ACTIVITIES

DATE ACTIVITY

JULY 5 LAST PPT LECTURE

JULY 9 VIRUTAL LABORATORY ACTIVITY

JULY 12 FIRST EXAMINATION

JULY 16 FREE DAY: LIBRARY WORK ON HOW TO DEVELOP YOUR GMO

JULY 19-23 GROUP PRESENTATION (PPT) -What to Develop -Why Develop -How will you Develop -Dangers or Issues to Expect

JULY 26 APPLICATION OF BIOTECHNOLOGY LECTURE SERIES

REQUIREMENTS

•  GMO DEVELOPMENT PRESENTATION

•  BIOWEEK EXHIBIT

•  FIELD TRIP PORTFOLIO •  Expectations Before the Trip •  Learnings During the Trip •  Advocacy: Spreading knowledge of

Biotechnology to Others

exams

•  Examination 1: INTRODUCTION TO TECHNIQUES IN BIOTECHNOLOGY

•  Examination 2: APPLICATIONS OF BIOTECHNOLOGY

•  Examination 3: LAWS AND INTERNATIONAL GUIDELINES CONCERNING BIOTECHNOLOGY

•  Take-home (online)

LAST MEETING: HOW WERE YOUR GENES ISOLATED?

• Golden Rice

• BT Corn

• Edible Vaccine in Potato

• GMO Soya

• Recombinant Hepatitis B vaccine

www.designingdigitally.com

GENE ISOLATION

www.koihealth.org

Source of Genes Primers will amplify target Gene of Interest

Amplicon for “CONSTRUCT”

www.invitrogen.com

www.sciencedirect.com

POLYMERASE CHAIN REACTION

www.neb.com

www.teachersdomain.org

MAKING YOUR CONSTRUCT

www.addgene.org

EXAMPLES OF RESTRICTION ENZYMES

TRANSFORMATION & SELECTION MARKERS

bio.davidson.edu passel.unl.edu

BACTERIAL CLONING: THE PROCESS

CONFIRMATION OF EXPRESSION

•  HOW WILL YOU KNOW IF THE GENE THAT YOU INSERTED IS EXPRESSED?

viswagenbiotech.com en.wikipedia.org igece.org

www.designingdigitally.com

HOW ABOUT PLANTS?

A plant part Is cultured

Callus grows

Shoots develop Shoots are rooted;

plant grows to maturity

clonal-solutions.com.au

DELIVERING GENES (Agrobacterium & The Gene Gun)

apps.rhs.org.uk users.comcen.com.au

ehow.com weirdasianews.com cbc.ca

Cloning overview

•  Organismal cloning produces one or more organisms genetically identical to the “parent” that donated the single cell

•  may lead to production of stem cells for research and other applications

•  CLONE: genetically identical individuals produced from one parent

toonrefugee.com

Cloning plants

bbc.co.uk

•  A totipotent cell is one that can generate a complete new organism

•  Plant cloning is used extensively in agriculture

Cloning animals

• Nuclear Transfer (e.g. BRANDY (2004) the cow and DOLLY (1997) the sheep) •  the nucleus of an unfertilized egg cell or zygote

is replaced with the nucleus of a differentiated cell

•  Embryo Splitting (production of high value herds and livestock breeds)

Nuclear transfer

skinnerscience.com

Embryo splitting

csus.edu

HUMAN CLONING & STEM CELLS

Should we or Should we NOT?

2 kinds of cloning

ipscell.com

The ivf way to have a baby

Stem cell therapy stemcellsforhope.com

Stem cells for therapy

home.honolulu.hawaii.edu

READING ASSIGNMENT: Success stories and fail stories using

stem cell therapy Be sure to Identify the points of success and points of failure

thinkprogress.org

NEXT MEETING: the virtual laboratory

PER GROUP: 1.  Laptop

2.   Internet Source

ACTIVITY THE VIRTUAL LABORATORY

Main reference: http://learn.genetics.utah.edu

WE WILL NEED AN I-NET CONNECTION

•  http://learn.genetics.utah.edu/content/labs/extraction/

•  http://learn.genetics.utah.edu/content/labs/pcr/

•  http://learn.genetics.utah.edu/content/labs/gel/

•  http://learn.genetics.utah.edu/content/labs/microarray/

•  http://learn.genetics.utah.edu/content/tech/cloning/clickandclone/

FOR DISCUSSION •  What is the significance of DNA isolation?

•  When is a PCR useful?

•  Why do bands appear in an electrophoresis?

•  How can a microarray data reveal gene expression?

•  When do you think cloning is not indicated?

NEXT MEETING: EXAM exam number 1