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Histology 6
Craig Clifford
Northeastern State University
Spring 2009
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Muscle Tissue
Responsible for movement in organs
and the body as a whole
There are three types of muscle in the
body (mesodermal origin) Skeletal
Cardiac Smooth
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Skeletal muscle
Very long cylindrical, multinucleated
cells
Quick, forceful, voluntary contractions
Cross-striations
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Cardiac muscle
Elongated, branched uninucleate cells
Cross-striations
Cells joined at intercalated disks
Involuntary, vigorous rhythmic
contractions
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Smooth muscle
Short, spindle shaped, uninucleate
cells
Slow involuntary contractions
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Special terms
In muscle cells, The cytoplasm is referred to as the
sarcoplasm The cell membrane is called the
sarcolemma
The smooth ER is know as thesarcoplasmic reticulum
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Skeletal muscle
Muscle fibers=muscle cells Very long and may be as long as the
muscle itself Multinucleated state results from the
fusion of mononucleated cells
embryonically Nuclei located peripherally, which differs
from cardiac and smooth
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Skeletal muscle
Muscle fibers contain myofibrils
Each muscle cell is surrounded by an
endomysium A bundle of muscle cells is called a fascicle
and is surrounded by a perimysium
A muscle is composed of multiple fasciclesand is surrounded by the epimysium
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Stained for laminin
NERVE
Laminin is a protein component of the endomysium.
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Tissue culture plasticware
coated without Laminin
NG-108 rat
glioma/mouse
neuroblastomacells cultured on
tissue-culture
plastic are loosely
adhered andremain rounded.
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Tissue culture plasticware
coated with Laminin
NG-108 rat
glioma/mouse
neuroblastoma cells
cultured on BDBioCoat Laminin
Cellware exhibit a
spindle-shaped
morphology anddendritic processes.
stimulate neurite outgrowth, promote cell attachment, chemotaxis,
cell differentiation and neuronal survival.
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Skeletal muscle
Note the peripheral
location of the
nuclei
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Skeletal muscle
When viewed with light transmitted
through the sections, a banding
pattern appears. A bands
I bands
Z lines H zones
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Rigor mortis
Stiffness of the body beginning 3-4hours post-mortem, peaking at 12
hours and relenting at 36 hours. Contrast:
Pallor mortis no blood flow, immediate Algor mortis decrease in temp.
1.5 F/hour
Livor mortis blood pooling (lividity) Precedes Rigor mortis
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Proprioception - Muscle
spindles
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Muscle spindles - xs
Artifact?
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Proprioception Golgi Tendon
Organ
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Proprioception Golgi Tendon
Organ
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Camillo Golgi
Born 1843
Died 1926
Physician trained inItaly
Initial studies
focused on the
nervous system
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Myoglobin stain
From what structure in the
body is this section taken?
Immunohistologically stained
for myoglobin
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Cardiac muscle
Remember, typically uninucleate (may
be binucleate), branching,
interconnecting cells.
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Cardiac muscle
The internal
arrangement of
subcellularcomponents is not
as orderly in
cardiac as opposed
to skeletal muscle.
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Cardiac muscle
Intercalated disks are areas of
connection between cells, containing
Fasciae adherentes (hemi-Z bands) Maculae adherentes (desmosomes)
Gap junctions
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Smooth muscle
Smooth muscle cells are elongated,
non-striated cells
Fusiform in shape - larger in middleand narrow at each end.
No t-tubules
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