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Microscope Couplers

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    6 5 4 0 B U R R O U G H S .

    S TE R L I N G H E I G H TS

    M I C H I G A N . U S A . 4 8 3 1 4 2 1 3 3

    P H O N E 5 8 6 . 7 3 1 . 6 0 0 0 F A X 5 8 6 . 7 3 1 . 6 4 6 9

    E M A I L i n f o @d i a g i n c . c o m

    WE B S I TE w w w .d i a g i n c . c o m

    For your general digital cameraneeds, request our

    SPOT Insight Brochure.

    For your substage illuminatorneeds, request our

    Transilluminating Bases Catalog.

    For your boom stand needs,request our Stereo Microscope

    Boom Stands Catalog.

    MICROSCOPE COUPLERSF O R V I D E O , D I G I TA L & 35mm C A M E R A S

    For your low light digital cameraneeds, request our

    SPOT RT Brochure.

    inst rument s, inc.

    inst rument s, inc.

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    COUPLER SERIES SELECTION GUIDE

    2

    How To Choose:

    Select your cameras image capture technology

    Select video or high resolution digital

    Information You Will Need:

    Camera information:

    1CCD, 3CCD or 35mm filmCCD sizeAnalog or DigitalCamera lens mount

    Microscope information:

    Brand, ModelAttachments

    Select key features for your application

    Go to page numbers indicated for more detail

    Appendices1, 2& 3

    Pages 34-39

    Reference Appendices:

    Field of View Diagrams M agnification Equations

    Object Size Equations Parfocalization Instructions

    1CCD

    35mm FILM

    ANALOG

    (Video)

    HR & HRP

    DIPS

    HRD & HRU

    PA1

    PAGES14-17 & 20-2

    PAGES18-21

    PAGES30-33

    PAGES8-13

    DIGITAL

    3CCDIMAGE

    CAPTURED BY:ANALOG (Video)

    & DIGITALT45 & T60 PAGES

    22-29

    1.0

    xO

    NLY

    Key Features:

    High performance parfocal lens design at an economical price

    Positions camera closer to microscope

    Available in C-Mount and no-lens 1/2 ENG & Nikon F mounts

    Designed for removable phototube microscopes

    Key Features:

    High performance lens system for high resolution digital cameras

    Multi-coated lenses & twist style focus

    Available in C and Nikon F mounts

    Interchangeable bottom clam ps for nearly all microscope models allows use of one coupler tosupport multiple microscopes

    Key Features: Designed specifically to eliminate color shading in three chip camera designs

    Multi-coated lenses & twist style focus

    Available in C, Sony 1/2 ENG and non-Sony 1/2 ENG lens mounts

    Interchangeable bottom clam ps for nearly all microscope models allows use of one coupler tosupport multiple microscopes

    Key Features:

    Inexpensive and easy to use parfocal designs

    Most popular microscope models accommodated

    Interchangeable T-mount adapters allow most any 35mm camera to be mounted

    Used for applications requiring greater than 1.0x magnification

    CDC

    Key Features:

    Coated lenses & twist style focus Low to moderate price

    Available in C and Nikon F mounts

    Interchangeable bottom clam ps for nearly all microscope models allows use of one coupler tosupport multiple microscopes

    Key Features:

    High performance parfocal lens design at an economical price

    Designed for consumer digital cameras

    Largest selection of microscopes accomodating old and newPAGES

    4- 7

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    Selected Consumer Digital Camera Couplers for Various Mi croscopes

    Benefits of the Consumer Digital Camera System

    High Quality

    Produces crisp, flat field images with all

    microscope objectives including apochromats,

    plan fluorites, and achromats.

    Economical

    Priced up to 50% lower tha n majormanufacturers couplers.

    Compact Design

    Positions camera close to microscope for great er

    stability, convenience a nd a esthetics.

    Parfocal

    Precise machining provides parfocality

    between the microscope and the camera.

    Wide Field of View

    Use cameras zoom feature to set your

    field of view.

    Microscope Mounts

    The largest selection a vailable.Camera Mounts

    28mm x .75 pitch filter thread mount.

    37mm x .75 pitch filter thread mount.*

    43mm x .75 pitch filter thread mount.**Certain cameras may exhibit vignetting of image.

    Note: Older microscopes that use compensating optics

    may produce images with lateral chromatic abberat ion

    and spherical abberations uncorrected (see Appendix 4).

    Nikon Cool Pix Series Camera on a Nikon E200 Microscope

    The System . . .

    Camera Lens Mount

    Choose f rom 28mm , 37mm * and 43mm *filter thread mounts.

    Mi croscope Mount

    CDC Couplers accommodate nearly any microscope model.

