NInstitute Report No. 293

0

Mutagenic Potential of DIGL-RP Solid PropellantIin the Ames SalmonellalMammalian

Microsome Mutagenicity Test

Steven K. Sano, BA, SGTand

Don W. Korte, Jr., PhD, MAJ, MSC

GENETIC TOXICOLOGY BRANCH

DIVISION OF TOXICOLOGY

DEC 1 9 1988D

September 1988 Toxicology Series: 150

LETTERMAN ARMY INSTITUTE OF RESEARCHPRESIDIO OF SAN FRANCISCO. CALIFORNIA 94129

V.. fisoas .u m.| un |

Mutagenic Potential of DIGL-RP Solid Propellant in the Ames Salmonella/MammalianMicrosome Mutagenicity Test (Toxicology Series 150)-.Sano and Korte

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Institute Report No. 293

6a. NAME OF PERFORMING ORGANIZATION 6b. OFFICE SYMBOL 7a. NAME OF MONITORING ORGANIZATIONGenetic Toxicology Branch (If applicable) US Army Biomedical Research and DevelopmentDivision of Toxicology SGRD-ULE-T Laboratory

6c. ADDRESS (City, State, and ZIP Code) 7b. ADDRESS (City, State, and ZIP Code)Letterman Army Institute of Research Ft. DetrickPresidio of San Francisco, CA 94129-6800 Frederick, MD 21701-5010

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62720A 835 AB DA 30391311. TITLE (Include Security Clasification) Mutagenic Potential of DIGL-RP Solid Propellant in the

Ames Salmonella/Mammalian Microsome Mutagenicity Test

12. PERSONAL AUTHOR(S)Steven K. Sano and Don W. Korte, Jr.

13a. TYPE OF REPORT 113b. TIME COVERED 14. DATE OF REPORT (Year, Monthi,Day) 1 S. PAGE COUNTInstitute FROM8/19/85 TOIL94/8 1988 September 17

16. SUPPLEMENTARY NOTATIONToxicology Series 150

17. COSATI CODES 'I. SUBJECT TERMS (Continue on reverse if necesary and identify by block number)

FIELD GROUP SUB-GROUP ) Mutagenicity, DIGL-RP,Genetic Toxicology, Solid Propellant. )-

I I"Am s T e s t-

19. ABSTRACT (Continue on reverse if necesnary and identify by block number)

The mutagenic potential of DIGL-RP solid propellant was assessed by using the AmesSalmonella/Mammalian Microsome Mutagenicity Tet - Tester strains TA97, TA98, TA100, andTMi02 were exposed to doses ranging from 5 mg/plate to 0.0016 mg/plate. The test compoundwas not mutagenic under conditions of this test. --

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ABSTRACT

The mutagenic potential of DIGL-RP solid propellant wasassessed by using the Ames Salmonella/Mammalian MicrosomeMutagenicity Test. Tester strains TA97, TA98, TAI00, andTA102 were exposed to doses ranging from 5 mg/plate to0.0016 mg/plate. The test compound was not mutagenic underconditions of this test.

Key Words: Mutagenicity, Genetic Toxicology, Ames Test,DIGL-RP, DEGDN, Solid Propellant

Accession For

j;IJIS GRA&IIIIC TABUnannouncedJuLtificatio

ByDistribution/NE

i Availability Codesi'Aanrador

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• m m | l | |i

PREFACE

TYPE REPORT: Ames Test GLP Study Report

TESTING FACILITY:

US Army Medical Research and Development CommandLetterman Army Institute of ResearchPresidio of San Francisco, CA 94129-6800

SPONSOR:

US Army Medical Research and Development CommandUS Army Biomedical Research and Development LaboratoryFort Detrick, Frederick, MD 21701-5012Project Officer: Gunda Reddy, PhD

PROJECT/WORK UNIT/APC: #3EI62720A835/180/TLBO

GLP STUDY NUMBER: 85014

STUDY DIRECTOR: MAJ Don W. Korte Jr., PhD, MSC

PRINCIPAL INVESTIGATOR: SGT Steven K. Sano, BA

REPORT AND DATA MANAGEMENT: A copy of the final report,study protocol, retired stabilityand purity data on the testcompound, tissues, and an aliquot ofthe test compound will be retainedin the LAIR Archives.

