32 ura4.D18 h - this s tudy IH3699 pe g1.1 cdc11.123 m ad2::ura4+ leu1.32 ura4.D18 h - this s tudy

Grallert et al. Supplementary Table 2

Supplementary Table 2: movies in supplemental information online Movie

number

Subject

Movie 1 wild type (peg1+) atb2.nmt81GFP normal mitosis

Movie 2A peg1.1 atb2.nmt81GFP defective mitosis

Movie 2B peg1.1 atb2.nmt81GFP defective mitosis

- note that once it gets to the point the anaphase B in the cell on

the rigt is apparently normal

Movie 3 peg1.1 atb2.nmt81GFP interphase microtubule dynamics

Movie 4 wild type (peg1+) atb2.nmt81GFP interphase microtubule

dynamics

Movie 5 tip1. atb2.nmt81GFP

Movie 6A tip1. pnmt3GFPatb2 20 M thiamine

Movie 6B tip1. pnmt3GFPatb2 2 M thiamine

Movie 7 tip1. peg1.1 atb2.nmt81GFP

Movie 8 mal3. atb2.nmt81GFP

Movie 9 mal3. peg1.1 atb2.nmt81GFP

Movie 10A peg1.C4GFP atb2.nmt81GFP

Movie 10B peg1.N2GFP atb2.nmt81GFP

Movie 11 dhc1. atb2.nmt81GFP

Movie 12 dhc1. peg1.1 atb2.nmt81GFP

Movie 13 dlc1. dlc2. atb2.nmt81GFP

Movie 14 dlc1. dlc2. peg1.1 atb2.nmt81GFP