MINUTES 06M MEETING
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Pre-commercial lines for production of surface nanostructured antimicrobial and anti-biofilm textiles, medical devices and water treatment membranes
Grant agreement no: 720851
MINUTES 6M MEETING
Turin 14-15th of June, 2017
Hosted by PRJ
MINUTES 06M MEETING
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1. General
19 partners participated in this meeting with a total of 35 persons
Three partners were not present: CBD, MAR and SYN
The Governing Board selected the Exploitation Committee to include PRJ, STZ, NSC and CTEC
Tasks 4.1, 4.2 and 4.3 started at M01
The next meeting will be in Valencia, January 2018, hosted by AITEX.
2. Work plan for the next 6 months
WP number 2 Start date or starting event: M01
WP title Production and assembling of the pre-commercial nano-coating lines and production of the novel functional nanoparticles
Participant number
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
Participant short name
UP
C
BIU
-CH
EM
CN
R
UN
IMIB
CE
NT
I
UL
CS
IC
KLO
PE
R
MA
R
DG
A
CD
B
CT
EC
FN
ST
Z
IND
A
ICO
NE
SY
N
AIT
EX
PR
J
NS
C
OS
M
PM per participant 36 39 25 3 48 7 12 8 8 80 4 6 47 7
Gantt
WP/T No.
WPs/Tasks Name Year 1 Year 2 Year 3
Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4
WP1 Ethics D1.1
D1.3
D1.3
D1.3
D1.2
WP2 Production of the pre-commercial lines and the
nanoparticles
T2.1 Optimisation towards scale-up of processes
T2.2 Defining Safety by Process Design for the three pre-
commercial lines
D2.1 D2.2
T2.3 Production of the pre-commercial lines implementing SbD D2.3 D2.4
T2.4 Provision of the novel antibacterial NPs D2.5 D2.6
T2.5 On-going support of the production lines
Summary and action plan for the next 6 months:
Task 2.1: Optimisation towards scale-up of the processes for coating textiles, water filtration membranes and medical devices - Leader BIU, participants UPC, BIU-CHEM, CENTI, KLO, MAR, DGA, CISC and INDA Main achievements
Preliminary work was done on sonochemical coating of ZnO NPs on silicone catheters. Good antibiofilm activity was obtained: S.aureus - complete killing, E.coli - 3 log reduction
BIU has provided KLO with protocol for coating textiles with CuO NPs. Unfortunately, the trials were not successful due to the mechanical problems of KLOPMAN’s machine.
Preliminary work was performed by CENTI on the spray coating of upholstery textiles provided by MAR. The deposition uniformity and parameters optimization was performed by DOE.
Preliminary characterisation of the spray coating line was performed by ICONE upon visit to CENTI facilities. Plan for the next six months
Collaboration with CNR, CENTI for coating their particles on textiles by sonochemical method, using the “throwing stones” mode.
Characterization of the particles obtained from partners and of coated textiles.
Providing KLO with required recipes for roll to roll coating of textiles with metal oxides
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Task 2.2 Defining Safety by Process Design for the three pre-commercial lines - Leader CNR, participants BIU-CHEM, UPC, CENTI, CTEC, INDA, CENTI and ICONE Main achievements
ISTEC-CNR has prepared and sent to BIU, KLO and CENTI a survey for the identification of the process operational tools and of emission exposure scenarios and collected a first set of information
ISTEC-CNR has prepared TiO2@SiO2 nanosol at working pH 2 and 5 to be tested by BIU in the “throwing stones”mode.
ISMAC-CNR tested the antibacterial activity of TiO2@SiO2 nanosol at pH 2 and found encouraging bacterial reduction (about 98%) for TiO2SiO2 1/3 sample, nevertheless only samples at pH 5 have been considered promising in relationship to process and use requirements
CENTI has prepared and sent to BIU and UNIMIB SiO2@TiO2 nanocomposites for testing their antibacterial efficacy, for their characterisation and for evaluation of the coating amount. Additionally, samples of upholstery fabrics treated with the nanocomposites were sent to INDA for their characterisation
Plan for the next six months
Collection of material and carrier matrix properties and exposure limits for NM or parent (bulk) material from SDS or literature/provider sources.
Using information collected on process and material as inputs for the non-testing approach applied to risk assessment and management of pilot plants, using and comparing control banding tools.
