Moli-bank: the Biobank of the Moli-sani project
M. B. Donati, A. De Curtis, L. Iacoviello Laboratori di Ricerca
Fondazione di Ricerca e Cura “Giovanni Paolo II”, Università Cattolica, Campobasso
Science meets the people
An observational cross-sectional and prospective epidemiologic study
on genetics and lifestyle for a better health
Why cardiovascular disease and tumors?
The “common soil” hypothesis
The Moli- sani study identikit
25, 000 people living in Molise Aged 35 years or more Recruitment phase: 2005- 201 0 follow- up every 5 years Study duration … …years 8
March 2005 April 201 0
Recruited 24, 600 participants
Moli-sani recruitment strategy The cohort of the Moli-sani Project recruited from about
200,000 persons, aged >=35 years, resident in the Molise region.
The Molise region covers an area of 4,438 Km2, including 136 towns.
Participants recruited from city-hall registries by a multistage sampling. Firstly townships sampled in major areas of Molise
region, starting from the main cities (Campobasso, Termoli) by cluster sampling,
then, within each township, participants selected by simple random sampling, using electronically generated numbers.
A typical “Moli-sani” day The arrival
Volunteers arrive fasting in the rooms of the Catholic University of Campobasso specifically arranged for the Moli-sani Project
Here volunteers are welcomed by a secretary team that, after making them sign the informed consensus, provides them with a barcode
A typical “Moli-sani” day The biological sample drawing
Blood, in order to analyze several parameters, but also to extract DNA as to study genetic characteristics of the participants Urine, to evaluate the levels of some substances
A typical “Moli-sani” day The clinical visit
Electrocardiogram
Spirometry
Blood pressure
Weight and height (BMI)
Body fat distribution
A typical “Moli-sani” day Questionnaires
Anamnestic questionnaire provides the basic situation of each volunteer from a clinical and lifestyle point of view
Food frequency questionnaire provides information about one of the main environmental factors involved in cardiovascular and tumor risk
Follow-up
1. SDO and ReNCAM started on June 2010 (6 months delay), every 6 months update, CVD, cancer and deaths
2. Phone follow-up, July 2010,deaths, CVD, cancer, diabetes, hypertension and hypercholesterolemia
Who Moli-sani are Classi di Età
35-45 years 46-55 years 56-65 years 66-75 years > 75 years
24%
27%25%
17%
7%
Stefania 90 years & Renato 99 years
Biological samples test Cholesterol, HDL, TG Glucose, C Reactive Protein D-dimers Blood cell count
Biological samples aliquoted and stored in liquid nitrogen
A typical “Moli-sani” day In the Laboratory
Buffy coats for
DNA extraction
700.000 straws
Serum
Plasma citrate
Plasma EDTA
Buffy coats (DNA)
100.000 Urine
The Moli-Bank
MOLI-SANI BIOBANK
• 4,083 goblets filled in • 28 paillettes for each patient 8 red for plasma EDTA 6 blu for plasma citrate 8 yellow for serum 6 green for pellet
MOLI-SANI BIOBANK
• Urinary spot: 3hr-collection in the morning
• 4 vials, 5 ml for each subject
Each sector contains 35 tubes (canisters), able to store, one on the other, 5 goblets. Goblets are plastic cylinders with 12 prisms of different colours, where straws are positioned (3,4).
1 2
3 4
Each tank (1) is divided in four sectors (2) identified by colours.
Stock system
Each prism contains 14 straws, filled with biological materials by using a authomatic filling machine.
Stock system
Moli-sani sample preparation
Bar-cod printing
To archive, retraive and manage a so high number of samples, we have developped a “ad hoc”, home made
software
Sample management in tanks
Sample management at-80°C
Only authorised, trained personnel can enter the biobank through
FINGER PRINT IDENTIFICATION
Safety and control
Moli-bank was realised in
collaboration with BIO-REP e SAPIO
Moli-bank has been inaugurated December 2006 by
Luigi Luca Cavalli Sforza
Chairman: Licia Iacoviello Steering Committee: Maria Benedetta Donati and Giovanni de Gaetano (Chairmen), Simona Giampaoli (Istituto Superiore di Sanità, Rome, Italy) Ethics and data monitoring committee: Jos Vermylen (University of Leuven, Belgium), Chairman, Ignacio De Paula Carrasco (UCSC, Rome, Italy), Enrico Garaci (Roma, Italy) Event adjudicating committee: Deodato Assanelli (Brescia, Italy), Francesco Alessandrini (Campobasso, Italy), Vincenzo Centritto (Campobasso, Italy), Paola Muti (Roma, Italy), Holger Schünemann (Hamilton, Canada), Pasquale Spagnuolo (Termoli, Italy), Dante Staniscia (Termoli, Italy), Sergio Storti (Campobasso, Italy) Bio-Banking: Amalia De Curtis, (Coordinator) Sara Magnacca, Maria Spinelli Scientific and organizing secretariat: Francesco Zito (Coordinator, Campobasso and Termoli, Italy), Americo Bonanni (Campobasso, Italy), Chiara Cerletti (Campobasso, Italy), Amalia De Curtis (Campobasso, Italy), Augusto Di Castelnuovo (Campobasso, Italy), Licia Iacoviello (Campobasso, Italy), Antonio Mascioli (Campobasso, Italy), Marco Olivieri (Campobasso, Italy).
MOLI-SANI Investigators
Irene Di Stefano Questionari Francesca Fanelli Questionari Alessandra Molinaro Questionari Emanuela Plescia Questionari Iolanda Santimone Questionari Anna Sciaretta Questionari Isabella Sorella Questionari Agnieska Pampuch Prelievi Agostino Pannichella Prelievi Antonio R Vizzarri Prelievi Giovanna Galluppo Call Center Dolores D'Angelo Call Center Rosanna Ramacciato Call Center
Americo Bonanni Responsabile Comunicazione Francesca De Lucia Maria Laura Bonaccio
Amalia De Curtis Responsabile Biobanca Cristian Silvestri Federico Marracino Sara Magnacca
Genetica Molecolare Daniela Cugino Mirella Graziano Maria Carmela Latella Monica de Gaetano Gianni Quacquaruccio Iolanda Santimone
Augusto Di Castelnuovo Responsabile Statistica Simona Costanzo Romina Di Giuseppe Francesco Gianfagna
Marco G. Olivieri Responsabile Informatica Maurizio Giacci Dario Petraroia Antonella Padulo
Branislav Vohnout Coordinatore Studio Moli-Family Francesco Gianfagna