Expert in immunotechnology, ModiQuest Research is a Contract Research Organisation. Since its establishment in 2004, the company has been the privileged partner of prestigious industrial and academic laboratories for their research, development, diagnostics and therapeutics projects.

ModiQuest Research at a glance

• LifeScience Park Oss• Company headquarters• Highly Educated & Experienced people• Contract Research Organisation• Communication• Proprietary technologies• Focus on Antibody development

Antibody generation with a strong focus on low immunogenic and difficult antigens

Antigen selection

• Peptide design & modeling • Peptide synthesis

• Whole protein

• cDNA immunisation

Antibody Generation

• Polyclonal Antibodies

• Hybridoma Technology, monoclonal antibodies

• B-cell Selection, monoclonal antibodies

• Phage Display, recombinant monoclonal antibodies

Monoclonal Antibodies via Hybridoma Technology

Immunization• Various immunization protocols in multiple species

Fusion• Proprietary electrofusion of spleen cells with myeloma cell line• Cells are seeded in semi-solid medium

ClonePix-FL based cell sorting• Viability/ antibody production/ antigen binding• Individual clones are transferred to 96 well plate

Monoclonal Antibodies via B-cell selection

• Proprietary antigen specific B-cell selection

• Vaccinated, infectious disease, autoimmune • Rabbit, Lama, Mouse, Human

Monoclonal Antibodies via Phage Display

• Human naïve scFv library

• Human disease scFv libraries - e.g. Rheumatoid Arthritis, Multiple Sclerosis, Scleroderma, SLE, Malaria, Chagas

• Chicken, Mouse, Rabbit scFv libraries - naïve and immune

• Lama libraries - naïve and immune

Antibody Optimization

• Fusion Protein• Protein Tagging • Chimerisation• Humanisation• Affinity Maturation• Sequencing

Antibody Production

MQR proprietary expression vectors

Fully human and chimeric antibodies:• Human IgA1, IgM, IgG1, IgG3, and IgG4 (both Kappa & Lambda)• Mouse IgG1, IgG2a, IgG2b, and IgG3• Chicken IgY

Transient production: CHO, HEK-293

Stable cell lines: CHO, HEK-293

Mammalian antibody-expressing cell lines and vectors

Assay Development

• Animal model: CAIA mouse model• ELISA• Flowcytometry• Bio-assay development

DBA/1 mice treated with a mixture of 8 collagen-specific monoclonal antibodies (2.3 mg total). A control antibody was used at the same concentration. LPS was injected on day 7. Pictures are histological preparations of hind paws 25 days after anti-collagen injection.

SO = Safranin O staining; Blue is bone, red is cartilage. HE = Haematoxylin staining; * = inflammatory infiltrate.

Antigen Selection

Antibody Generation

Antibody Optimization

Antibody Production

AssayDevelopment

• Peptide Design & Modeling• Peptide synthesis• # different immunization techniques

• Proprietary fusion method• # different species & strains available• # different techniques on different species available

• Polyclonal• Hybridoma monoclonal• B-cell selection, monoclonal• Phage Display, recombinant monoclonal

• Affinity maturation• # different proprietary vectors available for reformatting and/or chimerization• Tagging or fusion• Humanization• Antibody sequencing via RACE

• Many cell lines available• Transient production• Stable cell line

• Gen amplification• Up to grams scale production

• Functionality study• Binding capacity study• Bio-Assay• Animal model

ModiQuest Research Workflow

Antibody Processing

• Labeling• Purification