Molecular biology reagents - Scharlab...

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Molecular biology reagents

The wise choice

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Contents

Introduction 2

Biological buffers 3 Cloning 6 Antibiotics 6 Enzyme substrates 7 DNA/RNA Extraction Products 8 Electrophoresis 10 Agarose and acrylamide 10 Dyes 14 Buffers for electrophoresis 14 Reagents for electrophoresis 16 Hybridization 18 Reagents for hybridization 18 Buffers for hybridization 19 Reference index 29 Appendix 1and Packaging of substances and mixtures) 30 Appendix 2statements 32 Appendix 3 36

MolecularBiology2010-3.indd 1 01/09/10 17:04

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Introduction

Scharlau high purity chemicals are serving analysts since 1949. During these 60 years, the Scharlau product families have been growing to become a comprehensive source of chemicals for industrial laboratories as well as research centers.Although the Scharlau program has always included some products for biochemistry, this new Molecular Biology Catalogue represents a serious commitment to expand our products and services to the life sciences and bioseparation fields.

In this catalogue you will find more than 70 chemicals used in every molecular biology laboratory. Electrophoresis buffers, enzyme substrates, agaroses, chemicals used in DNA /RNA extraction and other general reagents. Most of them are offered in specific qualities for biochemical and molecular biology applications.

All of our molecular biology products are manufactured under ISO 9002 and submitted to strict and specialized quality control analyses that guarantee its use in molecular biology experiments. We pay special attention to purity, low UV absorption and absence of enzymes that could damage nucleic acids or proteins (DNases, RNases and proteases).

We have included the new classification of substances and mixtures according to the new GHS regulations. As a result, new pictograms, hazard statements as well as precautionary statements are associated to every product.

We hope you will find this catalogue useful for your daily work. As always, we will remain open to any suggestion to improve our range of products and services.


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Biological buffers

These buffer substances are soluble in water and aqueous matrixes. Their best buffering capacity is in the pH range 6 to 9.When selecting a buffer it is recommended to follow these guidelines:1. pKa of the buffer substance must be close to the working pH,

because a buffer has 90% of its buffering capacity within 1 pH unit of its pKa.2. Although temperature changes have low effects on the pH value,

it is advisable to prepare the buffer solution at the same temperature that it will be used.

3. Always prepare the buffer solution at the needed concentration. Avoid dilu-tion errors.

HEPES, extra pure HE0101

N-(2-Hydroxyethyl)-piperazine-N’-(2-ethanesulfonic acid)

Use: HEPES is a buffer substance used in the pH range 6,8-8,2. pKa (25°C): 7,5. Storage: Room temperature, dry.

CAS-No. [7365-45-9]Physical/Chemical data:Security/Transport:

HEPES free acid, molecular biology grade HE0100

N-(2-Hydroxyethyl)-piperazine-N’-(2-ethanesulfonic acid)

Use: HEPES is a buffer substance used in the pH range 6,8-8,2. pKa (25°C): 7,5. Storage: Room temperature, dry.




Biological buffers

HEPES, sodium salt, molecular biology grade HE0011

N-(2-Hydroxyethyl)-piperazine-N’-(2-ethanesulfonic acid), sodium salt

Use: Storage: Room temperature.


MOPS, molecular biology grade MO0070

3-(N-Morpholino)propanesulfonic acid

Use: a (25°C): 7,2. Storage: Room temperature, dry.


PIPES free acid, molecular biology grade PI0061

Piperazine-N,N’-bis(2-ethanesulfonic acid)

Use: a (25°C): 6,8. Storage: Room temperature, dry.



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Biological buffers

PIPES, sesquisodium salt, buffer substance PI0062

Piperazine-N,N’-bis(2-ethanesulfonic acid) sesquisodium salt



TAE 10X buffer, molecular biology grade (see Buffers for electrophoresis)

TBE 5X buffer, molecular biology grade (see Buffers for electrophoresis)

Tris, extra pure TR0422

Tris(Hydroxymethyl)aminomethane

Use: Buffer substance pH 8-9. pKa (25°C): 8,06.Storage: Room temperature.


