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Midterm Review
Fall 2007
Identify the following monomers:
Amino Acids and Proteins
What are the main components of an amino acid?
Amino Acids are monomers that make up ______________
How do amino acids determine the shape of a protein?
How does the shape of a protein determine its function?
Two Amino Acids join to form a peptide bond—a chain of amino acids makes up a peptide or protein
The Main Components of Amino Acids
Contain nitrogen, hydrogen, carbon and oxygen & sometimes Sulpher
(CHON or CHONS) Amino group: NH2 Carboxyl
group: COOH R Group (variable)
There are 20 different amino acids—each with a different R group
The specific amino acids determine the shape of the Protein or Peptides
4 levels of protein structure
PrimarySecondary TertiaryQuaternary
Primary Structure
The unique sequence of amino acids attached by peptide linkages
What could happen if one amino acid is missing or in a different position?
Secondary
Develops when the protein chain takes a particular shape immediately after formation at the ribosome Parts of the chain become folded and/or twisted
The most common shapes: Coiling alpha helix (ά) Folding beta helix (β)
These shapes are permanent and held in place by hydrogen bonds
Tertiary Structure
Precise, compact structure, unique to that protein, which arises when the molecule is further folded
Types of bonds Hydrogen: hydrogen
shared by 2 atoms (weak) Van der Waals: two or
more atoms are very close
Disulphide: strong covalent bond (SH groups)
Ionic: oppositely charged ions
Quaternary Structure
When two or more proteins become held together, forming a complex, biologically active molecule Example hemoglobin, consisting of 4
polypeptide chains held around a non-protein group
Levels of Protein Structure
What is an enzyme
An Enzyme IS a Protein
It is a biological catalyst
Why is shape important to proteins, specifically Enzymes?
Due to its precise shape and distinctive chemical properties, each enzyme is specific for a certain substrate or a VERY small group of substrate molecules.
Shape can be used positively and negatively
Positive If a protein’s active site
and a substrate match, they will bond and react
Negative If a protein’s active site
and an “imposterer” match the intended reaction will not occur
If another site on the protein is filled, the intended reaction will not occur
Illustrate each type of inhibition—Why is protein shape important?
Competitive InhibitionNon-Competitive InhibitionAllosteric Inhibition
End-Product inhibition (negative feedback)
How can a protein lose its shape?
Denaturation is a structural change in a protein that alters its 3-D shape and causes the loss of its biological properties
Cell Theory
1. All organisms are composed of cells(this does not mean that we have some cells--it
means that we are made of cells)
2. All cells come from pre-existing cells(there is no such thing as a cell that comes from
something other than a cell)
3. Cells are the basic unit of structure and function (cells are the smallest unit of life that fulfill all the requirements of life)
Two major types of cells
Prokaryotes Simple Small No nucleus No membrane-bound
organelles Bacteria (archea and
eubacteria Reproduce by binary
fission All unicellular NOT VIRUSES
Eukaryotes More complex Larger Nucleus Membrane-bound
organelles Plants,Animals,
& Fungi,Protists Reproduce by mitosis Unicellular or
multicellular