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NationalHaemaphysalislongicornis(Asianlonghornedtick)SituationReport AsofFebruary32021

Haemaphysalislongicornis(Asianlonghornedtick)anexotic EastAsiantickhasneverpreviouslyestablishedapopulation intheUnitedStatesItisaknownseriouspestoflivestockin theAustralasianandWesternPacficRegionswhereitocurs Itisanaggresivebiterandfrequentlybuildsintense infestationsondomestichostscausinggreatstresreduced growthandproductionandseverebloodlos

Thetickcanreproduceparthenogeneticaly(withoutamale) assuchasinglefedfemaletickcancreateapopulationItis alsoaknownsuspectedvectorofseveralviralbacterialand protozoanagentsoflivestockandhumandiseasesThis three-hosttickcanspreadpathogensamongadiversehost rangeonwhichitfeedsside-by-sidewithothertickspecies Thedetectionsdetailedherearethefirstreportsofthistick outofquarantineintheUnitedStates

StateswithconfirmedlocalAsianlonghorned tickpopulationswithnumberofcountiesineach

Typeofidentification

MolecularandNVSL NVSL Molecular Taxonomic

state

Virginia WestVirginia Pennsylvania

NorthCarolina NewJersey Tennessee NewYork Kentucky Maryland

Connecticut Delaware

SouthCarolina Arkansas

Ohio RhodeIsland

32 24

12 11

10 10

7 4 4 3 2

2 1 1 1

HlongicornisidentficationNVSL=USDANationalVeterinaryServices LaboratoriesNVSLperformstaxonomicidentficationsandisthepreferedlab forconfirmationofnewstatescountiesandhostsMolecular=Confirmationof tickspeciesbypolymerasechainreaction(PCR)orotherDNA-basedtechnology Taxonomic=IdentficationbykeytickfeaturesatalabotherthanNVSL

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Asianlonghornedtickpositivehostsinformation

Numberofpositivehosts Environment

Dog White-tailedDeer 44

62 135 Typeofidentfication(single)-Notaggregated

Noidentficationprovided

Human Racoon Cow

24 22

15

MolecularandNVSL

NVSL Virginiaoposum 10

Molecular

Elk Coyote 4 Taxonomic

RedFox Groundhog

4 3 Confirmedsincelastsituationreport

Sheep 3 Easterncotontailrabbit

Goat GrayFox

2 2 2

Priorto2017tickswerecolectedfromawhite-taileddeer in2010andadogin2013

Horse 2 StripedSkunk 2 Blackbear 1

BrownBooby CanadaGoose

1 1

Chicken 1 GraySquirel 1

Greathornedowl 1 Peromyscus 1

Red-tailedhawk 1

Ca5 6

TimelineofAsianlonghornedtickhostpositives

Mar

Apr

May

Jun

Jul

Aug

Sep Oct

Nov

Dec

2017

Jan

Feb

Mar

2018 Apr

May

Jun

Jul

Aug

Sep Oct

Nov

Dec

2019

Jan

Feb

Mar

Apr

May

Jun

Jul

Aug

Sep Oct

Nov

Dec

2020

Jan

Feb

Mar

Apr

May

Jun

Jul

Aug

Sep Oct

Environment Blackbear Sheep Dog White-tailedDeer Virginiaoposum Cow Horse Racoon Goat Human Cat StripedSkunk Grayfox Easterncotontailrabbit Coyote RedFox Elk Groundhog Red-tailedhawk CanadaGoose Chicken BrownBooby GraySquirel Greathornedowl Peromyscus

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed Arkansas Benton Dog 512018 652018

Environment 452019 4102019 Connecticut Fairfield Environment 732018 8282018

Human 8212018 NewHaven Environment 692020 NewLondon Environment 9272019

Delaware Kent Environment 7262019 NewCastle Dog 6272020

Environment 6222019 Kentucky Floyd Blackbear 622017 4212020

Madison Human 7152020 Martin Elk 7152018 372019 Metcalfe Cow 5252020

Maryland Alegany Cow 642020 Environment 6122019

Howard Environment 5272020 6192020 PrinceGeorges Human 472019 532019 Washington White-tailedDeer 6252018 7262018

NewJersey Bergen BrownBooby 542020 Dog 8252017 Environment 7122018 7162018 Human 752018 7252018 RedFox 7312018

Camden Human 822019 Hunterdon Dog 6112019 7192019

Environment 5102018 682018 Racoon 532018 5252018 Sheep 812017 1192017 Virginiaoposum 532018 5252018 White-tailedDeer 4192018 4242018

Mercer Environment 5102018 642018 Middlesex Dog 962018 9262018

Environment 5102018 5142018 Goat 5242018 642018 Sheep 5172018 5232018

Monmouth Environment 872018 8172018 Pasaic Dog

Environment 10282019 11122019 Salem Environment 5272020 Somerset Cat 9282018 10262018

Dog 7312018 962018 Human 662019 792019 White-tailedDeer 5272018

Union Dog 5302013 642018 Environment 512017 4232018 Groundhog 842018 942018 Horse 512018 612018 Human 832018 Racoon 562018 VirginiaOposum 812018

Molecular NVSL MolecularandNVSL Molecular Taxonomic Taxonomic Taxonomic Taxonomic Molecular MolecularandNVSL Taxonomic MolecularandNVSL NVSL Noidentficationprovided Taxonomic NVSL NVSL NVSL NVSL Molecular NVSL Molecular Taxonomic Molecular NVSL NVSL NVSL MolecularandNVSL NVSL MolecularandNVSL MolecularandNVSL NVSL MolecularandNVSL NVSL NVSL MolecularandNVSL Noidentficationprovided NVSL Taxonomic NVSL MolecularandNVSL NVSL Molecular MolecularandNVSL MolecularandNVSL NVSL NVSL Molecular MolecularandNVSL Molecular

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed NewYork Bronx Environment 6182018

Dutchess Environment 812018 3252019 Orange Environment 5172020 Richmond Environment 632017 8142018

GraySquirel 612020 Groundhog 612020 StripedSkunk 612020 White-tailedDeer 632018

Rockland Dog 6122020 Environment 6212018 8272018 Human 6142020

Sufolk Environment 8242018 1042018 Unknown CanadaGoose 5212019 7192019 Westchester Dog 5192020

Environment 622017 Human 642018 6282018

NorthCarolina Alexander Dog 7122019Caldwell White-tailedDeer 922020Davidson Human 812018

Dog 3282019Environment 512019

Macon Cow 6302020Madison Dog 3282018Polk Virginiaopossum 9152017Rowan Elk 6102020Rutherford Dog 8142018

Cow 6202019Environment 392020

7252019 1242020 9202018

Haywood 11222019

7102020

6252018 7102020 9142018

Sury 732019

Wilkes Dog 7262019 12312019 Ohio Galia Dog 5282020 7292020 Pennsylvania Environment 872020

Human 6182019Environment 5232018Human 472020

Centre White-tailedDeer 7102018Chicken 752019Environment 532019

Dauphin Environment 4282020Delaware Environment 992019Franklin Environment 4192019Lancaster Environment 5282020

Environment 532019Human 7192019

Northampton Environment 9272019Perry Dog 5122019Philadelphia Environment 53219

Alegheny 10292020

0

Bucks 942018

7262018 Chester

1262019 10292020 1262019

10292020 Montgomery

1262019

1262019 RhodeIsland Washington Environment 6212018 SouthCarolina Lancaster Dog 8242020 12152020

Pickens Dog 8122020 9252020 Tennessee Claiborne Dog 7292019 9102019

Cocke Cow 8242019 8292019 Environment 10172019

Grainger Cat 7182020 7302020 Jeferson Dog 7192019 7222019 Knox Cow 672019 6112019

White-tailedDeer 672019 6112019 Putnam Dog 9172019 9272019 Roane Cow 5222019 5242019

