Contents

Section 1: Installing the Software······························································1 Section 2: Installing the Driver··································································4

Section 3: Precise Calibration ································································· 11 Section 4: Capturing images with the Capture Window ··························16 Section 5: Capturing Video with the Capture Window ····························18 Section 6: Taking Measurements·····························································19 Section 7: Customizing Your Settings ·····················································20 Section 8: Adding Sound to Your Files ···················································21 Section 9: How to Play Sound·································································21 Section 10: How to Combine Images······················································22 Section 11: Introduction to MCCamera···················································23 Section 12: Frequently Asked Questions·················································28 Note: The intention of this guide is to provide quick instructions to key tasks of the Motic Images Plus software. If more detail is needed or what you are looking for is not contained in this guide, please consult the documentation that comes with your microscope or visit our website http://www.motic.com.

1

Installing the Software

Before installing Motic Images Plus 2.0 ML, please read this chapter carefully, and

perform setup by following the step-by-step instructions.

Preparation for software installation 1. Get your Motic microscope ready, and check that the USB cable of microscope is

not connected to the USB port of the computer.

2. Microscope is powered up.

Installing the software Follow the instructions outlined below to install Motic Images Plus 2.0 ML (images

from Windows 98SE, similar processes for Windows XP, ME and 2000).

Insert the CD-ROM into your CD-ROM drive

and the setup wizard appears automatically.

The Motic Images Plus 2.0 ML Installer

prompts Setup being processed.

Get ready for installation as the installer

informs and click “Next”.

2

To install Motic Images Plus 2.0 ML, you must

accept all the terms of the Software License

Agreement. If you agree, click “Yes” to continue.

If you do not, click “No” and the installation will

be cancelled.

Please fill in the blanks with your name and

company name before continuing with the

installation process. Click “Next”.

The Choose Destination Location dialog box will

appear. Accept the default destination path “C:\

Program Files\ Motic\ Motic Images Plus 2.0” or

click “Browse” to select your own path. Then

click “Next”.

3

Select one of the 3 Setup types to install;

before file copying starts, you may review or

change the settings by clicking “Back”. Begin

program installation by clicking “Next”.

Accept the default program folder “Motic”, or

specify a different folder by entering your own

folder name or selecting from an existing

folder and click “Next”.

Copying of program files begins. (If you wish,

you can choose to install the PDF reader

before the copying of the program files starts.

If you already have the PDF reader installed,

you can skip this part.

4

When all the program files have been copied

to your computer, click “Finish” .It is highly

recommended that you restart your computer.

Installing the Driver

Installing the driver in Windows 2000:

Connect the USB cable to the computer USB

port and the following dialog box appears.

Insert the Motic Images Plus 2.0 ML disk into

the CD-ROM and click “OK”.

Click “Next”.

5

Choose “Search for the…” and click “Next”.

Choose “Specify a Location”, click “Next” and

then click “Browse”.

Choose “2e” under “Driver” in the CD-ROM if

your microscope system is Camera 2 (or select

the corresponding driver according to the

instructions on the CD case, or see the chart

below) and click “OK”.

Camera 2 USB1 only 2e

Camera 3 PAL USB1, S-Video,

RCA

M210

Camera 3 NTSC USB1, S-Video,

RCA

AD640

MC1000/MC2000 USB2.0 only,

1.3/2.0 Megapixels

MC1000/MC2000

MC1001/MC2001 USB2.0 only,

1.3/2.0 Megapixels

Motic MC Camera

V3

MC3001 USB2.0 only, 3.0

Megapixels

Motic MC Camera

V3

MC351/MC352 USB1 only MC351/MC352

6

Click “Next”.

Click “Yes”.

Files are being copied to your computer.

Click “Finish”.

7

Installing the Motic CapL3 Driver (If you have not yet purchased a capture card from us, such as L311, L312, L320 etc,

please skip the following text.)

Left is the installation location of the MoticCap

L3xx, the PC1 slot, to which the contact footing

of the said card is to be inserted.

Press the card down with the input sockets plate

attached to the sockets panel (usually at the

back) of your computer.

Click “Next”.

Choose “Search for a …” and click “Next”.

8

Choose “CD-ROM drives” and click “Next”.