    Couplers are ordered a s one catalog number specific for your

    microscope (see selection guide on pages 6-7).

    Field of View

    The cameras zoom system allows you to set y our field of

    view close in for detailed image capture or zoom wa y out

    for overa ll morphology.

    CDCXXNLFor ISO 38mm Phototubes

    (Nikon & Leica)

    CDCXX23

    For 23.2mm Phototubes

    CDCXX30For 30mm Phototubes

    Selected Consumer Digital Camera Couplers for Various Mi croscopes

    CDCXXHC

    For Leica HC Tube

    CDCXXBX

    For Olympus BX/AXCDCXXBB

    For Bausch & Lomb Stereozoom 6and Balplan Microzoom

    CDCXXAD

    For American Optical Diastar,

    Microstar IV

    CDCXXZNFor Zeiss Axio-2 Se

    Computer Optimized Lens System

    CD C C oupler systems are computer optimized and expertly

    manufactured. All lenses are multicoated optical glass to

    ensure crisp, flat field images on your camera.

    CD C Series for Consumer D ital Cameras with Filter Thread Mounts

    CONSUMER DIGITAL CAMERA COUPLERS /CDC SERIES

    4

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    D irect Image P rojection for C -Mount & B ayonet Mount C ameras

    1CCD CAMERA COUPLERS /DIP SERIES

    Selected Direct Im age Projection Couplers for C-Mount Camera s

    Benefits of the Direct Image Projection System

    High Quality

    Produces crisp, flat field images with all

    microscope objectives including apochromats,

    plan fluorites, and achromats.

    Economical

    Price range 13%-250% lower than majormanufacturers couplers.

    Low ProfilePositions camera CCD chip at the intermediate

    image plane, closer to microscope for greater

    stability, convenience, and aesthetics.

    Parfocal

    Precise machining provides parfocality

    between t he microscope and the TV monitor

    (see Appendix 4 on Parfocalizing) .

    Wide Field of View

    0.50x (for 1/2" CC D ) and 0.63x (for 2/3" CC D )

    provide wider field of view on monitor.Camera Mounts

    C-Mount, Nikon F-Mount, Sony 1/2" ENG Mount

    and Non-Sony 1/2" ENG Mount.

    Note: Older microscopes that use compensating optics

    may produce images with lateral chromatic abbera tion

    and spherical abberations uncorrected (see Appendix 4). 0.63x (D63BXC) to attach 2/3" CCD camera to Olympus BX

    The System . . .

    Camera Lens MountChoose from the large selection of C-mount and

    F-mount models.

    No Bottom Clamp

    The D irect Image Pro jection couplers are ordered as one

    part, specific for a range of microscopes. They do not

    utilize B ottom Clamps as the HR video coupler series does.

    Direct Ima ge Projection Concept

    The camera C CD chip is positioned where the microscope

    intermediate image is located. This provides a low profile,

    stable and more compact system. The system is parfocal

    with proper microscope set-up. See Appendix 4 about

    Parfocalizing a microscope.

    8

    D10BXC, D63BXC, D50BXC

    For Olympus BX, AX & MX SeriesD10HCC, D63HCC, D50HCC

    For Leica DMR, DMRD, DMLS, DMLB

    D10NLC, D63NLC, D50NLCFor Nikon & Leica ISO 38mm Ports

    Selected Direct Im age Projection Couplers for Nikon F-Mount Cameras

    D10ZNC, D63ZNC, D50ZNC

    For Zeiss Axiolab, Axioplan-2,Axiophot-2, Axiotech & New Standard

    D10HCFFor Leica DMR, DMRD, DMLS, DMLB

    D10NEF

    For Nikon Eclipse, E400, E600 Series

    D10E8FFor Nikon Eclipse, E800, E100 Series

    D10BXF

    For Olympus BX, AX & MX SeriesD10ZNF

    For Zeiss Axiolab, AxioAxiophot-2, Axiotech & New

    Direct Image Projection Lens System

    For a wider f ield of view, a 0.50x (for 1/2" CC D ) and 0.63x

    (for 2/3" CC D ) reduction lenses are ava ilable for all C-mount

    versions of the Direct Ima ge Projection series. No -lens

    versions are also available which provide a 1.0x magnification.