Environment 6142019 Sevier Cow 8312019 9102019 Union Dog 5132019 5212019 White Dog 5152020 7102020

Taxonomic MolecularandNVSL NVSL MolecularandNVSL Taxonomic Taxonomic Taxonomic Molecular Taxonomic Molecular Taxonomic MolecularandNVSL MolecularandNVSL Noidentficationprovided Taxonomic MolecularandNVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL Taxonomic NVSL Taxonomic NVSL Molecular NVSL NVSL NVSL Taxonomic NVSL Taxonomic Taxonomic NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed Virginia Albemarle Cow 312018 5142018

Coyote 982019 Easterncotontailrabbit 782020 Environment 662018 732018 Groundhog 7192019 Human 772020 Peromyscus 7132020 Racoon 7112019 StripedSkunk 6102018 11302018 Virginiaoposum 7102019 White-tailedDeer 622018

Augusta White-tailedDeer 6232018 Botetourt Environment 1042018 Carol Dog 712018 8312018

Environment 632019 8142019 Clarke Greathornedowl 612020 8122020

RedFox 742018 Fairfax Environment 3272017 672018

Human 9202018 7182019 Fauquier Environment 852018

RedFox 572019 Floyd Environment 5262020 Frederick Grayfox 6212018 GalaxCity Environment 6212019 Giles Environment 6232018 7272018 Goochland Environment 9132019 Grayson Environment 582018 7272018 Greene RedFox 7112018 Louisa Goat 6122018 6282018 Montgomery Environment 6242019 Orange Environment 10272020 Page Cow 6252018 6292018

Red-tailedhawk 9172018 Pitsylvania Environment 652019 PrinceWiliam Environment 852019 Pulaski Cow 752018 7112018

Environment 8152019 7102020 GrayFox 6132018 9132018

Roanoke Dog 4102019 Rockbridge White-tailedDeer 5252018 9132018 Rockingham White-tailedDeer 7252018 Russel Dog 612018 8312018 Scot Dog 712018 8312018 Shenandoah Easterncotontailrabbit 6152018 Smyth Environment 672018 6282018 StauntonCity White-tailedDeer 6152018 9132018 Waren Horse 5102018 5252018 Washington Environment 5172019 Wythe Environment 6232018 7232018

NVSL Taxonomic Taxonomic NVSL Taxonomic Taxonomic Taxonomic Taxonomic NVSL Taxonomic MolecularandNVSL Molecular Molecular NVSL NVSL NVSL NVSL NVSL Taxonomic Taxonomic Taxonomic Taxonomic Molecular Taxonomic NVSL Taxonomic NVSL NVSL NVSL Taxonomic Taxonomic NVSL NVSL Taxonomic Molecular NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL Molecular NVSL NVSL NVSL NVSL NVSL

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost State County Host Earliestcolected Earliestconfirmed

WestVirginia Barbour Dog 8312018 11162018

Cabel Dog 7102018 872018

Doddridge Dog 9182019 10212019

Grant Dog 8262020 10292020

Greenbrier Environment 5272020 7142020

Hampshire White-tailedDeer 812018 512019

Cat 6212018 7102018

Cow 5182018 5212018 Hardy

Dog 6212018 7232018

White-tailedDeer 942019

Harison Environment 8192020 9162020

Kanawha Environment 4112020 4142020

Lewis White-tailedDeer 8302018 4172019

Lincoln Dog 4192018 6272018

Marion Cat 672018 762018

Mason Dog 772018 7112018

Mineral White-tailedDeer 8272019 322020

Monongalia Cat 7252019 8132019

Monroe Environment 722018 792018

Pendleton Dog 9122020 1052020

Putnam Dog 522018 6182018

Raleigh Environment 632020 7132020

cow 622019 6112019

Ritchie Dog 5262018 6192018

Environment 762018 7232018

Taylor White-tailedDeer 912017 6282018

Tyler White-tailedDeer 8312010 6252018

Upshur Coyote 6222018 8242018

Wirt White-tailedDeer 8282018 512019

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

Taxonomic

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

MolecularandNVSL

NVSL

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Bird AmericanCrow

AmericanRobin

AmericanYellowWarbler

BaldEagle

BaltimoreOriole

BarredOwl

BlackVulture

BlueJay

BrownBooby

BrownThrasher

Brown-headedCowbird

CanadaGoose

CarolinaWren

CommonGrackle

CommonYellowthroat

CoopersHawk

EasternPhoebe

EasternScreechOwl

ForstersTern

Grackle

GrayCatbird

GreatBlueHeron

Great-hornedOwl

HouseSparrow

HouseWren

IndigoBunting

NorthernCardinal

Ovenbird

Red-eyedVireo

Red-shoulderedHawk

Red-tailedHawk

SparrowUnknownSpp

SwainsonsThrush

Veery

Warbler

White-throatedSparrow

WildTurkey

WoodThrush

YellowWarbler

GrandTotal

4

4

86

1

3

1

1

3

5

2

2

3

16

3

1

4

1

15

2

2

1

2

2

2

2

13

2

3

3

1

2

1

1

4

6

7

1

212

3

1

2

20

1

1

11

39

2

2 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WasserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitat

AssociationsofHaemaphysalislongicornisTicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

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SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Mammal BlackBear

Bobcat

BrownRat

CanadaGoose

CottonRat

Coyote

DesertBigHornedSheep

DomesticDog

EasternChipmunk

EasternCottontail

EasternGraySquirrel

EasternWoodrat

Elk

Fox

GrayFox

HispidCottonRat

Hog

Human

Human(Attached)

MeadowVole

MexicanFree-tailedBat

MuleDeer

NorthernShort-tailedShrew

PeromyscusLeucopus

PeromyscusSp

Raccoon

RedDeer

RedFox

Skunk

SouthernRed-backedVole

StripedSkunk

VirginiaOpossum

White-footedMouse

White-tailedDeer

Woodchuck

WoodlandJumpingMouse

GrandTotal

2

33

1184

1

199

12

1

1

31

1

208

214

72

1

2

1

26

3

6

9

4

66

6

9

75

2

13

1

20

7

1

16

300

2527

16

21

2

39

87

14

2

1

182

2

3

1

5

1

12

2

49

11

3

37

3

3

5

1

4

6

124

16

20

2

36

74

2

3

1

5

1

12 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WaserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitatA

sociationsofHaemaphysalislongicornis

TicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

TuftsDMGoodmanLBBenedictMCDavisADVanAckerMCDiuk-WaserMA(Preprintposted2020)A

sociationoftheinvasiveHaemaphysalislongicornistickwithvertebratehosts

othernativetickvectorsandtick-bornepathogensinNewYorkCitybioRxiv20200701182626doihtpsdoiorg10110120200701182626

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Asianlonghornedtickpositivehostsinformation

Numberofpositivehosts Environment

Dog White-tailedDeer 44

62 135 Typeofidentfication(single)-Notaggregated

Noidentficationprovided

Human Racoon Cow

24 22

15

MolecularandNVSL

NVSL Virginiaoposum 10

Molecular

Elk Coyote 4 Taxonomic

RedFox Groundhog

4 3 Confirmedsincelastsituationreport

Sheep 3 Easterncotontailrabbit

Goat GrayFox

2 2 2

Priorto2017tickswerecolectedfromawhite-taileddeer in2010andadogin2013

Horse 2 StripedSkunk 2 Blackbear 1

BrownBooby CanadaGoose

1 1

Chicken 1 GraySquirel 1

Greathornedowl 1 Peromyscus 1

Red-tailedhawk 1

Ca5 6

TimelineofAsianlonghornedtickhostpositives

Mar

Apr

May

Jun

Jul

Aug

Sep Oct

Nov

Dec

2017

Jan

Feb

Mar

2018 Apr

May

Jun

Jul

Aug

Sep Oct

Nov

Dec

2019

Jan

Feb

Mar

Apr

May

Jun

Jul

Aug

Sep Oct

Nov

Dec

2020

Jan

Feb

Mar

Apr

May

Jun

Jul

Aug

Sep Oct

Environment Blackbear Sheep Dog White-tailedDeer Virginiaoposum Cow Horse Racoon Goat Human Cat StripedSkunk Grayfox Easterncotontailrabbit Coyote RedFox Elk Groundhog Red-tailedhawk CanadaGoose Chicken BrownBooby GraySquirel Greathornedowl Peromyscus