Collaboration with BIUlife and UNIMIB for testing antibacterial activity and cytotoxicity of TiO2@SiO2-pH 5 batches.
Task 2.3 Production and assembling of the pre-commercial lines implementing Safety by Process Design and modelling outputs - Leader CTEC, participants CENTI, ICONE, KLO, MAR, DGA, CDB and BIU-CHEM CTEC activities for the next 6 months:
2.3.1 Production and assembling of the US R2R pre-commercial line - Leader CTEC, partners BIU and CTEC Main achievements
The heritage of the SONO machine from the design, performance and the automation has been sheared with ICONE.
A technical support has been provided to KLO in order to perform the additional test for the correlation between the quality of the coating and the speed of textile.
Preliminary design of ultrasonic transducers (longer ones) for treating the fabric in the R2R machine.
. Plan for the next six months
Provide a technical support on SONO machine in order to perform the test on textile coating from 0.5 to 10 m/min.
Technical meeting in order to confirm the architecture of the machine (the description of all the component to be implemented the machine.
o A technical meeting is planned on the 27 and 28th of July at KLO.
Design activities for the sonochemical tank description in collaboration with ICONE (automation of the machine and with INDA (sensors and measurement).
o A technical meeting is planned on first week of September at KLO / ICONE / INDA. 2.3.2 Production and assembling of the spray coating process - Leader CENTI partner MAR Main achievements
The 0.5 meters wide pilot line for the spray coating process has been optimized by CENTI, to define the best operating parameters for the assembly of the pre-commercial line to be implemented in MAR.
CENTI has sent CNR the information related with the data collection on the processed nanomaterials and on the processing lines.
Plan for the next six months
Provide technical support on the spray coating equipment to INDA and ICONE, to define the variables to be
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studied and monitored.
Perform the spray coating of MAR upholstery fabrics, using several coating speeds (from 2-20 m/min).
Technical meeting scheduled with INDA and STZ to study the equipment functioning, the ideal variables to be monitored and the installation of the suitable controllers.
2.3.3 Production and assembling of the batch pre-commercial line - Leader CTEC Main achievements
A preliminary design of the batch pre-commercial line has been done by CTEC consisting of a static transducer above a moving tank.
The inputs data’s of the lab-scale machine Plan for the next six months
Design activities on the batch pre-commercial according to o The ultrasonic transducer solution to be implemented. o The electrical inputs / outputs for the automation control
Task 2.4 Provision of the novel antibacterial NPs Leader UPC participants BIU, CNR, SYN Main achievements
Novel antibacterial nanospheres (NSs) of quorum quenching enzyme acylase and gentamicin were generated by ultrasound-assisted emulsification. These NSs display a size of 200 nm and a zeta potential of – 40 mV. The hybrid NSs completely eradicated planktonic and biofilm forms of the medically relevant P. aeruginosa at nontoxic concentrations (UPC).
Simultaneous sonochemical/enzymatic process for durable antibacterial coating of cotton hospital fabrics with gallic acid/ZnO NPs was carried out. The antibacterial efficiency of the coated textiles towards Gram-positive S. aureus was maintained even after 60 washing cycles at 75°C. Moreover, after 1 and 7 days contact with human skin fibroblasts, cell viability was not affected, demonstrating that the designed materials are save for medical uses (UPC).
BIU successfully increased the yield of Zn0.11Cu0.89O NPs by changing reaction parameters such as: reaction time, temperature, initial concentration. A gram-scale production of the material is possible.
BIU has developed C-dots doped with Ga NPs that are able to kill very efficiently P. aureginosa bacteria
CNR synthesized polypyrrole (PPy) nanoparticles using iron III as oxidant in water dispersion. The nanoparticles were successfully deposited on cotton fabrics in small batch (without US) and showed excellent antibacterial properties (98.6% bacterial reduction against Escherichia coli following the ASTM E2149-2010). Unfortunately, the pH of the dispersion was not compatible with sonochemical processes, a neutralization must be carried out. Iron did not allow pH changes and led to a stable dispersion because of its precipitation as FeOx. CNR studied a new formulation of PPy synthesis using ammonium persulfate. The dispersion are stable even after neutralization to pH 5 in diluted concentration (0.8 % wt.).
The PPy nanoparticles dispersions at pH 5 and 7 were successfully deposited on cotton fabrics in small batch (without US), but showed poor antibacterial properties (51% bacterial reduction against Escherichia coli following the ASTM E2149-2010). 100 ml of two dispersions at pH 5 and 7 were sent to BIU for deposition testing with US.