TEST SUBSTANCE: DIGL-RP Solid Propellant

INCLUSIVE STUDY DATES: 19 Aug - 14 Sep 85

OBJECTIVE: The objective of this study was to determine themutagenic potential of DIGL-RP solid propellant (LAIR CodeTP057) by using the Ames Salmonella/Mammalian MicrosomeMutagenicity Test.

iii

ACKNOWLE~DGMENTS

CPT John W. Harbell, PhD, MSC; SGT Lillie D. Witcher,BS; SP4 John R.G. Ryabik, BS; Mr. John Dacey; and Ms. JoanneWong provided research assistance.

iv

SIGNATURES OF PRINCIPAL SCIENTISTS INVOLVED IN THESTUDY

We, the undersigned, declare that GLP study number 85014was performed under our supervision, according to theprocedures described herein, and that this report is anaccurate record of the results obtained.

___DON W. KORTE JR, Prw// DATE CONRAD WHEELER, PhD/ DATE

MAJ, MSC DACStudy Director Analytical chemist

STEVEN K. SAN, BA / DATESGT, USAPrincipal Investigator

V

DEPARTMENT OF THE ARMY

LETTERMAN ARMY INSTITUTE OF RESEARCH

PRESIDIO OF SAN FRANCISCO, CALIFORNIA 94129-6800

ATTENTION OF:

SGRD-ULZ-QA (70-in) 15 September 1988

MEMORANDUM FOR RECORD

SUBJECT: GLP Compliance for GLP Study 85014

1. This is to certify that in relation to LAIR GLP Study85014, the following inspections were made:

16 August 1985 - Protocol Review11 September 1985 - Plate Incorporation13 September 1985 - Plate Counting

2. The institute report entitled "Mutagenic Potential ofDIGL-RP Solid Propellant in the Ames Salmonella/MammalianMicrosome Mutagenicity Test," Toxicology Series 150, wasaudited on 20 July 1988.

CAROL M . LEWISChief, Quality Assurance

vi

TABLE OF CONTENTS

Abstract ......... q ..........................................

Preface ................................................... iii

Acknowledgments ............................................ iv

Signatures of Principal Scientists .......................... v

Report of the Quality Assurance Unit ....................... vi

Table of Contents ......................................... vii

BODY OF THE REPORT

INTRODUCTION ........................................... 1

Objective of the Study ............................ 2

MATERIALS AND METHODS .................................. 2

Test Compound ..................................... 2Test Solvent ...................................... 2Chemical Preparation .............................. 2Test Strains ...................................... 3Test Format ....................................... 3Data Interpretation ............................... 5Deviations/Changes ................................ 5Storage of Raw Data and Final Report .............. 5

RESULTS ................................................ 5

DISCUSSION ............................................. 9

CONCLUSION ............................................. 9

REFERENCES ............................................ 10

APPENDIX ................................................... 11

Appendix A. Chemical Data ............................ 12Appendix B. Individual Plate Scores ................... 14

OFFICIAL DISTRIBUTION LIST ................................. 17

vii

Mutagenic Potential of DIGL-RP Solid Propellant in theAmes Salmonella/Mammalian Microsome Mutagenicity Test--Sano and Korte

INTRODUCTION

The Department of Defense is considering the use ofdiethyleneglycol dinitrate (DEGDN), triethyleneglycoldinitrate (TEGDN), or trimethylolethane trinitrate (TMETN) asa replacement for nitroglycerin in munition formulations. A"health effects" review conducted for the US Army BiomedicalResearch and Development Laboratory (USABRDL) identifiednumerous gaps in the toxicology database of these compounds(1). Consequently, USABRDL has tasked the Division ofToxicology, LAIR, to conduct an initial evaluation of thehealth effects of DEGDN, TMETN, TEGDN, and two DEGDN-basedpropellants, JA-2 and DIGL-RP. This initial evaluationincludes the Ames mutagenicity test, acute oral toxicitytests in rats and mice, acute dermal toxicity tests inrabbits, dermal and ocular irritation studies in rabbits, anddermal sensitization studies in guinea pigs. This reportcontains the results of a study that assessed the mutagenicpotential of DIGL-RP in the Ames Salmonella/MammalianMicrosome Mutagenicity Test.

