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Biology 309 (5 cr.) Location: Halsey 57 Monday, Wednesday, and Friday: 8:00 9:00 am Instructor: Dr. Eric Matson Office: 253 Halsey Science Building E-mail: [email protected] Office Hours: Wed. 1:00 3:00 pm and Fri. 10:20 11:30 am Required Text: “Microbiology” 9th ed. by Prescott et al. with Connect access. You have a couple of options here: 1. The works: Hard copy of 9 th edition bundled with Connect access runs about $237 2. eBook: Electronic copy of 9 th edition bundled with Connect access runs about $85 3. Mix and match: Stand alone access to Connect 9 th edition runs $50, then you are free to find a used copy of the 8 th edition. Option 3 is not ideal, but would work since the figures and text in the book haven’t changed much. Laboratory: 171 Halsey Science Center Laboratory Instructors: Dr. Eric Matson, Vicki Verbrick Purpose: To introduce students to a variety of fundamental concepts and applications in bacteriology and to provide a foundation for the future study of microbiology. Requirements: There is no attendance policy. You must have an acceptable and prearranged excuse for missing class or you will not be able to make-up exams. In general, class lectures will follow the textbook and the schedule of lectures. It should be noted that while the book will serve as a framework for lectures, there will be quite a bit of material presented in class that is not found in the text. You will be responsible for such material on exams. Thus, if you miss lecture you should obtain notes from a classmate. Course Grading: The lecture portion of the course is worth 60% of your grade. The laboratory accounts for the remaining 40%. The final grades will be based upon total points and will use the following criteria: a) Exams - There will be four lecture exams (100 points each). Exams will be of multiple choice and short-answer format. In addition homework and in-class assignments will constitute additional points (100 points total). There will also be one writing project (100 points). Information about this project will be provided in a separate document . Thus, there will be 600 points possible for the lecture portion of the course.
Transcript

Biology 309 (5 cr.)

Location: Halsey 57

Monday, Wednesday, and Friday: 8:00 – 9:00 am

Instructor: Dr. Eric Matson

Office: 253 Halsey Science Building

E-mail: [email protected]

Office Hours: Wed. 1:00 – 3:00 pm and Fri. 10:20 – 11:30 am

Required Text: “Microbiology” 9th ed. by Prescott et al. with Connect access.

You have a couple of options here:

1. The works: Hard copy of 9th

edition bundled with Connect access runs about $237

2. eBook: Electronic copy of 9th

edition bundled with Connect access runs about $85

3. Mix and match: Stand alone access to Connect 9th

edition runs $50, then you are free to

find a used copy of the 8th

edition.

Option 3 is not ideal, but would work since the figures and text in the book haven’t changed

much.

Laboratory: 171 Halsey Science Center

Laboratory Instructors: Dr. Eric Matson, Vicki Verbrick

Purpose: To introduce students to a variety of fundamental concepts and applications in bacteriology and to

provide a foundation for the future study of microbiology.

Requirements: There is no attendance policy. You must have an acceptable and prearranged excuse for missing class or

you will not be able to make-up exams.

In general, class lectures will follow the textbook and the schedule of lectures. It should be noted that

while the book will serve as a framework for lectures, there will be quite a bit of material presented in

class that is not found in the text. You will be responsible for such material on exams. Thus, if you miss

lecture you should obtain notes from a classmate.

Course Grading:

The lecture portion of the course is worth 60% of your grade. The laboratory accounts for the remaining

40%. The final grades will be based upon total points and will use the following criteria:

a) Exams - There will be four lecture exams (100 points each). Exams will be of multiple choice

and short-answer format. In addition homework and in-class assignments will constitute

additional points (100 points total). There will also be one writing project (100 points).

Information about this project will be provided in a separate document. Thus, there will be 600

points possible for the lecture portion of the course.

b) Laboratory – a practical final exam (100 points), lab reports (200 points total) and lab questions

or assignments (100 points) make up the total points in the laboratory portion of the course. Thus,

there will be 400 points possible for the laboratory portion of the course.

