Broad Respiratory Virus Recovery Using Mixed Cell Monolayers Viral respiratory disease is caused by more than just Influenza and RSV.

R-Mix* and R-Mix Too† will allow you to culture and identify many respiratory

viruses not covered by other cells: Adenovirus, Parainfluenza (Types 1, 2, and 3)

and Metapneumovirus in addition to enhanced rapid isolation of Influenza

A & B and RSV.

Rapid Time to Results, Convenient Testing Formats, and Easy to Interpret ResultsDesigned for modern virology labs, R-Mix, R-Mix Too, and Diagnostic HYBRIDS

D3® respiratory staining kits give you next day, gold standard results. This total

package approach decreases turnaround time for Influenza to under 24 hours and

all respiratory viruses to 48 hours, compared to up to 14 days with traditional viral

cultures.

The Gold StandardViral Culture is the Gold Standard of the industry for diagnosing viral

respiratory disease. The use of R-Mix (a mixed monolayer of human

adenocarcinoma cells and mink lung cells) or R-Mix Too (a mixed

monolayer of human adenocarcinoma cells and

Madin-Darby Canine kidney cells) improves the

sensitivity of culture when compared to

other cell types.

R-Mix™ Cells infected with Influenza A, stained with D3 Ultra DFA Respiratory Virus

Screening & ID Kit

For Rapid Respiratory

Virus Cultures - Totally

Tubeless respiratory virus

culture results achieved in a

timeframe that allows clinicians

to treat patients with improved

confidence

R-Mix™ & R-Mix Too™

M i x e d C e l l s ™

*US Patent No. 6,946,291†US Patent No. 6,946,291

A Quidel Company For More Information, Contact Us www.dhiusa.com (866) 344-3477© 2010 Quidel Corporation. All rights reserved.

R-Mix™ & R-Mix Too™

M i x e d C e l l s ™

R-Mix™R-Mix

A549 and Mv1Lu Cells

Vials

96-0101 Shell Vial (no coverslip)

96-0102 Shell Vial (with coverslip)

Cluster Plates

96-2412 24 well/12 fill

96-2418 24 well/18 fill

96-2424 24 well/24 fill

96-4812 48 well/12 fill

96-4818 48 well/18 fill

96-4824 48 well/24 fill

R-Mix Too

A549 and MDCK Cells

Vials

97-0102 Shell Vial (with coverslip)

Cluster Plates

97-2412 24 well/12 fill

97-2418 24 well/18 fill

97-2424 24 well/24 fill

97-4812 48 well/12 fill

97-4818 48 well/18 fill

97-4824 48 well/24 fill

R-Mix Refeed Medium

A defined medium containing trypsin, for use with R-Mix

and R-Mix Too

10-330100 100-mL

10-330500 500-mL

Individual D3 Ultra DFA Reagents

01-013010.v2 D3 Ultra DFA Respiratory Virus

Screening Reagent (10-mL)

01-013102.v2 D3 Ultra DFA Influenza A

Reagent (2-mL)

01-013202.v2 D3 Ultra DFA Influenza B

Reagent (2-mL)

01-013302.v2 D3 Ultra DFA RSV Reagent (2-mL)

01-013402.v2 D3 Ultra DFA Adenovirus

Reagent (2-mL)

01-013502.v2 D3 Ultra DFA Parainfluenza 1

Reagent (2-mL)

01-013602.v2 D3 Ultra DFA Parainfluenza 2

Reagent (2-mL)

01-013702.v2 D3 Ultra DFA Parainfluenza 3

Reagent (2-mL)Individual Kit Components

01-014005 Respiratory Viruses Antigen

Control Slides (5)

01-014010 Respiratory Viruses Antigen

Control Slides (10)

01-11210 2-Well Specimen Screening Slide

with Blotters (100)

01-11220 8-Well Identification Slide with

Blotters (100)

01-015010 Normal Mouse Gamma Globulin DFA

Reagent (10-mL)

01-001025 Wash Solution Concentrate,

40X (25-mL)

01-002015 Mounting Fluid (15-mL)

01-090025 40X PBS Concentrate (25-mL)

D3 Ultra DFA Respiratory Virus Kit

01-010000.v2 D3 Ultra DFA Respiratory Virus

Screening & ID Kit

Includes: Screen (10-mL), Normal Mouse (10-mL), 40X

Wash Solution Concentrate (25-mL), Mounting Fluid

(15-mL), Flu A (2-mL), Flu B (2-mL), RSV (2-mL), Adeno

(2-mL), Para 1 (2-mL), Para 2 (2-mL), Para 3 (2-mL),

Respiratory Virus Antigen Control Slides (5)

D3 Duet™ Kits

01-200000 D3 Duet Influenza A/Resp Virus

Screening Kit

Includes: Screen (10-mL), Normal Mouse (10-mL), 40X

Wash Solution Concentrate (25-mL), Mounting Fluid

(15-mL), Respiratory Virus Antigen Control Slides (5)

01-210000 D3 Duet RSV/Resp Virus

Screening Kit

Includes: Screen (10-mL), Normal Mouse (10-mL), 40X

Wash Solution Concentrate (25-mL), Mounting Fluid

(15-mL), Respiratory Virus Antigen Control Slides (5)

D3 Ultra™ & D3 Duet

Negative Control Influenza A Influenza B

Parainfluenza 1 Parainfluenza 2

Parainfluenza 3 RSV

Adenovirus

R-Mix™ ReadyCells® SystemR-Mix ReadyCells

F-96-0102-24 24 Shell Vials - With Coverslip

R-Mix ReadyCells Refeed and Rinse Medium

05-370100 ReadyCells: R-Mix Refeed

Medium, 100-mL

05-360075 ReadyCells: R-Mix Rinse

Buffer, 75-mL

ReadyCells Heat Block

99-610-009 ReadyCells Heat Block (120 volts)

(used for rapid, controlled thawing

of ReadyCells)

Recommended Protocol

Clinical Specimen for Respiratory Virus Detection

Inoculate 3 R-Mix or 3 R-Mix Too monolayers with 200 to 400-µL of specimen

Centrifuge 700xg, 1-Hour, Incubate @ 35˚ to 37˚Cfor overnight incubation

Stain 1 Vial With Respiratory Virus DFA Screening Reagent

Examine for the Presence of Fluorescent Staining Cells

Stain 2nd Vial @ Day 2 with Respiratory Virus DFA Screening Reagent

No Fluorescent Staining Cells Seen: Report as

“No Respiratory Viruses Detected”

Fluorescent Staining Cells Observed

Scrape a Shell Vial, Spot PBS-Washed Cells Onto 8-Well Slide, Stain w/ Individual Monoclonal Antibodies

Fluorescent Staining Cells Observed

No Fluorescent Staining Cells Observed

A Quidel Company For More Information, Contact Us www.dhiusa.com (866) 344-3477© 2010 Quidel Corporation. All rights reserved.

MKT0031v2010MAR26