Plan for the next six months
Develop NP-coated silicone catheters using acylase and gentamicin and study their efficacy in vitro towards common uropathogens at static and dynamic conditions (UPC).
Develop novel NPs of FSLs will be generated, characterised and used as antibacterial and antibiofilm active agent for sonochemical coating of medical surfaces in the “throwing stones” mode (UPC).
BIU will continue the work of C-dots doped Ga : up-scaling and coating on textiles.
A colorimetric calibration curve (as grey scale) will be done by CNR in order to quantify the amount of PPy deposited on fabrics.
CNR will test alternative formulations (e.g. new dispersing agents) for PPy nanoparticles in order to improve antibacterial properties.
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Internal Meetings
Purpose Partners Place Date
Short term training aimed to extend the knowledge for US-assisted NP coating of textiles, water filtration membranes and medical devices
BIU, UPC BIU July 2017
Technical meeting CTEC, KLO KLO July 27-28, 2017
Technical meeting CTEC, KLO, ICONE
KLO First week of September, 2017
Short term training in DOE and knowledge for the spray coating process for upholstery textiles
CENTI, MAR, INDA, STZ
CENTI September, 2017
Planned Internal deliverables
From To What Purpose Due Date
SYN UPC Function spacer lipids For US-assisted coatings of silicone catheter materials with antibacterial lipids
July 2017
CNR BIU TiO2@SiO2 particles For sonochemical coating on textiles M07
CNR-SMAC BIU Polymer’s solution For sonochemical coating on textiles M07
Gantt
WP/T WPs/Tasks Name Year 1 Year 2 Year 3 Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4
WP3 In situ process monitoring systems
and a smart sensing for products
efficiency monitoring
T3.1 Spectroscopy for in situ monitoring D3.1
T3.2 The automation system D3.5
T3.3 Human Machine Interface” (HMI)
T3.4 Integration of the monitoring and
automation
D3.6
T3.5 Integration within the end products of a smart indicator
D3.3 D3.4
WP number 3 Start date or starting event M01
WP title In situ process monitoring systems and a smart sensing function for monitoring the efficiency of the end products
Participant number
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
Participant short name
UP
C
BIU
-CH
EM
CN
R
UN
IMIB
CE
NT
I
UL
CS
IC
KLO
PE
R
MA
R
DG
A
CD
B
CT
EC
FN
ST
Z
IND
A
ICO
NE
SY
N
AIT
EX
PR
J
NS
C
OS
M
PM per participant
4 7 8 2 11 30 6 3 6 5 68 49 6
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Summary and action plan for the next 6 months:
Task 3.1 Spectroscopy based technology for in situ monitoring of NPs concentration, size, and colloidal stability to increase the robustness and repeatability of the coating processes - Leader INDA, participants STZ, PER, CNR, BIU-CHEM and CENTI 3.1.1 Causal understanding of the coating processes using surface analysis and in situ optical spectroscopy - partners STZ and INDA Main achievements The basic idea to introduce spectroscopy into the pilots is to develop a control system which is based on a process understanding on a molecular level. By means of the wavelength dependent absorption of photons, the chemical identity of the components as well as their concentration can be measured. Using the wavelength dependent measurement of the backscattered photons, the size and morphology of the particles can be determined. Theoretical calculations (see presentation of M06 meeting project website) using e.g. Mie-scattering theory confirm the high sensitivity of this approach especially when the ultraviolet or visible region is used. However, due to the complexity of the overlapping information, modern data analysis like multivariate data analysis (MVA) is needed to extract the important information. First experiments (see 3.1.2) confirm this approach. These experiments show, that the use of a pushbroom imager (PBI) allows to measure:
For the nano-coating processes:
Solution concentration of the different metal ions (NP precursors): identity and concentration For the functionalised products:
NPs concentration on the surface
Homogeneity of the nanocoating quality and its distribution over the coated surfaces Other features like NPs size and possibly also the aggregation status needs further theoretical evaluation and future in situ measurements directly within the plant. Plan for the next six months STZ and INDA will use experimental designs, e.g. a Central Composit Design (CCD), to run experiments at CENTI to create an orthogonal sample set (no or very low correlation between variables of interest). This sample set can be used to further detail the optimum wavelength range for the spectroscopic investigations, the optimum optical set up (e.g. measurement in transmittance or reflectance), as well as the best dynamic range of the PBI. Furthermore, it allows developing a first multivariate calibration model for later inline control. 3.1.2 Optimisation of the in situ PBI multimodal system Leader INDA, participants STZ, BIU-CHEM, and CENTI Main achievements STZ and INDA sent out a questionnaire to the partners to identify for each pilot (see presentation STZ) the:
Critical Quality Attributes (CQA) of the incoming material (liquids and solids)
Critical Process Parameters (CPP) of the three pilots
Critical Control Points (CCP) of the three pilots In parallel, some samples (solutions and coated textiles) from the pilots were sent to INDA and are measured with different optical set ups e.g. transmission or diffuse reflectance at different spectroscopic windows (UV, VIS, NIR). This information allows starting giving specifications of the spectroscopic system. For the evaluation of liquid into the machine :
Spectral wavelength of interest seems to be 350nm till 450nm (Short UV to VIS)
Concentration of NP are high and generates a high scattering and absorption (probably measurement in transmission mode)
To avoid interaction with the process the probe will measure on the pipe For the analysis of tissue:
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Spectral wavelength of interest seems to be 350nm till 450nm (Short UV to VIS)
The distance and lens will have to be defined according to the spatial resolution and tissue width Plan for the next six months The samples of the CCD-design (see 3.1.1) will be used to further optimize the spectroscopic system. As a start, focus will be given to measure off line the spatial distribution of the NPs on the surface of the textiles. Task 3.2 The automation system for controlling critical process parameters, e.g. pH, temperature, coating speed and concentration. Leader ICONE, participants CTEC, UPC, BIU-CHEM, CENTI, MAR, FOR, KLO and DGA Main achievements ICONE has organised several discussion and technical exchanges with CEDRAT in order to understand all technical specification from SONO’s machine. Some pre-tests have been run with KLO to characterise the sonotrodes. Plan for the next six months
Gather missing technical information.
Start developing programme for man machine interface
Start Tests do validate and improve. Task 3.5 Integration within the end products of a smart indicator for shelf life and in-use life time of antimicrobial efficiency Leader CSIC, BIU, UPC, CENTI, CNR and SYN Main achievements Different sensing compounds have been tested. The best one is the Prussian Blue and easiest to use because it is popular as a textile dye. It also has already been validated for biomedicine and biosensing applications. Test of integration have been performed with cotton by using UV and ultrasound. The probe has been tested with different contaminant such as S. aureus, E. coli, Broth with various inoculation concentrations. Plan for the next six months
Optimization of cotton modification with PB
Characterization of PB modified cotton sample (bacteria assay, reflectance, electrochemical)
New sensing material for visual detection (colourless to coloured)
New sensing material for electrical detection.
Planned Internal Meetings
Purpose Partners Place Date
Run designed experiments STZ, INDA, CENTI Portugal Week 37/38
Measure samples and develop first models STZ, INDA France/Germany After week 38
Internal deliverables
From To What Purpose Due Date
STZ, INDA CENTI Suggestion for CCD design Run designed experiments July 2017
CENTI INDA Samples from the CCD design Spectroscopic measurements October 2017
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WP number 4 Start date or starting event: M13
WP title Environment and human safety evaluation of the coating processes and products
Participant
number
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
Participant
short name
UP
C
BIU
-LIF
E
CN
R
UN
IMIB
CE
NT
I
UL
CS
IC
KLO
PE
R
MA
R
DG
A
CD
B
CT
EC
FN
ST
Z
IND
A
ICO
NE
SY
N
AIT
EX
PR
J
NS
C
OS
M
PM per
participant
8 17 12 42 4 16 2 2 2 4 4
Gantt
WP/T WPs/Tasks Name Year 1 Year 2 Year 3
Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4 Q1 Q2 Q3 Q4
WP4 Environment and human safety evaluation of the
coating processes and products
T4.1 Environment safety assessment of the nano-coating
technologies and end products
D4.1
T4.2 Assessment of human toxicology due to exposure of
workers and end users
D4.2
T4.3 Estimation of the potential occurrence of bacterial
resistance
T4.4 Health, safety and environment (HSE) Risk Management
T4.5 Development of safety related SOPs and information sheets
Summary and action plan for the next 6 months:
Task 4.1 Environment safety assessment of the nano-coating technologies and end products Leader UNIMIB partners CNR, UL Main achievements