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed Arkansas Benton Dog 512018 652018

Environment 452019 4102019 Connecticut Fairfield Environment 732018 8282018

Human 8212018 NewHaven Environment 692020 NewLondon Environment 9272019

Delaware Kent Environment 7262019 NewCastle Dog 6272020

Environment 6222019 Kentucky Floyd Blackbear 622017 4212020

Madison Human 7152020 Martin Elk 7152018 372019 Metcalfe Cow 5252020

Maryland Alegany Cow 642020 Environment 6122019

Howard Environment 5272020 6192020 PrinceGeorges Human 472019 532019 Washington White-tailedDeer 6252018 7262018

NewJersey Bergen BrownBooby 542020 Dog 8252017 Environment 7122018 7162018 Human 752018 7252018 RedFox 7312018

Camden Human 822019 Hunterdon Dog 6112019 7192019

Environment 5102018 682018 Racoon 532018 5252018 Sheep 812017 1192017 Virginiaoposum 532018 5252018 White-tailedDeer 4192018 4242018

Mercer Environment 5102018 642018 Middlesex Dog 962018 9262018

Environment 5102018 5142018 Goat 5242018 642018 Sheep 5172018 5232018

Monmouth Environment 872018 8172018 Pasaic Dog

Environment 10282019 11122019 Salem Environment 5272020 Somerset Cat 9282018 10262018

Dog 7312018 962018 Human 662019 792019 White-tailedDeer 5272018

Union Dog 5302013 642018 Environment 512017 4232018 Groundhog 842018 942018 Horse 512018 612018 Human 832018 Racoon 562018 VirginiaOposum 812018

Molecular NVSL MolecularandNVSL Molecular Taxonomic Taxonomic Taxonomic Taxonomic Molecular MolecularandNVSL Taxonomic MolecularandNVSL NVSL Noidentficationprovided Taxonomic NVSL NVSL NVSL NVSL Molecular NVSL Molecular Taxonomic Molecular NVSL NVSL NVSL MolecularandNVSL NVSL MolecularandNVSL MolecularandNVSL NVSL MolecularandNVSL NVSL NVSL MolecularandNVSL Noidentficationprovided NVSL Taxonomic NVSL MolecularandNVSL NVSL Molecular MolecularandNVSL MolecularandNVSL NVSL NVSL Molecular MolecularandNVSL Molecular

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed NewYork Bronx Environment 6182018

Dutchess Environment 812018 3252019 Orange Environment 5172020 Richmond Environment 632017 8142018

GraySquirel 612020 Groundhog 612020 StripedSkunk 612020 White-tailedDeer 632018

Rockland Dog 6122020 Environment 6212018 8272018 Human 6142020

Sufolk Environment 8242018 1042018 Unknown CanadaGoose 5212019 7192019 Westchester Dog 5192020

Environment 622017 Human 642018 6282018

NorthCarolina Alexander Dog 7122019Caldwell White-tailedDeer 922020Davidson Human 812018

Dog 3282019Environment 512019

Macon Cow 6302020Madison Dog 3282018Polk Virginiaopossum 9152017Rowan Elk 6102020Rutherford Dog 8142018

Cow 6202019Environment 392020

7252019 1242020 9202018

Haywood 11222019

7102020

6252018 7102020 9142018

Sury 732019

Wilkes Dog 7262019 12312019 Ohio Galia Dog 5282020 7292020 Pennsylvania Environment 872020

Human 6182019Environment 5232018Human 472020

Centre White-tailedDeer 7102018Chicken 752019Environment 532019

Dauphin Environment 4282020Delaware Environment 992019Franklin Environment 4192019Lancaster Environment 5282020

Environment 532019Human 7192019

Northampton Environment 9272019Perry Dog 5122019Philadelphia Environment 53219

Alegheny 10292020

0

Bucks 942018

7262018 Chester

1262019 10292020 1262019

10292020 Montgomery

1262019

1262019 RhodeIsland Washington Environment 6212018 SouthCarolina Lancaster Dog 8242020 12152020

Pickens Dog 8122020 9252020 Tennessee Claiborne Dog 7292019 9102019

Cocke Cow 8242019 8292019 Environment 10172019

Grainger Cat 7182020 7302020 Jeferson Dog 7192019 7222019 Knox Cow 672019 6112019

White-tailedDeer 672019 6112019 Putnam Dog 9172019 9272019 Roane Cow 5222019 5242019

Environment 6142019 Sevier Cow 8312019 9102019 Union Dog 5132019 5212019 White Dog 5152020 7102020

Taxonomic MolecularandNVSL NVSL MolecularandNVSL Taxonomic Taxonomic Taxonomic Molecular Taxonomic Molecular Taxonomic MolecularandNVSL MolecularandNVSL Noidentficationprovided Taxonomic MolecularandNVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL Taxonomic NVSL Taxonomic NVSL Molecular NVSL NVSL NVSL Taxonomic NVSL Taxonomic Taxonomic NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed Virginia Albemarle Cow 312018 5142018

Coyote 982019 Easterncotontailrabbit 782020 Environment 662018 732018 Groundhog 7192019 Human 772020 Peromyscus 7132020 Racoon 7112019 StripedSkunk 6102018 11302018 Virginiaoposum 7102019 White-tailedDeer 622018

Augusta White-tailedDeer 6232018 Botetourt Environment 1042018 Carol Dog 712018 8312018

Environment 632019 8142019 Clarke Greathornedowl 612020 8122020

RedFox 742018 Fairfax Environment 3272017 672018

Human 9202018 7182019 Fauquier Environment 852018

RedFox 572019 Floyd Environment 5262020 Frederick Grayfox 6212018 GalaxCity Environment 6212019 Giles Environment 6232018 7272018 Goochland Environment 9132019 Grayson Environment 582018 7272018 Greene RedFox 7112018 Louisa Goat 6122018 6282018 Montgomery Environment 6242019 Orange Environment 10272020 Page Cow 6252018 6292018

Red-tailedhawk 9172018 Pitsylvania Environment 652019 PrinceWiliam Environment 852019 Pulaski Cow 752018 7112018

Environment 8152019 7102020 GrayFox 6132018 9132018

Roanoke Dog 4102019 Rockbridge White-tailedDeer 5252018 9132018 Rockingham White-tailedDeer 7252018 Russel Dog 612018 8312018 Scot Dog 712018 8312018 Shenandoah Easterncotontailrabbit 6152018 Smyth Environment 672018 6282018 StauntonCity White-tailedDeer 6152018 9132018 Waren Horse 5102018 5252018 Washington Environment 5172019 Wythe Environment 6232018 7232018

NVSL Taxonomic Taxonomic NVSL Taxonomic Taxonomic Taxonomic Taxonomic NVSL Taxonomic MolecularandNVSL Molecular Molecular NVSL NVSL NVSL NVSL NVSL Taxonomic Taxonomic Taxonomic Taxonomic Molecular Taxonomic NVSL Taxonomic NVSL NVSL NVSL Taxonomic Taxonomic NVSL NVSL Taxonomic Molecular NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL Molecular NVSL NVSL NVSL NVSL NVSL

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost State County Host Earliestcolected Earliestconfirmed