The Ames Salmonella/Mammalian Microsome MutagenicityTest is a short-term screening test that utilizes histidlneauxotrophic mutant strains of Salmonella typhimurium todetect compounds that are potentially mutagenic in mammals.A mammalian microsomal enzyme system is incorporated in thetest to increase sensitivity by simulating in vivo metabolicactivation of the test compound. The Ames test is aninexpensive yet highly predictive and reliable test fordetecting mutagenic activity and thus carcinogenic potential(2).

This evaluation of DIGL-RP utilizes a revision of theAmes Salmonella/Mammalian Microsome Mutagenicity Test (3).Two new tester strains, a frame-shift strain (TA97) and astrain carrying an ochre mutation on a multicopy plasmid(TA102), are added to the standard tester set. TA97 replacesTA1537, TA1535 and TA1538 which are removed from therecommended set. TA98 and TA100 are retained.

Sano and Korte--2

Objective or t Sudy

The objective of this study was to determine themutagernLc potential of DIGL-RP solid propellant (LAIR CodeTP057) by using the revised Ames Salmonella/MammalianMicrosome Mutagenicity Test.

MATERIALS AND METHODS

Test Compound

Compound name: DIGL-RP Solid Propellant

Code number: LAIR Code No. TP057

Physical state: Solid

Source: Hercules IncorporatedWilmington, Delaware

Storage: DIGL-RP was received from Radford ArmyAmmunition Plant (Radford, VA) and assigned the LAIR Codenumber TP057. The test compound was stored at roomtemperature (210C) until used.

Chemical Properties/Analysis: Data provided by HerculesInc., characterizing the chemical composition and purity ofthe test material, are presented in Appendix A along withconfirmatory analysis of the test material performed by theDivision of Toxicology, LAIR (Presidio of San Francisco, CA).

Test Solvent

The positive control chemicals and the test compoundwere dissolved in grade I dimethyl sulfoxide (lot 113F-0450)obtained from Sigma Chemical Co. (St. Louis, MO).

Chemical Preparation

DIGL-RP was stored at room temperature (210C) untilused. The solid propellant was ground into a fine powderwith a liquid nitrogen freezer/mill Model # 6700 (SpexIndustries). On the day of dosing, 300 mg of the testcompound was measured into a sterile vial and dissolved in 6ml of grade I dimethyl sulfoxide to achieve a 5% (w/v)solution. Aliquots of this solution were used to dose thetest plates.

Sano and Korte--3

Test Strains

Salmonella strains TA97, TA98, TAI00, and TA102,obtained directly from Dr. Bruce Ames, University ofCalifornia, Berkeley, were used. These strains weremaintained in our laboratory at -800C. Quality control testswere run concurrently with the test substance to establishthe validity of their special features and to determine thespontaneous reversion rate. Descriptions of the strains,their genetic markers, and the methods for strain validationare given in the LAIR SOP, OP-STX-I (4).

Test Format

DIGL-RP was evaluated for mutagenic potential accordingto a revised Ames method (3). A detailed description of themethodology is given in LAIR SOP, OP-STX-1 (4).

Toxicity Tests

Toxicity tests were conducted to determine a sublethalconcentration of the test substance. This toxicity level wasfound by using minimal glucose agar (MGA) plates,concentrations of DIGL-RP ranging from 1.6 x 10-3 mg/plate to5 mg/plate, and approximately 108 cells of TA100 per plate.Top agar containing trace amounts of histidine and biotin wasplaced on the plates. Strain verification was confirmed onthe bacteria, along with a determination of the spontaneousreversion rate. After incubation, the growth on the plateswas observed. Since none of the plates showed a decrease inthe number of macrocolonies (below the number in thespontaneous reversion plates) or an observable reduction inthe density of the background lawn, a maximum "limit" dose of5 mg/plate was used in the mutagenicity test.