Set up of environmental and personal monitoring systems for nanos
Identification and set up of the environmental monitoring systems for NP release
Set up of the biological tests for ecotoxicity (FET/FETAX) and human toxicity (see task 4.2) Human reconstructed skin Human alveolar blood barrier
4-Identification of the most promising biocidal NPs to be used for coating and perform antibacterial and toxicity test (M1-M12)
Plan for the next six months
Identify the possible exposure scenarios according to the industrial operating pilots – in collaboration with the companies (in collaboration with WP2 (M9-M12)
Identify the most promising biocidal NPs to be used for coating and perform antibacterial and toxicity test (M1-M12)
Inputs from UL – in connection with SUN and GuideNano projects – to harmonize data collection and methods for exposure, hazard and risk assessment
Task 4.2 Assessment of human toxicology due to exposure of workers and end users Leader UNIMIB partners, DGA, MAR, KLO and UL Main achievements Comparative cytotoxic effects of antibacterial nano metal oxides on human lung and immune cells a- Cytotoxicity, inflammatory response and mode of action of CuO, ZnO and Zn-CuO on A549 and THP-1 cells – preliminary data b- Cytotoxicity and ROS production of TiO2@SiO2 and SiO2@TiO2 - preliminary data Plan for the next six months Abraded airborne NPs, estimated lung deposition and toxicity for Zn-doped CuO (M9-M18)
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Task 3.3 Estimation of the potential occurrence of bacterial resistance to the new antibacterial products - Leader BIU-LIFE, partners UPC, CENTI, CNR and UL Main achievements
Antibacterial characterization of CNR- TiO2@SiO2 samples The pH of the samples was too acidic CENTI- SiO2:TiO2, 1:2 & 1:5
Testing nanomaterials’ resistance
Internal Meetings
Purpose Partners Place Date
Share the research approach and the preliminary results with the companies. Design the 2nd year safety related activities involving the companies
UNIMIB, UPC, CNR, BIU, CENTI, UL, KLO, MAR, DGA, SYN ,PRJ
December 2017
Share the research approach and the preliminary results with the companies. Design the 2nd
year safety related activities involving the companies
UNIMIB, UPC, CNR, BIU, CENTI, UL, KLO, MAR, DGA, SYN ,PRJ
December 2017
Planned Internal deliverables
From To What Purpose Due Date
UNIMIB, BIU-Life
CNR-ISTEC, CENTI
antibacterial and toxicity properties of TiO2@SiO2 and SiO2@TiO2
Refinement of the studies and paper draft (Call conf)
October 2017
UNIMIB, CNR-ISTEC NP emission monitoring – parallel activity of WP2,
Design of the methods and instrumentation for NP monitoring at the workplace
December 2017
UL UNIMIB, CNR-ISTEC
Defining methods for risk assessment
Harmonization of the methodology to generate exposure and toxicity data obtain
December 2017
WP number 5 Start date or starting event: M13
WP title End products manufacturing in relevant industrial environment and characterisation
Participant
number
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
Participant
short name
UP
C
BIU
CN
R
UN
IMIB
CE
NT
I
UL
CS
IC
KLO
PE
R
MA
R
DG
A
CD
B
CT
EC
FN
ST
Z
IND
A
ICO
NE
SY
N
AIT
EX
PR
J
NS
C
OS
M
PM per
participant 46 20 4 35 12 64 18 56 44 24 18 12 10 10 10 58 6 2
Gantt
WP/T WPs/Tasks Name Year 1 Year 2 Year 3
Q
1
Q
2
Q
3
Q
4
Q
1
Q
2
Q
3
Q
4
Q
1
Q
2
Q
3
Q4
WP5 End products manufacturing in relevant industrial environment and
their characterization
T5.1 Production of the end products
T5.2 Characterisation of the end products by common tests
T5.3 Antimicrobial/anti-biofilm efficiency and biocompatibility of the end products D5.2
T5.4 Anti-biofilm performance of water filtration membranes in water scarce area
(Sicily)
T5.5 Conclusion regarding the technology and most efficient NPs
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Summary and action plan for the next 6 months:
Task 5.1. Production of antimicrobial textiles, anti-biofilm membranes and anti-biofilm biocompatible medical devices (Leader: KLO) partners ICONE, INDA, CTEC and NSC. Main achievements KLOPMAN explained the following trials carried out:
Trials performed on the existing machinery installed in Klopman: Recipe used :140 l Ethanol ,240 g Cu(Ac)2 ,16 l Water ,400 ml Ammonia Process ,Temperature above 60°C ,pH 9.1 ,Speed: 0.3 m/min, 3 m/min, 5 m/min, 7 m/min and 10 m/min. Transducer: 2 at maximum power
Textile used in the trial : 60 meters of 65%Polyesetr/35%cotton with fabric 215g/mq Procedure used
Filling manually the sonochemical tank with 140L ethanol
Dissolve 240 gr Cu(Ac)2 in 16L of water and add it to the sonochemical tank
Switch on the circulation and heating system
When the temperature reach 60°C, add approx. 400 ml of Ammonia .The pH will change to above 8. If pH below 8, add ammonia till pH is 8.
