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Antwerp Corrosion & Fouling Meeting 2019 01-4-2019 [email protected] 1 April 1, 2019, 2 nd international symposium on corrosion and fouling, Antwerp, Belgium Dr. Job Klijnstra +31 (0)6 10 49 00 59 Fouling control & material protection [email protected] Dr. Nanni Noël-Hermes +31 (0)6 46 84 72 96 MIC and biofilms [email protected] Microbial influenced corrosion (MIC) in maritime environment Corrosion, Failure analysis and Antifouling research since 1964 Former corrosion laboratory Royal Netherlands Navy Expertise Corrosion Electrochemistry Metallurgy Antifouling Microbiology Coating performance testing Material durability in seawater Natural seawater Outdoor exposure In-house laboratory of Royal Netherlands Navy a initiative ENDURANCE through RESEARCH
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Page 1: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

Antwerp Corrosion & Fouling Meeting 2019 01-4-2019

[email protected] 1

April 1, 2019, 2nd international symposium on corrosion and fouling, Antwerp, Belgium

Dr. Job Klijnstra +31 (0)6 10 49 00 59Fouling control & material protection [email protected]

Dr. Nanni Noël-Hermes +31 (0)6 46 84 72 96MIC and biofilms [email protected]

Microbial influenced corrosion (MIC) in maritime environment

Corrosion, Failure analysis and Antifouling research since 1964

Former corrosion laboratory Royal Netherlands Navy

Expertise

Corrosion

Electrochemistry

Metallurgy

Antifouling

Microbiology

Coating performance testing

Material durability in seawater

Natural seawater

Outdoor exposure

In-house laboratory of Royal Netherlands Navy

a initiative

ENDURANCE through RESEARCH

Page 2: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

Antwerp Corrosion & Fouling Meeting 2019 01-4-2019

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Microbial influenced corrosion

MIC is a rapid form of corrosion initiated or accelerated by microorganisms

Localized form of corrosion

Direct or indirect mechanism

Up to 20% of all serious corrosion events are related to MIC

It affects a wide diversity of industries such as maritime & offshore, water distribution and waste water treatment systems, oil and gas, food industry

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General facts about microorganisms:

Characteristics of microorganisms related to MIC: (Borenstein, 1994)

Page 3: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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Microorganisms (MO’s) interact with surfaces and form a biofilm:

(a) = cells attach to the surface biofilm formation

(b) = aerobic and anaerobic zones are formed MO’s create their own (micro) environment

(c) = ions are trapped, localized chemical and physical gradients are created at the metal surface dissolving of the metal and pit formation

Biofilms, critical factor for MIC

Preferred sites for attachment are (micro-) scratches, cracks, crevices, etc.

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MIC - complex processes

Uptake of nutrients out of liquid or soil, conversion to

acids and other corrosive by-products such as CH4, H2S,

etc.

Change of local environmental conditions

acceleration of corrosion processes

resulting in pitting, cracking and other forms of corrosion damage

Damage of materials such as metals, concrete or polymers is possible!

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Page 4: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

Antwerp Corrosion & Fouling Meeting 2019 01-4-2019

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MIC in maritime environment

Types of corrosion

Severe corrosion

splash zone

Tidal zone

Accelerated low water

corrosion (ALWC)

Localized corrosion

under clusters of

macro fouling

Localized corrosion

in sediment

Seaweed

Mussels

Tunicates,

Hydroids,

Sponges

Clusters

of mussels

Types of fouling

HWL

LWL

! ALWC !

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MIC in maritime environment

Page 5: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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Diversity in corrosion related microorganisms

Not only SRB are relevant for MIC!

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Table 2.1 Brock Biology of Microorganisms 11/e, © Pearson Prentice Hall, Inc.

Diversity of microorganisms

Page 6: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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Situation: mooring piles coated only above low water

level (LW); uncoated below LW and in soil

Perforations after 5 years just below LW-level;

Some piles broken within 5 years

Corroded locations characterized by:

- Very severe local attack from outside

- Pits were bowl shaped, covered by red-orange

colored corrosion products

- Underneath blank shiny steel

- No fouling on corroded spots

Mooring pile in a small salt water harbour

MIC in maritime environment

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HT-LT zone

Aerobes SRB

SOB

MIC in maritime environment

Page 7: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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Anaerobic conditions under a biofilm

Within the biofilm corrosion by Sulfate Reducing Bacteria (SRB)

Pitting corrosion with iron sulfide as corrosion product.