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    1CCD CAMERA COUPLERS /DIP SERIES

    10

    How To Choose:

    Select your cameras lens mount

    Select your cameras CCD size

    Select field of view (to visualize the different field of view sizesachieved with each magnification, see Appendix 2 on pages 36-37)

    Go to page numbers indicated (once a magnification is selected,the specific catalog number for your microscope can be found inthe chart on pages 12-13)

    Identifythe CCD

    format size

    1/2"4 .8mm x 6 .4mm

    Kodak KAF0402M E

    Sony ICX205

    1"9 .6mm x 12.8mm

    Kodak KAI2000

    Kodak KAF3200E

    Widest field of view

    Smaller, magnifiedfield of view

    0.5x

    0.63x1.0x (no lens)

    Widest field of view

    Smaller, magnifiedfield of view

    Widest field of view

    Smaller, magnifiedfield of view

    0.5x

    0.63x1.0x (no lens)

    0.63x

    1.0x (no lens)

    2/3"6 .6mm x 8 .8mm

    Kodak KAF1402M ESony ICX285

    Camera

    Lens Mount

    C-Mount

    Nikon F

    Bayonet Mount

    Sony 1/2"ENG Bayonet

    1/3"3 .6 m m x 4 .8 m m

    Coupler Magnification

    Non-Sony 1/2"ENG Bayonet

    SPOTEnhanced

    SP500

    SPOT InsightSPOT RT-Mono

    SPOTEnhanced

    SP600

    1.0x (no lens)

    1.0x (no lens)

    Choose desiredfield of view

    (see Appendix 2 forfield of view diagrams)

    Identify thmicroscop

    pages 12-1order the c

    number unddesired mag

    tion column.

    For widest field of viewsee T45 & T60pages 22-29

    For widest field of viewsee T45 & T60pages 22-29

    Selection G uide

    SPOT RT-ColorSPOT RT-Slider

    SPOT EnhancedSP640

    For widest field of viewsee HRD & HRU

    pages 18-211.0x (no lens)

    1"9 .6mm x 12 .8mm

    Kodak KAI2000Kodak KAF3200E

    2/3"6 .6mm x 8 .8mm

    Kodak KAF1402M E

    1/2"4 .8mm x 6 .4mm

    Kodak KAF0402M E

    1.0x (no lens)Widest field of view

    SPOT EnhancedSP540

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    Monitor vs. E yepiece

    APPENDIX 2 / FIELD OF VIEW

    Field of View Diagram

    In ea ch diagram, the circles represent

    the field of view seen through the micro-

    scope eyepieces. The rectangles represent

    the image that will appear on the TV

    monitor (or computer screen).

    Ea ch circle corresponds to a certain

    field number (FN) eyepiece. Eyepieces

    with wider fields have larger field

    numbers. The field number is usually

    engraved on the e yepiece following the

    magnif icatio n, as in WFK 10x/20.

    In this example, FN= 20.

    Choosing a Magnification

    U sually the d esired field of view on

    the monitor is that which most closely

    corresponds to wha t is seen through the

    microscope eyepiece. This is achieved

    by selecting the lowest magnification

    coupler recommended for a specific chip

    size.

    However, there are situations that

    require a higher magnification coupler.

    Specimens that a re blurred on the

    monitor are better resolved at a higher

    magnification by utilizing more pixels on

    the monitor. This is especially useful

    when using a 100x objective which is

    usually the highest a vailable microscope

    magnification. A higher magnification

    coupler may also be useful when using a

    non-flat field o bjective. This enables the

    36

    1CCD C-Mount 1CCD C-Mount 3CCD B ayonet or C-MountD irect Image Projection Couplers HR /HR P Couplers T45 &T60 Couplers

    1CCD C-Mount 1CCD C-Mount 3CCD B ayonet or C-MountD irect Image Projection Couplers HR /HRP Couplers T45 &T60 Couplers

    1/3" CCD Cameras

    1/2" CCD Cameras

    1CCD C-Mount 1CCD C-Mount 3CCD B ayonet MountD irect Image Projection Couplers HR /HR P Couplers HRP100-ENG 23 Coupl

    Kodak KAF-0402ME Kodak KAF-1402ME Kodak KAF-3200ESP OT E nha nced -SP 500 & SP 540 SP OT E nha nced -SP 600 & SP 640

    Kodak KAI-2000/2001 Sony SonySPOT Insight, SPOT RT ICX205 ICX061, ICX085, IC

    2/3" CCD Cameras

    Digit al Cameras

    user to image only the center (in focus)

    portion of the image.

    Ultrawide vs. Standard Field of View

    What makes an HRU060 ultrawide

    field coupler different from an HRD060

    standard field coupler? It is the size of

    the image the bottom lens captures. The

    ultrawide design captures a 26mm

    diameter image, whereas a standard

    coupler captures a 19mm diameterimage. Capture and correction of this

    larger field of view req uires larger and

    more exotic (apochromat ic glass) lenses

    making the HR U 060 ultrawide coupler

    correspondingly more expensive.

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