Management of carriers of hereditary predisposition to breast cancer
Marc Abramowicz
Medical Genetics, ULBCentre des tumeurs ULB – Erasme / Bordet
Cancer Genetics
• In tumors (tumor DNA = somatic DNA) : Molecular pathology; tumorigenesis routes.
– Genetic– Epigenetic
• In patients (constitutional DNA = germ-line DNA) :Cancer risk profiling, from inherited mutations/polymorphisms
10-12% women will develop breast cancer
No genetic factor
Major genetic factor (high penetrance gene)
All breast cancer cases (> 10% women)
Minor genetic factor(s)
Hereditary breast/ovarian cancer
• Genetically heterogeneous– BRCA1– BRCA2– Others (?) and extended syndromes: Li-Fraumeni, Cowden, …
• Penetrance incomplete and age- (and gender-) dependent 50 – 80 %, breast; 15– 60 %, ovary
• Phenocopies: chance coincidence of cancer in same families
• No specific cancer phenotype
Br+ov: >80% of multiple familial casesBr only: >50% of multiple familial cases (Ford et al 1998)
Hereditary breast and ovarian cancer
5-10% of breast cancers are inherited
– Familial occurence, mendelian pattern• Heterozygous for one single gene
– multiples primary tumours– Early onset (mean age, breast: 42 yrs)
Penetrance, BRCA1 – linked breast cancer
Struewing et al. 1997, NEJM 336: 1401-8.
Penetrance, BRCA2 – linked ovarian cancer
Struewing et al. 1997, NEJM 336: 1401-8.
Penetrance of breast cancer
• Biased in families ascertained for high incidence of breast cancer• Meta-analysis of 10 studies < high-risk clinics and population-based
settingChen & Parmigiani JCO 2007
Cumulative incidence by age 70
95% Confidence Interval
BRCA1 .57 .47 - .66
BRCA2 .49 .40 - .57
Penetrance of breast cancer
• Biased in families ascertained for high incidence of breast cancer• Meta-analysis of 10 studies < high-risk clinics and population-based
settingChen & Parmigiani JCO 2007
• But depends on low-penetrance alleles too: some effect of family history even after exclusion of BRCA mutation
Cumulative incidence by age 70
95% Confidence Interval
BRCA1 .57 .47 - .66
BRCA2 .49 .40 - .57
BRCA1 ≠ BRCA2but no difference in clinical care as of today
• BRCA1 activates transcription of ER gene ESR1Hosey 2007 J Natl Canc inst
• BRCA1 required for differentiation of stem cell, and progenitor cell (ER-) into luminal cell :Liu-S et al 2008 PNAS
• … and other cellular functions
Cf: BRCA1 carrier tumors often ER- (75%); many Basal-like type ER-, PR-, HER2- (44%)Lakhani et al 2005 Clin Canc Res
BRCA2 carrier tumors mostly ER+ (75%), ductal carcinomas, luminal typeFoulkes 2004 Clin Canc Res
GENETIC TESTING AND GENETIC COUNSELING
Genetic analysis BRCA1&2
• Labor-intensive (3 months) ; expensive• Mutation detection efficacy 70 %
– Some mutations in introns, in gene promoters,…
– BRCA3 ?
• Psycho and social impact of testing• False +, false -. False reassurance.
=> Not performed in each patient with breast or ovarian cancer(ASCO 1996; ASCO 2003)
Hereditary breast/ovarian cancer
Care management of this patient must include DNA sampling, in order to counsel her daughter appropriately in the future.
DNA analysis and genetic counseling
• If hereditary cancer plausible (Proba BRCA mutation > 10%)– 2 women < 50 yrs (any age if bilateral) (1st degree, 2nd if paternal)– 1 breast < 50 yrs + 1 ovary, any age– 4 women, breast only– 1 woman breast + ovary– 1 woman < 35 yrs
• Test affected relative first. With informed consent. Via genetic counseling.
• In women with cancer history: – Breast recurrence risk; ovarian risk; family implications
• In asymptomatic relatives: PGT (Presymptomatic Genetic Testing)– NO SAMPLING AT FIRST VISIT. – Counsel on: Breast risk; ovarian risk; Medical options; Family implications– Psycho support. Anticipate results of PGT, and adjust– Duplicate DNA sampling
DNA analysis and genetic counseling
• If hereditary cancer plausible (Proba BRCA mutation > 10%)– 2 women < 50 yrs (any age if bilateral) (1st degree, 2nd if paternal)– 1 breast < 50 yrs + 1 ovary, any age– 4 women, breast only– 1 woman breast + ovary– 1 woman < 35 yrs
• Test affected relative first. With informed consent. Via genetic counseling.
• Reassure 50% of first-degree relatives• In mutation carriers, provide risk management strategy
– optimum = ?– Bilateral prophyllactic ovario-annexectomy:
Ov. Ca risk, Br.Ca risk, overall mortality– Prophyllactic radical mastectomy ?– Breast surveillance
*
*Mutation tronquante pLys339ArgfsX2 de BRCA1, Prévoir ovariectomie chez III.4, tester II.4
II.3 K sein 48 ans, DCD 50II.4 K sein 35 ans, ovaire 51 ansIII.4 K sein 39 ans mutation g30877delA pLys339ArgfsX2 de BRCA1
MANAGEMENT OF CARRIERS
Prophyllactic mastectomy in asymptomatic carrier ?
• Reduces risk by >95% only if total (not subcutaneous)– Rebbeck et al J Clin Oncol 2004 (PROSE study), and references therein
• No evidence for significant survival benefit as compared with surveillance only – No level 1 evidence, unclear other evidence
– Prospective, randomized, 2-blind study unfeasible
• No effect on ovarian risk.
