SCHOOL OF ALLIED HEALTH SCIENCES
D. Y. PATIL UNIVERSITY
(established under Section-3 of UGC Act.1956 vide notification no. F. 9.21/2000.U.3
(dated 20.06.2002 of the Govt. of India)
Master of Science in Medical Laboratory Technology:
M.Sc. MLT
2 YEARS FULL TIME DEGREE PROGRAMME
CURRICULUM
Sector-7 Dr. D. Y. Patil Vidyanagar, Nerul, Navi Mumbai.
Tel: 91-22 27702218 Email.:[email protected] Web:-www.dypatil.edu
Programme Name: M.Sc. Medical Laboratory Technology
Programme Objectives:
To provide in-depth knowledge in the specific modality of Medical Technology and prepare
the students for leadership roles in hospitals/ diagnostic labs/ research centers/ healthcare
industry and provide professional growth to the students in the following specializations:
Cardiac Care
Respiratory Therapy
Imaging Sciences
Clinical Laboratory
Dialysis & Renal Technology
OT &Anaesthesia Technology
Programme Outcome:
Medical Lab Technology offers opportunity to have significant impact on patient
care. These professionals can find themselves employed in private laboratory, hospitals,
blood donor centres, minor emergency centres, clinics and doctor’s office. They can also
find jobs in crime laboratories, research facilities, pharmaceutical companies, universities,
and military.
Programme Specific Outcomes:
Students will acquire necessary knowledge and skills to work as full-fledged lab
technologists capable of collecting and storing samples, analyzing them and creating
reports based on the sample for further analysis by a doctor.
Students will have knowledge of elements of blood bank management, materials
management, supply chain management as well as lab information system management.
Students will be skilled to clean and maintain lab equipment, manage biomedical.
Professionally competent
Possess commitment to lifelong learning
Exhibite sense of commitment to the ethical and human aspects of patients care.
Recognize the role of the clinical laboratory technician in the assurgency of quality health
care.
TEACHING AND EXAMINATION SCHEME
PROGRAMME STRUCTURE: M.Sc. Medical Laboratory Technology
DURATION OF PROGRAMME -SEMESTERS:
S.No.
Semester/ year
Course Teaching Scheme(Hours) Examination scheme(Marks)
Theory (hrs)
Practical (hrs)
Clinical Postings( hrs)
Total Theory Practical Total
Max 100
100 200
MIn 50 50 100
1 1st Year
Clinical
Pathology
92
1600
160
1852
Paper-I
Max 100
100 200
MIn 50 50 100
Clinical
Biochemistry
Paper-II Max 100
100 200
MIn 50 50 100 Clinical
Microbiology
Paper-III Max 100
100 200
MIn 50 50 100 2 2nd
year Clinical
Pathology
92
1600
160
1852
Paper-I Max 100
100 200
MIn 50 50 100 Clinical
Biochemistry
Paper-II Max 100
100 200
MIn 50 50 100 Clinical
Microbiology
Paper-III Max 100
100 200
MIn 50 50 100
1st year
COURSE-CLINICAL BIOCHEMISTRY
Course Objective:
By the end of the course the students should be able to demonstrate advance
knowledge and understanding in the course areas.
To provide students with the skills required to succeed in graduate school, The
chemical industry or professional school
Course Outcomes
Understand in details the structure and physio chemical properties of carbohydrates
from monosaccharide to polysaccharide.
Too describe the physiology of the carbohydrate digestion in mammals.
Understand the difference between the water soluble and fat soluble vitamins and the
key role in the metabolism.
Course Syllabus
Chemistry of Carbohydrates
Definition and Function
Classification
Isomerism of Monosaccharide’s
Properties of Monosaccharide’s
Modified Monosaccharide’s
Disaccharides
Polysaccharides
Chemistry of Proteins
Definition, function of proteins
Classification of Amino acids
Properties of Amino acids
Classification and properties of proteins
Structural organization of proteins
Chemistry of Lipids
Definition and functions of lipids
Classification of Lipids
Properties of Lipids
Enzymes
Classification of Enzymes
Factors affecting enzyme activity
Inhibitors
Specificity
Enzyme Kinetics
Enzyme in clinical diagnosis
Clinical significance ,principles of estimation
Bilirubin General types and jaundice
Liver function test
Insulin tolerance test
Xylose absorption test
Analysis of calculi
Urine chemistry
Automation in urine chemistry
Physical and Chemical examination of Urine samples.
