Molecular and Cellular Mechanisms of H+ Transport

NATO ASI Series Advanced Science Institutes Series

A series presenting the results of activities sponsored by the NA TO Science Committee, which aims at the dissemination of advanced scientific and technological knowledge, with a view to strengthening links between scientific communities.

The Series is published by an international board of publishers in conjunction with the NATO Scientific Affairs Division

A Life Sciences B Physics

C Mathematical and Physical Sciences

o Behavioural and Social Sciences

E Applied Sciences

F Computer and Systems Sciences

G Ecological Sciences H Cell Biology I Global Environmental

Change

NATo-peo DATABASE

Plenum Publishing Corporation London and New York

Kluwer Academic Publishers Dordrecht, Boston and London

Springer-Verlag Berlin Heidelberg New York London Paris Tokyo Hong Kong Barcelona Budapest

The electronic index to the NATO ASI Series provides full bibliographical references (with keywords and/or abstracts) to more than 30000 contributions from international scientists published in all sections of the NATO ASI Series. Access to the NA TO-PCO DATABASE compiled by the NATO Publication Coordination Office is possible in two ways:

- via online FILE 128 (NATO-PCO DATABASE) hosted by ESRIN, Via Galileo Galilei, 1-00044 Frascati, Italy.

- via CD-ROM "NATO Science & Technology Disk" with user-friendly retrieval software in English, French and German (© WTV GmbH and DATAWARE Technologies Inc. 1992).

The CD-ROM can be ordered through any member of the Board of Publishers or through NATO-PCO, Overijse, Belgium.

Series H: Cell Biology, Vol. 89

Molecular and Cellular Mechanisms of H+ Transport

Edited by

Barry H. Hirst Department of Physiological Sciences University of Newcastle upon Tyne Medical School Newcastle upon Tyne, NE2 4HH, U.K.

Springer-Verlag

Berlin Heidelberg New York London Paris Tokyo

Hong Kong Barcelona Budapest Published in cooperation with NATO Scientific Affairs Division

Proceedings of the NATO Advanced Research Workshop on Molecular and Cellular Mechanisms of H+ Transport, held in York, U.K., July 27 to August 1, 1993.

Additional material to this book can be downloaded from http://extra.springer.com.

ISBN-13:978-3-642-79303-5 e-ISBN-13:978-3-642-79301-1 001: 10.1007/978-3-642-79301-1

Library of Congress Cataloging-in-Publication Data. Molecular and cellular mechanisms of H transport / edited by Barry H. Hirst. p. cm. - (NATO ASI series. Series H, Cell biology; vol. 89) "This volume comprises the proceedings of a NATO Advanced Research Workshop on Molecular and Cellular Mechanisms of W Transport held on 27th July-1st August, 1993, in York, England". Includes bibliographical references and index.lsBN-13:978-3-642-79303-51.Hydrogen ions-Metabolism-Con­gresses. 2. Biological transport, Active-Congresses. 3. Adenosine triphosphatase-Congresses. I. Hirst, Barry H., 1953- . II. NATO Advanced Research Workshop on Molecular and Cellular Mechanisms of W Transport (1993: York, England) III. Series. QP535.H1M65 1994 599'.0192-dc20 94-34255

This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights oftranslation, reprinting, reuse of illustrations, recitation, broadcast­ing, reproduction on microfilrn or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer-Verlag. Violations are liable for prosecution under the German Copyright Law.

© Springer-Verlag Berlin Heidelberg 1994 Soft cover reprint of the hardcover 1st edititon 1994

Typesetting: Camera ready by authors SPIN 10101769 31/3130 - 5 4 3 210 - Printed on acid-free paper

PREFACE

This volume comprises the proceedings of a NATO Advanced Research Workshop on Molecular and Cellular Mechanisms of H+ Transport held on 27th July - 1st August, 1993, in York, England. The Workshop and this volume reviews current knowledge of proton transport mechanisms in man, in organs, including the stomach, kidney and bone. Information on the gastric H+, K+ -ATPase is compared with information on non-gastric transport H+ -ATPases, including similar P-type H+, K+ -ATPases in addition to V-type and F-type H+ -ATPases in a variety of tissues. Contributions include information on similar H+ pumps from plants and yeast where lessons of relevance to mammalian transport A TPases may be learnt. Contributions on other P-type ATPases, Na+, K+- and Ca2+-ATPases, are included as is information on chimeric A TPases. The contributions then focus on other proton transport mechanisms, including proton/anion antiports, symports and channels. The final two areas of consideration, including regulation of proton and bicarbonate transport mechanisms, emphasise cellular mechanisms involved in translocating pumps to the secretory membranes, and a discussion of how specific organs have become adapted to resist environments of high acid concentration.