WestVirginia Barbour Dog 8312018 11162018

Cabel Dog 7102018 872018

Doddridge Dog 9182019 10212019

Grant Dog 8262020 10292020

Greenbrier Environment 5272020 7142020

Hampshire White-tailedDeer 812018 512019

Cat 6212018 7102018

Cow 5182018 5212018 Hardy

Dog 6212018 7232018

White-tailedDeer 942019

Harison Environment 8192020 9162020

Kanawha Environment 4112020 4142020

Lewis White-tailedDeer 8302018 4172019

Lincoln Dog 4192018 6272018

Marion Cat 672018 762018

Mason Dog 772018 7112018

Mineral White-tailedDeer 8272019 322020

Monongalia Cat 7252019 8132019

Monroe Environment 722018 792018

Pendleton Dog 9122020 1052020

Putnam Dog 522018 6182018

Raleigh Environment 632020 7132020

cow 622019 6112019

Ritchie Dog 5262018 6192018

Environment 762018 7232018

Taylor White-tailedDeer 912017 6282018

Tyler White-tailedDeer 8312010 6252018

Upshur Coyote 6222018 8242018

Wirt White-tailedDeer 8282018 512019

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

Taxonomic

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

MolecularandNVSL

NVSL

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Bird AmericanCrow

AmericanRobin

AmericanYellowWarbler

BaldEagle

BaltimoreOriole

BarredOwl

BlackVulture

BlueJay

BrownBooby

BrownThrasher

Brown-headedCowbird

CanadaGoose

CarolinaWren

CommonGrackle

CommonYellowthroat

CoopersHawk

EasternPhoebe

EasternScreechOwl

ForstersTern

Grackle

GrayCatbird

GreatBlueHeron

Great-hornedOwl

HouseSparrow

HouseWren

IndigoBunting

NorthernCardinal

Ovenbird

Red-eyedVireo

Red-shoulderedHawk

Red-tailedHawk

SparrowUnknownSpp

SwainsonsThrush

Veery

Warbler

White-throatedSparrow

WildTurkey

WoodThrush

YellowWarbler

GrandTotal

4

4

86

1

3

1

1

3

5

2

2

3

16

3

1

4

1

15

2

2

1

2

2

2

2

13

2

3

3

1

2

1

1

4

6

7

1

212

3

1

2

20

1

1

11

39

2

2 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WasserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitat

AssociationsofHaemaphysalislongicornisTicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

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SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Mammal BlackBear

Bobcat

BrownRat

CanadaGoose

CottonRat

Coyote

DesertBigHornedSheep

DomesticDog

EasternChipmunk

EasternCottontail

EasternGraySquirrel

EasternWoodrat

Elk

Fox

GrayFox

HispidCottonRat

Hog

Human

Human(Attached)

MeadowVole

MexicanFree-tailedBat

MuleDeer

NorthernShort-tailedShrew

PeromyscusLeucopus

PeromyscusSp

Raccoon

RedDeer

RedFox

Skunk

SouthernRed-backedVole

StripedSkunk

VirginiaOpossum

White-footedMouse

White-tailedDeer

Woodchuck

WoodlandJumpingMouse

GrandTotal

2

33

1184

1

199

12

1

1

31

1

208

214

72

1

2

1

26

3

6

9

4

66

6

9

75

2

13

1

20

7

1

16

300

2527

16

21

2

39

87

14

2

1

182

2

3

1

5

1

12

2

49

11

3

37

3

3

5

1

4

6

124

16

20

2

36

74

2

3

1

5

1

12 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WaserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitatA

sociationsofHaemaphysalislongicornis

TicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

TuftsDMGoodmanLBBenedictMCDavisADVanAckerMCDiuk-WaserMA(Preprintposted2020)A

sociationoftheinvasiveHaemaphysalislongicornistickwithvertebratehosts

othernativetickvectorsandtick-bornepathogensinNewYorkCitybioRxiv20200701182626doihtpsdoiorg10110120200701182626

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cs

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed Arkansas Benton Dog 512018 652018

Environment 452019 4102019 Connecticut Fairfield Environment 732018 8282018

Human 8212018 NewHaven Environment 692020 NewLondon Environment 9272019

Delaware Kent Environment 7262019 NewCastle Dog 6272020

Environment 6222019 Kentucky Floyd Blackbear 622017 4212020

Madison Human 7152020 Martin Elk 7152018 372019 Metcalfe Cow 5252020

Maryland Alegany Cow 642020 Environment 6122019

Howard Environment 5272020 6192020 PrinceGeorges Human 472019 532019 Washington White-tailedDeer 6252018 7262018

NewJersey Bergen BrownBooby 542020 Dog 8252017 Environment 7122018 7162018 Human 752018 7252018 RedFox 7312018

Camden Human 822019 Hunterdon Dog 6112019 7192019

Environment 5102018 682018 Racoon 532018 5252018 Sheep 812017 1192017 Virginiaoposum 532018 5252018 White-tailedDeer 4192018 4242018

Mercer Environment 5102018 642018 Middlesex Dog 962018 9262018

Environment 5102018 5142018 Goat 5242018 642018 Sheep 5172018 5232018

Monmouth Environment 872018 8172018 Pasaic Dog

Environment 10282019 11122019 Salem Environment 5272020 Somerset Cat 9282018 10262018

Dog 7312018 962018 Human 662019 792019 White-tailedDeer 5272018

Union Dog 5302013 642018 Environment 512017 4232018 Groundhog 842018 942018 Horse 512018 612018 Human 832018 Racoon 562018 VirginiaOposum 812018

Molecular NVSL MolecularandNVSL Molecular Taxonomic Taxonomic Taxonomic Taxonomic Molecular MolecularandNVSL Taxonomic MolecularandNVSL NVSL Noidentficationprovided Taxonomic NVSL NVSL NVSL NVSL Molecular NVSL Molecular Taxonomic Molecular NVSL NVSL NVSL MolecularandNVSL NVSL MolecularandNVSL MolecularandNVSL NVSL MolecularandNVSL NVSL NVSL MolecularandNVSL Noidentficationprovided NVSL Taxonomic NVSL MolecularandNVSL NVSL Molecular MolecularandNVSL MolecularandNVSL NVSL NVSL Molecular MolecularandNVSL Molecular

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed NewYork Bronx Environment 6182018

Dutchess Environment 812018 3252019 Orange Environment 5172020 Richmond Environment 632017 8142018

GraySquirel 612020 Groundhog 612020 StripedSkunk 612020 White-tailedDeer 632018

Rockland Dog 6122020 Environment 6212018 8272018 Human 6142020

Sufolk Environment 8242018 1042018 Unknown CanadaGoose 5212019 7192019 Westchester Dog 5192020

Environment 622017 Human 642018 6282018

NorthCarolina Alexander Dog 7122019Caldwell White-tailedDeer 922020Davidson Human 812018

Dog 3282019Environment 512019

Macon Cow 6302020Madison Dog 3282018Polk Virginiaopossum 9152017Rowan Elk 6102020Rutherford Dog 8142018

Cow 6202019Environment 392020

7252019 1242020 9202018

Haywood 11222019

7102020

6252018 7102020 9142018

Sury 732019

Wilkes Dog 7262019 12312019 Ohio Galia Dog 5282020 7292020 Pennsylvania Environment 872020

Human 6182019Environment 5232018Human 472020

Centre White-tailedDeer 7102018Chicken 752019Environment 532019

Dauphin Environment 4282020Delaware Environment 992019Franklin Environment 4192019Lancaster Environment 5282020