Common qualitative and quantitative test of urine
Clearance test for urine function
Electrolytes
Sodium,potassium,chloride,CO2 (HCO3)
Total and ionized calcium
Magnesium
Blood gases and pH,carboxyhemoglobin,CO,Met Hb,O2saturation
Disorders of carbohydrate metabolism
Abnormalities of proteins in plasma
Disorders of plasma lipids and lipoproteins
Blood collection procedures –theory of anticoagulation.
Biomedical Techniques
Methods of qualitative and quantitative of biomolecules
Centrifugation Techniques
Qualitative methods –Principles and applications
Isotopes-Detection and measurement of radioactive isotopes
Laboratory Management
Preparation of operating budgets of financial management of laboratories
Cost-analysis: justification of providing new services or rejecting existing ones
Laboratory design: Designing laboratories for different types and size of
institution; selection of equipment and system for the laboratory.
Laboratory safety:Fire,chemical,radiation and infection control
Maintenance of record: Procedures manuals ward manuals, quality control
programs.
Professional ethics
Public relations, hospital and community
Basic clinical epidemiology
Laboratory data processing
COURSE-CLINICAL PATHOLOGY
Course Objectives
Provide knowledge technical skills and experience in clinical pathology. This includes
developing pathologic process and skills needed to interpret data.
Promote effective communication and sharing of expertise with peers and collages.
Course Outcome
After completion of the course the technician will be able to explain the pathologic
process and apply to individual patients.
Analyze the laboratory clinical data.
Successfully manage laboratory
Course Syllabus
Introduction to Pathology : History, branches and scope
Reversible and Irreversible cell injury, types of necrosis and degeneration
Inflammation- Acute & Chronic, definition, types and clinical importance
Neoplasia- Definition, difference between benign & malignant neoplasia
Lab diagnosis of cancer
Granulomatous inflammation, tuberculosis, syphilis, actinomycosis, leprosy.
Autoimmune & HIV- Routes of transmission, clinical features, precautions and
diagnosis.
Infectious diseases: Typhoid, Amoebiasis, cholera.
Hepatitis viruses and vaccination.
Immunology: cell involved, types of hypersensitivity.
Introduction to Histopathology- Receiving of specimen, grossing techniques, tissue
processing & staining with special stains.
IHC: Different stains, introduction, utility and methods.
Microtoming, hot water bath.
Frozen section: cryostat techniques and utility.
Mounting technique & preservation of slides/specimen & maintenance of records and
medicolegalimpt.
Discarding of histopath specimen & biomedical waste.
Museum techniques- Importance of preservation of specimen.
Introduction to hematology- blood collection & anticoagulant bulbs.
Fixation & processing & routine staining of PBS and different stains.
Hemoparasites- Malaria & filarial.
Anemia- Iron deficiency anemia, megaloblastic anemia, hemolytic anemia and
aplastic anemia.
Hematopoiesis, bone marrow examination, methods of staining.
Lab diagnosis of thalessemia, sickle cell anemias and Hb electrophoresis.
Leukemias- definition, types & diagnosis.
Non-neoplastic WBC disorders. Different types of leucopoenia, leucocytosis,
leukemoid reaction.
Platelet disorders & bleeding disorders.
Plasma cell disorders.
Automation in haematology.
Procedure of FNAC-uses, techniques, slides prepared and stains used.
Pap smear- uses, technique, preparation and staining.
Liquid based Pap study- uses, procedure, advantages & disadvantages.
Urine examination- physical, chemical and microscopy.
Automation in urine analysis- advantages & disadvantages.
