Neuromics_Presentation_V4

8/7/2019 Neuromics_Presentation_V4

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Tools and CapabilitiesTools and CapabilitiesFocus on cell based assaysFocus on cell based assays

and gene expression analysisand gene expression analysis

for early phase Drug Discoveryfor early phase Drug Discovery

3/23/2011-Version 4


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Focus: Provide researchers a subset of tools and methods to enable successful

outcomes in early phase drug discovery. Failure is success when

determined early.**90% of lead candidates identified by current in vitro systems fail to become drugs

Value (simplified): Physiologically relevant primary human (STEMEZ), rat and mouse

cells

Better in vitro screening of targets and molecules = Reduced animal model use

Shorten development timeline

Lowerdownstream costs

Images: STEMEZ hNP1 can be

differentiated into various neuronal

subtypes underdefined conditions.

DAPIG AP Antibody

> 80% Tuj1+

> 60% M P2+

T3


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Slide 2

T3 Prefer not to reference the BG02 cells. These cells are no longer available for use in NIH funded research and we do not use them to

manufacture STEMEZ cellsTWS, 2/23/2011


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Why Neuromics?Standing out in a crowded room

We have a experience serving many large Pharmas, Biotechs,

cademic and overnment Labs.

Customersuccess focused Expert technical support

etailed protocols and methods

Quick replacements and reorders

Large expert resource network

Published expert input

Customerfeedback and data sharing throughsocial media outlets and blogs


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Testimonial: got 10 million cells total afterextraction from the tissue. t ay 4 they all developed long axons.Thank you so much for the replacement." Dr. Lidia Gardner, University of Tennessee HSC..

Gene Expression

nalysis

Media/3-

Gels/ECMS

poptosis

etection

Cell Migration

ssays

Growth

Factors/Markers pplications include:

Proliferation and

neurogenesis

Neuronal differentiation

Neurite outgrowthCell migration

Neurotoxicity screening

Cell based compound

screening

G-protein coupled receptors

Ion channels

Stem cell transplantation

Derived andmanufactured

neural cells fromhuman

embryonic stemcell line H9

(WA09).

Stable, Potent and Cost Effective Cells


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Markers/ ntibodies Growth Factors/Culture Media Gene Expression nalysis poptosis etection

Imges: GFAP (Catalog#:

aCH22102) Staining of of

Astrocytes in the Ventral

horn.Courtesy ofDr. Qi-lin

L. Cao, University of

Louisville School of

Medicine. Rat spinal cordhomogenate WBs: NF-H

,

NF-M,, NF-L,, NF66and

GFAP.

STEMEZTMhNP1 Human

Neural Progenitors treatedwith hGDNF cultured in

STEMEZ hNP1 Neural

ProgenitorExpansion

Media

i-Fect knockdown of Delta Opioid

Receptor. Molecular Pain 2005,

1:29

Jurkat cells dually

stained with Hoechst

and Polycaspase

ssay Kit,

Assay kits, Qualified Media and Reagents-Sample

Images


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Cell Based Assays-Examples

STEMEZhN2 Human Neural Discovery Kit

Phenotyping

Electrophysical and FLIPRData Provided upon request

Neural phenotypes derived from N2 cell

lines. ( ) Phase contrast image of

differentiated culture. (B) Network

including post-mitotic motoneurons

(HB9). (C) Cholinergic neuron. (D) Tuj-1

positive cells that are DAT-positive

(dopamine transporter; closed arrow)

and DAT-negative (open arrow). (E)

Gabaergic neurons, inset illustrates

GABA in axon, but not the dendrites

(arrow).

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Tools and Capabilities


STEMEZ hNP1 Human Neural Progenitor Kit is an efficient tool to quantitatively

detect both stimulators and inhibitors of cell migration when used with the OrisTM Cell

Migration Assay Kit (Platypus Technologies). Method development to date indicates that

the assay has the potential foradaptation as a homogenous HTS-suitable cell-based

assay.


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Primary Rat Neurons and Astrocytes

Transfection of functional HeV

glycoproteins and infection with HeV

pseudotyped virions.In orderto establish

the feasibility of carrying out the proposed

experiments in primary neurons, we show

(figure ) that ourassays are amenable to

use in primary neurons. In the experiment, Combined Hippocampus, Cortex, and Ventricular-E18

(Neuromics) were plated, and at 3 days were transfected with plasmids encoding HeV G/F as well

as YFP. On the following day, these cells were infected with HeV orVSV pseudotyped viruses

bearing RFP. In the figure, (A) the phase contrast photos show the differentiated neurons; (B)

upon excitation forRFP, the red fluorescence indicates neurons infected by HeV pseudotyped

virions; (C) upon excitation forYFP, and the green fluorescence shows the efficiency of

transfection in neurons. This experiment indicates that the proposed experiments can be carried

out in primary neurons, which are transfectable and infectable in oursystems, and thus supports

all the proposed aims. Data Courtesy ofDr. Matteo Porotto, WeillCornell MedicalCollege. Larger

Image

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Primary Rat Neurons and Astrocytes

Data Courtesy of Evanna Gleason, Louisiana State University

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Gene Expression Analysis Its all about delivery!

i-FectTM siRNA delivery kit : Success in delivering siRNA to

DOR, hTERT, The 3 subunit of the Na+,K+-ATPase,

rSNSR1, NTS1. NAV1.8, Survivin, TRL4, Flaviviruses and

more.

Tools fordelivering plasmids, proteins, antisense and

othersmall RNA and DNAmolecule.

Transfection Delivery: CustomerPublications


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More Key Product Category Links:

Antibodies-Markers

Apoptosis Research Reagents -includes:

detection kits, antibodies and proteins

Proteins Recombinant and natural proteins-

includes a wide selection of cell growth factors.

Transfection Reagents-Gene ExpressionAnalysis

Cell Growth Media


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ClearUnderstanding of where Neuromics could havea positive impact. More intense in vitro screening of compounds and

targets.

Bettercandidates fordownstream processes.

Propose solutions based on this understanding

Follow up, tweak and improve

3/23/2011


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Pete Shuster

CEO and Owner

Direct Phone: 612-801-1007

[email protected]

Social Networking:

www.neuromics.com

www.neuromics.net

neuromics.blogspot.com

sirnatransfection.blogspot.com

3/23/2011

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