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MAS -100 ® Microbial Air Monitoring Systems MAS-100 ® MAS-100 EX ® MAS-100 Eco ® MAS-100 ISO ® MAS-100 CG Ex ® DA-100 ®
MAS -100®
Microbial Air Monitoring Systems
MAS-100®
MAS-100 EX®
MAS-100 Eco®
MAS-100 ISO®
MAS-100 CG Ex®
DA-100®
How the MAS-100® worksAll MAS-100® Microbial Air Monitoring Systems are sieve impaction systems based on the
Anderson impaction principle, accepted and proven world-wide.
Air is aspirated through a perforated lid with 400 holes. A radial fan,
controlled by a flow sensor, accurately regulates air flow real time at
100 liters/min. The air is impacted onto the surface of growth
media in a 90mm Petri dish or 60mm contact plate. Microorganisms
impact the media and the colonies are counted after the proper
incubation period. All MAS-100® microbial air samplers comply with
the guide lines as specified in ISO14698-1.
Sample collection accuracyThe MAS-100® Microbial Air Monitoring Systems provide the most accurate sample
collection available. The MAS-100® systems (except the
MAS-100 Eco®) utilize a state-of-the-art
mass flow sensor to assure a constant air
flow rate of 100 liters/min. The mass flow
sensor continuously regulates the air
flow rate during sample collection. This
allows the MAS-100® system to
automatically adjust for differences in
fill volume of Petri dishes, changes
in air density and differences between
individual perforated lids.
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The most complete and accurate line of microbial air monitoring systems available.
MAS-100®
The high-end,high precisionairsampler with long-term low operating costs
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MAS-100 Ex®
A special versionof MAS-100®
for use in explosion risk areas
MAS-100 Eco®
Inexpensive,reproducible,flexible and simple
MAS-100 ISO®
For microbiologicalair-monitoring in isolators andclean-rooms
MAS-100 CG Ex®
For controlling all types of compressed gases used in industry
DA-100®
CalibrationSystem:Allowing quickand easyrecalibrationon site
page 5 page 6 page 7 page 8 page 10
The MAS-100® product family
• Perfect in design – Precise in its measurements
• Unique – Built-in airflow compensation
• Independent – Use of standard petri dishes
Your advantages
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It´s so easy: just a short turn to open the perforated lid, and the standard petri dish can be removed for incubation.
Both the MAS-100® and MAS-100 Ex®portable microbial air samplers were
designed for use in cleanroom environments. These compact yetsophisticated devices are thepreferred choice for those demanding the highest qualityin microbial air monitoring. Both systems utilize either
90mm Petri dishes or 60mmcontact plates allowing for
a low operating cost and greaterflexibility. Sampling at 100 liters per
minute, these systems have the highestairflow accuracy available at ±2.5%,compared to others that can be as highas ±10%. The integrated flow sensorallows the user to freely interchange theperforated lids without affecting theaccuracy or the calibration of the unit.
Sampling volumes are also easily con-figurable between 0 and 2,000 liters. The units have a horizontal air flowvelocity of 0.45 m/sec. This isokineticflow rate coupled with our unique design will not produce turbulence in a laminar flow environment. The unitsare controlled using single touch "yes"or "no" responses. A programmablestart delay of up to 60 minutes allowsfor personnel to be out of the samplingarea when the sampling starts. TheMAS-100® microbial air sampler ispowered by a rechargeable NiMH batterypack which, when fully charged,provides for 7.5 hours of continuousoperation or about 50,000 liters of totalvolume.
The MAS-100® microbial air sampleralso features a bi-directional data portthat allows for the transfer of information to a PC via a specialcommunication cable. Sampling datacan be printed directly on a Seikothermal printer. Other features includeeasy on-site calibration using the
DA-100® Digital Anemometer system, a programmable calibration reminderprompt, easy to read liquid crystaldisplay, low battery alert and real timeclock with calendar.
MAS-100®Cat.No. 1.09090.0001
MAS-100® and MAS-100 Ex®
Height 26 cm
Diameter 11 cm
Weight 2.2 kg
Material Anodized aluminum
Diameter of Sampling Head 10 cm
Nominal Airflow 100 liters / min. + 2.5%
Standard Sampling Volumes 50, 100, 250, 500, 1000 liters
Freely Definable Sampling Volumes 1 to 2000 liters
Battery Pack NiMH rechargeable battery, 10 V (MAS-100®)
NiCad rechargeable battery, 10 V (MAS-100 Ex®)
Motor 6 V
Display Alphanumeric liquid crystal display, 32 characters
Lifetime RTC Battery RTC (Real Time Clock) battery; good for approx. 10 years
Driving Motor PWM frequency for driving motor
Processor Type 80C552
Airflow Regulation Hot-wire anemometer, numerical control
CE Approval EN 55022 Class B, EN 61000-4-2, ENV 50140,
ENV 50204, EN 61000-4-4, ENV 50141
Power Unit / Battery Charger 110-240 Volt, 50-60 KHz
Data Exchange RS232
Note: Specifications for the MAS-100 Ex® are the same as above except for the port for computer interface. This feature is not available on the MAS-100 Ex®.
