Methods in pathologyMethods in pathology

AutopsyAutopsy:: An examination of a dead body was done by cutting it open to discover the cause of death.

Biopsy

Extracted from CIBA COLLECTION MEDICAL ILLUSTRATIONS

An examination of pathologic samples from living body to set up diagnosis

Cellular examination

Animal experimentation

Se-deficiency animal model

National Natural Science

Foundation of China (No. 30170832 )

Su Min, Zhang Rui-Juan et al.

Tissue and cell culture

One Newly Established esophageal carcinoma(EC) Cell Line CSEC-215 from EC patient in Chaoshan Region of China. Lu Shan-Ming, Su Min et al.

Neural stem cell National Natural Science Foundation of China (No. 30170832 ) Tian Dong-Ping, Su Min et al.

Immunohistochemistry and immuno-cytochemistry

Esophageal carcinoma

E-cad + in membrane Wu Ming-Yao et al.

GFAP+ in cytoplasm of neuroglia cell. Tian Dong-Ping, Su Min et al.

Fos+ in nucleus

Electron microscopic examination

Atypia of tumor cells(Pleomorphism of nuclei) in CSEC-215 generation 13 EM×3000

Confocal laser scanning microscopic examination

neuron

nuclear division DNA content

Confocal laser scanning microscopy

three-dimensional

In situ hybridizationIn situ hybridization

Chromosome analysis &

Gene clone

Biochip

基因芯片（引自陆祖宏教授课件）

组织芯片组织芯片（苏敏 李军提供）（苏敏 李军提供）