Click “Next”.

Click “Yes”.

Click “Finish”.

9

Click “Yes”.

Click “Next”.

Choose “Search for a …” and click “Next”.

Choose “CD-ROM drives” and click “Next”.

10

Click “Next”.

Click “Yes”.

Click “Finish”.

Restart your computer by clicking “Yes”.

11

Precise Calibration Calibration is a very important step to be taken before using the Motic Images Plus 2.0

ML measuring system as it will ensure the accuracy of the system’s measurements.

Click on the drop-down arrow at the right of the Calibration button on the Measure tool

bar to display the following drop-down menus.

Select the Calibration Wizard command to display the Calibration Wizard window. To

select a calibration method, click on the corresponding tab.

Motic Images Plus 2.0 ML provides three calibration methods, Calibration with Circle,

Calibrate with Scale Cross and Calibrate with Scale Line.

1. Calibrate with Calibration Circle

( i ) To calibrate with a calibration circle, select

the Calibration Wizard command to display the

Calibration Wizard window. Click on the

Calibrate with Calibration Circle tab to display the

corresponding panel.

Click “Load Image” to display the Open Image

files dialog box from which calibration circles

may be selected for calibration. Click “Open’’ to

load the selected image.

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( ii ) Confirm the objective lens used to capture

the selected image, then input the diameter of the

calibration circle. Click “Calibration” to complete

the calibration process.

Note:

Use the appropriate calibration circle slide and magnification suited to your

microscope.

( iii ) The Save Sign dialog box will display

after clicking "Calibration". Click "Save" in the

dialog box to save the calibration results and use

them for measuring.

( iv ) When calibration is complete, click “Close” to close the Calibration Wizard

window.

2. Calibrate with Scale Cross

( i ) To calibrate with a scale cross, select the

Calibration Wizard command to display the

Calibration Wizard window. Select the Calibrate

with Scale Cross tab to display the corresponding

panel.

Click on “Load Image” to display the Open Image

files dialog box from which scale crosses may be

selected for calibration. Click “Open” to load the

selected images.

13

( ii ) When an image is loaded, a circle will

appear on it. Modify its color by clicking the

Circle Color button to display the Color Palette where a color for the circle may be

selected.

First, place the center of the circle (set as "O") in

the middle of the scale cross. Next, place a point

(set as "A") that is horizontal with the center of

the circle on the horizontal line and place a point

(set as "B") that is vertical with the center of the

circle on the vertical line. The magnifier window

under the image window will help users

accurately place the points.

Confirm the objective lens used to capture the

selected image and then input the actual length of

"OA" in the "Width" bar and the actual length of

"OB" in the "Height" bar. Click “Calibration” to

complete the calibration process.

( iii ) The Save Sign dialog box will display after

clicking "Calibration". Click "Save" in the

dialog box to save the calibration results and use

them for measuring.

( iv ) When calibration is complete, click “Close” to close the Calibration Wizard

window.

14

3. Calibrate with Scale Line

( i ) To calibrate with a scale line, select the

Calibration Wizard command to display the

Calibration Wizard window. Select the Calibrate

with Scale Line tab to display the corresponding

panel.

First, select the Horizontal tab to load horizontal

scale lines. Click “Load Image” to display the

Open Image files dialog box from which

horizontal lines may be selected for calibration.

Click “Open” to load the selected lines.

( ii ) Next, select the Vertical tab to load vertical

scale lines. Click “Load Image” to display the

Open Image files dialog box from which vertical

lines may be selected for calibration. Click

“Open” to load the selected lines.

15

( iii ) When the image is loaded, a line will appear

on it. Modify its color by clicking the Line Color

button to display the Color Palette where a color for the line may be chosen.

Drag the two ends of the line (set as "A" on the

horizontal line and as "B" on the vertical line) to

two different scales. The magnifier window under

the image window will help users accurately

place the points.

Confirm the objective lens used to capture the

selected images and then input the actual length

of "A" in the "Width" bar and the actual length of

"B" in the “Height” bar. Click “Calibration’’ to

complete the calibration process.

( iv ) The Save Sign dialog box will display after

clicking "Calibration". Click "Save" in the dialog

box to save the calibration results and use them

for measuring.