Environment 532019Human 7192019

Northampton Environment 9272019Perry Dog 5122019Philadelphia Environment 53219

Alegheny 10292020

0

Bucks 942018

7262018 Chester

1262019 10292020 1262019

10292020 Montgomery

1262019

1262019 RhodeIsland Washington Environment 6212018 SouthCarolina Lancaster Dog 8242020 12152020

Pickens Dog 8122020 9252020 Tennessee Claiborne Dog 7292019 9102019

Cocke Cow 8242019 8292019 Environment 10172019

Grainger Cat 7182020 7302020 Jeferson Dog 7192019 7222019 Knox Cow 672019 6112019

White-tailedDeer 672019 6112019 Putnam Dog 9172019 9272019 Roane Cow 5222019 5242019

Environment 6142019 Sevier Cow 8312019 9102019 Union Dog 5132019 5212019 White Dog 5152020 7102020

Taxonomic MolecularandNVSL NVSL MolecularandNVSL Taxonomic Taxonomic Taxonomic Molecular Taxonomic Molecular Taxonomic MolecularandNVSL MolecularandNVSL Noidentficationprovided Taxonomic MolecularandNVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL Taxonomic NVSL Taxonomic NVSL Molecular NVSL NVSL NVSL Taxonomic NVSL Taxonomic Taxonomic NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed Virginia Albemarle Cow 312018 5142018

Coyote 982019 Easterncotontailrabbit 782020 Environment 662018 732018 Groundhog 7192019 Human 772020 Peromyscus 7132020 Racoon 7112019 StripedSkunk 6102018 11302018 Virginiaoposum 7102019 White-tailedDeer 622018

Augusta White-tailedDeer 6232018 Botetourt Environment 1042018 Carol Dog 712018 8312018

Environment 632019 8142019 Clarke Greathornedowl 612020 8122020

RedFox 742018 Fairfax Environment 3272017 672018

Human 9202018 7182019 Fauquier Environment 852018

RedFox 572019 Floyd Environment 5262020 Frederick Grayfox 6212018 GalaxCity Environment 6212019 Giles Environment 6232018 7272018 Goochland Environment 9132019 Grayson Environment 582018 7272018 Greene RedFox 7112018 Louisa Goat 6122018 6282018 Montgomery Environment 6242019 Orange Environment 10272020 Page Cow 6252018 6292018

Red-tailedhawk 9172018 Pitsylvania Environment 652019 PrinceWiliam Environment 852019 Pulaski Cow 752018 7112018

Environment 8152019 7102020 GrayFox 6132018 9132018

Roanoke Dog 4102019 Rockbridge White-tailedDeer 5252018 9132018 Rockingham White-tailedDeer 7252018 Russel Dog 612018 8312018 Scot Dog 712018 8312018 Shenandoah Easterncotontailrabbit 6152018 Smyth Environment 672018 6282018 StauntonCity White-tailedDeer 6152018 9132018 Waren Horse 5102018 5252018 Washington Environment 5172019 Wythe Environment 6232018 7232018

NVSL Taxonomic Taxonomic NVSL Taxonomic Taxonomic Taxonomic Taxonomic NVSL Taxonomic MolecularandNVSL Molecular Molecular NVSL NVSL NVSL NVSL NVSL Taxonomic Taxonomic Taxonomic Taxonomic Molecular Taxonomic NVSL Taxonomic NVSL NVSL NVSL Taxonomic Taxonomic NVSL NVSL Taxonomic Molecular NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL Molecular NVSL NVSL NVSL NVSL NVSL

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost State County Host Earliestcolected Earliestconfirmed

WestVirginia Barbour Dog 8312018 11162018

Cabel Dog 7102018 872018

Doddridge Dog 9182019 10212019

Grant Dog 8262020 10292020

Greenbrier Environment 5272020 7142020

Hampshire White-tailedDeer 812018 512019

Cat 6212018 7102018

Cow 5182018 5212018 Hardy

Dog 6212018 7232018

White-tailedDeer 942019

Harison Environment 8192020 9162020

Kanawha Environment 4112020 4142020

Lewis White-tailedDeer 8302018 4172019

Lincoln Dog 4192018 6272018

Marion Cat 672018 762018

Mason Dog 772018 7112018

Mineral White-tailedDeer 8272019 322020

Monongalia Cat 7252019 8132019

Monroe Environment 722018 792018

Pendleton Dog 9122020 1052020

Putnam Dog 522018 6182018

Raleigh Environment 632020 7132020

cow 622019 6112019

Ritchie Dog 5262018 6192018

Environment 762018 7232018

Taylor White-tailedDeer 912017 6282018

Tyler White-tailedDeer 8312010 6252018

Upshur Coyote 6222018 8242018

Wirt White-tailedDeer 8282018 512019

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

Taxonomic

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

MolecularandNVSL

NVSL

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Bird AmericanCrow

AmericanRobin

AmericanYellowWarbler

BaldEagle

BaltimoreOriole

BarredOwl

BlackVulture

BlueJay

BrownBooby

BrownThrasher

Brown-headedCowbird

CanadaGoose

CarolinaWren

CommonGrackle

CommonYellowthroat

CoopersHawk

EasternPhoebe

EasternScreechOwl

ForstersTern

Grackle

GrayCatbird

GreatBlueHeron

Great-hornedOwl

HouseSparrow

HouseWren

IndigoBunting

NorthernCardinal

Ovenbird

Red-eyedVireo

Red-shoulderedHawk

Red-tailedHawk

SparrowUnknownSpp

SwainsonsThrush

Veery

Warbler

White-throatedSparrow

WildTurkey

WoodThrush

YellowWarbler

GrandTotal

4

4

86

1

3

1

1

3

5

2

2

3

16

3

1

4

1

15

2

2

1

2

2

2

2

13

2

3

3

1

2

1

1

4

6

7

1

212

3

1

2

20

1

1

11

39

2

2 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WasserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitat

AssociationsofHaemaphysalislongicornisTicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

ss

ss

t

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Mammal BlackBear

Bobcat

BrownRat

CanadaGoose

CottonRat

Coyote

DesertBigHornedSheep

DomesticDog

EasternChipmunk

EasternCottontail

EasternGraySquirrel

EasternWoodrat

Elk

Fox

GrayFox

HispidCottonRat

Hog

Human

Human(Attached)

MeadowVole

MexicanFree-tailedBat

MuleDeer

NorthernShort-tailedShrew

PeromyscusLeucopus

PeromyscusSp

Raccoon

RedDeer

RedFox

Skunk

SouthernRed-backedVole

StripedSkunk

VirginiaOpossum

White-footedMouse

White-tailedDeer

Woodchuck

WoodlandJumpingMouse

GrandTotal

2

33

1184

1

199

12

1

1

31

1

208

214

72

1

2

1

26

3

6

9

4

66

6

9

75

2

13

1

20

7

1

16

300

2527

16

21

2

39

87

14

2

1

182

2

3

1

5

1

12

2

49

11

3

37

3

3

5

1

4

6

124

16

20

2

36

74

2

3

1

5

1

12 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WaserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitatA

sociationsofHaemaphysalislongicornis

TicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

TuftsDMGoodmanLBBenedictMCDavisADVanAckerMCDiuk-WaserMA(Preprintposted2020)A

sociationoftheinvasiveHaemaphysalislongicornistickwithvertebratehosts

othernativetickvectorsandtick-bornepathogensinNewYorkCitybioRxiv20200701182626doihtpsdoiorg10110120200701182626

l

r

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed NewYork Bronx Environment 6182018

Dutchess Environment 812018 3252019 Orange Environment 5172020 Richmond Environment 632017 8142018

GraySquirel 612020 Groundhog 612020 StripedSkunk 612020 White-tailedDeer 632018

Rockland Dog 6122020 Environment 6212018 8272018 Human 6142020

Sufolk Environment 8242018 1042018 Unknown CanadaGoose 5212019 7192019 Westchester Dog 5192020

Environment 622017 Human 642018 6282018

NorthCarolina Alexander Dog 7122019Caldwell White-tailedDeer 922020Davidson Human 812018