Mutagenicity Test

The test substance was evaluated over a 1000-fold rangeof concentrations, decreasing from the minimum toxic level(the maximum or limit dose) by a dilution factor of 5, bothwith and without 0.5 ml of the S-9 microsome fraction. TheS-9 was purchased from Microbiological Associates Inc.(Bethesda, MD). The optimal titer of this S-9, as determinedby Microbiological Associates Inc., was 0.75 mgprotein/plate. After all the ingredients were added, the topagar was mixed, then overlaid on MGA plates. These platescontained 2% glucose and Vogel Bonner "E" Concentrate (5).The water used in this medium and in all reagents came from aTechnic Model 301 Reverse Osmosis Pre-Treatment Water System(Seattle, WA), LAIR SOP, OP-STX-94 (6). Plates were

Sano and Korte--4

incubated upside down in the dark at 370C for 72 hr. Plateswere prepared in triplicate and the average revertant countswere recorded. The average number of revertants at each doselevel was compared to the average number of spontaneousrevertants (negative control). The spontaneous reversionrate (with and without S-9) was monitored by averaging thecounts from two determinations run simultaneously with thetest compound. The spontaneous reversion rate was determinedby inoculating one set of plates before and one set after thetest compound plates so that any change in spontaneousreversion rate during the dosing procedure would be detected.This spontaneous reversion rate was also compared withhistorical values for this laboratory and those cited inMaron and Ames (3). Sterility and strain verificationcontrols were run concurrently. All reagents, testcompounds, and media were checked for sterility by platingsamples of each on MGA media and incubating them at 370C withthe test plates. The Salmonella strains were verified by astandard battery of tests. The integrity of the differentSalmonella strains used in the assay was verified by thefollowing standard tests:

-Lack of growth (inhibition) in the presence of crystalviolet which indicates that the prerequisite alterationof the lipopolysaccharide layer of the cell wall ispresent.

-Growth in the presence of ampicillin-impregnated diskswhich indicates the presence of an ampicillin-resistantR Factor.

-Lack of growth (inhibition) following exposure toultraviolet light which indicates the absence of the DNAexcision-repair mechanism (for all strains exceptTA102).

Four known mutagens were tested as positive controls toconfirm the responsiveness of the strains to the mutationprocess. Each strain must be tested with at least onepositive control but may be tested with several. Thesecompounds, benzofa]pyrene, 2-aminofluorene, 2-aminoanthraceneand 4-nitroquinoline-n-oxide, were obtained from SigmaChemical Co. (St. Louis, MO). The test compound and mutagenswere handled during this study in accordance with thestandards published in NIH Guidelines for the Laboratory Useof Chemical Carcinogens (DHHS Publication No. (NIH) 81-2385,May 1981).

Sano and Korte--5

Data Interpretation

According to Brusick (7), a compound is consideredmutagenic if a positive dose response (correlated doseresponse) over three dose concentrations is achieved with atleast the highest dose yielding a revertant colony countgreater than or equal to twice the spontaneous colony countfor the tester strains TA98 and TAI00. A strong correlateddose response in strain TAI00 without a doubling of theindividual colony count may also be considered positive.

Maron and Ames (3) consider a compound mutagenic intester strains TA97 and TA102 if a correlated dose responseover three concentrations is achieved with the highest doseyielding a revertant colony count greater than or equal totwice the spontaneous colony count.

Deviations/Changes

A 72-hr rather than a 48-hr incubation period was used.According to Maron (personal communication, 1985), theadditional 24-hr growth enables all of the revertant colonies,especially TAI02, to be detected with the colony counter.

Storage of Raw Data and Final Report

A copy of the final report, study protocols, raw data,SOPs, and an aliquot of the test compound will be retained inthe LAIR archives.