Technical specifications
New contact plate holder 1.09214.0001 requires perforated lid 1.09213.0001.
Case comes standard
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The MAS-100 Ex® air sampler shares allof the features of the MAS-100® exceptfor the data port and NiMH battery.Because of its unique design, it is inaccordance with SN-SEV (NIN) makingit approved for use in Zone 2 areas. It is also approved for use with gasgroups IIA, IIB and IIC which constitutean explosion hazard in conjunctionwith flammable substances within thetemperature classes T1 to T4.to 4.
MAS-100 Ex®Cat.No. 1.09075.0001
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MAS-100 Eco®
Height (without handle) 14 cm
Diameter 11 cm
Height (with handle) 18 cm
Weight 1.4 kg
Material Anodized aluminum
Diameter of Sampling head 10 cm
Nominal Airflow 100 liters /min +4.0 %
Standard Sampling Volumes 10, 20, 50, 100, 200 & 500 liters
Freely Definable Sampling Volumes 0 -1,000 liters
Rechargeable Batteries 2 NiMH rechargeable batteries, 1.2 V
Motor 6 V
Display characters Alphanumeric liquid crystal display, 2 x 8
Lifetime RTC Battery RTC (Real Time Clock) battery; good for about 10 years
CE Approval EN 50081-1:1992 + EN 50082-1:1997
EN 50081-2:1993 + EN 50082-2:1995 + prEN 50082-2:1996
Power Unit / Battery Charger 110-240 Volt, 50-60 KHz
Output 5V DC/500mA
The MAS-100 Eco® microbial air sampleroffers an economical alternative to
our highly acclaimed MAS-100®microbial air sampler. This smaller,lightweight unit is ideal for appli-cations in or out of the clean-room and for air monitoring infood and beverage environments.The MAS-100 Eco® offers many
of the same features found on thestandard MAS-100® air sampler,
without the mass flow sensor. Like all MAS-100® systems, the MAS-
100 Eco® samples at a flow rate of 100liters per minute uses standard 90mmPetri dishes, providing a low operatingcost. The handle doubles as a stand to allow different angles for testing and an optional tripod adapter attaches to thehandle for fixed applications.
MAS-100 Eco®
Technical specifications
The perforated lids are the same as usedon the standard MAS-100® sampler. The simple user menu is easily accessedand controlled using single touch “yes"or “no" responses. Sampling volumes areprogrammable from 1-1,000 liters with 5 preset volumes to assure reproducibleresults. The unit is pre-calibrated but re-calibration on-site is easy with theaddition of our DA-100® Digital Anemo-meter.
Cat.No. 1.09227.0001
Technical specificationsCU, AU, VU SH
Nominal Airflow 100 Liters /min. +2.5%
Control Unit (CU)Dimensions (L/W/H) 175 x 235 x 120mm
Weight 3.7kg
Power 110/230 Volts
Display Alphanumeric liquid crystal display, 32 characters
Standard Sampling Volumes 100, 250, 500, 750, 1000 Liters
Material (Side Panels) Anodized aluminum
Anemometer Hot-wire anemometer, numeric control
Aspiration Unit (AU)Dimensions (L/W/H) 254 x 235 x 120mm
Weight 5.5kg
Power 110/230 Volts
Material (Side Panels) Anodized aluminum
Valve Rigid PVC/Viton
Valve Unit (VU)Dimensions (L/W/H) 175 x 235 x 120mm
Weight 4.5kg
Material (Side Panels) Anodized aluminum
Valves Rigid PVC/Viton/SS
Air Exit VU PVDF connector (12mm)
Consumption (Active) 0.25 A (58 V); AU + VU
Sampling Head (SH)Dimensions (H x Diameter) 860 x 110mm
Material (Perforated Lid) Anodized aluminum or stainless steel (L 316)
Material (Dust Cover) Anodized aluminum or stainless steel (L 316)
The new MAS-100 ISO® system isdesigned to meet the most rigorousdemands for efficient air monitoring ofthe microbiological cleanliness in iso-lators and cleanrooms.
The system consists of a control unit, valve unit, aspiration unit and asampling head. The sampling head(s)mount on standard 3/4” triclamp fittingswhich are themselves mounted throughthe walls of the isolator or cleanroom.Beside the sampling head there are noother electronic or mechanical compo-nents to be installed within the criticallycontrolled zone.