( vi ) When calibration is complete, click “Close” to close the Calibration Wizard

window.

16

Capturing Images with the Capture Window

1. When Motic Images Plus 2.0 ML is run, the following workspace will display:

Plus Style Standard Style

2. Click the Capture Window button (pointed out by the red arrow) on the toolbar.

3. The Capture Window will then

display.

17

4. Click the “Capture Current Image” button located on the window toolbar to

capture the current microscope image.

5. After capturing an image, the workspace will display as follows:

Plus Style Standard Style

6. Next, select the Save As command from the File menu to display the Save as

dialogue box. Save the image under a user defined filename and select a file

format from the drop down list provided.

Plus Style Standard Style

Note:

If you experience any problems obtaining a live image, consult Frequently

Asked Questions for troubleshooting tips.

18

Capturing Video with the Capture Window

1. Open the Capture Window and select “Set Time

Limit …” from the Capture menu.

2. In the Time Limit dialogue box, define a time limit

for recording and click “OK”.

3. Click the “Start Capture Video” button from the

toolbar located on the left of the image window.

4. Next, enter a name for your video and click “Save”.

Once you click “Save”, a dialog box will appear,

click “OK” to return to the capture window where

recording will begin.

19

Taking Measurements Note: First, make sure that your system has been properly calibrated prior to taking any

measurements.

Measuring

1. Select any of the Measurement tools by clicking on the corresponding button on the

Measure tool bar.

Plus Style Standard Style

2. From the Measure Control Panel, select the magnification of the objective lens used.

It is set as 1X by default. Click the arrow to display the drop down list of objective

lenses.

3. Move the cursor onto the image window and click and drag the mouse over the area

/ distance to be measured. Change the measurements by clicking and dragging the

mouse on the defined area.

4. Click the right mouse button on another part of the image to display a shortcut

menu. Select “Freeze” or “Lock” to save the measurements. Note: Measurements may be unlocked, but to cancel the freeze status, the Undo

command must be selected.

Erasing a Measurement To erase a measurement, apply the Undo command before saving an image file.

20

Customizing Your Settings

Configuring your measurement settings

1. By clicking the Measure tab on the Control Panel, measurement settings may be

customized.

The measurement units and accuracy parameters (how may digits to display after the

decimal point) may also be set here.

2. After performing a measurement, click the right mouse button to display a shortcut

menu where, by selecting the Unit command, the measurement units may be changed.

If the Properties command is selected, a dialog box will appear which allows users to

change the measurement data colors and line properties.

Configuring your image and video capture settings

Click the Setting button in the Plus Style or the Setting button in the Standard Style to display the following dialog boxes:

Plus Style Standard Style

Here, define the size of the images to be captured, how often to capture a frame, and

other image and video properties.

21

Adding Sound to Your Files Note: To add sound to images, a microphone and a sound card with a microphone input

jack are needed, as well, files must be saved in SFC format (default).

1. Select the Music tab on the Control Panel and click

the “Add Audio to Current Image” button. Click the

left mouse button on the image and a frame will

appear. Click the “Record” button and the Record

dialog box appears. Click the record button on this

dialog to begin recording.

2. Click the “Stop” button to stop recording.

How to Play Sound

1. Select the Music tab on the Control Panel and click the

Playlist button to display the Playlist dialog box. Click

the right mouse button and select the Add command

to add sound files to the list.

2. Click the “Play” button and the sound files will be played in turn.

22

How to Combine Images

1. Click the Album button to display the Album window. Next, click the

“Amalgamation” tab to display the corresponding panel.

2. Click and drag the pictures to be combined into

the Amalgamation panel. Click the

Amalgamation button and select an

amalgamation method to combine them. The

combined picture will then appear in the

preview window.

3. Or, select the pictures to be combined and click

the right mouse button. Select the

Amalgamation command from the shortcut

menu and select a method to combine them.

The combined picture will then appear in the

preview window.

23

Introduction to MCCamera 1) Motic MC1000

1. Before using the Motic MC1000, open the Setting panel and select the

MC1000Camera option to be able to run the Motic MC1000.

