Biology 309 (5 cr.)
Location: Halsey 57
Monday, Wednesday, and Friday: 8:00 – 9:00 am
Instructor: Dr. Eric Matson
Office: 253 Halsey Science Building
E-mail: [email protected]
Office Hours: Wed. 1:00 – 3:00 pm and Fri. 10:20 – 11:30 am
Required Text: “Microbiology” 9th ed. by Prescott et al. with Connect access.
You have a couple of options here:
1. The works: Hard copy of 9th
edition bundled with Connect access runs about $237
2. eBook: Electronic copy of 9th
edition bundled with Connect access runs about $85
3. Mix and match: Stand alone access to Connect 9th
edition runs $50, then you are free to
find a used copy of the 8th
edition.
Option 3 is not ideal, but would work since the figures and text in the book haven’t changed
much.
Laboratory: 171 Halsey Science Center
Laboratory Instructors: Dr. Eric Matson, Vicki Verbrick
Purpose: To introduce students to a variety of fundamental concepts and applications in bacteriology and to
provide a foundation for the future study of microbiology.
Requirements: There is no attendance policy. You must have an acceptable and prearranged excuse for missing class or
you will not be able to make-up exams.
In general, class lectures will follow the textbook and the schedule of lectures. It should be noted that
while the book will serve as a framework for lectures, there will be quite a bit of material presented in
class that is not found in the text. You will be responsible for such material on exams. Thus, if you miss
lecture you should obtain notes from a classmate.
Course Grading:
The lecture portion of the course is worth 60% of your grade. The laboratory accounts for the remaining
40%. The final grades will be based upon total points and will use the following criteria:
a) Exams - There will be four lecture exams (100 points each). Exams will be of multiple choice
and short-answer format. In addition homework and in-class assignments will constitute
additional points (100 points total). There will also be one writing project (100 points).
Information about this project will be provided in a separate document. Thus, there will be 600
points possible for the lecture portion of the course.
b) Laboratory – a practical final exam (100 points), lab reports (200 points total) and lab questions
or assignments (100 points) make up the total points in the laboratory portion of the course. Thus,
there will be 400 points possible for the laboratory portion of the course.
Course Grades will be determined using the following scale (final grades may be “curved” depending on
final class average using lab and lecture grades:
92.5 - 100% = A 72.5-77.4% = C
90.0-92.4% = A- 70.0-72.4%= C-
87.5-89.9% = B+ 67.5-69.9%=D+
82.5-87.4% = B 62.5-67.4% = D
80.0-82.4% = C- 60.0-62.4% = D-
77.5-79.9% = C+ <60% = F
NOTE: If you miss an ‘unexcused’ lab session, you will NOT be able to hand in lab questions or
assignments for that lab. If you must miss a lab, please discuss it with your lab instructor.
Lecture Changes Any changes to the course, cancellations, etc. will be posted to the class D2L site or a class e-mail will be sent out using the D2L class roster. You MUST check your e-mail regularly! Handouts, lab modifications, and additional materials will be placed on D2L. Academic Dishonesty If you are caught cheating or engage in other forms of academic dishonesty, you will receive an F on that assignment and be subject to the Student Academic Disciplinary Procedures as outlined in the Student Disciplinary Code (http://www.uwosh.edu/dean). Cheating includes, but is not limited to:
• Copying directly from sources and claiming the information as your own (plagiarism) • Making up information or giving false information • Giving answers to someone or allowing them to copy your work • Possessing a copy of an examination that you should not possess • Turning in work that was completed by someone else • Using notes or other information during an examination • Copying from another student with or without their consent • False excuses to receive due date extensions
Tentative Lecture Schedule
The schedule of topics may change depending on the pace of the course and class interest. Exam dates
are firm! Please note that there will not be time to cover all topics for which you will be responsible.
My aim is to bring clarity to aspects of the text. You will struggle with exams if you do not read and
understand the assigned chapters in the book.