Processing of body fluids- CSF, Pleural & ascitic fluid, synovial fluid.
Semen analysis- collection, processing & all tests.
Sputum- collection, examination, gram stain, ZN stain.
Basic principles of immune haematology and blood grouping, principles of ABO/Rh
grouping and factors affecting result.
Collection of donor blood, processing and storage.
Cross matching and screening of transfusion transmitted diseases, coombs test and
investigation.
Transfusion reaction.
Blood components-preservation, separation and instruments used.
Blood camp organization- preservation, seapartion and instruments used.
Automation in blood bank.
Record maintenance and FDA regulation.
Discarding of blood samples- HIV/HBsAg/HCV.
National accreditation for testing and calibration laboratories(NABL).
Cleaning of glassware, biomedical waste management, quality control & quality
assurance.
Pathological & medicolegalautopy: uses, importance & precautions.
COURSE-CLINICAL MICROBIOLOGY
Course Objectives:
The student will be able to identify common infectious agents and the diseases.
The student will be able to evaluate methods used to identify infectious agents in the
clinical microbiology lab.
The student will be able to recall microbial physiology including metabolism, regulation
and replication.
Course Outcomes
Evaluate standards for specimen collection, specimen integrity in the pre-analytical,
analytical and post-analytical delivery of patient care in microbiology.
Compare and contrast the general characteristics and the antigenic properties of gram
negative bacteria.
Summarize treatment strategies, expected antibiotic susceptibility results, and emerging
resistance for gram negative bacteria
Course Syllabus
Antibiotic and Antiviral resistant mechanisms. Basic knowledge of antibiotics and
antimicrobial therapy
Antibiotic and antiviral sensitivity test
DNA Transcription and translation Mutationand Mutgeresis
Recombinant DNA technology Genetics in Medicine.
Biomedical Waste Management
Diseases caused by Viruses- RNA Viruses
Diseases caused by viruses – DNA Viruses
Amoebiasis
Giardiasis techomoniasis , Bicol, Other free living amoeba
Malaria
Leishmaniasis
Toxoplasmosis
Pneumocystis, Crytopordiosis , Isospora
Taenia
Trematodes
Nematodes
Filariasis
Fungal infection: candida spp., Cryptococcus spp.;
Aspergillums spp, and Dermatophytes
Usual techniques, Immunological and molecular diagnosis of parasitic and fungal
diseases.
Innate and Adaptive immunity
Structure and functions of immune system
Antigens and Immunogens
Antibodies
Complements system
Antigen processing and presentation & Major Histocompatibility complex (MHC)
Cell-mediated immunity + Immune Response.
Hypersensitivity ,Autoimmunity
Immunology of transplantation malignancy + Immunodeficient diseases &
Immunohematology
Diseases caused by the bacteria ( Gram positive bacteria)
Anaerobes
Diseases caused by gram –negative bacilli
Mycobacteria
Spirochetes
Reference Books:
Biochemistry for Medical laboratory Technology by Lal H
Microbiology for Medical laboratory Technology by Arora D R
2nd year
COURSE –CLINICALBIOCHEMISTRY
Course Objective:
To teach students all theortical aspects of sample collection processing and reporting
To teach students indications for various biochemical tests
To teach students conventional and modern techquniqes of processing sample and obtain results
To train students to do tests using conventional techniques as well as semi automatic and
automatic machines
Course outcome
Students will hav knowledge of different aspects of sample collection processing and reporting
Students will know indications for various biochemical tests
Students will have enough knowledge of doing tests using conventional and modern techquniqes
of processing sample and obtain results
Students will be competant to do tests using conventional techniques as well as semi automatic
and automatic machines
Course Syllabus
LIVER AND BILIARY TRACT STATUS:
The dynamics and mechanisms of liver enzyme release and clinical utility of measuring
hepatic enzymes (e.g., aspartate aminotransferase, alanine aminotransferase, y-
glutamyltransferase, alkaline phosphates, and lactate dehydrogenate).