As with all such events, the success of the Workshop, as illustrated by the contributions in this volume, cannot be attributed to a single person but reflects the concerted efforts of organisers, contributors, participants and administrative help. I would like in particular to thank the members of the International Scientific Advisory Committee for this Workshop who provided incisive insight into both the topics and key contributors for the Workshop. The excellence and timeliness of the contributions in this volume are a reflection of their hard work. The Advisory Committee consisted of John G. Forte (Berkeley, U.S.A.), Eberhard Fromter (Frankfurt, Germany), Andrew Garner (United Arab Emirates), Miguel J.M. Lewin (Paris, France), Heini MUTer (Zurich, Switzerland), George Sachs (Los Angeles, U.S.A.) and William Silen (Boston, U.S.A.). In particular, I would like to extend my thanks to John Forte and Andrew Garner who pushed forward the overall organisation of the meeting and oversaw its format.

The Workshop was made possible through generous sponsorship by the North Atlantic Treaty Organisation under its Advanced Research Workshop programme. In addition, important contributions were made by Astra Hassle (Sweden) and the Wellcome Trust (U.K.), as well as a contribution from SmithKline Beecham Pharmaceuticals (U.K.). The International Science Foundation (New York) provided sponsorship for two scientists from the former Soviet Union countries to enable them to attend the Workshop. The Federation of European Physiological Societies and the Biochemical Society Membrane Group (U.K.) generously sponsored named lectures.

The local arrangements during the Workshop were efficiently and willingly provided by Dr. Julian White and the staff of IFAB communications at the Institute for Applied Biology in the University of York.

Lastly, but by no means least I would like to acknowledge the particularly efficient help that I personally received from Mrs. Angela Bott in both organising the Workshop and corresponding with the participants, and latterly in the editing of this volume.

Barry H. Hirst

Part

icip

ants

at

the

NA

TO

AR

W o

n M

olec

ular

and

Cel

lula

r M

echa

nism

s o

f H

+ T

rans

port

in

Yor

k.

CONTENTS Gastric H+ ,K+ -ATPase

Gene Structure and Regulation of Gastric Proton Pump Masatomo Maeda

The Gastric HIK ATPase 6 Subunit Gene and Transcriptional Pathways in Acid-Secreting Epithelia of the Stomach and Kidney Ian R. van Driel, Paul A. Gleeson, Seong-Seng Tan, & Ban-Hock Toh 11

Functional Expression of Gastric H,K-ATPase in Sf9 Cells Using the Baculovirus Expression System Corne H. W. Klaassen, Tom J.F. Van Uem, Marii!lle P. De Moel, Godelieve L.J. De Caluwe, Herman G.P. Swarts & Jan Joep H.H.M. De Pont 19

Structural and Functional Integrity of H,K-ATPase Depends on Its 6-Subunit ~a~~~c~ n

The Topology of the ex, 6 Subunits of the Gastric H/K ATPase Jai Moo Shin, Krister Bamberg, Marie Besancon, Keith Munson, Frederic Mercier, Dennis Bayle, Steve Hersey & George Sachs 35

Synthetic Peptide Antibody Probes of Membrane Orientation of the Gastric H,K-ATPase Adam Smolka 55

Molecular Determinants of Subunit Assembly and Processing in Na,K-ATPase and H,K-ATPase Kllthi Geering, Philippe Jaunin, Ahmed Beggah & Frederic Jaisser 63

Assembly of Hybrid Pumps from Na,K-ATPase ex Subunits and the H,K-ATPase 6 Subunit Robert A. Farley & Kurt A. Eakle 71

DCCD Inhibition of Cation Binding in the Gastric H,K-ATPase Edd C Rabon & Kent Smillie 79

