50
Ashwin Naidu School of Natural Resources and the Environment, University of Arizona Ashwin Naidu Lindsay Smythe Ron Thompson Melanie Culver
Ashwin NaiduSchool of Natural Resources and the Environment, University of Arizona
Ashwin Naidu Lindsay Smythe Ron Thompson Melanie Culver
A brief history and current concerns
Investigating mountain lions on Kofa NWR
Mountain lion genetics study in SW AZ
Near future plan + How you can help…
Major historical radiations
-One locally distributed
-One broad ranging
Culver et al. 2000
Major restrictions to gene flow:
6 distinctsub-species
Puma concolorcouguar
Culver et al. 2000
http://www.mountainlion.org/facts_biology.a
sp
0
50
100
150
200
250
300
350
400
2000 2003 2006 2008
39W
41E
41W
44BS
45A
45B
45C
0
200
400
600
800
1000
1200
1400
1600
2000 2003 2006 2008
Total All Units
0
200
400
600
800
1000
1200
1995 1997 2000 2003 2006 2007 2008 2009
Nu
mb
er
of
Ind
ivid
ua
ls
Population Size Estimates (USFWS)
Mule deer
Bighorn sheep
Drought PredationDisease Hunting
King of Arizona mine - GOLD LOCATIONS !! (red dots)
Sex IDIndividual ID
DNA extraction
Species ID
1 354
11 Individual Mountain Lions – Captures Over Time
Population Estimation
54
51
Prey Species Identification
Prey species identification success ~ 100%
Genetic and Geographic Assessment of Population Structure, Size, Connectivity, Food Habits
Dispersal / Migration
Impact of habitat change
Source of invasions / colonisation routes
Hybridisation / introgression
Parentage / Mating systems
Level of diversity
SPECIES
SEX
INDIVIDUALS
POPULATION STRUCTURE
RELATEDNESS – KINSHIP/PARENTAGE
DIET
SOURCE POPULATIONS
MIGRATION, COLONIZATION
So…
Data is everywhere
Everyone has a piece(s)
Difficult to share
Time Consuming to collect
Non-unique Format
.ppt .doc .odp .shp .exl .txt .vsd .jpeg .tex .pdf .js
Access
Security
WILDCAT
DATABASE
To aggregate data in ‘ONE’ location –
Distributed Systems
Sharing made easier
Standardization
Formats
Access
Security
Wild Cat Database Management System
MOUNTAIN
LION
HABITATRADIOCOLLARED
POPULATIONBelongs to
Location
Sample_ type
SNP_Genotype
Indiv_IDName
Size
Hetero-zygosity
Allelic_ Diversity
Time
Collar_ ID
Coordinates
Pop_ID
Type_IDNDVI
Elevation
Mito_ Haplotype
Microsat_ Genotype
TypeOf
Home_Range
Occupies
Mountain Lion Genetic Database
WILD CAT
DATABASE
Standard Data Format
Authorized Access – Security
Method SourceNon-invasive samples?
Cost per sample
Development time
Microsatellites DNA Yes $ 1 / loc. 1 day – 3 months
Mitochondrial DNA Yes $ 5 / loc. 1 day - 1 month
Nuclear DNA sequencing
DNA Yes $ 5 / loc. 1 day – 1 month
SNP DNA Yes $ 0.01 –0.12 / SNP
2 – 3 months
100 * $100 = $10,000 Fresh samples, including labor
Morphology
Criminalistics
GeneticsPathology
Analytical Chemistry
Species Sex Kinship SourceIndividual PopulationSamples
Presentation ofEvidence
Morphology
Criminalistics
GeneticsPathology
Analytical Chemistry
Species Sex Kinship SourceIndividual PopulationSamples
Presentation ofEvidence