A special double-valve system ensuressecurity and also allows for integrationof the sampling head(s) into the steri-lization process of the isolator orcleanroom with no manipulation. Thesterilant, such as vaporized hydrogenperoxide (VHP), is circulated through the MAS-100 ISO® sampling head andvalve unit during the sterilizationprocess. The valve system protects theaspiration unit from damage by thesterilant. Each control unit can run 4
MAS-100 ISO®
sampling heads and in a BUS-system, upto 40 sampling heads at the same time.Calibration is easily performed on-sitewith the DA-100® Digital Anemometercalibration system.
Control Unit
Valve Unit Aspiration Unit
Sampling Head Ordering information see page 11
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MAS-100 CG Ex®
The MAS-100 CG Ex® system is for usewhen there is a need to test com-
pressed gases for microbial conta-mination. Utilizing two sensors,one to measure mass flow and the other for pressure, the unit collects samples under the pressure of the gas supply.
The MAS-100 CG Ex® systemsamples at two con-stant flow
rates, 100 L/min and 50 L/min forlow flow applications. The system will
sample gas at a pressure range between1.5 bar to 10 bar. Compressed gas is directed through a perforated plate ontothe 100mm Petri dish.
After sampling of the required volume of gas, a gradual decompression occursautomatically, preventing any suddenpressure change, avoiding possibledamage to the microorganisms and im-proving recovery. After decompression,the Petri dish is removed and incubated.
The MAS-100 CG Ex® system is the onlycompressed gas microbial air samplerapproved for use in Zone 2 explosionhazard areas. The MAS-100 CG Ex® is calibrated with compressed air. Due toour unique mass flow sensor technology,only a mathematical correction factor is required to sample other gasses. There is no need to calibrate for specificgasses. The unit is pre-programmed forcompressed air, nitrogen, carbon dioxide
and argon gas, and a total of 10 gasprotocols can be programmed andstored in the unit. The MAS-100 CG Ex®sampler makes compressed gas samplingeasy and accurate. The automatedcollection process saves time andeliminates the awkward manipulationsrequired with manual methods.
Cat.No. 1.09327.0001
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MAS-100 CG Ex®
Height 32.5 cm
Length 37.0 cm
Width 11.0 cm
Weight (without Sampling Head) 10 kg
Material Box Coated aluminum
Nominal Flow Rates 100 liter /min. ±5.0% (over the pressure
range) of 1.5 bar to 10 bar (absolute)
50 liter/min. ±5.0% over the pressure
range of 1.5 bar to10 bar (absolute)
Standard Sampling Volumes 50, 100, 250, 500, 1000 liter
Freely definable sampling volumes 1 to 2000 liter, volumes individually
selectable between 0 and 2000 liters.
0 volumes are not displayed
Pre-programmed gas types Air, nitrogen, carbon dioxide, argon
Battery Pack 20 cells NiMH, 3800mAh, voltage 24 V
Battery Charger 110-240 Volt, 50-60 Hz
Charger Input 36 V DC, 1.5 A
Display Alphanumeric liquid crystal display,
32 characters
Lifetime RTC Battery Approx. 10 years
Flow Valve Proportional, 24 Volt
Processor: Type: 80C552
Gas Regulation Mass flowmeter and pressure sensor
0-10 bar and proportional valve
CE Approval EN61000-6-1; 2001, EN61000-6-3; EN61000-6-2;
2001, EN61000-6-4; 2001; EN61326-1 + A1; 1998
Ex-Proof SNCH 02 ATEX 3418,
EN1127; 1997, EN 50021; 1999
Sampling Head
Head without Clamps, Height 16.0 cm
Diameter 10.0 cm
Weight 1.5 kg
Material Anodized aluminum, clamps of stainless steel
Autoclavable 20 minutes at 121°C
Tubing Length, 1.5m ID=10mm OD=19mm,
sterilize for 20 min at 121°C
Rapid Connectors Chromium-plated brass
Technical specifications
As with all our other MAS-100® air samplers,this unit uses the impaction method andstandard 90mm Petri dishes.
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All MAS-100® microbial air samplersare pre-calibrated, but on-site recali-bration is quick and easy with the addition of our DA-100® DigitalAnemometer. (Cannot be used on theMAS-100 CG Ex®.) The DA-100® is a highly accurate transfer standard. This digital vane anemometer spinsfreely on a magnetic field bearing(patent pending) and the revolutions are counted by a laser diode. These revolutions are then converted to volume flow rate and displayed onthe LCD on the unit.
The flow rate accuracy is ±1.0%, themost accurate on the market. The display also shows temperature (°C)and velocity in m/sec. The DA-100® Digital Anemometer uses a simple onebutton control. Calibration is accom-plished using the keypad on the MAS-100® air sampler, eliminating the needto open the MAS-100® to accesselectronics. The entire process takes lessthan 5 minutes. The DA-100® DigitalAnemometer is small, lightweight andcompletely portable. The DA-100®anemometer is pre-calibrated, certifi-cation is supplied.