Mapping of a Cytoplasmic Site of Gastric (H+ ,K+)-A TPase Involved in the Transmission of K+ -Activation Across the Membrane D. Bayle, J.C Robert, A. Soumarmon, R. Brasseur, H.G.P. Swarts, CH. W. Klaassen & J.J.H.H.M. De Pont 89

New Approaches to Inhibition of Gastric Acid Secretion: Development and Mechanism of Omeprazole BjOrn Wallmark 95

K+ -Competitive Inhibitors of the Gastric (H + + K+)-A TPase David J. Keeling 103

Bioactive Peptide Inhibitors and an Endogenous Parietal Cell Protein: Interactions with the Gastric H+/K+ ATPase John Cup poletti, Pingbo Huang & Danuta H. Malinowska

The Gastric H/K-ATPase: The Principle Target in Autoimmune Gastritis Paul A. Gleeson, Ban-Hock Toh, Frank Alderuccio & Ian R. van Driel

111

119

VIII

Non-gastric transport-A TPases

Acidic and Non-Acidic Endosomes in Kidney Epithelial Cells: Their Role in Cell-Specific Membrane Recycling Processes Dennis Brown & Ivan Sabolic

Regulation and Properties of the Coated Vesicle Proton Pump Yu Feng, Melanie Myers & Michael Forgac

Characterization of the Osteoclast Vacuolar H + ATPase: Biochemistry, Subunit Composition and Cloning of Catalytic Subunits Roland Baron, Marcjanna Bankiewicz, Diptendu Chatterjee, Munmun Chakrabony, Pe 'er David, Chris Fabricant & Natividad Hernando

The Renal H-K-ATPase: Function and Expression Charles S. Wingo, Xiaoming ?hou, Adam Smolka, Kirsten Madsen, C. Craig TIsher, Kevin A. Curran, W. Grady Campbell & Brian D. Cain

Apical K+ -ATPase and Active Potassium Absorption in the Distal Colon Henry J. Binder

Amino Acid Residues Involved in Ouabain Sensitivity and Cation Binding Jerry B. Lingrel, James Van Huysse, William O'Brien, Elizabeth Jewell-Motz & Patrick Schultheis

The Use of Site-Directed Mutagenesis to Identify Functional Sites in the Cli+ ATPase

127

137

145

153

163

173

David H. MacLennan, Ilona S. Skerjanc, WilliamJ. Rice, TIp W. Loo & David M. Clarke. 181

Structure-Function Relationships in Transmembrane Segment IV of the Yeast Plasma Membrane [H +j-ATPase Anthony Ambesi & Carolyn W. Slayman 191

The Role of Two Small Proteolipids Associated to the H+-ATPase from Yeast Plasma Membrane Catherine Navarre, Serge Leterme, Michel Ghislain & Andre Goffeau 199

Ion Translocation Stoichiometries of Two Endomembrane H+ -Pumps Studied by Patch Clamp Julia M. Davies, Ian Hunt & Dale Sanders 205

Molecular Dissection of Vacuolar H+ -Pyrophosphatase Philip A. Rea, Eugene J. Kim, Yongcheol Kim & Rui-Guang ?hen 213

F-type H + -ATPase: Catalysis and Proton Transport Atsuko Iwamoto, Hiroshi Omote, Roben K. Nakamoto, Masatomo Maeda & Masamitsu Futai 221

Contribution of Infrared Spectroscopy to the Understanding of the Structure of the Neurospora crassa Plasma Membrane H + -ATPase Erik Goormaghtigh, Laurence Vigneron & Jean-Marie Ruysschaert 229

Partial Reaction Chemistry and Charge Displacement by the Fungal Plasma-Membrane H+-ATPase Clifford L. Slayman, Adam Beni, Michael R. Blatt 237

IX

Proton/anion anti POrts. symports and channels

Molecular Properties, Kinetics and Regulation of Mammalian Na+ /H+ Exchange Chung-Ming Tse, Susan A. Levine, C.H. Chris Yun, Steven R. Brant, Samir Nath, Jacques Pouyssegur & Mark Donowitz 245

Polarized Na/H Exchangers in Colonic Epithelium: A Problem of Balance Marshall H. Montrose 261