RESULTS

On 23 August 1985, the toxicity of DIGL-RP wasdetermined (Table 1). For this experiment all sterility,strain verification, and negative controls were normal (Table1). No toxicity was observed after exposure of the testerstrain (TA100) to the highest dose used (5 mg/plate).

Normal results were obtained for all sterility andstrain verification tests during the Ames Test performed on27-30 August 1985 (Table 2). DIGL-RP did not induce anyappreciable increase in the revertant colony counts relativeto those of the negative control cultures (Table 3).

A copy of the raw data is included in Appendix B.

Sano and Korte--6

TABLE 1: TOXICITY DETERMINATION FOR DIGL-RP

GLP STUDY NUMBER 85014 23 Aug 1985 PERFORMED BY SANO/WONG

TOXICITY DETERMINATION REVERTANT PLATE COUNT (TA100)

CONCENTRATION OF TEST COMPOUND MEAN (ISD) BACKGROUND LAWN*

START RUN NEGATIVE CONTROL 102 (14.0) NL5.0 mg/plate 67 (6.6) NL1.0 mg/plate 71 (7.2) NL0.2 mg/plate 83 (11.1) NL0.04 mg/plate 77 (10.1) NL0.008 mg/plate 86 (1.2) NL0.0016 mg/plate 91 (8.3) NLEND RUN NEGATIVE CONTROL 96 (8.1) NL

STRAIN VERIFICATION FOR TOXICITY DETERMINATION (TA100)

HISTIDINE REQUIREMENT NG*AMPICILLIN RESISTANCE GUV NGCRYSTAL VIOLET

SENSITIVITY (ZONE SIZE) NG (12mm)STERILITY CONTROL NG

STERILITY CONTROL FOR TOXICITY DETERMINATION

MATERIAL TESTED OBSERVATION*

MINIMAL GLUCOSE AGAR PLATES NGTOP AGAR NGDILUENT WATER NGNUTRIENT BROTH NGTEST COMPOUND (HIGHEST DOSE) NG

* NL = Normal Lawn G = Growth NG = No Growth

Sano and Korte--7

TABLE 2: STRAIN VERIFICATION AND STERILITY TESTINGFOR THE MUTAGENICITY DETERMINATION

OF DIGL-RP (TP057)

GLP STUDY NUMBER 85014 12 SEP 1985 PERFORMED BY SANO/WONG

STRAIN VERIFICATION

OBSERVATIONS*STRAINS TA97 TA98 TA 100 TA102

HISTIDINE REQUIREMENT NG NG NG NGAMPICILLIN RESISTANCE G G G GUV REPAIR NG NG NG GCRYSTAL VIOLET

SENSITIVITY NG NG NG NG(ZONE SIZE) (12mm) (10mm) (12mm) (12mm)

STERILITY CONTROL NG NG NG NG

STERILITY CONTROL FOR MUTAGENICITY DETERMINATION

MATERIAL TESTED OBSERVATION*

MINIMAL GLUCOSE AGAR PLATES NGTOP AGAR NGDILUENT WATER NGNUTRIENT BROTH NGTEST COMPOUND (HIGHEST DOSE) NG

* NL = Normal Lawn G = Growth NG = No Growth

Sano and Korte--8

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Sano and Korte--9

DISCUSSION

Certain test criteria must be satisfied before an Amestest can be considered a valid assessment of a compound'smutagenic potential. First, the special features of the Amesstrains must be verified. These features includedemonstration of ampicillin resistance, alterations in the LPlayer, and deficiency in DNA excision-repair (except TA102).Second, the Salmonella strains must be susceptible tomutation by known mutagens. Third, the optimal concentrationof the test compound must be determined by treating TA100with a broad range of doses and observing the potential toxiceffects on formation of macrocolonies and microcolonies. Ifthese tests are performed and expected data are obtained,then the results of the Ames test can be considered valid.

After validation of bacterial strains and selection ofoptimal sublethal doses, DIGL-RP was evaluated in the Amestest. Criteria for a positive response are a correlateddose-response relationship and a twofold increase inrevertant colony counts relative to the respective negativecontrol counts (3,4,7). DIGL-RP did not induce the requisitedose-response relationship or the increase in revertantcolony counts necessary for a positive response. Thus, theresults of this test indicate that DIGL-RP is not mutagenicwhen evaluated in the Ames test.

