LaVision BioTec GmbHwww.lavisionbiotec.com

MI PLAN Series

Background

Two- and three-dimensional deep tissue imaging usually requires clearing while keeping the sample’s structure intact. Most of today’s clearing protocols involve agents and immersion media with specific optical characteristics (high RIs up to 1.57 and specific dispersions), which have to match those of the objective lens for optimum results. Additionally, deep tissue imaging requires large free working distances. Conventional water, glycerin or oil immersion lenses do not meet these requirements.

Multi-Immersion Planar Objective Lenses – Ideal for Cleared Samples

Key Parameters

NA Numerical Aperture

Determines how much light enters the objective; the higher, the better the

resolution

WD Working Distance

Distance between front lens element and focal plane; the depth in which a researcher

can image

RI Refractive Index

Determines how light propagates through the

immersion medium

Dispersion Change of RI with wavelength

Highlights

• Tailored to the optical characteristics (RI and dispersion) of a wide range of immersion media with RIs from 1.33 (water) to 1.57 (organic solvents)

• Correction of chromatic aberration

• Resistance against common media used for tissue clearing

• Long WDs for deep tissue imaging of large samples

• Correction of curvature of field (flat-field-correction) for images that are in common focus throughout the field of view

• Highly suitable for light sheet microscopy

LaVision BioTec’s Approach

For best results when it comes to deep tissue imaging of cleared samples, LaVision BioTec developed special apochromatic planar multi-immersion objective lenses.

WD

Curvature of Field

Flat-Field- Correction

Light Sheet

Objective Lens Magnification NA Dipping Cap RI Range of Imm. Medium Clearing Method WD

[mm]

1.1x NA 0.1 MI PLAN * 1.1x 0.1 DC40 WD16 AB 1.33 - 1.49Aqueous buffers, e.g.:

CLARITY, CUBIC, ScaleS 16

DC57 WD17 O 1.50 - 1.57Organic solvents, e.g.: BABB, DBE, DISCO, ECi 17

LVMI-Fluor 4x/0.3 WD6 4x 0.3 - 1.33 - 1.57Aqueous buffers, Organic solvents 5.6

12x NA 0.53 MI PLAN * 12x 0.53 DC33 WD8.5 W 1.33 - 1.41 Aqueous buffers 8.5

DC49 WD10.9 AB 1.42 - 1.48Aqueous buffers, e.g.:

CLARITY, CUBIC, ScaleS 10.9

DC57 WD10 O 1.49 - 1.57Organic solvents, e.g.: BABB, DBE, DISCO, ECi 10

1.1x NA 0.1 MI PLAN LVMI-Fluor 4x/0.3 WD6 12x NA 0.53 MI PLAN

MI PLAN Series – Specifications

* Each 1.1x and 12x MI PLAN objective lens is deli-vered with one specific dipping cap. The dipping caps are also available separately and can easily be exchanged.

Additional dipping caps not shown in this table are available by agreement.

Figure 1: The flat-field-correction of LaVision BioTec’s MI PLAN objective lenses guarantees a flat focal plane matching the optical axis of a light sheet.

Figure 2: Three-

dimensional image of a

mouse cortex demonstrating

the high isotropic

resolution of LaVision BioTec’s MI

PLAN objective lenses.

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LaVision BioTec GmbHAstastr. 1433617 Bielefeld, Germany

Tel: +49 (0) 521 915139-0 Fax: +49 (0) 521 915139-10info@lavisionbiotec.com

www.lavisionbiotec.com