The biochemical assessment of liver function by nonenzyme analyses such as albumin,
ammonia, bile acids bilirubin, urea nitrogen, cholesterol, total protein, and triglycerides
RENAL FUNCTIONS
The basic physiology of renal function. The basic categories of renal diseases and be
familiar with the National Kidney Foundation practice guidelines for these condition.
The definition of osmalality molecules in serum that contributes to osmalality.,and
calculation of osmolal gap as well as the principle of the osmometer.The common pitfalls
and sources of error during estimation of the osmolal gap. The differential diagnosis of an
unexplained, increased osmolal gap, including alcohol or glycol ingestion alcoholic or
diabetic ketosis. The principles of fluid balance
GASTRIC & PANCREATE FUNCTION
The clinical manifestation of gastric, pancreate, and intestinal disease and diagnostic
methodologies such as the breath test for Helicobacter pylori, fecal occult blood, lipase
and amylase (e.g., fractionation of amylase; pancreatic VS salivary and amylase /
keratinize clearance ratio ).
The role of gastrointestinal hormones and enzymes in digestion and the evaluation of
malabsorption and diarrheal syndromes.
ASSESSMENT OF THYROID FUNCTION
The structure biosynthesis, secretion and metabolism of thyroid hormones (thyroxin
(T4), triodothyronine (T4) , and reverse T3 (rT3). Thyroid physiology and control of
thyroid function (thyrotrophic –releasing hormone (TRH) and thyrotrophic (TSH).
The common causes of hypothyroidism and hyperthyroidism
The laboratory tests for evaluation of thyroid disorders and be able to interpret these
analyses in their clinical context with an appreciation for the euthyriod sick state.
ACID – BASE CHEMISTRY WATER AND ELECTROYLTES BALANCE.
Define the Henderson – Hasselbach equation. Physiologic buffers systems and the role
of respiratory and renal compensation. Categories of clinical disorders of acid – base
balance (metabolic and respiratory acidosis, metabolic and respiratory alkalosis, mixed
disorders.)
The differential diagnosis of common electrolyte disorders
ISONENZYMES AND CLINICAL ENZYMOLOGY TUMOR BIOMARKERS :
The limitations of laboratory assessment of various tumor markers and the factors affecting the
results of different analytical procedures.
The conceptual basis of assays used to screen malignancy, including Byes theorem
AUTOMATION AND POINT OF CARE TESTING (POCT)
COLORI METER AND SPECTROPHOTO METRE
FLAME PHOTO METRE AND ION SELECTIVE
BLOOD GAS ANALYSIS
PH METER
ELECTROPHORESIS AND CHIROMATOGRAPHIC TECHNIQUES
THERAPEUTIC DRUG MONITORING AND TOXICOLOGY
COURSE-CLINICAL MICROBIOLOGY
Course Objective:
To teach students all theoretical aspects of sample collection processing and reporting
related to various microbiological tests like smear making, staining, culturing sample and
doing sensitivity testing
To teach students indications for various microbiological tests
To teach students conventional and modern techquniqes of processing sample and obtain
results
To teach students classification of micribials
To teach students life cycle and pathogenesis of different microbial organisms
To tech students method of diagnosis for individual organism
Course outcome
Students will have knowledge of different aspects of sample collection processing and reporting
related to various microbiological tests like smear making, staining, culturing sample and doing
sensitivity testing
Students will know indications for various microbial tests
Students will have enough knowledge of doing tests using conventional and modern techquniqes
of processing sample and obtain results
Students will know classification of microbial agents
Students will have knowledge of life cycle and pathogenesis of different microbial organisms
Student will have knowledge of diagnostic techniques for microbial infections
Course Syllabus
SYSTEMATIC BACTERIOLOGY
Normal flora of the human body.
Collection transport, processing of specimens of diagnosis of bacterial, viral and fungal
infection in the following cases. Respiratory tract infections.