Tris base, molecular biology grade TR0424

Tris(Hydroxymethyl)aminomethane

Use:

Storage: Room temperature.




Tris-HCl, molecular biology grade TR0425

Tris(hydroxymethyl)aminomethane hydrochloride

Use: Buffer substance pH 8-9. pKa (25°C): 8,06.Storage: Room temperature.


Ampicillin, sodium salt, for biochemical purposes AM0468

Use:

Storage: 4°C.


Chloramphenicol, for biochemical purposes CL0025

Use:



6

Biological buffers


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Kanamycin disulphate, molecular biology grade KA0010

Use:

Storage: 4°C.


Tetracycline hydrochloride, for biochemical purposes TE0132

Achromycin

Use:

Storage:


IPTG, molecular biology grade (dioxane free) IP0010

Isopropyl-ß-D-thiogalactopyranoside

Use:

Storage: 5°C.




X-Gal, for biochemical purposes XG0010

5-Bromo-4-chloro-indolyl-ß-D-galactopyranoside

Use:

Storage: -20°C.


Cesium chloride, molecular biology grade CE0121

Use:

Storage: Room temperature, dry.

CAS-No. [7647-17-8]Physical/Chemical data: 3

Security/Transport:

Guanidine hydrochloride, molecular biology grade GU0061

Aminomethanamidine hydrochloride, Carbamidine hydrochloride



88


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Guanidine thiocyanate, molecular biology grade GU0065

Use:



Lithium chloride, molecular biology grade LI0112

Use:



Security/Transport:

Phenol cryst., molecular biology grade FE0484

Phenic acid, Hydroxybenzene, Carbolic acid



Security/Transport:

99



Sucrose, extra pure SA0020

Cane sugar, Saccharose



Sucrose, molecular biology grade SU0030

Cane sugar, Saccharose



Agarose Low EEO, electrophoresis grade AG0030

Use: -


CAS-No. [9012-36-6]Physical/Chemical data: Security/Transport:


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Agarose Medium EEO, electrophoresis grade AG0031

Use:



Agarose High EEO, electrophoresis grade AG0032

Use: Storage: Room temperature, dry.


Agarose Low Melt, electrophoresis grade AG0034

Use:





Agarose High Resolution, electrophoresis grade AG0036

Use: Storage: Room temperature, dry.


Acrylamide, electrophoresis grade AC3345

Acrylic acid amide

Use:

Storage:


Security/Transport:

Acrylamide, molecular biology grade AC3343

Acrylic acid amide

Use:

Storage:


Security/Transport:


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C A U T I O N

Acrylamide is a potent neurotoxin and it is absorbed through the skin. The effects of acrylamide are cumulative. Wear

gloves made of latex or vinyl and a mask when weighing powdered acrylamide and methylenebisacrilamide. Also

wear gloves when handling solutions containing these chemicals.

Although polyacrylamide is considered to be nontoxic, it should be handled with care because of the possibility that

it might contain small quantities of unpolymerized acrylamide.

If precautions are taken, dangerous substances can be handled safely.

N,N’-Methylene-bis-acrylamide, electrophoresis grade BI0091

Bis-acrylamide

Use:

Storage:


N,N’-Methylene-bis-acrylamide, molecular biology grade BI0090

Bis-acrylamide

Use:

most demanding separations. Storage:



Ethidium bromide, for biochemical purposes ET0108

3,8-Diamino-5-ethyl-6-phenyl-phenantridinium bromide, Homidium bromide

Use:



Ethidium bromide, solution 10 mg/ml ET0109

3,8-Diamino-5-ethyl-6-phenyl-phenantridinium bromide, Homidium bromide

Use:

Storage:


TAE 10X buffer, molecular biology grade TA0010

(Tris-Acetate-EDTA)

Use:


Physical/Chemical data: 3

Security/Transport:



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TBE 5X buffer, molecular biology grade TB0010

Tris borate-EDTA

Use:



Security/Transport:

C O M M O N LY U S E D E L E C T R O P H O R E S I S B U F F E R S

Buffer Working solution Concentrated stock solution

Tris-acetate

Tris-borate

Alkaline

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Ammonium persulfate, molecular biology grade AM0371

Ammonium persulfate, Peroxodisulfuric acid diammonium salt

Use:



Security/Transport:

Glycine, extra pure AC0402

Aminoacetic acid, Glycocoll

Use: a pH range of 9,5-10,5.Storage: Room temperature.