When attached to the top of any MAS-100®unit (except the MAS-100 CG Ex®), theDA-100® digitalanemometer allows youto easily check bothvolume and air velocityon the spot... at any time.Recalibration can bedone on-site with noneed to open the units tomake adjustments! All calibrations are tra-ceable to NIST.
DA-100®Accuracy at 100 liters /min. + 1.0%Height 8.5 cmDiameter 11 cmWeight 0.9 kgVane Bearing Magnetic (patent pending)Material Anodized aluminumBattery Pack 9 V BatteryDisplay Alphanumeric LCD display, 2 x 8 charactersTemperature Display Resolution + 0.5°C incrementsAmbient Conditions Temperature 0 to 40°C,
humidity 0 to 80% (relative humidity)CE Tested EN 50081-1:1992 + EN 50082-1:1997
EN 50081-2:1993 + EN 50082-2:1995 + prEN 50082-2:1996
DA-100® Calibration System
Technical specifications
Cat.No. 1.09228.0001
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The instrumentsDescription Cat. No.MAS-100® Air Sampler 1.09090.0001
MAS-100 Eco® Air Sampler 1.09227.0001
MAS-100 Ex® Air Sampler, Explosion Proof 1.09075.0001
MAS-100 CG Ex® 1.09327.0001
MAS-100 ISO® Aluminum Sampling Head 1.09816.0001
MAS-100 ISO® Stainless Steel Sampling Head 1.09817.0001
MAS-100 ISO® Aspiration Unit 1.09813.0001
MAS-100 ISO® Control Unit 1.09812.0001
MAS-100 ISO® Valve Unit 1.09814.0001
DA-100® Digital Anemometer 1.09228.0001
Spare parts and accessoriesDescription Cat. No.MAS-100® Extra Dust Cover* 1.09084.0001
MAS-100® Mains Charger 1.09085.0001
MAS-100® NiMH Battery Pack 1.09229.0001
MAS-100® NiCd Battery Pack (For Version 2.62 or lower) 1.09087.0001
MAS-100® Extra Perforated Lid, Aluminum* 1.09088.0001
MAS-100® Extra Perforated Lid, Stainless Steel 1.09821.0001
MAS-100® Tripod 1.09326.0001
MAS-100® Tripod Adapter - Quick Connect 1.09223.0001
MAS-100® Tube Adapter 1.09224.0001
MAS-100® PC Interface Cable for Nova-LIMS® 1.09226.0001
MAS-100® Contact Plate Holder 1.09214.0001
MAS-100® Perforated Lid for Contact Plates 1.09213.0001
MAS-100 CG Ex® Extra Sampling Head, High Flow 100 L/min 1.09231.0001
MAS-100 CG Ex® Extra Sampling Head, Low Flow 50 L/min 1.09237.0001
MAS-100 Eco® Mains Charger 1.09128.0001
MAS-100 Eco® Tripod Adapter 1.09127.0001
MAS-100 Ex® NiCd Battery Pack 1.09087.0001
MAS-100 Ex® Extra Dust Cover* 1.09123.0001
MAS-100 Ex® Extra Perforated Lid, Aluminum* 1.09124.0001
MAS-100 ISO® Extra Dust Cover, Aluminum* 1.09823.0001
MAS-100 ISO® Software Package 1.09819.0001
MAS-100 ISO® Extra Perforated Lid, Stainless Steel 1.09821.0001
MAS-100 ISO® Extra Dust Cover, Stainless Steel 1.09822.0001
MAS-100 ISO® Pressure Test Kit 1.09129.0001
* Lids and Dust Covers are all interchangeable among MAS-100®, MAS-100 Eco®, MAS-100 Ex® and MAS-100 ISO®.
Ordering informations
Anderson, A. A. (1958) “New Sampler for the collection, sizing and enumeration of viable airborne particles." Journal of Bacteriology, Vol 76, pp. 471-484.
Meier, R., Zingre, H. (2000) “Qualification of air sampler systems: The MAS-100®" Swiss Pharma 22, No. 1-2, pp. 15-21
Feller, W. “An Introduction to Probability Theory and its applications", John Wiley and Sons Inc., New York, 1950
“Microbial Evaluation of Cleanroom Environments", U.S. Pharmocopiea 27 NF 23, General Chapters <1116>, 2004
References
MAS-100® Tripod
Merck KGaA64271 Darmstadt, GermanyFax: +49 (0) 6151/72 33 80Email: [email protected]
We provide information and advice to our customers to the best of our knowledge and ability, but without obligation or liability. Existing laws and regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose. DA100®, MAS-100®, MAS-100 CGEx®, MAS-100® Eco, MAS-100 Ex®, and MAS-100 ISO® are all trademarks of MBV AG, Switzerland.
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