Dog 3282019Environment 512019

Macon Cow 6302020Madison Dog 3282018Polk Virginiaopossum 9152017Rowan Elk 6102020Rutherford Dog 8142018

Cow 6202019Environment 392020

7252019 1242020 9202018

Haywood 11222019

7102020

6252018 7102020 9142018

Sury 732019

Wilkes Dog 7262019 12312019 Ohio Galia Dog 5282020 7292020 Pennsylvania Environment 872020

Human 6182019Environment 5232018Human 472020

Centre White-tailedDeer 7102018Chicken 752019Environment 532019

Dauphin Environment 4282020Delaware Environment 992019Franklin Environment 4192019Lancaster Environment 5282020

Environment 532019Human 7192019

Northampton Environment 9272019Perry Dog 5122019Philadelphia Environment 53219

Alegheny 10292020

0

Bucks 942018

7262018 Chester

1262019 10292020 1262019

10292020 Montgomery

1262019

1262019 RhodeIsland Washington Environment 6212018 SouthCarolina Lancaster Dog 8242020 12152020

Pickens Dog 8122020 9252020 Tennessee Claiborne Dog 7292019 9102019

Cocke Cow 8242019 8292019 Environment 10172019

Grainger Cat 7182020 7302020 Jeferson Dog 7192019 7222019 Knox Cow 672019 6112019

White-tailedDeer 672019 6112019 Putnam Dog 9172019 9272019 Roane Cow 5222019 5242019

Environment 6142019 Sevier Cow 8312019 9102019 Union Dog 5132019 5212019 White Dog 5152020 7102020

Taxonomic MolecularandNVSL NVSL MolecularandNVSL Taxonomic Taxonomic Taxonomic Molecular Taxonomic Molecular Taxonomic MolecularandNVSL MolecularandNVSL Noidentficationprovided Taxonomic MolecularandNVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL Taxonomic NVSL Taxonomic NVSL Molecular NVSL NVSL NVSL Taxonomic NVSL Taxonomic Taxonomic NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL

l

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s

rl

tl

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FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed Virginia Albemarle Cow 312018 5142018

Coyote 982019 Easterncotontailrabbit 782020 Environment 662018 732018 Groundhog 7192019 Human 772020 Peromyscus 7132020 Racoon 7112019 StripedSkunk 6102018 11302018 Virginiaoposum 7102019 White-tailedDeer 622018

Augusta White-tailedDeer 6232018 Botetourt Environment 1042018 Carol Dog 712018 8312018

Environment 632019 8142019 Clarke Greathornedowl 612020 8122020

RedFox 742018 Fairfax Environment 3272017 672018

Human 9202018 7182019 Fauquier Environment 852018

RedFox 572019 Floyd Environment 5262020 Frederick Grayfox 6212018 GalaxCity Environment 6212019 Giles Environment 6232018 7272018 Goochland Environment 9132019 Grayson Environment 582018 7272018 Greene RedFox 7112018 Louisa Goat 6122018 6282018 Montgomery Environment 6242019 Orange Environment 10272020 Page Cow 6252018 6292018

Red-tailedhawk 9172018 Pitsylvania Environment 652019 PrinceWiliam Environment 852019 Pulaski Cow 752018 7112018

Environment 8152019 7102020 GrayFox 6132018 9132018

Roanoke Dog 4102019 Rockbridge White-tailedDeer 5252018 9132018 Rockingham White-tailedDeer 7252018 Russel Dog 612018 8312018 Scot Dog 712018 8312018 Shenandoah Easterncotontailrabbit 6152018 Smyth Environment 672018 6282018 StauntonCity White-tailedDeer 6152018 9132018 Waren Horse 5102018 5252018 Washington Environment 5172019 Wythe Environment 6232018 7232018

NVSL Taxonomic Taxonomic NVSL Taxonomic Taxonomic Taxonomic Taxonomic NVSL Taxonomic MolecularandNVSL Molecular Molecular NVSL NVSL NVSL NVSL NVSL Taxonomic Taxonomic Taxonomic Taxonomic Molecular Taxonomic NVSL Taxonomic NVSL NVSL NVSL Taxonomic Taxonomic NVSL NVSL Taxonomic Molecular NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL Molecular NVSL NVSL NVSL NVSL NVSL

l

l

r

FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost State County Host Earliestcolected Earliestconfirmed

WestVirginia Barbour Dog 8312018 11162018

Cabel Dog 7102018 872018

Doddridge Dog 9182019 10212019

Grant Dog 8262020 10292020

Greenbrier Environment 5272020 7142020

Hampshire White-tailedDeer 812018 512019

Cat 6212018 7102018

Cow 5182018 5212018 Hardy

Dog 6212018 7232018

White-tailedDeer 942019

Harison Environment 8192020 9162020

Kanawha Environment 4112020 4142020

Lewis White-tailedDeer 8302018 4172019

Lincoln Dog 4192018 6272018

Marion Cat 672018 762018

Mason Dog 772018 7112018

Mineral White-tailedDeer 8272019 322020

Monongalia Cat 7252019 8132019

Monroe Environment 722018 792018

Pendleton Dog 9122020 1052020

Putnam Dog 522018 6182018

Raleigh Environment 632020 7132020

cow 622019 6112019

Ritchie Dog 5262018 6192018

Environment 762018 7232018

Taylor White-tailedDeer 912017 6282018

Tyler White-tailedDeer 8312010 6252018

Upshur Coyote 6222018 8242018

Wirt White-tailedDeer 8282018 512019

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

Taxonomic

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

MolecularandNVSL

NVSL

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Bird AmericanCrow

AmericanRobin

AmericanYellowWarbler

BaldEagle

BaltimoreOriole

BarredOwl

BlackVulture

BlueJay

BrownBooby

BrownThrasher

Brown-headedCowbird

CanadaGoose

CarolinaWren

CommonGrackle

CommonYellowthroat

CoopersHawk

EasternPhoebe

EasternScreechOwl

ForstersTern

Grackle

GrayCatbird

GreatBlueHeron

Great-hornedOwl

HouseSparrow

HouseWren

IndigoBunting

NorthernCardinal

Ovenbird

Red-eyedVireo

Red-shoulderedHawk

Red-tailedHawk

SparrowUnknownSpp

SwainsonsThrush

Veery

Warbler

White-throatedSparrow

WildTurkey

WoodThrush

YellowWarbler

GrandTotal

4

4

86

1

3

1

1

3

5

2

2

3

16

3

1

4

1

15

2

2

1

2

2

2

2

13

2

3

3

1

2

1

1

4

6

7

1

212

3

1

2

20

1

1

11

39

2

2 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WasserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitat

AssociationsofHaemaphysalislongicornisTicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

ss

ss

t

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Mammal BlackBear

Bobcat

BrownRat

CanadaGoose

CottonRat

Coyote

DesertBigHornedSheep

DomesticDog

EasternChipmunk

EasternCottontail

EasternGraySquirrel

EasternWoodrat

Elk

Fox

GrayFox

HispidCottonRat

Hog

Human

Human(Attached)

MeadowVole

MexicanFree-tailedBat

MuleDeer

NorthernShort-tailedShrew

PeromyscusLeucopus

PeromyscusSp

Raccoon

RedDeer

RedFox

Skunk

SouthernRed-backedVole

StripedSkunk

VirginiaOpossum

White-footedMouse

White-tailedDeer

Woodchuck

WoodlandJumpingMouse

GrandTotal

2

33

1184

1

199

12

1

1

31

1

208

214

72

1

2

1

26

3

6

9

4

66

6

9

75

2

13

1

20

7

1

16

300

2527

16

21

2

39

87

14

2

1

182

2

3

1

5

1

12

2

49

11

3

37

3

3

5

1

4

6

124

16

20

2

36

74

2

3

1

5

1

12 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WaserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitatA

sociationsofHaemaphysalislongicornis

TicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

TuftsDMGoodmanLBBenedictMCDavisADVanAckerMCDiuk-WaserMA(Preprintposted2020)A

sociationoftheinvasiveHaemaphysalislongicornistickwithvertebratehosts

othernativetickvectorsandtick-bornepathogensinNewYorkCitybioRxiv20200701182626doihtpsdoiorg10110120200701182626

l

t

c

s

rl

tl

lt

t

r

FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost

State County Host Earliestcolected Earliestconfirmed Virginia Albemarle Cow 312018 5142018

