Manual Quick Ray Eng

Quick-Ray™ User manual

www.unitma.com

Tissue array using Quick-Ray™

Unitma Co., Ltd., 3F Chungmyeong B/D, 224-8, Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea TEL 82-2-420-0070. FAX 82-2-420-9797 E-mail [email protected] http://www.unitma.com

NOTE

This manual describes the instruction of Quick-RayTM.

Review this manual to avoid injury and prevent damage to this product or any products connected to it before you use

Quick-RayTM. To avoid potential hazards, use this product only as specified in this manual.

This documentation contains information and warnings that must be followed by the user to ensure safe operation and to

maintain the product in a safe condition.

Customer shall be responsible for paying all shipping charges, duties, taxies, and any other charges for any failure or

damage or injury caused by improper use or improper or inadequate maintenance and care. Unitma Co., Ltd. shall not

be obligated to furnish service under this case a) to repair damage resulting from attempts by personnel other than

Unitma Co., Ltd. representatives to install, repair or service the product; b) to repair damage resulting from improper use

or connection to incompatible equipment; c) to repair any damage or malfunction caused by the use of non-Unitma Co.,

Ltd. supplies; or d) to service a product that has been modified or integrated with other products when the effect of such

modification or integration increases the time or difficulty of servicing the product.

Copyright© Unitma Co., Ltd. All rights reserved. Licensed products are owned by Unitma Co., Ltd., and are protected by

national copyright laws and international treaty provisions. Unitma Co., Ltd products are covered by patents, issued and

pending. Specifications and price change privileges reserved.

QuickRay is registered trademarks of Unitma Co., Ltd.

Contacting Unitma Co., Ltd. UNITMA Co., Ltd. 3F, Chungmyeong B/D, 224-8,

Jamsil-Dong, Songpa-Ku, Seoul, 138-220, Korea

Tel: +82-2-420-0070

Fax: +82-2-420-9797

http://www.unitma.com

E-mail: [email protected]



IMPORTANT NOTES • Explanation of Symbols used

• SAFETY NOTES

To avoid personal injury

Use the Quick-RayTM and accessories according to the instructions in this manual

WARNING: WARNING indicates an injury hazard not immediately accessible

as you read this symbol. It calls attention to an operating procedure, practice,

or like that, if no correctly performed or adhered to, could result in personal

injury or death. Do not proceed beyond a WARNING notice until the

indicated conditions are fully understood and met.

BIOHAZARD WARNING: BIOHAZARD WARNING indicates an injury hazard

not immediately accessible as you read this symbol.

Biohazard(Infectious agent): A type of micro-organism, bacteria, mold parasite

or virus which normally causes, or significantly contributes to the cause of,

increased morbidity or mortality of human beings.

Read the following safety notes to avoid injury and prevent damage to this

product or any products connected to it.

• Be careful not to pinch your fingers when closing the wooden case.

• Keep the Quick-RayTM and accessories out of the reach of small children.

• Be careful for your fingers or hand not to be scratched by the needle of tip

when assembling or disassembling the tip or the probe, or when cleaning the

tip.

• Use the Quick-RayTM and accessories according to the instructions in this

manual. No authorization for the analysis or modification of the Quick-RayTM is

provided.

• Do not use accessories that are not supplied or recommended by UNITMA.

• Contact UNITMA for repairing or service or buying accessories.



Do not use the donor block or a sample with a biological hazard or a chemical hazard.

Handle biohazards.

• Do not use the donor block or a sample with a biological hazard or a chemical

hazard that can impact human health.

• Working with infectious material requires the following precautions.

a. Limit access to areas where experiments with infectionus specimens are in

progress.

b. Clearly label areas where biohazards are in use and designate specific

areas where biohazards are routinely used, using this symbol (black on red

background):

c. Wear lab coat, gloves and safety glasses to prevent contamination from the

infectious specimen, and remove them when leaving the work area.

d. Decontaminate work surfaces once per day and after any spill of viable

specimen.

e. Eating, drinking and applying cosmetics are not permitted in the work area.

f. Wash hands after handling viable specimens before leaving the lab.

g. Transport contaminated materials in a red biohazard bag which has been

placed in a labeled, leak-proof container.



Biohazard Determination.

• Lab Biosafety Level Criteria.

The following guidelines can be used by all laboratory personel.

a. Biosafety Level 1 is appropriate for undergraduate and secondary

educational training and teaching laboratories and/or other facilities in which

work is done with defined and characterized strains of viable microorganisms

not known to cause disease in healthy adult humans.

b. Biosafety Level 2 is applicable to clinical, diagnostic, teaching and other

facilities in which work is done with the broad spectrum of indigenous

moderate-risk agents present in the community and associated with human

disease of varying severity.

c. Biosafety Level 3 is applicable to clinical, diagnostic, teaching, research, or

production facilities in which work is done with indigenous or exotic agents

where the potential for infection by aerosols is real and the disease may have

serious or lethal consequences.

d. Biosafety Level 4 is applicable to work with dangerous and exotic agents

which pose a high individual risk of life-threatening disease.

• Practices and Techniques, Safety Equipment Biosafety

Level Practices andTechniques Safety Equipment Facilites

1 Standard microbilogical practices

None: primary containment provided by adherence to standard laboratory practices during open bench operations.

Basic

2

Level 1 practices plus: Laboratory coats; decontamination of all infectious wastes; limited access; protective gloves and biohazard warning signs as indicated.

