Only ONE Change to Dramatically Improve
Hypersil GOLD®
METHODS
Thermo Scientific®
Core-Shell Technology
Thermo Scientific is a registered trademark of Thermo Fisher Scientific Inc. Hypersil GOLD is a registered trademark of Thermo Hypersil-Keystone Inc. Phenomenex is not affiliated with Thermo Scientific, Thermo Fisher Scientific, or Thermo Hypersil-Keystone.
The simple change
is…
0
0
100
200
mAU
20860
2 4 6 8 10 min
a
b c d*
0 2 4 6 8 10 12 14 min
a
b c d
0
100
mAU
20859
*
1
2
3
4
5
6 78
9
10
11
12
13
0
0
20
40
60
80
mAU
20886
2 4 6 8 10 min
Kinetex 5 µm core-shell columns provide considerable improvements for methods devel-oped on standard HPLC systems with conventional 3 µm or 5 µm fully porous columns - at low backpressures.
Thermo Scientific is a registered trademark of Thermo Fisher Scientific Inc. Hypersil GOLD is a registered trademark of Thermo Hypersil-Keystone Inc. Phenomenex is not affiliated with Thermo Scientific, Thermo Fisher Scientific, or Thermo Hypersil- Keystone. Comparative separations may not be representative of all applications
…Replace your 3 or 5 µm Thermo Scientific® Hypersil GOLD® Column with a
NEW Kinetex 5 µm Core-Shell HPLC Column
™
5 µm
Conditions for all columns:Columns: Kinetex 5 µm C18
Hypersil GOLD 5 µm C18Dimensions: 100 x 4.6 mm
Mobile Phase: A: Water with 0.1 % TFAB: Acetonitrile with 0.1 % TFA
Gradient: Time (min)0 10
% B5 95
Flow Rate: 1.2 mL/minTemperature: Ambient
Detection: UV @ 254 nmSample: Pharmaceutical Drug Mix
1. Procainamide 2. Acetaminophen 3. Folic acid 4. Sulfathiazole 5. Acebutolol 6. Dextromethorphan 7. Diphenhydramine 8. Propafenone 9. Amitriptyline 10. Fluoxetine 11. Naproxen 12. Diflunisal 13. Indomethacin
Better Chromatographic Performance• Higher Efficiency
• Improved Sensitivity
• Increased Resolution
Increased Resolution and Sensitivity by substituting fully porous column with Kinetex 5 µm
Higher Productivity by then reducing column length
Lower Costs by lastly adjusting gradient profile proportionally
0
0
20
40
60
80
100
120
mAU
20885
2 4 6 8 min
1
2
3
4
5
6 7
89
10
11
12
13
Phenomenex Kinetex® 5 µm C18
Thermo Scientific® Hypersil GOLD® 5 µm C18
90 Bar
90 Bar
Increased Resolution
Ap
p ID
208
85A
pp
ID 2
0886
a
b c d0
20
40
60
80
100
120
140
160mAU
20766
0 10 20 min
*0
20
40
60
80
100
120
140
160mAU
20767
0 10 20 min
a
b c d*
Conditons for all columns, except where notedColumn: Kinetex 5 µm C18
Fully porous 5 µm C18Dimensions: 250 x 4.6 mm
Mobile Phase: A: TFA/Water/THF (5/900/100) B: TFA/Acetonitrile/THF (5/900/100)
Gradient: Time (min) % B 0 10 40 80
Flow Rate: 1.5 mL/minTemperature: 40 °C
Detection: UV @ 295 nmSample: 1. Impurity a 4. Unidentified (*)
2. Impurity b 5. Paroxetine3. Impurity c 6. Impurity d
Ap
p ID
207
66
Ap
p ID
207
67
Kinetex® 5 µm XB-C18
Kinetex® 5 µm XB-C18
Fully Porous 5 µm C18
Kinetex® 5 µm XB-C18
Dimensions: 150 x 4.6 mm Dimensions: 150 x 4.6 mmGradient: Time (min) % B
0 10 24 80
Critical Advantages of Increased Resolution:
• Save time and money by adjusting method
• Separate critical pairs or difficult mixtures
• Less risk of failing system suitability requirements
Ap
p ID
208
59
Ap
p ID
208
60
• Shorter run time
• More runs per day• Less solvent used• Reduce disposal
costs
Drop-in a Kinetex 5 µm Columnand instantly attain . . .
Drop-in Kinetex 5 µm + Simple Method Adjustmentsto achieve...
OR
See Kinetex 5 µm on Your Method In less than 2 minutes, the Kinetex 5 µm Calculator computes the performance and cost benefits achievable by replacing your fully porous column.
http://www.phenomenex.com/methodmakeover
Phenomenex l WEB: www.phenomenex.com Phenomenex l WEB: www.phenomenex.com
Only ONE Change to Dramatically Improve
Hypersil GOLD®
METHODS
Thermo Scientific®
FL98
4711
12_W
Kinetex® 5 µm Columns (mm)
SecurityGuard™ ULTRA Cartridges*50 x 2.1 3/pk
Phases XB-C18 00B-4605-AN AJ0-8782C18 00B-4601-AN AJ0-8782PFP 00B-4602-AN AJ0-8787Phenyl-Hexyl 00B-4603-AN AJ0-8788
for 2.1 mm ID
SecurityGuard ULTRA Cartridges*50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 3/pk
Phases XB-C18 00B-4605-E0 00D-4605-E0 00F-4605-E0 00G-4605-E0 AJ0-8768C18 00B-4601-E0 00D-4601-E0 00F-4601-E0 00G-4601-E0 AJ0-8768PFP 00B-4602-E0 00D-4602-E0 00F-4602-E0 00G-4602-E0 AJ0-8773Phenyl-Hexyl 00B-4603-E0 00D-4603-E0 00F-4603-E0 00G-4603-E0 AJ0-8774* SecurityGuard ULTRA cartridges require holder, Part No. AJ0-9000. Kinetex 5 µm columns are also available in 21.2 mm ID.
for 4.6 mm ID
Terms and Conditions Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.
Trademarks Phenomenex and Kinetex are registered trademarks, SecurityGuard is a trademark of Phenomenex. Thermo Scientific is a registered trademark of Thermo Fisher Scientific Inc. Hypersil GOLD is a registered trademark of Thermo Hypersil-Keystone Inc.
Disclaimer Phenomenex is not affiliated with Thermo Scientific, Thermo Fisher Scientific, or Thermo Hypersil-Keystone. Comparative separations may not be representative of all applications.
© 2013 Phenomenex, Inc. All rights reserved.
If Kinetex core-shell columns do not pro-vide at least an equivalent separation as compared to a competing column of the same phase, return the column with the comparative data within 45 days for a FULL REFUND.