CONCLUSION

DIGL-RP was evaluated for mutagenic potential in theAmes Test, both in the presence and absence of metabolicactivation, and did not induce a positive mutagenic responseunder conditions of this study.

Sano and Korte--10

REFERENCES

1. Holleman JW, Ross RH, Carroll JW. Problem definitionstudy on the health effects of diethyleneglycoldinitrate, triethyleneglycol dinitrate, andtrimethylolethane trinitrate and their respectivecombustion products. Frederick, Maryland: US ArmyMedical Bioengineering Research and DevelopmentLaboratory, 1983, DTIC No. ADA 127846.

2. Ames BN, McCann J, Yamasaki E. Methods for detection ofcarcinogens and mutagens with Salmonella/MammalianMicrosome Mutagenicity Test. Mutation Res 1975;31:347-364.

3. Maron DM, Ames BN. Revised methods for the SalmonellaMutagenicity Test. Mutation Res 1983;113:173-215.

4. Ames Salmonella/Mammalian Microsome Mutagenesis Test.LAIR Standard Operating Procedure OP-STX-I, Presidio ofSan Francisco, California: Letterman Army Institute ofResearch, 15 November 1983.

5. Vogel HJ, Bonner DM. Acetylornithinase of E. coli:Partial purification and some properties. J Biol Chem1956;218:97-106.

6. Operation of the Technic Model 301 Reverse Osmosis Pre-Treatment Water System and the Corning Model MP-1 GlassStill. LAIR Standard Operating Procedure OP-STX-94,Presidio of San Francisco, California: Letterman ArmyInstitute of Research, 29 July 1985.

7. Brusick D. Genetic toxicology. In: Hayes AW, ed.Principles and methods of toxicology. New York: RavenPress, 1982:223-272.

Sano and Korte--1l

Appendix A. Chemical Data ............................ 12Appendix B. Individual Plate Scores .................. 14

Sano and Korte--12

Appendix A: CHEMICAL DATA

Name of Test Substance: DIGL-RP Solid Propellant

Composition/Analytical Data: See appended data sheet for

information supplied by source

LAIR Code No.: TP057

Physical State: Solid Black Cylinders

Preparation of Test Substance for Dosing:

The cylinders of DIGL were ground to a finepowder under liquid nitrogen using a Spex freezermill. After grinding, the powder was seivedthrough an 80-mesh screen and dissolved in DMSO.

Source: Radford Army Ammunition Plant, Radford, Virginia(prime contractor: Hercules Inc., Wilmington,Delaware).

Lot No.: RAD83M001S169

Sano and Korte--13

Appendix A (cont.): CHEMICAL DATA

DATA SHEET

FORMULA

DIGL-RP

FinishedPropellant

Ingredient ecna

Nitrocellulose 62.50 ± 2.0013.10% ± 0.05% Nitrogen6-12 seconds viscosity

Diethyleneglycol Dinitrate 36.70 ± 1.50(DEGDN)

0.25Ethyl Centralite (EC) 0.25 ± 0.05

0.25

Akardit II 0.45 ± 0.20

Magnesium Oxide 0.05 Max

Graphite 0.05 Max(Chg 5)

TOTAL: 100.00

S

Sano and Korte--14

Appendix B: INDIVIDUAL PLATE SCORES

TOXICITY DETERMINATION WITH TA100

COMPOUND DOSE/plate PLATE 1 PLATE 2 PLATE 3

NEGATIVE CONTROL 116 88 102

(Start Run)

TP057 5.0 mg 60 68 73

TP057 1.0 mg 69 79 65

TP057 0.2 mg 71 85 93

TP057 0.04 mg 76 68 88

TP057 0.008 mg 87 85 85

TP057 0.0016 mg 94 98 82

NEGATIVE CONTROL 101 87 101(End Run)