When the ammonia will be added, the colour of the solution will be changed from bluish to dark blue and then to brown. Only after the color change and the pH reach 8, the rolling of the fabric should start
These first samples have not been analysed because despite of being treated no coating remained in the surface. This fact was confirmed by BIU. Characterization actions during optimization Task 2.1 During first year check that:
The results obtained increasing the speed or adjusting other parameters are the same than obtained in SONO project (KLO)
How process parameters correlate with antimicrobial efficacy (for CENTI technology)
Having the starting point parameters very well defined are needed for the optimization The treated samples in KLO: o 0.5 m/min o 3 m/min o 5 m/min o 7 m/min o 10 m/min
Antimicrobial activity according to the standard ISO 20743 for 2 bacteria (1 Gram-, 1 Gram+) Escherichia coli/Klebsiella pneumoniae and Staphylococcus aureus, Morphology (SEM, ICP) (samples from CENTI characterised by AITEX. Samples from KLO characterised by BIU. Regarding samples from CENTI, to evaluate how process parameters correlate with antimicrobial efficacy, Plan for the next six months CENTI and KLO will send several treated samples to AITEX obtained during the optimization process. Skype meetings to know the preliminary results and plan the next samples between KLO, CENTI BIU and AITEX, during week 38 in September and week 46 during November. Task 5.2 Characterisation of the end products by common tests - Leader AITEX partners UPC, BIU and CENTI Main achievements Definition of tests and requirements: 1 Upholstery and medical fabrics:
o Tensile strength and elongation (ISO 13934-1), sample size: 30x5cm o Weight measurement (EN 12127), sample size: 20x20cm o Pilling + abrasion resistance (ISO 12945-2 and ISO 12947-3). Size: 20x20cm o Simulated outdoor ageing (only for upholstery fabrics). o CEN ENV 14237 TEXTILES IN THE HEALTHCARE SYSTEM: 2002; (Color fastness light, color fastness washing,
bleach, rubbing, perspiration, dimensional change, and flame retardant) (60x200cm) 2 Water treatment membranes:
o Pressure drop measurement (EN 13443-1) initial filtration efficiency (ASTM D 4751). o Other tests and characterizations on all functional end-products: o SEM, AFM, DSC, ICP-MS and cytotoxicity (ISO 10993-5; size: 15x15cm).
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Task .5.3 Antimicrobial/anti-biofilm efficiency and biocompatibility of the end products Leader AITEX partners, BIU, UPC, 5.3.1 Antibacterial efficiency of the functional textiles Leader AITEX partners: BIU, UPC
Main achievements Bacteria and standards to be used during the project have been defined:
Testing for 4 bacteria, 3 Gram- 1 Gram+, respectively: o –Escherichia coli, o –Klebsiella pneumoniae o –Pseudomonas aeruginosa o –Methicillin resistant Staphylococcus aureus (MRSA), Staphylococcus aureus
ISO 20743 (bacteria) and AATCC 30 (fungi). Sample size for each test: 15x15cm.
Bedding and pyjamas will also be tested as a function of the number of washing cycles: 15, 25, and 60 washing cycles at 75ºC
VERY IMPORTANT: AITEX suggested that the same bacteria and standards must be used in the other WPS tests if we want to have comparable results. All partners agreed. Partners and PTA agreed in that bacteria and standards. AITEX also explained why ISO 20743: 2013: the results are more reproducible than other standards, as AATCC 100, because the method uses a reference fabric (the same fabric without antibacterial treatment) and the International Standard ISO 20743 specifies the interpretation of results in the ANNEX F Plan for the next six months
AITEX has to send all partners the PROCEDURE OF ANTIBACTERIAL ACTIVITY DETERMINATION OF FINISHED TEXTILES ACCORDING TO INTERNATIONAL STANDARD ISO 20743: 2013. It will be send during July 2017. 5.3.2 Antimicrobial/anti-biofilm efficiency and biocompatibility for medical devices Leader UPC Main achievements
Silicone catheter material was successfully coated with antibiofilm active amylase via one step sonochemical process. The developed nanocoating inhibited 60 % of the total E. coli biofilm growth (UPC).