The AE Gene Family of Band 3-Related Anion Exchangers Seth L. Alper 267

Proton-Coupled Peptide Transport in the Small Intestine and Kidney Vadivel Ganopathy, Matthias Brandsch & Frederick H. Leibach 275

H+ -Coupled Solute Transport in Cultured Intestinal Epithelia David T. 1hwaites, Barry H. Hirst & Nicholas L. Simmons 283

Measurement of H+ and Ca2+ Extrusion from Single Isolated Cells A. V. Tepikin & O.H. Petersen 297

CFTR Chloride Channels and Pancreatic Ductal Bicarbonate Secretion Barry E. Argent, John P. Win penny & Michael A. Gray 301

Acute Regulation of Na+:HCO; Cotransport System in Kidney Proximal Tubules Manoocher Soleimani, Gwen L. Bizal, Yolanda J. Hattabaugh, Peter S. Aronson & Julia A. Bergman 309

Potassium Dependent H+/HCO; Transport Mechanisms in Cells of Medullary Thick Ascending Limb of Rat Kidney Pascale Borensztein, H. Amlal, M. Froissan, F. Leviel, M. Bichara & M. Paillard 319

Reaulation of proton and BCO; transport

Characterization of Parietal Cell Functional Morphology Utilizing Isolated Gastric Gland Perfusion, Atomic Force and Confocal Microscopy Irvin M. Modlin, Steven J. Waisbren, Walter F. Boron, Carol J. Soroka, James R. Goldenring & John Geibel

Activation of Calpain in Gastric Parietal Cells Xuebiao Yao & J. G. Fone

Stimulation of Gastric Acid Secretion Causes Actin Filament Formation and Development of the Secretory Surface in Frog Oxynticopeptic Cells Susan J. Hagen & Rudite Jansons

Expression of Rat Gastric H +,K + -ATPase in Insect Cells Using a Double Recombinant Baculovirus Marianne Manin & Paul Mangeat

Influence ofF-Actin on Na+/K+/2Cl· Cotransport in Cultured Intestinal Epithelia Jeffrey B. Matthews

329

341

351

359

367

x

Ca2+ Pools and H+ Secretion by the Parietal Cell Fabian Michelangeli, Giovanna Tonorici & Marie-Christine Ruiz 375

Acidophylic Cl' and K+ Channels of the Gastric Parietal Cell: A New Model of Regulated HCl Secretion John Cup poletti, Ann M. Baker & Danuta H. Malinowska 383

Uncoupling of H + and Pepsinogen Secretion in the Amphibian Oxyntopeptic Cell Marie-Christine Ruiz, Bernardo Gonzalez, Maria Jesus Abad & Fabian Michelangeli 391

The Canine Gastric Muscularis Mucosae and Acid Secretion: A Basket Case? P.K. Rangachari, M.J. Muller, T. Prior & R.H. Hunt 401

The Receptors Regulating Acid Secretion On and Off the Parietal Cell K.C. Kent lloyd & A.H. Soli 415

Post-Receptor Signaling Mechanisms in the Gastric Parietal Cell Catherine S. Chew 423

Cellular resistance to acid

Relative Sensitivity to Acid Injury of Different Regions of the Intestinal Tract A. Garner, G. Abu-Hijleh, R. Dib, S.M.A. Bastaki, A.C. Hunter & A. Allen 431

"First-Pass" Protection Against Luminal Acid in Necturus Antral Epithelium E. Kivilaakso, T. Kiviluoto, H. Mustonen & H. Paimela 439

Regulation of pHi in Oxyntic Cells in Intact Sheets of Frog Gastric Mucosa Akinora Yanaka & William Silen 447

Oxyntic cell Na+ IH+ and Cl'/HC03' exchangers Ursula Seidler 455

Duodenal Mucosal Bicarbonate Secretion in Humans Jon 1. Isenberg & Daniel L. Hogan 465

Gastric Bicarbonate Secretion Luca Debellis, Rosa Caroppo, Claudia Iacovelli, Eberhard FrlJmter & Silvana Curci 475

Role of Dopamine in Control of Duodenal Mucosal Bicarbonate Secretion Gunnar FlemstrlJm, Bengt Sllfsten & Lars Knutson 483

Author Index 491