Sano and Korte--15

Appendix B (cont.): INDIVIDUAL PLATE SCORES

MUTAGENICITY TESTS WITHOUT S-9

* COMPOUND DOSE/plate TA97 TA98 TA100 TA102

NEG CONTROL 70 27 93 150(start run) 53 30 100 128

61 30 ill 158

NEG CONTROL 53 29 130 145(END RUN) 59 25 113 153

69 28 91 186

NQNO* 2.0 gg 76 257 1156 129475 268 1237 111164 310 1294 1163

TP057 5.0 mg 70 18 84 13071 25 78 12560 29 87 121

TP057 1.0 mg 59 20 95 12363 18 91 10869 19 83 126

TP057 0.2 mg 59 24 80 12060 21 78 10860 18 91 104

TP057 0.04 mg 80 17 83 9870 30 66 13273 28 60 97

TP057 0.008 mg 54 24 81 12253 16 78 12360 25 80 145

TP057 0.0016 mg 43 13 94 15350 26 82 13459 32 90 143

* 4-nitroquinoline-n-oxide

Sano and Korte--16

Appendix B (cont.): INDIVIDUAL PLATE SCORES

MUTAGENICITY TESTS WITH S-9

COMPOUND DOSE/plate TA97 TA98 TAI00 TA102

NEG CONTROL 66 21 90 213(Start Run) 59 24 122 221

70 25 91 224

NEG CONTROL 63 36 88 121(End Run) 64 33 81 126

68 23 91 130

2-aminofluorene 2.0 gg 168 1-02 1035 264173 1644 1140 278176 1615 1179 324

benzolalpyrene 2.0 gg 309 526412 531516 511

2-aminoanthracene 2.0 gg 1847 18191746 16931668 1864

TP057 5.0 mg 63 31 93 21063 24 96 19671 23 101 225

TP057 1.0 mg 72 21 87 19873 22 63 20040 24 94 189

TP057 0.2 mg 70 20 90 20859 27 91 19658 25 86 169

TP057 0.04 mg 68 24 78 18563 24 101 15658 18 92 127

TP057 0.008 mg 65 24 96 18764 34 62 15550 17 95 150

TP057 0.0016 mg 63 30 58 174A 32 96 16671 23 82 147

Sano and Korte--17

Distribution List

Commander CommandantUS Army Biomedical Research and Academy of Health Sciences

Development Laboratory (27) United States ArmyATTN: SGRD-UBZ-C ATTN: Chief, EnvironmentalFort Detrick, Frederick, MD 21701-5010 Quality Branch

Preventive Medicine DivisionDefense Technical Information Center (HSHA-IPM)

(DTIC) (2) Fort Sam Houston, TX 78234ATTN: DTIC-DLACameron Station Commander US Army MaterielAlexandria, VA 22304-6145 Command

A'ITN: AMSCGUS Army Medical Research and 5001 Eisenhower Avenue

Development Command (2) Alexandria, VA 22333ATTN: SGRD-RMI-SFort Detrick, Frederick, MD 21701-5012 Commander

US Army Environmental HygieneCommandant AgencyAcademy of Health Sciences, US Army ATTN: Librarian, HSDH-AD-LATTN: AHS-CDM Aberdeen Proving Ground, MD 21010Fort Sam Houston, TX 78234

DeanChief School of MedicineUSAEHA Regional Division, West Uniformed Services University of theFitzsimmons AMC Health SciencesAurora, CO 80045 4301 Jones Bridge Road

Bethesda, MD 20014ChiefUSAEHA Regional Division, North CommanderFort George G. Meade, MD 20755 US Army Materiel Command

A'ITN: AMCEN-AChief 5001 Eisenhower AvenueUSAEHA Regional Division, South Alexandria, VA 22333Bldg. 180Fort McPherson, GA 30330 HQDA

ATTN: DASG-PSP-ECommander Falls Church, VA 22041-3258USA Health Services CommandATTN: HSPA-P HQDAFort Sam Houston, TX 78234 AITN: DAEN-RDM

20 Massachusetts, NWWashington, D.C. 20314

6/88