Page 1
Microbiology Practical I
The following slides were contributed by your fellow BIO 251 students…
Use side “” key to view slides…
Page 2
Lab 1: Eukaryotes and Microscopy
Know the parts of the microscope…
Page 3
Lab 1: Eukaryotes and MicroscopyParamecium caudatum
Page 4
Lab 1: Eukaryotes and MicroscopyEuglena gracilis
Page 5
Lab 1: Eukaryotes and MicroscopyAmoeba proteus
Page 6
Lab 1: Eukaryotes and MicroscopyFungus – Saccharomyces cerevisiae
Page 7
Lab 1: Eukaryotes and MicroscopyCyanobacteria ~ Nostoc
Page 8
~Bacteria slides~
Micrococcus luteus
Page 9
~Bacteria Slides~
Bacillus subtilis
Page 10
~Bacteria Slides~
Bacillus anthracis
Page 11
~Bacteria Slides~
Rhodospirillum rubrumSorry not in focus…
Page 12
~Bacteria Slides~
See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining…
[ Adobe Acrobat of Gram and Endospore ]
Some Endospore Staining ~
Page 13
Streak Plate:
Know colony characteristics (color, elevation, margin, etc.)
Page 14
Spread Plate vs. Streak Plate:
Page 16
Spread Plate:Mixed – Bacillus subtilis & Serratia marcescens
Page 17
Practical I Unknown Tests:
~ Simple Stain~ Gram Stain~ Endospore Stain~ Acid-Fast Stain (Demo)~ Capsule Stain (Demo)~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep)
~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles (Gas Pak, Eugon Agar, Thioglycollate Broth)
Page 18
Gas Pak Anaerobic vs. Aerobic:
Page 19
Dilution Problems:
Page 20
Dilution Problems:
Page 21
Bacterial Growth Curve:Make sure you know how to:
Use a spectrophotometer to measure absorbance.
Produce a log graph of the calculations.
Calculate generation time from a growth curve.