Coyote 982019 Easterncotontailrabbit 782020 Environment 662018 732018 Groundhog 7192019 Human 772020 Peromyscus 7132020 Racoon 7112019 StripedSkunk 6102018 11302018 Virginiaoposum 7102019 White-tailedDeer 622018

Augusta White-tailedDeer 6232018 Botetourt Environment 1042018 Carol Dog 712018 8312018

Environment 632019 8142019 Clarke Greathornedowl 612020 8122020

RedFox 742018 Fairfax Environment 3272017 672018

Human 9202018 7182019 Fauquier Environment 852018

RedFox 572019 Floyd Environment 5262020 Frederick Grayfox 6212018 GalaxCity Environment 6212019 Giles Environment 6232018 7272018 Goochland Environment 9132019 Grayson Environment 582018 7272018 Greene RedFox 7112018 Louisa Goat 6122018 6282018 Montgomery Environment 6242019 Orange Environment 10272020 Page Cow 6252018 6292018

Red-tailedhawk 9172018 Pitsylvania Environment 652019 PrinceWiliam Environment 852019 Pulaski Cow 752018 7112018

Environment 8152019 7102020 GrayFox 6132018 9132018

Roanoke Dog 4102019 Rockbridge White-tailedDeer 5252018 9132018 Rockingham White-tailedDeer 7252018 Russel Dog 612018 8312018 Scot Dog 712018 8312018 Shenandoah Easterncotontailrabbit 6152018 Smyth Environment 672018 6282018 StauntonCity White-tailedDeer 6152018 9132018 Waren Horse 5102018 5252018 Washington Environment 5172019 Wythe Environment 6232018 7232018

NVSL Taxonomic Taxonomic NVSL Taxonomic Taxonomic Taxonomic Taxonomic NVSL Taxonomic MolecularandNVSL Molecular Molecular NVSL NVSL NVSL NVSL NVSL Taxonomic Taxonomic Taxonomic Taxonomic Molecular Taxonomic NVSL Taxonomic NVSL NVSL NVSL Taxonomic Taxonomic NVSL NVSL Taxonomic Molecular NVSL NVSL NVSL Taxonomic NVSL NVSL NVSL NVSL Molecular NVSL NVSL NVSL NVSL NVSL

l

l

r

FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost State County Host Earliestcolected Earliestconfirmed

WestVirginia Barbour Dog 8312018 11162018

Cabel Dog 7102018 872018

Doddridge Dog 9182019 10212019

Grant Dog 8262020 10292020

Greenbrier Environment 5272020 7142020

Hampshire White-tailedDeer 812018 512019

Cat 6212018 7102018

Cow 5182018 5212018 Hardy

Dog 6212018 7232018

White-tailedDeer 942019

Harison Environment 8192020 9162020

Kanawha Environment 4112020 4142020

Lewis White-tailedDeer 8302018 4172019

Lincoln Dog 4192018 6272018

Marion Cat 672018 762018

Mason Dog 772018 7112018

Mineral White-tailedDeer 8272019 322020

Monongalia Cat 7252019 8132019

Monroe Environment 722018 792018

Pendleton Dog 9122020 1052020

Putnam Dog 522018 6182018

Raleigh Environment 632020 7132020

cow 622019 6112019

Ritchie Dog 5262018 6192018

Environment 762018 7232018

Taylor White-tailedDeer 912017 6282018

Tyler White-tailedDeer 8312010 6252018

Upshur Coyote 6222018 8242018

Wirt White-tailedDeer 8282018 512019

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

Taxonomic

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

MolecularandNVSL

NVSL

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Bird AmericanCrow

AmericanRobin

AmericanYellowWarbler

BaldEagle

BaltimoreOriole

BarredOwl

BlackVulture

BlueJay

BrownBooby

BrownThrasher

Brown-headedCowbird

CanadaGoose

CarolinaWren

CommonGrackle

CommonYellowthroat

CoopersHawk

EasternPhoebe

EasternScreechOwl

ForstersTern

Grackle

GrayCatbird

GreatBlueHeron

Great-hornedOwl

HouseSparrow

HouseWren

IndigoBunting

NorthernCardinal

Ovenbird

Red-eyedVireo

Red-shoulderedHawk

Red-tailedHawk

SparrowUnknownSpp

SwainsonsThrush

Veery

Warbler

White-throatedSparrow

WildTurkey

WoodThrush

YellowWarbler

GrandTotal

4

4

86

1

3

1

1

3

5

2

2

3

16

3

1

4

1

15

2

2

1

2

2

2

2

13

2

3

3

1

2

1

1

4

6

7

1

212

3

1

2

20

1

1

11

39

2

2 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WasserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitat

AssociationsofHaemaphysalislongicornisTicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

ss

ss

t

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Mammal BlackBear

Bobcat

BrownRat

CanadaGoose

CottonRat

Coyote

DesertBigHornedSheep

DomesticDog

EasternChipmunk

EasternCottontail

EasternGraySquirrel

EasternWoodrat

Elk

Fox

GrayFox

HispidCottonRat

Hog

Human

Human(Attached)

MeadowVole

MexicanFree-tailedBat

MuleDeer

NorthernShort-tailedShrew

PeromyscusLeucopus

PeromyscusSp

Raccoon

RedDeer

RedFox

Skunk

SouthernRed-backedVole

StripedSkunk

VirginiaOpossum

White-footedMouse

White-tailedDeer

Woodchuck

WoodlandJumpingMouse

GrandTotal

2

33

1184

1

199

12

1

1

31

1

208

214

72

1

2

1

26

3

6

9

4

66

6

9

75

2

13

1

20

7

1

16

300

2527

16

21

2

39

87

14

2

1

182

2

3

1

5

1

12

2

49

11

3

37

3

3

5

1

4

6

124

16

20

2

36

74

2

3

1

5

1

12 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WaserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitatA

sociationsofHaemaphysalislongicornis

TicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

TuftsDMGoodmanLBBenedictMCDavisADVanAckerMCDiuk-WaserMA(Preprintposted2020)A

sociationoftheinvasiveHaemaphysalislongicornistickwithvertebratehosts

othernativetickvectorsandtick-bornepathogensinNewYorkCitybioRxiv20200701182626doihtpsdoiorg10110120200701182626

l

l

r

FirstdateofAsianlonghornedtickdetectionsbystatecountyandhost State County Host Earliestcolected Earliestconfirmed

