| Date post: | 20-Aug-2015 |
| Category: |
Technology |
| Upload: | human-identification |
| View: | 801 times |
| Download: | 0 times |
1 Life Technologies™ |
Moving to 6-Dye Analysis: Simplifying the Process
Jennifer Schroeder, Forensic Specialist Bode Technology 10th Annual Advanced DNA Techniques Workshop San Diego, CA March 24-‐28, 2013
2 Life Technologies™ |
6-Dye Summary
Chemistry/ Standards
GeneMapper® ID-‐X So5ware
v1.4
Service
3730 DNA Analyzer /
31xx GeneEc Analyzers
3500 GeneEc Analyzer
Data CollecEon So5ware
1 2 3
31xx/3730 only All Instruments
3 Life Technologies™ |
GlobalFiler Upgrade Roadmap
Product Type 3500 3730 3130 3100
GeneMapper® ID-‐X So5ware v1.4
Data CollecEon So5ware v4.0 N/A
6-‐dye Data CollecEon Module N/A
Service Call/Computer N/A
Instrument Upgrade N/A N/A N/A
4 Life Technologies™ |
Capillary Electrophoresis Instruments
§ Supported PlaOorms
§ 3130/3130xL GeneRc Analyzer
§ 3730 DNA Analyzer
§ 3500/3500xL GeneRc Analyzer
§ Physical Upgrades needed to the instrument
§ None for any plaOorm unless you would like to upgrade a 3100 to 3130
5 Life Technologies™ |
How does your laboratory plan to comply with the requirement to generate profiles
with the new CODIS Core Loci?
A. We already have one or more 3500s, or we plan to purchase one soon
B. We have one or more 3130/3730s and we plan to enable it/them to process 6-‐dye chemistry soon
C. We would like to process 6-‐dye chemistry soon, but we haven’t decided whether to upgrade our 3130 or to replace it with a 3500
D. We’ll decide whether or not to use 6-‐dye chemistry when the requirement to use the addiEonal loci gets a liUle closer
E. We do not intend to implement 6-‐dye chemistry
6 Life Technologies™ |
Data Collection Software Requirements
§ 3130/3130xL GeneRc Analyzer
§ Data CollecRon v4
§ 6-‐Dye Data CollecRon Module
§ Life Technologies™ configured computer with MicrosoZ® Windows® 7 plaOorm
Common FAQ: Why is a new computer required? Se\ngs in both MicrosoZ® Windows® 7 plaOorm and the Data CollecRon soZware are preconfigured in the validated soZware image for the Field Service Engineer (FSE) to deploy. The computer is custom built by Dell specifically for this applicaRon and contains the exact hardware that the soZware image and the data collecRon soZware will be requiring. It is required to allow the FSE to properly deploy and service the instrument.
7 Life Technologies™ |
Data Collection Software Requirements
§ 3500/3500xL GeneRc Analyzer
§ Data CollecRon v1 or v2 § v2 requires a new computer for the same reasons described previously
§ HID Updater 3500 DC v2.0 SoZware § Installs proper run modules, required for 3500 Data CollecRon v1 or v2 to run GlobalFiler™ products
Common FAQ: If I already have a 3500 Genetic Analyzer, do I have to upgrade to Data Collection v2.0? No. Only the HID Updater CD (free) is needed to install the proper run modules. Data Collection v2.0 is available for customers who prefer the added benefits of RFID adjustments, minor updates, and the Microsoft® Windows® 7 platform.
8 Life Technologies™ |
Chemistry
§ A 6-‐dye STR PCR amplificaRon kit
§ A 6-‐dye spectral calibraRon
9 Life Technologies™ |
Software
§ GeneMapper® ID-‐X SoZware v1.4
§ Ensures proper data analysis for 6-‐dye data fragment and mixture analysis while preserving exisRng GeneMapper® ID-‐X funcRonality for analysis of 4-‐ and 5-‐dye chemistry
Common FAQ: If I already have GeneMapper® ID-‐X v1.3, can I use it for 6-‐dye analysis? While you may see 6 dyes listed in the older soZware versions, GeneMapper® ID-‐X soZware v1.2 and v1.3 were not fully developed to support 6 dye HID STR analysis and were developed prior to the GlobalFiler™ kit. Also, the panels and bins provided for GlobalFiler™ reagents for example contain the extra data for the simultaneous analysis of autosomal and Y markers that are not recognized by previous soZware versions. In addiRon to feature sets, updates to basic funcRonality specifically for 6 dye processing were needed (i.e. updates to the Analysis Method Range Filter, Peak Amplitude Thresholds, Genotypes Tables and Plot Manager, and the Mixture Analysis Tool)
10 Life Technologies™ |
Thank You
© 2013 Life Technologies CorporaRon. All rights reserved. The trademarks menRoned herein are the property of Life Technologies CorporaRon and/or its affiliate(s) or their respecRve owners. Microsoft and Windows are registered trademarks of Microsoft Corporation. Dell is a registered trademark of Dell Inc. For Forensic or Paternity Use Only The content provided herein may relate to products that have not been officially released and is subject to change without noRce.