Functional Spacer lipids (FSLs) with antibacterial spermine or selenium functional groups were provided by the partner SYN. The lipids were deposited on silicone catheter material via dip coating, drop casting and sonochemically (one step coating). Ultrasound-coated silicones demonstrated higher antibacterial activity, reducing the S. aureus growth by 2 logs (UPC).
Plan for the next six months
Assess the antibiofilm properties of amylase-coated silicone catheter materials towards other Gram-negative and Gram-positive pathogens. Study the synergistic effect of amylase with known antibacterials (e.g. antibiotics) and develop hybrid coating that will completely eradicate bacteria at lower drug dosage (UPC).
Optimise the conditions of in-situ ultrasound-assisted coating of silicone with antibacterial FSLs to improve their activity towards bacteria in both planktonic and biofilm forms.
Evaluate biocompatibility of the coated medical devices using human cell lines. 5.3.3. Antimicrobial/anti-biofilm efficiency of water treatment membranes Leader AITEX partners: BIU
Same information as the one at the kick off meeting was presented for 5.3.2. and 5.3.3
PTA suggested the inclusion of Pseudomonas aeruginosa determination in both actions.
Internal Meetings
Purpose Partners Place Date
To know the preliminary results and plan the next samples KLO, CENTI, BIU, AITEX Skype Week 38
To know the preliminary results and plan the next samples KLO, CENTI, BIU, AITEX Skype Week 46
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Internal deliverables
From To What Purpose Due Date
AITEX BIU, CENTI, UNIMIB, UPC
Procedure of antibacterial activity determination of finished textiles according to international standard
ISO 20743: 2013
Knowledge about the procedure in order to apply the same in case they carry out a test in this field
July 2017
KLO AITEX,BIU To know the starting point and during the optimization trails
Novemeber-Dececember 2017
CENTI AITEX How process parameters correlate with antimicrobial efficacy (for CENTI technology)
November-December. 2017
WP number 6 Start date or starting event: M06
WP title Sustainability and economic impact
Participant no 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 18 19 20 21 22
Participant short name
UP
C
BIU
CN
R
UN
IMIB
CE
NT
I
UL
CS
IC
KLO
PE
R
MA
R
DG
A
CD
B
CT
EC
FN
ST
Z
IND
A
ICO
NE
SY
N
AIT
EX
PR
J
NS
C
OS
M
PM per participant
6 6 6 6 8 2 4 4 2 4 1 2 2 2 6 29
Gantt
WP/T WPs/Tasks Name Year 1 Year 2 Year 3
Q
1
Q
2
Q
3
Q
4
Q
1
Q
2
Q
3
Q
4
Q1 Q
2
Q
3
Q
4 WP6 Sustainability and economic impact
T6.1 Goal and scope definition, inventory and first partial environmental
profile the up-scaled processes
T6.2 Implementing LCC and LCA studies for the up-scaled coating
processes and products
D6.