Security/Transport:

Glycine, molecular biology grade AC0406

Aminoacetic acid, Glycocoll

Use: a pH range of 9,5-10,5.Storage: Room temperature.


Security/Transport:


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TEMED, r.g. TE0050

N,N,N’,N’-Tetramethylethylenediamine

Use: Storage:


Security/Transport:

Urea, molecular biology grade UR0133

Use:



Security/Transport:

Formamide, r.g. FO0026

Methanamide, Carbamaldehyde, Formic acid amide

Use:



Security/Transport:



Formamide deionized, molecular biology grade FO0027

Methanamide, Carbamaldehyde, Formic acid amide

Use:



Security/Transport:

SDS, molecular biology grade SD0010

Sodium dodecyl sulfate, Lauryl sulfate, sodium salt

Use:

-



SSC 20X buffer, molecular biology grade SS0010

Standard sodium citrate

Use:



Security/Transport:


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SSPE 20X buffer, molecular biology grade SS0015

Saline Sodium Phosphate EDTA

Use:



Security/Transport:

Ammonium acetate, molecular biology grade AM0271

Use: -


CAS-No. [631-61-8]Physical/Chemical data: 3 Security/Transport:

Ammonium chloride, molecular biology grade AM0274

Ammoniac salt



Security/Transport:



Ammonium sulfate, molecular biology grade AM0401

Sulfuric acid diammonium salt

Use: concentrations can be used.Storage: Room temperature.


Security/Transport:

Boric acid, molecular biology grade AC0580



Security/Transport:

Calcium chloride 2 hydrate, molecular biology grade CA0198



Security/Transport:


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Citric acid 1 hydrate, molecular biology grade AC0726

2-Hydroxy-1,2,3-propanetricarboxylic acid monohydrate

Use: Citric acid is used to reduce pH in most buffers.Storage: Room temperature.


Security/Transport:

di-Sodium hydrogenphosphate anhydrous, molecular biology grade SO0329



1,4-Dithiotreytol, molecular biology grade DI1360

DTT, Cleland’s reagent

Use:

-

Storage: 4°C under nitrogen




EDTA, disodium salt 2 hydrate, r.g. AC0965

Ethylenediaminetetraacetic acid, disodium salt

Use:



EDTA, disodium salt, molecular biology grade AC0967


Use:



Etanol, DNase free ET0011


Use:




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Ficoll® 400 FI0009

Use: ®



Formaldehyde, sol. 37%, r.g. FO0011

Formalin solution, Formol, Methanal solution, Methyl aldehyde solution



Security/Transport:

Formaldehyde, sol. 37%, molecular biology grade FO0012

Formalin solution, Formol, Methanal solution, Methyl aldehyde solution



Security/Transport:



Glycerol anhydrous, molecular biology grade GL0028

Glycerin

Use:



Security/Transport:

Magnesium chloride 6 hydrate, molecular biology grade MA0037

Use: 2



Security/Transport:

Magnesium sulphate 7 hydrate, molecular biology grade MA0086

Bitter salts, Epsom salts




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2-Mercaptoethanol, molecular biology grade ME0095

Use:



Security/Transport:

Polyethylene glycol 6000, molecular biology grade PO0066

Carbowax, PEG, Polyoxyethylene glycol

Use: -



Polyvinylpyrrolidone, molecular biology grade PO0080

PVP





Potassium acetate, molecular biology grade PO0112



Security/Transport:

Potassium chloride, molecular biology grade PO0201

Chloro potassium



Security/Transport:

