National reports -overview of regional

status

Regional Workshop 2: Improving Capacity for Diagnosis of Fish and Molluscan Diseases

Banja Luka, Bosnia and Herzegovina, 20-24 October 2013, Hotel Palas

Production data 2012

Most represented – carp and rainbow troutLess – other cyprinid and salmonid speciesand marine fish (Croatia, Montenegro, BiH)MolluscsMusselsOysters

Movement/transfers Between farms within country Importation of eggs/fry/live fishImportation of market sized fish for human

consumptionImportation of fry for cultivation from

neighboring countries and European countries (Hungary, Italy, Bulgaria etc)

Importation of eggs mainly from Denmark and USA

Diseases recorded in the countries

Viral diseases – IPNV in Croatia, Bosnia and Herzegovina, Serbia

VHSV – single outbreak in CroatiaBacterial diseases – very similar in all countries

– furunculosis, yersiniosis, BKD, flavobacteriosis, carp erithrodermatitis, columnaris disease, motile aeromonas septicaemia (MAS)

Marine fish species – vibriosis, tenacibaculosisParasitic diseases – marteiliosis in mussels in

Croatia and Montenegro; ectoparasites

Fungal diseases – saprolegniosis

Other health problems – Newly described diseases

RMS /strawberry disease

Diagnostic capacity in the countries

Aquatic animal diseases laboratory within country

All coutries some kind of NRL with different responsibilities; research and educational institution dealing with aquatic animal health

Some are mostly educational and other research

Level of diagnostic

Level of diagnostic:Level 1: Gross observation, general

necropsy (external, internal)Level II: Bacteriology, Parasitology,

Mycology, HistologyLevel III: Cell lines, Immunological tests,

TEM, Molecular techniquesMOST COUNTRIES – LEVEL II

Analysis of diagnstic capacity

List of diseases and diagnostic methods used List is more or less same VHS, IPN, SVC, KHV – clinical observation,

detection of gross changes, histological observation, isolation of virus on cell culture, identification by ELISA, PCR,

Furunculosis, yersiniosis, BKD, carp erythrodermatitis, columnaris – Isolation of bacteria on bacteriological media, identification of genus by selective media and general properties, identification of the species by API or any other kit for biochemical identification

Surveillance of nationally listed diseases

Diagnostic methods – VHS, IHNV, PVŠ, IPNV, KHV, BKD – according to the level of lab capacity

Sampling – organised by Competent Authority

Results – presence of IPNV, VHSV, PVŠ not diagnosed in last two years, BKD sporadically

Surveillance and monitoring of other pathogens

Targeted pathogens and aquaculture species –

Croatia – bacteria V. anguillarum and Tenacibaculum maritimum in sea bass, Yersinia ruckeri and Flavobacterium psyhrophilum – antimicrobial resistance

Eustrongyloides sp. – possible human health threat – no programme of control until now

RMS, MAS, ECV – regionally present - due to insufficient funding no surveillance or monitoring programmes

Study of abnormal mortality

Notification of abnormal mortalities in Macedonia, available diagnostic methods performed – mortality caused by abiotic factors

Similar cases in fish farms/ open waters are notified in other counties -

Conclusions and perspectives

Decrease of aquaculture production in BiHImproving progress in harmonisation of

legislative in Serbia, MontenegroUnsufficent laboratory capacity in BiH,

Montenegro and Macedonia (they are not complying with OIE/EU standards for diagnosis of listed viral diseases)

There are no diagnostic methods for crustacean diseases developed in any country in the region

Future needs

Education for insufficient laboratory methodsImprovement of lab equipment and

proceduresEvaluation of diagnostic possibilities through

interlaboratory proficiency testing (maybe it is possible to organise it regionally within this project)

Implementation of diagnostic methods for other species (crustacean)

Regional networking