=> Only in carefully selected, fully informed, mastectomy-requesting patients
=> Optimal age for procedure = ?
Age-related penetrance
Bilateral mastec if unilateral cancerin BRCA1or2 carrier ?
• Reduces by 90% risk of recurrence, ipsi or heterolateral (same as in asymptomatic mutation carrier)
• Does not reduce mortality as compared with surveillance–only groupVan Sprundel et al. Br J Cancer 2005
Guidelines in asymptomatic BRCA1/2 carriers: (1) OVARIES
• Risk-Reducing Salpingo-Oophorectomy at 35-50 yrs:
– Ov.Ca risk by >95%Kauff et al 2002 NEJM; Rebbeck et al. 2002 NEJM; Rutter et al. 2003 J Natl C Inst; Eisen et al. 2005 JCO
– Br.Ca risk by 50% if BRCA1 mutation Eisen et al. AJHG 2000; Kauff et al. NEJM 2002; Rebbeck et al. NEJM 2002;
– overall mortality Rebbeck et al. NEJM 2002; Domchek et al. Lancet 2006
• But unclear if same benefit in BRCA2 as in BRCA1(Kauff 2008 JCO)
Guidelines in asymptomatic BRCA1/2 carriers: (2) BREAST
• Prophyllactic total mastectomy ? Unclear benefit in terms of survival– Br ca risk by 90% (95% if +RRSO) Rebbeck et al 2004 JCO
• Breast surveillance: – monthly self-exam? No proven benefit– mammograms ± US
Mammograms reduce breast cancer death by 22% after 50 yrs (Humphrey 2002 Ann Int Med)BUT: denser breast / faster doubling size in younger women
Ultrasounds improve detection rate (Kuhl 2005 JCO)– clinical breast exam by experienced MD: improves detection in
some studies (Warner 2004 JAMA) – MRI (Kriege et al NEJM 2004): more Ss, less Sp => likely cost
effective if high a priori risk: BRCA1; BRCA2 with dense breasts• Tamoxifen? Only BRCA2. Raloxifen, AIs? Unclear
(King et al. 2001 JAMA; Robson & Offit 2007 NEJM)
Guidelines in asymptomatic BRCA1/2 carriers (3)
• optimum = ?• Bilat prophyl salpingo-oophorectomy at 35-50 yrs:
– Ov.Ca risk by >95%Kauff et al 2002 NEJM; Rebbeck et al. 2002 NEJM; Rutter et al. 2003 J Natl C Inst; Eisen et al. 2005 JCO
– Br.Ca risk by 50% if BRCA1 mutation Eisen et al. AJHG 2000; Kauff et al. NEJM 2002; Rebbeck et al. NEJM 2002;
– overall mortality Rebbeck et al. NEJM 2002; Domchek et al. Lancet 2006
• Breast surveillance: monthly self-exam; mammograms ± US; clinical breast exam. MRI. (Kriege et al NEJM 2004)
• Prophyllactic total mastectomy ? Unclear benefit => only in carefully selected, fully informed patients
Trends
• Identify low-penetrance alleles
• Risk profiling ?• Targeted prevention ?• Pathway-specific
therapies• Pharmacogenetics
SOME MOLECULAR DATA
Major (monogenic) hereditary predisposition to breast cancer
Robson & Offit 2007
Major (monogenic) and minor (polygenic) hereditary predisposition to breast cancer
Robson & Offit 2007
A cellular pathway of DNA repair and chromosome integrity
Walsh & King 2007 Cancer cell
What does this teach us?
• Low penetrance alleles modulate high-penetrance genes (eg Antoniou 2008 AJHG)
• BRCA1 deficient cell lines are sensitive to Xrays; Etoposide; Bleomycin: double-strand DNA breaks, and Platinum drugs: DNA cross-links
=> platinum clinical trials in BRCA1-deficient tumor patients
What does this teach us?
• Low penetrance alleles modulate high-penetrance genes (eg Antoniou 2008 AJHG)
• BRCA1 deficient cell lines are sensitive to Xrays; Etoposide; Bleomycin: double-strand DNA breaks, and Platinum drugs: DNA cross-links
=> platinum clinical trials in BRCA1-deficient tumor patients
• Poly-ADP-ribose polymerase (PARP) required at initial step of DNA repair
=> PARP inhibitors in BRCA1 or 2-deficient tumor patients
LOW-PENETRANCE ALLELES
Majority ? of breast cancers
• Some susceptibility stems from combination of several genes, each with low penetrance– Twin studies: concordant MZ ~25%
(Ahlbom et al 1997 JNCI; Baker et al 2005 Biometrics)
– SNPs; CNVs (?); polygenic– A few genes known in 2008… more data are coming
• CHEK2, ATM, PALB2, BRIP1; …, FGFR2,
– Some of the same genes modify severity of BRCA1&2 course– No routine testing
• Environment (hormones, diet, other factors)• Epigenetic effects? Already in utero ??
Families with > 4 cases of breast cancer
The most important conclusion from this analysis is that a large proportion, perhaps the majority, of families with five or fewer cases of breast cancer and no ovarian or male breast cancer cases are not due to either BRCA1 or BRCA2.
Am J Hum Genet 62:676-9, 1998
Familial breast cancer: 58209 cases + 101986 controls Collaborative group, Lancet 358:1389-99, 2001
• Most women with breast cancer have no affected relative; most women with affected relatives will not develop br ca.
• If cancer, not at young age. Age of relative at Δ has little effect.
Trends
• Identify low-penetrance alleles
• Risk profiling ?• Targeted prevention ?• Pathway-specific
therapies• Pharmacogenetics
Age-related penetrance