Microscopes

Rules for using a microscope1. Always carry the microscope with 2 hands2. Always start with the lowest power 3. Return to the lowest power when

Changing slides lost object Putting away

4. Only use fine adjustment knob with the high power

5. When putting away Wrap cord loosely around base Stage at the bottom

Ocular

Body Tube

Revolving Nosepiece

Objectives

Stage

Diaphragm

Coarse Focus

Fine Focus

Arm

Stage Clamp

Light

Base

•The area of the object that you actually see when looking through the microscope

Field of view (diameter of view)

Object 4X 10x 40x

Depth of view– how deep into the drop or

specimen you can see

4xslide

Cover slipspecimen

Objective lens

10x 40x

• how much space between the objective and the slide

4x

Working distance

Stage

Working distance Working

distance Working distance

10x 40x

Illumination

• The amount of light that is able to pass through the objective

4x

It gets dimmer

10x 40x

Calculating magnification

• Multiply the ocular magnification by the objective magnification of the power you are using

• Ex: Ocular 5x and objective is 10x

• 5 X 10 = 50x total magnification

Discovery of cells

•Anton van Leeuwenhoek–Invented the first microscope – 1600’s

–Viewed cork cells (dead wood)

–Named them “cells”

Robert Hooke

Matthias Schleiden

• 1830’s observed plant cells• All plants are composed of cells

Theodore Schwann

• Views animal cells• Concluded all animals are made of

cells

Cell Theory1. All living things are made

up of cells2. All cells come from

preexisting cells3. Cells are basic units of

structure and function of life