Molecular biology and evolution in genomics era

Li Jun and Frederick C.C. Leung (fcleung@hkucc.hku.hk)5N01, School of Biological Science, The University of Hong Kong

Genotype designation according to phylogenomic tree

Virus evolutionary and epidemiology study

Geographic transmission of virus

Estimation of time of the most recent common ancestor (tMRCA)

Web based tool for PRRSV molecular epidemiology and evolutionary analysis

Genome sequencing and assembly

o Gaps are randomly dispersed in the chromosome. Most of the large gaps reside in RNA gene regions.

o Read coverage along the genome are nearly uniform, with mild random fluctuation.

o Only ~10 potential mis-assembled regions can be detected when sequencing depth is >10x.

RNA seq and differential gene expression analysis

Metagenomics