Course Grades will be determined using the following scale (final grades may be “curved” depending on

final class average using lab and lecture grades:

92.5 - 100% = A 72.5-77.4% = C

90.0-92.4% = A- 70.0-72.4%= C-

87.5-89.9% = B+ 67.5-69.9%=D+

82.5-87.4% = B 62.5-67.4% = D

80.0-82.4% = C- 60.0-62.4% = D-

77.5-79.9% = C+ <60% = F

NOTE: If you miss an ‘unexcused’ lab session, you will NOT be able to hand in lab questions or

assignments for that lab. If you must miss a lab, please discuss it with your lab instructor.

Lecture Changes Any changes to the course, cancellations, etc. will be posted to the class D2L site or a class e-mail will be sent out using the D2L class roster. You MUST check your e-mail regularly! Handouts, lab modifications, and additional materials will be placed on D2L. Academic Dishonesty If you are caught cheating or engage in other forms of academic dishonesty, you will receive an F on that assignment and be subject to the Student Academic Disciplinary Procedures as outlined in the Student Disciplinary Code (http://www.uwosh.edu/dean). Cheating includes, but is not limited to:

• Copying directly from sources and claiming the information as your own (plagiarism) • Making up information or giving false information • Giving answers to someone or allowing them to copy your work • Possessing a copy of an examination that you should not possess • Turning in work that was completed by someone else • Using notes or other information during an examination • Copying from another student with or without their consent • False excuses to receive due date extensions

Tentative Lecture Schedule

The schedule of topics may change depending on the pace of the course and class interest. Exam dates

are firm! Please note that there will not be time to cover all topics for which you will be responsible.

My aim is to bring clarity to aspects of the text. You will struggle with exams if you do not read and

understand the assigned chapters in the book.

September Topics covered

Week of Sept. 2 Introduction to the course and its organization

Chapter 1: Evolution of Microorganisms and Microbiology

Film: on the tree of life

Week of Sept. 9 Chapter 2: Microscopy

Chapter 3: Bacterial Cell Structure Chapter 4: Archaeal Cell Structure

Week of Sept. 16 Chapter 7: Microbial Growth

Brief review of cell structure

Friday, Sept. 20 Exam 1

Week of Sept. 23 Chapter 8: Control of Microorganisms in the Environment

Chapter 9: Antimicrobial chemotherapy

Week of Sept. 30 Chapter 10: Introduction to Metabolism

Chapter 11: Catabolism

October Topics covered

Week of Oct. 7 Chapter 11: Catabolism

Chapter 12: Anabolism

Week of Oct. 14 Chapter 12: Anabolism

Brief review of metabolism

Friday, Oct. 18 Exam 2

Week of Oct. 21 Chapter 13: Bacterial Genome Replication and Expression

Chapter 14: Regulation of Bacterial Cellular Processes

Week of Oct. 28 Chapter 14: Regulation of Bacterial Cellular Processes

Chapter 16: Mechanisms of Genetic Variation

November Topics covered

Week of Nov. 4 Chapter 17 & 18: Selected topics in biotech and bioinformatics

Week of Nov. 11 Chapter 19: Microbial Taxonomy and the Evolution of Diversity

Brief review of bacterial genetics, biotechnology, and bioinformatics

Friday, Nov. 15 Exam 3

Week of Nov. 18 Chapter 20: The Archaea

Chapter 21, 22, 23, 24: The Bacteria (selected topics)

Week of Nov. 25 Chapter 21, 22, 23, 24: The Bacteria (selected topics)

Thanksgiving Break Nov. 27 – Nov. 1 (No Class)

December Topics covered

Week of Dec. 2 Chapter 28: Biogeochemical Cycling and Global Climate Change

Chapter 35: Pathogenicity and Infection

Week of Dec. 9 Chapter 39: Human Diseases Caused by Bacteria

Chapter 41: Microbiology of Food

Humans as a microbial ecosystem

Wednesday, Dec. 11 Exam 4

Dec. 13 Careers in microbiology

Bio 309 Lab (Fall 2013)