Potassium di-hydrogen phosphate, molecular biology grade PO0262

Potassium biphosphate, Potassium phosphate monobasic, Primary potassium phosphate, Mono-potassium phosphate



Security/Transport:

Sodium acetate anhydrous, molecular biology grade SO0036

Acetic acid sodium salt



Security/Transport:


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Sodium chloride, molecular biology grade SO0230

Salt, Common salt, Rock salt, Sea salt



Security/Transport:

Sodium di-hydrogen phosphate 1 hydrate, molecular biology grade SO0328

Sodium biphosphate, mono-Sodium orthophosphate, Sodium phosphate monobasic



Security/Transport:

tri-Sodium citrate dihydrate, molecular biology grade SO0205





Triton X-100, molecular biology grade TR0447

o-[4(1,1,3,3-Tetramethylbutyl) phenyl] decaoxyethylene, Octylphenol decaethylene glycolether, Polyethylene glycol mono [p-(1,1,3,3-tetramethylbutyl) phenyl] ether



Security/Transport:

Tween 20, molecular biology grade TW0022

Polyoxyethylene sorbitan monolaurate



Security/Transport:

Zinc chloride, molecular biology grade CI0155



Security/Transport:


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Art. No. Description Page

® 400 23

Art. No. Description Page

SO0328 Sodium di-hydrogen phosphate 1 hydrate,

SO0329 di-Sodium hydrogenphosphate anhydrous,


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1

CLP (CLASSIFICATION LABELLING AND PACKAGING) OF SUBSTANCES AND MIXTURES

member states.

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Signal word

Pictograms H and P statements

New label

Year 2007 2008 2009 2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 onwards Classified, labelled and packaged Classified under both DSD and CLP; under DSD. If CLP labelled and packaged under CLP timeline Substances is applied in full Classified, labelled and packaged as well, no DSD under CLP labelling and packaging

force; repeal of Annex I to DSD

20 January 2009

Obligation

to substances1 December 2010

Obligation

to mixtures1 June 2015

Classified, labelled and packaged under DPD. If CLP is applied in full as well, no DPD labelling and packaging

Mixtures


CLASSIFICATION OF SUBSTANCES


1

T A B L E 5 .1 : C L P H A Z A R D C L A S S E S A N D C A T E G O R I E S

Physical hazards

1.4, 1.5, and 1,6)



1

H: Hazard statements Hazard statements for physical hazards

H242 Heating may cause a fire.

-

Explosives Flammable liquids Oxidizing liquids Compressed gases Corrosive to metals

Acute toxicity Skin corrosion Skin irritation CMR 1), STOT 2)Aspiration hazard

Hazardous to theaquatic environment

1) carcinogenic, mutagenic, toxic to reproduction / 2) specific target organ toxicity

Environmenthazards

Physical hazards

Health hazards

C O D E R A N G E S F O R H A Z A R D A N D P R E C A U T I O N A R Y S T A T E M E N T S

H statements P statements

4 00 Storage


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2

Hazard statements for health hazards

H318 Causes serious eye damage.

H319 Causes serious eye irritation.

H320 Causes eye irritation.

H341 Suspected of causing genetic defects.

H351 Suspected of causing cancer.

H370 Causes damage to organs.

Hazard statements for environmental hazards

P: Precautionary statementsPrecautionary statements General

Precautionary statements Prevention

P220c

compounds.

P232 Protect from moisture.



2

Precautionary statements Response

P330 Rinse mouth.

P337 If eye irritation persists:

for breathing.


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2

contaminated

physician.

bandages.

Precautionary statements StorageP401 Store...

P422 Store contents under...

P422a Store contents under inert gas.

P422f Store in nitrogen.

Precautionary statements Disposal

Supplemental hazard informationPhysical properties

Health properties

Environmental properties



3

EC index number

EINECS

VbF

WGK

2 Contaminant

3 Strong contaminant

UN

Packing category

Disposal

Synonyms

3 strong poisons

Container type and capacitySymbols

“Kubitainer”

Cardboard case

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Notes



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