2. The Exposure and White Balance operations

will be carried out automatically to make the

quality and color of the image in the preview

window similar to those of actual images. The

Motic MC1000 window will display as shown

on the right:

3. Using the control panel, easily change the quality and effects of the image shown in

the preview window.

4. Click the Capture button to capture the real time image shown in the preview

window. After an image has been captured, the work space will display as shown

below:

The captured image will be saved in the Temp

folder under the current user.

5. Click the Auto Capture button (next to the Capture button) and the program will

capture several images automatically.

24

6. Motic MC1000 also enables users to record

video. Click the Video button on the Tool Bar,

fill in the name and path, and click “OK” to

record and save video.

Recording will begin automatically; click the

STOP button to stop recording.

7. Motic MC1000 enables users to adjust the

Brightness, Contrast, Saturation, etc. of

images. If the brightness is adjusted or the

specimen switched during observation, apply

the Auto exposure and White Balance

commands to adjust the image.

8. The Gain (Color) of images can also be adjusted

through these slider bars.

2) Motic MC2000

1. Before using the Motic MC2000, open the Setting panel and select the

MC2000Camera option to be able to run the Motic MC2000.

25

2. The Exposure and White Balance operations will

be carried out automatically to make the quality

and color of images in the preview window

similar with those of actual images. The Motic

MC2000 window will then display as shown on

the right:

3. Using the control panel, easily change the quality and effects of the image shown in

the preview window.

4. Click the Capture button to capture the real time image shown in the preview

window. After an image has been captured, the work space will display as shown

below:

The captured image will be saved in the Temp

folder under the current user.

5. Click the Auto Capture button (next to the Capture button) and the program will

capture several images automatically.

26

6. Motic MC2000 also enables users to record

video. Click the Video button on the Tool Bar,

fill in the name and path, and click “OK” to

record and save video.

Recording will begin automatically; click the

STOP button to stop recording.

7. Motic MC2000 enables users to adjust the

Brightness, Contrast, Saturation, etc. of

images. If the brightness is adjusted or the

specimen switched during observation, use the

Auto exposure and White Balance commands

to adjust the image.

8. A ROI function is provided as well. Drag the

mouse in the preview window to define a

rectangle. Preview the defined region by

clicking the ROI button. Click the button again

to restore the full image.

27

9. Motic MC2000 also provides a Real-time Image Preview function. Click the

button on the Tool Bar to be able to select different filters with which to

process either the whole image or just the defined region.

10. Motic MC2000 has the ability to capture a clear, sharp image even when there is

insufficient illumination.

28

Frequently Asked Questions (FAQ’S) Q: “I’ve installed the software, made sure that the viewing lever is pulled out (if

applicable) and still no image is produced using the digital microscope. Why?” (For

Camera 3 users)

A: In order to view images, the default video setting must be set to S-Video. To check

if your settings are correct: • Open the Motic DS Capture window.

• Select the “Options” menu.

• Select the “Video Crossbar” option.

• Make sure the Input Video Source is set to S-Video and then click the OK

button.

Q: “When I put the CD in the CD-ROM drive, nothing happens; the software does not

start automatically. Why?”

A: The windows auto-insert detection feature may be disabled. To begin software

installation:

29

1. Double click the My Computer icon

2. Right click on the CD-ROM drive icon

3. Select the AutoPlay feature and installation should begin.

Q: “If spots appear in the Motic DS Capture window while trying to capture an image,

how do I fix this problem?”

A: If spots appear in the Motic DS Capture Window, the

problem probably lies in the resolution settings. When

capturing an image, the resolution defined in the

Properties Dialog Box should be the same as the

resolution defined in the Windows operating system.

For example, if you set the resolution as RGB24, the

resolution of the Windows system should be set to

either RGB24 or RGB32. If you set the resolution as

RGB16, the resolution of the Windows system should

be set as RGB16 as well.

© 1999-2004 Motic China Group Co., Ltd. All rights reserved. Motic is a registered trademark and

service mark of Motic China Group Co., Ltd. Microsoft, Windows logo are registered trademarks of

Microsoft Corporation. All other trademarks are the property of their respective owners.

Design Change: The manufacturer reserves the right to make changes in instrument design in accordance with

scientific and mechanical progress, without notice and without obligation.

SP010323C 20041216