Instructor: Dr. Eric Matson

Office: HS 253

Hours: Wed. 1:00 – 3:00 pm and Fri. 10:30 – 11:30 am

Email: [email protected]

Point Distribution (to be added to lecture points):

1 Laboratory Practical Final Exam (1) 100 100

4 Laboratory Reports Underlined Exercises 200

200

10 Laborty Questions or Assignments (10) 100 100

TOTAL: 400

400

Attendance: No make up labs will be given! Be sure to attend all lab periods

You must be present in lab to get credit for questions and assignments

Lab Manual: A new copy of Biology 309 Laboratory Manual by Gregory T. Kleinheinz

Preparedness: Read and prepare ahead for each lab period

I am there to guide you but not to do the lab for you

September Week of Sept. 2 No Labs

Week of Sept. 9

Mon. Exercise 1: Bright Field Microscopy

Exercise 3: Smear Preparation and Simple Staining

Exercise 4: The Gram Stain

Wed. Exercise 5: The Acid Fast Stain

Additional: Wet Mounts

Week of Sept. 16

Mon. Exercise 6: The Negative Stain

Exercise 7: The Capsule Stain

Additional: Fluorescent microscopy, DAPI staining

Wed. Exercise 8: The Endospore Stain

Week of Sept. 23

Mon. Exercise 9: Pure Culture Technique

Wed. Finish Exercise 9

Exercise 10 (Modified): Enrichment Culture Technique

October

Week of Sept. 30

Mon. Exercise 11: Bacterial Growth Measurement

Wed. Finish Exercise 11

Continue work on Exercise 10

Week of Oct. 7

Mon. Exercise 12: Detection of Oxidase, Calalase and Nitrate reductase

Exercise 13: Hydrogen Sulfide Production and Motility

Exercise 14: Bacterial Motility

Wed. Finish Exercises 12, 13, and 14

Finish work on Exercise 10

Exercise 11 Lab Report is Due

Week of Oct. 14

Mon. Exercise 15: the IMViC Tests

Exercise 16: Coagulase Activity

Wed.

Exercise 17: Triple Sugar Iron Finish Exercises 15, 16, and 17

Week of Oct. 21

Mon. Exercise 18: The API 20E System

Wed. Finish Exercise 18

Exercise 10 Lab Report is Due

Week of Oct. 28

Mon. Exercise 19: Kirby-Bauer Method

Exercise 20: Oxygen and the Growth of Microorganisms

Wed. Finish Exercises 19 and 20

Additional: Actinomycete isolation and testing

November Week of Nov. 4

Mon. Exercise 21: Temperature and Growth of Microorganisms

Exercise 23: UV Irradiation and the Growth of Microorganisms

Wed. Finish Exercises 21 and 23

Continue work on Actinomycete isolation and testing

Week of Nov. 11

Mon. Exercise 24: Enumeration of Microbes from the Environment

Exercise 25: Evaluation of Antiseptics and Disinfectants

Wed. Finish Exercises 24 and 25

Continue work on Actinomycete isolation and testing

Week of Nov. 18

Mon. Exercise 26: Evaluation of Throat and Skin Microbes

Exercise 27: Examination of Microorganisms on Surfaces

Wed. Finish Exercises 26 and 27

Finish work on Actinomycete isolation and testing

Week of Nov. 25

Mon. Exercise 28: Identification of Bacterial Unknown – Dichotomous Keys

(Streak for isolation and perform Gram stain)

Actinomycete Lab Report Due

Wed. No Lab (Thanksgiving break)

December

Week of Dec. 2

Mon. Work on Identification of Bacterial Unknown – Dichotomous Keys

Wed. Work on Identification of Bacterial Unknown – Dichotomous Keys

Laboratory Notebook for bacterial unknown is due in lecture to Dr. Matson on Friday, Dec. 6. (This counts as the last lab report).

Week of Dec. 9

Mon. Lab Practical on Dec. 9

Wed. No Lab


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