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8/7/2019 Millicell uMigration Assay Kit
http://slidepdf.com/reader/full/millicell-umigration-assay-kit 1/2
A powerful, slide-based platform
that measures the effects of
chemoattractants on the migration
of adherent single cells through
real-time imaging.
To see how the Millicellµ-migration slide works, visit:www.millipore.com/umigration
Millicell® µ-Migration Assay Kit
Benefts
• Establishes a stable and linear concentration
gradient after 1 hour that lasts ≥48 hours
• Distinguish chemotaxis from random
movement
• Multiparametric analysis enables greater
mechanistic insight
• Enhanced optical imaging for slow- and fast-
migrating cells• Analyze three chemoattractants in parallel for
increased throughput and analytical power
• Ready to use, no assembly needed
A typical example of cells with tracked migration paths. Enhanced
optical imaging makes the µ-migration slide especially suitable for
high-quality and fluorescence microscopy, perfect for observing
single cells.
8/7/2019 Millicell uMigration Assay Kit
http://slidepdf.com/reader/full/millicell-umigration-assay-kit 2/2
70 µm1 mm
8 hrs
24 hrs
48 hrs
Millipore, Millicell, EmbryoMax, and Advancing Life Science Together are registered trademarks
and the “M” mark, Scepter, and Accutase are trademarks of Millipore Corporation.
Lit. No. PR3196EN00 Rev. B 02/11 LS-SBU-10-03833 Printed in U.S.A.
© 2011 Millipore Corporation, Billerica, MA 01821 U.S.A. All rights reserved.
Use the Millicell µ-migration slide to measure parameters
you’ve never been able to quantify before, such as:
• Migration velocity
• Migration paths of individual cells
• Directionality
• Migration index of tracked cells
You won’t get this kind of information with traditional
migration assay platforms such as Boyden chambers or
scratch (wound healing) assays.*
HT-1080, HUVEC, MDA-MB-231, and NIH-3T3 cell lines
have been shown to work effectively with the Millicell
µ-migration kit.
Center of mass [µm]: x = 0.97 y = 6.41
Number of tracks: 80Counts up: 51Counts down: 29
x axis [µm]
y a x i s [ µ m ]
300
200
100
0
-100
-200
-300-300 -200 -100 0 100 200 300
Center of mass [µm]: x = 0.97 y = 37.25
Number of tracks: 78Counts up: 44Counts down: 34
x axis [µm]
y a x i s [ µ m ]
150
100
50
0
-50
-100
-150-150 -100 -50 0 50 100 150
ell Tracks% FCS 10% FCS
Side view of a chamber.
0% FCS 10% FCS
Positivex-axismigration
Positivey-axismigration
M i g r a t i o n v e
l o c i t y ( µ m / m i n )
0.14
0.12
0.1
0.08
0.06
0.04
0.02
0
-0.02
The Millicell µ-migration slide establishes a stable linear
concentration gradient across the observation window th
lasts 48 hours.**
**The calculated maximum concentration is only 70% of the applied
concentration. The final maximum concentration is only 33% of the
applied concentration. Compare this with a Boyden chamber where
very steep gradients may form along a single axis perpendicular to th
surface of the membrane.
Image examples o luorescence measurements
Relative intensity across a 1 mm slit (raw and smoothed data
www.millipore.com
-0.05
0
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
0.2 0.4 0.6 0.8 1
t = 2hrs
t = 4hrs
t = 8hrs
t = 24hr
t = 48hr
Linear (t
Linear (t
Linear (t
Linear (t
Linear (t
n o r m a l i z e d i n t e n s i t y
distance (mm)
HT-1080 cells migrate aster in presence o 10% FCSchemoattractant
*Data was analyzed and graphed using a free Image J software plug-in.RRI IFRMATI
Description Qty/Pk Catalogue No.
NEW Millicell µ-Migration Assay Kit 1 kit (4 slides,
12 assays)
MMA205
Required Equipment/Reagents
Description Qty/Pk Catalogue No.
Accutase™ Cell Dissociation
Solution
100 mL SCR005
Dulbecco’s Phosphate Buffered
Saline, 1X ES Cell Qualified
500 mL BSS-1005-B
EmbryoMax® ES Cell Qualified Ultra
Pure Water, sterile H2O
500 mL TMS-006-B
EmbryoMax ES Cell Qualified Ultra
Pure Water, sterile H2O
100 mL TMS-006-C
Scepter™ Handheld Automated
Cell Counter
1 ea PHCC00000