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microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA...

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DNA microarray Pavlo Tertychnyi 6 March 2017
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Page 1: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

DNAmicroarrayPavlo Tertychnyi6March2017

Page 2: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Page 3: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Page 4: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

WhatisDNAmicroarray

Page 5: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

WhatisDNAmicroarray

Page 6: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Page 7: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Medicalfield

Helpresearcherslearnmoreaboutdiseases:1. Whatcausesdiseases2. Howtoidentifydiseases3. Howtotreatthem

Page 8: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Multiplesclerosis

Page 9: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Foodproduction

Page 10: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Foodproduction

Please,don’teatme…

Page 11: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Page 12: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Howdoesitwork

Page 13: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Howdoesitwork

Page 14: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Howdoesitwork

Page 15: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Example

Page 16: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Page 17: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Analyzingofresults

Page 18: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Analyzingofresults

Page 19: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Analyzingofresults

Page 20: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Analyzingofresults

Page 21: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

DNAmicroarraydatabases

Page 22: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Summary

1. Usedtoidentifyspecificgenesandtheactivityofgenes.2. Usedtodeterminehowourgenesareaffectedbyenvironmental

factors,drugsanddrugdosages.3. Wideusedinmedicinefield4. Identifyspecificcharacteristicsinhumanaswellasotherspecies.5. Foodproduction

Page 23: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Coolvisualization

https://www.youtube.com/watch?v=VNsThMNjKhM

Page 24: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Acknowledgement1. http://scme-

nm.org/index.php?option=com_docman&task=cat_view&gid=97&Itemid=225

2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011503/pdf/nihms-563981.pdf

3. http://www.ub.edu/stat/docencia/bioinformatica/microarrays/ADM/slides/A_Tutorial_Review_of_Microarray_data_Analysis_17-06-08.pdf

4.http://www.ebi.ac.uk/arrayexpress/5.https://www.ncbi.nlm.nih.gov/geo/6.http://www.hhmi.org/biointeractive/how-analyze-dna-microarray-data

Page 25: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Homework

1.WhatisDNAmicroarray?

a)Specificdatatypeb)Microchipc)SubsequenceofaDNAd)Giftofthegods

Page 26: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Homework

2.Describeatleast3applicationofDNAmicroarray(Imentionedtwoapplicationsduringthepresentation,souseyourimaginationorjustgoogletofindmore).3.DescribetheprocessofpreparationofamaterialbeforepouringitonaDNAmicroarray.

Page 27: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Homework

4.Jointhenextstatements:

a)Therearegenesofahealthycellmainly.b)Therearegenesofasickcellmainly.c)Therearegenesofbothhealthyandsickcells.

1)RedcolorofDNAmicroarrayunit.2)GreencolorofDNAmicroarrayunit.3)YellowcolorofDNAmicroarrayunit.

Page 28: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Sendyourhomeworksolutionshere

[email protected]

Page 29: microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

Thanks!


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