Biofilm is frequently disturbed by mooring ships and tidal movements

Oxygen access to metal surface aerobic conditions

Sulfur compounds are oxidized by aerobic bacteria to sulfuric acid, leading

to serious local attack and providing substrate for SRB

Mooring pile case

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MIC in maritime environment

Coating samples were exposed to suspensions with different strains of bacteria:

Sulfur oxidizing and acid producing bacterium, Acidothiobacillus thiooxidans

Slime forming bacterium that creates local chemical gradients, Pseudomonas fluorescens

Sulphur reducing and very corrosive bacterium Desulfovibrio indonesiensis

Mixed bacterial biofilm collected from a coated ballast tank

Exposure experiments were conducted at 28 ̊C for a period of 60 days;

Barrier properties of coatings were determined by EIS measurements

Microbial attack of protective coating

Objective: are tank coatings sensitive to microbial degradation?

A solvent free pure epoxy ballast water tank coating was investigated in two ways:

Applied onto stainless steel panels for EIS measurements

As a free film to observe coating degradation

Page 8: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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MIC in maritime environment

Barrier properties of BWT coating exposed to bacteria

S. Indonesiensis

Conclusion: Specific bacteria can have strong negative effect on protective properties of ballast tank coatings

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MIC in maritime environment

Microbial attack of protective coatings

Exposure of free film epoxy coating to solutions with the mixed bacterial biofilm

showed striking results.

a) Flask with isolated bacteria without

medium and coating material: no growth!

b) Flask with coating flakes without bacteria:

no degradation!

c) Flask with isolated bacteria without

medium but with coating flakes: growth!

d) Flask with bacteria in their medium and

with coating flakes: growth!

Conclusion: Ballast tank coatings can be sensitive to biodegradation

Page 9: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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MIC in maritime environment

After 1 year

Analysis showed presence and activity of MIC relevant MO’s

Tubercles of corrosion products; underneath corrosion pits

Coupons at the inside:- close to air/water interphase, corrosion rate of 0.14 mm/year - close to the sediment, corrosion rate of 0.05 - 0.1 mm/ year

Mini monopile test setup

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water-sediment interphase

air-water interphase

submerged

Mini monopile after 7 years

MIC in maritime environment

Page 10: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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Simple test set-up: exposure of carbon steel coupons in flasks with natural seawater and sand /sediment samples

MIC at water/ sediment interphase

Seawater

Sediment

carbon steel: 6 months exposure

Laboratory tests for offshore wind

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Seawater

Sediment

carbon steel: 6 months exposure

Attacked surface area with maximum pith depth around 240 µm

Average pit depth: 34 µm

Laboratory tests for offshore wind

Page 11: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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MIC Diagnosis

Case by case approach

Combine different knowledge fields and experimental techniques

Mere presence of microorganisms is not enough !

Are MO’s active and is there a relationship with the damage pattern?

Are necessary nutrients available?

MIC yes or no ?

... MIC diagnosis is like a puzzle ...

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MIC diagnosis and failure analysis

Case documentation

Microbial analysis (e.g. growth- &

DNA based)

Microbial related failure analysis

Detect or exclude other corrosion

mechanisms

ATP tests

Page 12: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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Detect or exclude other corrosion mechanisms

Other corrosion mechanisms are often not taken into account: Failure in the choice of a suitable mitigation strategy MO’s are found present but their activity is not established or investigated Often MIC is “blamed” when other explanations cannot be given

“Chicken or egg” question: what was first? Wrong choice/ failure of material/ no protection of material

or Presence and activity of microorganisms

MIC may take place regularly in conjunction with other corrosion mechanisms:

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Galvanic corrosion

Stray current

Under deposit corrosion

MIC diagnosis and failure analysis

1 Coatings: Correct choice of coating (part of structure, exposure conditions, lifetime, …) Proper application (surface pre-treatment and condition, equipment, ...) Difficult for mudline or area under seabed

Metallization (TSZ, TSA) seems to be promising but further investigations required, for instance in offshore wind

2. Cathodic protection: Depending on potential applied and maybe also on MO’s present Install and operate CP right from the beginning! Late installment and

interruptions may give problems

CP will probably work but optimal settings and conditions are still under investigation Cases are described in which biofilm formation was stimulated when using CP

3. Other mitigation strategies Biocides, corrosion inhibitors UV light, ultrasound

Chemicals often not possible in open systems; physical methods still under investigation

Prevention and mitigation of MIC

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Page 13: Microbial influenced corrosion (MIC) in maritime environmentcorrosion.hzs.be/Presentations/Klijnstra.pdfAntwerp Corrosion & Fouling Meeting 2019 01-4-2019 Nanni.Noel@Endures.nl 1 April

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Take home messages

MIC needs to be analyzed on a case by case basis

Other corrosion mechanisms need to be established or ruled out

Investigate not only the presence but also the activity of MIC relevant MO’s

Numbers of MO’s from test kit results are useless unless a clear relationship with the (damaged) metal is established

There is not one “powerful tool” against MIC, it is a combination of multiple factors

Activity of corrosion related MO’s should be monitored/ controlled for proper protection of maritime structures against MIC

Think about a prevention strategy before the structure is installed

Thank you for your attentiona initiative

Questions ?

[email protected]


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