Partial containment equipment (i.e., Class I or II Biological Safety Cabinets) used to conduct mechanical manipulative procedures that have high aerosol potential that may increase the risk of exposure to personnel.

Basic

3 Level 2 practices plus:special laboratory clothing; controlled access.

Partial containment equipment used for all manipulations of infectious material.

Containment

4 No facilities available on this campus at this time.

Maximum containment equipment.

Maximum Containment



Tissue Microarray ?

"Tissue Microarray (TMA)" is the technology to attach the individual tissue cores in one slide.

The TMA consists of cylindrical paraffin embedded tissues (cores) that are acquired from donor

blocks. "The donor block" is a standard tissue block from surgical pathology, autopsy or research

material. Donor blocks are marked on both slides and tissue blocks to identify morphologically

representative areas of interest. The tissue cores are extracted from the donor block and

inserted into a recipient block. Tissues can be inserted with up to 1000 tissue cores in a recipient

block by using a precise instrument or tool. Thin cross-sections cut from the recipient block (TMA

block) with a microtome are placed onto standard slides for in situ analysis. One TMA block can

generate between 100 and 500 sections.

These TMA technology can attach tissues of several patients or animals on only one slide, and

compare analysis on several genes and protein expression of same tissue, and execute analysis

under same condition. So, it reduces reagent and time, and expenses to 1/60, and increases

research effectiveness. TMA is used widely in recent pathological research analyses, and it is

applicable to most methods used as tissues like Immunohistochemistry, in situ hybridization,

FISH, in situ PCR and so on Tissue.

But the manufacturing of TMA is a critical step in the success of the technology. This creates

tremendous obstacles and impediments for the pathologists to fully utilize the power of TMAs.

Quick-Ray™, the Patented Technologies of UNITMA, overcame these road blocks and

decreased the current time to create Block and Slide in innovative way by providing solutions to

empower the pathologist to easily set up their TMAs with ready-to-go and pre-formed recipient

blocks.



Quick-Ray™ SYSTEM

• Minimized size arrayer comparing with the conventional products

• Portable, easy to carry and to make the array block anytime and anywhere

• Easy to handle. Inexperienced pathologist can handle easily in the lab.

• Simple developing procedure

• Easy to create the various sized block with using disposable bases

• Steel array block and cassette array block are available

• Low cost to purchase

TISSUE MICROARRAY SET

• Quick-Ray™

Weight : 140g

Length : 15.5Cm

• Tip & Recipient block

core size

∅ 1mm

∅ 2mm

∅ 3mm

∅ 5mm

• Guide for 1mm recipient block

Quick-RayTM

Recipient block

Tip

Base Mold



CONSUMABLES

• Disposable-base (Recipient block) • Cassette & Base mold

∅ 5mm x 20 well

∅ 3mm x 30 well

∅ 2mm x 60 well

∅ 1mm x 120well

APPLICATION

• Special stain

• Immunohistochemistry

• in situ hybridization

• FISH

• in situ PCR etc.



PROCEDURE • How to build

1. Place the reference slide and the donor block on

microscope stage for position marking with an oil pen.

2. Extract the marked tissue from the donor block by using the Quick-Ray™ needle.

1) Place the donor block on a horizontal and flat table. 2) Hold the Quick-Ray™ in your hand and tighten your grip. 3) Hold the Quick-Ray™needle perpendicular to the marked position of the donor block. 4) Insert the Quick-Ray™ needle into the donor block at the proper depth of 5mm slowly. (Don’t insert it quickly and too deep to prevent to damage the donor block and the Quick-Ray™ needle.)

* Quick- Ray™ needle’s depth: 5mm * Incubate the easily breaking donor block in a heating oven or a chamber at 37~40℃ for 15

~ 20 minutes.

3. Deliver the extracted tissue into the corresponding holes of the recipient block that were pre-

made by UNITMA, with Quick-Ray™ needle. 1) Place the recipient block on a horizontal and flat table. 2) Hold the Quick-Ray™ needle with the extracted tissue perpendicular to the corresponding holes of the recipient block. 3) Inject the extracted tissue (core) into the corresponding holes of the recipient block at the proper depth of 4mm by pushing the Quick-Ray™ plunger slowly. 4) Adjust the height of all the core with a same settings by pressing the top of all the core with a flat side of a thing.



4. Put the recipient block into embedding mold with cutting section faced down and incubate it in oven at about 70℃ for 30~60 minutes.

(The top side of the recipient block in ‘Step 3’ will be cutting section.)

5. Take out the recipient block when completely transparent. ⇒ Embedding

Step 1 for Embedding Step 2 for Embedding Step3 for Embedding

6. Solidify the block in cold plate.

7. Cutting (about 4㎛)



• How to use the guide 1. Put the recipient block into the prop hole at bottom side.

2. Fit the 1mm guide on the prop.

3. Extract the marked tissue from the donor block by using Quick-Ray™ needle.

4. With fixing the prop by hand, insert the Quick-Ray™ needle into the guide hole, and put the

extracted tissue into the recipient block hole by pushing the knob down.

5. Remove the guide and recipient block from the prop.



6. Put the recipient block into embedding mold with cutting section faced down and incubate it in oven

at 60˚C for 30 minutes

7. Take out the recipient block when completely transparent

⇒ Embedding

8. Solidify the block in cold plate

9. Cutting

* In case of using 2, 3 and 5mm recipient block, insert the extracted tissue directly into the recipient

block without the guide by pushing the Quick-Ray™ knob down.



BLOCK & SLIDE



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Manual Quick Ray Eng

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