WestVirginia Barbour Dog 8312018 11162018

Cabel Dog 7102018 872018

Doddridge Dog 9182019 10212019

Grant Dog 8262020 10292020

Greenbrier Environment 5272020 7142020

Hampshire White-tailedDeer 812018 512019

Cat 6212018 7102018

Cow 5182018 5212018 Hardy

Dog 6212018 7232018

White-tailedDeer 942019

Harison Environment 8192020 9162020

Kanawha Environment 4112020 4142020

Lewis White-tailedDeer 8302018 4172019

Lincoln Dog 4192018 6272018

Marion Cat 672018 762018

Mason Dog 772018 7112018

Mineral White-tailedDeer 8272019 322020

Monongalia Cat 7252019 8132019

Monroe Environment 722018 792018

Pendleton Dog 9122020 1052020

Putnam Dog 522018 6182018

Raleigh Environment 632020 7132020

cow 622019 6112019

Ritchie Dog 5262018 6192018

Environment 762018 7232018

Taylor White-tailedDeer 912017 6282018

Tyler White-tailedDeer 8312010 6252018

Upshur Coyote 6222018 8242018

Wirt White-tailedDeer 8282018 512019

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

Taxonomic

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

NVSL

MolecularandNVSL

NVSL

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Bird AmericanCrow

AmericanRobin

AmericanYellowWarbler

BaldEagle

BaltimoreOriole

BarredOwl

BlackVulture

BlueJay

BrownBooby

BrownThrasher

Brown-headedCowbird

CanadaGoose

CarolinaWren

CommonGrackle

CommonYellowthroat

CoopersHawk

EasternPhoebe

EasternScreechOwl

ForstersTern

Grackle

GrayCatbird

GreatBlueHeron

Great-hornedOwl

HouseSparrow

HouseWren

IndigoBunting

NorthernCardinal

Ovenbird

Red-eyedVireo

Red-shoulderedHawk

Red-tailedHawk

SparrowUnknownSpp

SwainsonsThrush

Veery

Warbler

White-throatedSparrow

WildTurkey

WoodThrush

YellowWarbler

GrandTotal

4

4

86

1

3

1

1

3

5

2

2

3

16

3

1

4

1

15

2

2

1

2

2

2

2

13

2

3

3

1

2

1

1

4

6

7

1

212

3

1

2

20

1

1

11

39

2

2 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WasserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitat

AssociationsofHaemaphysalislongicornisTicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

ss

ss

t

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Mammal BlackBear

Bobcat

BrownRat

CanadaGoose

CottonRat

Coyote

DesertBigHornedSheep

DomesticDog

EasternChipmunk

EasternCottontail

EasternGraySquirrel

EasternWoodrat

Elk

Fox

GrayFox

HispidCottonRat

Hog

Human

Human(Attached)

MeadowVole

MexicanFree-tailedBat

MuleDeer

NorthernShort-tailedShrew

PeromyscusLeucopus

PeromyscusSp

Raccoon

RedDeer

RedFox

Skunk

SouthernRed-backedVole

StripedSkunk

VirginiaOpossum

White-footedMouse

White-tailedDeer

Woodchuck

WoodlandJumpingMouse

GrandTotal

2

33

1184

1

199

12

1

1

31

1

208

214

72

1

2

1

26

3

6

9

4

66

6

9

75

2

13

1

20

7

1

16

300

2527

16

21

2

39

87

14

2

1

182

2

3

1

5

1

12

2

49

11

3

37

3

3

5

1

4

6

124

16

20

2

36

74

2

3

1

5

1

12 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WaserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitatA

sociationsofHaemaphysalislongicornis

TicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

TuftsDMGoodmanLBBenedictMCDavisADVanAckerMCDiuk-WaserMA(Preprintposted2020)A

sociationoftheinvasiveHaemaphysalislongicornistickwithvertebratehosts

othernativetickvectorsandtick-bornepathogensinNewYorkCitybioRxiv20200701182626doihtpsdoiorg10110120200701182626

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020 USDAWildlifeServices

Bird AmericanCrow

AmericanRobin

AmericanYellowWarbler

BaldEagle

BaltimoreOriole

BarredOwl

BlackVulture

BlueJay

BrownBooby

BrownThrasher

Brown-headedCowbird

CanadaGoose

CarolinaWren

CommonGrackle

CommonYellowthroat

CoopersHawk

EasternPhoebe

EasternScreechOwl

ForstersTern

Grackle

GrayCatbird

GreatBlueHeron

Great-hornedOwl

HouseSparrow

HouseWren

IndigoBunting

NorthernCardinal

Ovenbird

Red-eyedVireo

Red-shoulderedHawk

Red-tailedHawk

SparrowUnknownSpp

SwainsonsThrush

Veery

Warbler

White-throatedSparrow

WildTurkey

WoodThrush

YellowWarbler

GrandTotal

4

4

86

1

3

1

1

3

5

2

2

3

16

3

1

4

1

15

2

2

1

2

2

2

2

13

2

3

3

1

2

1

1

4

6

7

1

212

3

1

2

20

1

1

11

39

2

2 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WasserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitat

AssociationsofHaemaphysalislongicornisTicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

ss

ss

t

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Mammal BlackBear

Bobcat

BrownRat

CanadaGoose

CottonRat

Coyote

DesertBigHornedSheep

DomesticDog

EasternChipmunk

EasternCottontail

EasternGraySquirrel

EasternWoodrat

Elk

Fox

GrayFox

HispidCottonRat

Hog

Human

Human(Attached)

MeadowVole

MexicanFree-tailedBat

MuleDeer

NorthernShort-tailedShrew

PeromyscusLeucopus

PeromyscusSp

Raccoon

RedDeer

RedFox

Skunk

SouthernRed-backedVole

StripedSkunk

VirginiaOpossum

White-footedMouse

White-tailedDeer

Woodchuck

WoodlandJumpingMouse

GrandTotal

2

33

1184

1

199

12

1

1

31

1

208

214

72

1

2

1

26

3

6

9

4

66

6

9

75

2

13

1

20

7

1

16

300

2527

16

21

2

39

87

14

2

1

182

2

3

1

5

1

12

2

49

11

3

37

3

3

5

1

4

6

124

16

20

2

36

74

2

3

1

5

1

12 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WaserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitatA

sociationsofHaemaphysalislongicornis

TicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

TuftsDMGoodmanLBBenedictMCDavisADVanAckerMCDiuk-WaserMA(Preprintposted2020)A

sociationoftheinvasiveHaemaphysalislongicornistickwithvertebratehosts

othernativetickvectorsandtick-bornepathogensinNewYorkCitybioRxiv20200701182626doihtpsdoiorg10110120200701182626

ss

ss

t

SummaryofwildlifesamplingforAsianlonghornedtick

Animalgroups Host

Numberofanimals

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Numberconfirmed

SCWDS Tuftsetal2019amp2020USDAWildlifeServices

Mammal BlackBear

Bobcat

BrownRat

CanadaGoose

CottonRat

Coyote

DesertBigHornedSheep

DomesticDog

EasternChipmunk

EasternCottontail

EasternGraySquirrel

EasternWoodrat

Elk

Fox

GrayFox

HispidCottonRat

Hog

Human

Human(Attached)

MeadowVole

MexicanFree-tailedBat

MuleDeer

NorthernShort-tailedShrew

PeromyscusLeucopus

PeromyscusSp

Raccoon

RedDeer

RedFox

Skunk

SouthernRed-backedVole

StripedSkunk

VirginiaOpossum

White-footedMouse

White-tailedDeer

Woodchuck

WoodlandJumpingMouse

GrandTotal

2

33

1184

1

199

12

1

1

31

1

208

214

72

1

2

1

26

3

6

9

4

66

6

9

75

2

13

1

20

7

1

16

300

2527

16

21

2

39

87

14

2

1

182

2

3

1

5

1

12

2

49

11

3

37

3

3

5

1

4

6

124

16

20

2

36

74

2

3

1

5

1

12 TuftsDMVanAckerMCFernandezMPDeNicolaAEgiziAampDiuk-WaserMA(2019)DistributionHost-SeekingPhenologyandHostandHabitatA

sociationsofHaemaphysalislongicornis

TicksStatenIslandNewYorkUSAEmergingInfectiousDiseases25(4)792-796

TuftsDMGoodmanLBBenedictMCDavisADVanAckerMCDiuk-WaserMA(Preprintposted2020)A

sociationoftheinvasiveHaemaphysalislongicornistickwithvertebratehosts

othernativetickvectorsandtick-bornepathogensinNewYorkCitybioRxiv20200701182626doihtpsdoiorg10110120200701182626