Nosocomial infections: Epidemiology, bacterial and viral infections in paedriatic
patients, surveillance and control programmers,
Respiratory tract infections: Upper respiration tract: etiology, transmission,
pathogenesis
Urinary tract infections and sexually transmitted diseases –Bacterial ,viral and fungal
infections of the urinary tract,etiology,pathogenesis,transmission,clinical features
Gastrointestinal tract infections: etiology,pathogenesis,clinicalfeatures and diagnosis of diarrheal
diseases
Central nervous system infections :meningitis caused by bacteria viruses, fungi and protozoa,
viral encephalitis, brain abcesses,tetanus
Infections of the skin ,ear, and eye:Etiology,transmission diagnosis and prevention
Microbiology of air,water,and mild: common pathogens encountered ,methods for
microbiological analysis, methods for purification
Identification of Non-fomenters-Pseudomonas,Acinetobacter
Quick screening methods,Chromogenic agar media
Bacteriology and viral syndromes or diseases
PRACTICALS
Study of normal flora of human body
Isolation ,characterization and identification of pathogens form various clinical
specimens
Study of morphological, culture and biochemical characters of common bacterial
pathogens
Study of antibiotic sensitivity of common pathogens
Study of microbial flora of air in various localities
Microbial analysis of water
Microbial analysis of Milk
COURES-CLINICAL PATHOLOGY
Course Objective:
To teach students all theoretical aspects of sample collection processing and reporting related to
various pathological and cytological tests
To teach students indications for various pathological tests
To teach students conventional and modern techquniqes of processing sample and obtain results
To train students to do tests using conventional techniques as well as semi automatic and
automatic machines
To teach students indications of blood transfusion
To teach student process of blood collections and further process to separate components
Course outcome
Students will have knowledge of different aspects of sample collection processing and
reporting related to pathological tests
Students will know indications for various pathological tests
Students will have enough knowledge of doing tests using conventional and modern
techquniqes of processing sample and obtain results
Students will be competant to do tests using conventional techniques as well as semi
automatic and automatic machines
Students will know indications of blood transfusion
Student will have knowledge of process of blood collections and further process to
separate components
Course syllabus
General aspects: Blood cells formation, sites of haemopoiesis. Development of blood
cells. Morphology and regulation of haemopoiesis.
Red cells: Basic aspects of anemia definition,Patho physiology ,classification and
clinical features
Microcyctic hypo chromic anemia’s
Sideroblastic anemia
Anemia of chronic infection
Thalassaemia
Macrocyctic Anemia’s
Megaloblastic
Non megaloblastic
Megolblastic anemia
Normocyctic norm chronic anemia
Anemia in systemic disorder
Acute blood loss, Renal failure
Liver disorder etc
Disorder of Hemoglobin
Structure of Hb and synthesis
Normal and abnormal hemoglobin
Hamoglobinspathies
A plastic anemia
Polycythaemia
Leukocyte disorders
Leukaemoid reaction-type of leukaemoid and diagnosis
Leukaemias:Definition,French-American-British(FAB) and World Health
Organization-Classification of acute leukemia’s
Diagnosis criteria ,Cytochemical staining and Immunophenotyping
Myleproliferative disorder
Lymphroprolifeative disorder
Hemorrhagic disorder-Definition, Clinical features, Classification of primary
homeostasis, secondary homeostasis –causes and investigation of both.
Platelet disorders-Quantitative-Thrombocytopenia-Idiopathic,Classification,clinical
features
Thrombotic disorders : Classification, clinical features, Investigation of thrombotic
disorder
Blood Transfusion :
History of Transfusion Medicine
Blood groups and genetics
Blood transfusion n-Indications
Blood donation, Donor selection, blood collection
PRACTICALS
Staining and Interpretation of Peripheral
Microcyctic hypo chromic anemia
Microcyctic Anemia
Blood grouping
Reverse grouping
Grading of Reaction
Blood Collection
Reference Books:
Biochemistry for Medical laboratory Technology by Lal H
Microbiology for Medical laboratory Technology by Arora D R
Master of Science in Medical Laboratory Technology:2 YEARS FULL TIME DEGREE PROGRAMME