1
D6.2
T6.3 Conclusion and design of the optimal full scale manufacturing lines
industries
T6.4 Assessment of standards and regulatory issues
Summary and action plan for the next 6 months:
Task 6.1 Goal and scope definition, inventory phase and first partial environmental profile of the up-scaled processes - Leader PRJ, partners: All listed above Main achievements Life Cycle Assessment framework definition. Planning and preparation of the structure of the analysis to obtain: goal and scope definition, identification of the technical and functional unit in each industrial application (bed linens, urinary catheters, water membranes, upholstery fabrics) Plan for the next six months
Definition of system and system boundaries
Identification of technical unit for each pilot
Data collection
First Data Processing
Inventory refining
MINUTES 06M MEETING
Page 13 of 15
First LCA computations for the three pilots: R2R (SONO); Batch (NOVO), R2R Spray
Development of PROTECT Inventory Tool dedicated to the systems for data collection
Start of refined data processing
Additional refinement of the pilots environmental profiling
Internal Meetings
Purpose Partners Place Date
Sharing of pilot US process Layout and NPs production
MAR, DGA, KLO, CENTI, CTEC, BIU, PRJ
(tele/conf.call) M6-M8
Tech specs and performance of products KLO, CDB, PER, PRJ (tele/conf.call) M6-M8
Follow ups on data inventory collection MAR, DGA, KLO, CENTI, CTEC, BIU, PRJ
(tele/conf.call) M8-M12
MINUTES 06M MEETING
Page 14 of 15
Internal Deliverables
From To What Purpose Due Date
T6.1 prtnrs PRJ Provision of the pilots and processes layouts
Definition of system and system boundaries
M6-M7
T6.1 prtnrs PRJ Detail description of product coating functionalities and targeted performance
Identification of functional unit M6-M7
T6.1 prtnrs PRJ Provision of the pilots dedicated inventories for data collection
Data collection M6-M7
T6.1 prtnrs PRJ Second Data collection inventory refinement M10
KLO, CTEC PRJ R2R US unit layout and process recipe specified
Getting the unit layout and reference process recipe M9
DGA, NSC, BIU, CTEC
PRJ BAT US unit layout and process recipe specified
Getting the unit layout and reference process recipe M9
CENTI,
MAR PRJ
R2R SPR unit layout and process recipes specified
Getting the unit layout and reference process recipe M9
PRJ
KLO, DGA, NSC, BIU CTEC CENTI,MAR
Share of the inventory tool Getting data on process for first LCI phase
M9-M10
WP number 7 Start date or starting event: M13
WP title Dissemination and Exploitation
Participant
number
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22
Participant
short name
UP
C
BIU
CN
R
UN
IMIB
CE
NT
I
UL
CS
IC
KLO
PE
R
MA
R
DG
A
CD
B
CT
EC
FN
ST
Z
IND
A
ICO
NE
SY
N
AIT
EX
PR
J
NS
C
OS
M
PM per
participant 4 4 4 3 4 3 3 2 2 2 2 2 2 2 1 1 2 3 3 2 11 8
Summary and action plan for the next 6 months:
The consortium was very active in dissemination and communication
activities:
Project website established www.protect-h2020.eu
10 Participations in International Conferences and exhibitions
23 publications in the media (press, radio, TV, internet),
One publication in peer reviewed scientific journals – one paper by UNIMIB and BIU
Training
Outside the consortium:
A Lecture at the Nanotoxicology course of Veterinary School, University of
Milan, by UNIMIB
Inside the consortium
Introduction to DoE , Seminar 6M Meeting for PROTECT partners,
By Prof. Dr. Rudolf Kessler; Prof. W. Kessler; Dr. Fabien Chauchard/Dr. Yann Tu
MINUTES 06M MEETING
Page 15 of 15
Exploitation
An extensive business plan for all main end products was developed in D7.13
Main delivered deliverables:
D7.1 - Public Project website - M03 was s established -
D7.13 - First business plans for the new products (four business cases) - M06; responsible RJ with MAR, NCS, DGA, PER,
and KLO
WP number 8 Start date or starting event: M13
WP title Management and Coordination
Participant
number
1 2 3 4 5 6 7 8 9 1
0
1
1
1
2
1
3
1
4
1
5
1
6
1
7
1
8
1
9
2
0
2
1
22
Participant
short name
UP
C
BIU
CN
R
UN
IMIB
CE
NT
I
UL
CS
IC
KLO
PE
R
MA
R
DG
A
CD
B
CT
EC
FN
ST
Z
IND
A
ICO
NE
SY
N
AIT
EX
PR
J
NS
C
OS
M
PM per
participant 19
36
Summary and action plan for the next 6 months:
Main managerial issues were presented and discussed:
Management structure and activities:
o A meeting between CTEC, KLO and the project coordinator Prof.. Tzanov was held during the 06M meeting. The partners agreed that CTEC will take care of the production of the new MUST transducer and 4 conventional transducers and will provide the SONO-tank (all these elements are budgeted) and KLO will provide missing parts from conventional machinery and will cover the price for the manipulation
o The chair of COST CA15114 (Anti-Microbial Coating Innovations to prevent infectious diseases (AMICI)) will be contacted by the PROTECT coordinator Dr. Tzanov to steer dissemination activities
Grant Agreement
Delivered and upcoming deliverables
Next meeting was decided to be in Valencia - date TBD