September Topics covered
Week of Sept. 2 Introduction to the course and its organization
Chapter 1: Evolution of Microorganisms and Microbiology
Film: on the tree of life
Week of Sept. 9 Chapter 2: Microscopy
Chapter 3: Bacterial Cell Structure Chapter 4: Archaeal Cell Structure
Week of Sept. 16 Chapter 7: Microbial Growth
Brief review of cell structure
Friday, Sept. 20 Exam 1
Week of Sept. 23 Chapter 8: Control of Microorganisms in the Environment
Chapter 9: Antimicrobial chemotherapy
Week of Sept. 30 Chapter 10: Introduction to Metabolism
Chapter 11: Catabolism
October Topics covered
Week of Oct. 7 Chapter 11: Catabolism
Chapter 12: Anabolism
Week of Oct. 14 Chapter 12: Anabolism
Brief review of metabolism
Friday, Oct. 18 Exam 2
Week of Oct. 21 Chapter 13: Bacterial Genome Replication and Expression
Chapter 14: Regulation of Bacterial Cellular Processes
Week of Oct. 28 Chapter 14: Regulation of Bacterial Cellular Processes
Chapter 16: Mechanisms of Genetic Variation
November Topics covered
Week of Nov. 4 Chapter 17 & 18: Selected topics in biotech and bioinformatics
Week of Nov. 11 Chapter 19: Microbial Taxonomy and the Evolution of Diversity
Brief review of bacterial genetics, biotechnology, and bioinformatics
Friday, Nov. 15 Exam 3
Week of Nov. 18 Chapter 20: The Archaea
Chapter 21, 22, 23, 24: The Bacteria (selected topics)
Week of Nov. 25 Chapter 21, 22, 23, 24: The Bacteria (selected topics)
Thanksgiving Break Nov. 27 – Nov. 1 (No Class)
December Topics covered
Week of Dec. 2 Chapter 28: Biogeochemical Cycling and Global Climate Change
Chapter 35: Pathogenicity and Infection
Week of Dec. 9 Chapter 39: Human Diseases Caused by Bacteria
Chapter 41: Microbiology of Food
Humans as a microbial ecosystem
Wednesday, Dec. 11 Exam 4
Dec. 13 Careers in microbiology
Bio 309 Lab (Fall 2013)
Instructor: Dr. Eric Matson
Office: HS 253
Hours: Wed. 1:00 – 3:00 pm and Fri. 10:30 – 11:30 am
Email: [email protected]
Point Distribution (to be added to lecture points):
1 Laboratory Practical Final Exam (1) 100 100
4 Laboratory Reports Underlined Exercises 200
200
10 Laborty Questions or Assignments (10) 100 100
TOTAL: 400
400
Attendance: No make up labs will be given! Be sure to attend all lab periods
You must be present in lab to get credit for questions and assignments
Lab Manual: A new copy of Biology 309 Laboratory Manual by Gregory T. Kleinheinz
Preparedness: Read and prepare ahead for each lab period
I am there to guide you but not to do the lab for you
September Week of Sept. 2 No Labs
Week of Sept. 9
Mon. Exercise 1: Bright Field Microscopy
Exercise 3: Smear Preparation and Simple Staining
Exercise 4: The Gram Stain
Wed. Exercise 5: The Acid Fast Stain
Additional: Wet Mounts
Week of Sept. 16
Mon. Exercise 6: The Negative Stain
Exercise 7: The Capsule Stain
Additional: Fluorescent microscopy, DAPI staining
Wed. Exercise 8: The Endospore Stain
Week of Sept. 23
Mon. Exercise 9: Pure Culture Technique
Wed. Finish Exercise 9
Exercise 10 (Modified): Enrichment Culture Technique
October
Week of Sept. 30
Mon. Exercise 11: Bacterial Growth Measurement
Wed. Finish Exercise 11
Continue work on Exercise 10
Week of Oct. 7
Mon. Exercise 12: Detection of Oxidase, Calalase and Nitrate reductase
Exercise 13: Hydrogen Sulfide Production and Motility
Exercise 14: Bacterial Motility
Wed. Finish Exercises 12, 13, and 14
Finish work on Exercise 10
Exercise 11 Lab Report is Due
Week of Oct. 14
Mon. Exercise 15: the IMViC Tests
Exercise 16: Coagulase Activity
Wed.
Exercise 17: Triple Sugar Iron Finish Exercises 15, 16, and 17
Week of Oct. 21
Mon. Exercise 18: The API 20E System
Wed. Finish Exercise 18
Exercise 10 Lab Report is Due
Week of Oct. 28
Mon. Exercise 19: Kirby-Bauer Method
Exercise 20: Oxygen and the Growth of Microorganisms
Wed. Finish Exercises 19 and 20
Additional: Actinomycete isolation and testing
November Week of Nov. 4
Mon. Exercise 21: Temperature and Growth of Microorganisms
Exercise 23: UV Irradiation and the Growth of Microorganisms
Wed. Finish Exercises 21 and 23
Continue work on Actinomycete isolation and testing
Week of Nov. 11
Mon. Exercise 24: Enumeration of Microbes from the Environment
Exercise 25: Evaluation of Antiseptics and Disinfectants
Wed. Finish Exercises 24 and 25
Continue work on Actinomycete isolation and testing
Week of Nov. 18
Mon. Exercise 26: Evaluation of Throat and Skin Microbes
Exercise 27: Examination of Microorganisms on Surfaces
Wed. Finish Exercises 26 and 27
Finish work on Actinomycete isolation and testing
Week of Nov. 25
Mon. Exercise 28: Identification of Bacterial Unknown – Dichotomous Keys
(Streak for isolation and perform Gram stain)
Actinomycete Lab Report Due
Wed. No Lab (Thanksgiving break)
December
Week of Dec. 2
Mon. Work on Identification of Bacterial Unknown – Dichotomous Keys
Wed. Work on Identification of Bacterial Unknown – Dichotomous Keys
Laboratory Notebook for bacterial unknown is due in lecture to Dr. Matson on Friday, Dec. 6. (This counts as the last lab report).
Week of Dec. 9
Mon. Lab Practical on Dec. 9
Wed. No Lab