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Neuroscience for Kids

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    Neuroscience For Kids

    Successful Science Fair Projects

    By Lynne Bleeker(Science teacher, science fair organizer and judge)

    A successful science fair project does not have to be expensive or even terribly time-consuming. However, it does require some planning and careful thought. Projects becomefrustrating to students, parents and teachers when they are left to the last minute and thusdon't have the chance to be as good as they possibly can. You can't rush good science!

    A Science Fair Project display usually asks that you include certain sections. Your particularscience fair rules and guidelines may use slightly different words to describe them, but be sureyou address each of them as you go through your project and then again as you write it up.

    Sections of a Science Fair ProjectTitle

    Ideally the title of your project should be catchy, an "interest-grabber," but it should alsodescribe the project well enough that people reading your report can quickly figure out what

    you were studying. You will want to write your Title and Background sections AFTER you havecome up with a good question to study.

    Background or Purpose

    The background section is where you include information that you already know about yoursubject and/or you tell your project readers why you chose the project you did. What were youhoping to find out from the project?

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    The Question (Or Selecting Your Subject)

    Probably the most difficult part of a science fair project is coming up with a good subject toresearch. I suggest to my students that they:

    A. think about WHAT INTERESTS them.B. think of a TESTABLE QUESTION about the subject.

    If you are doing a project about something that interests you, you will likely enjoy the researchmore and stick with it long enough to get some good data. Remember, you are being a

    scientist. Scientists go to work each day because they are interested in whatthey are studying and because they are curious to know the answers to the questions they areresearching.

    If you are working to ANSWER A QUESTION, you will be doing real research. (Often studentstell me that their parents have suggested doing something such as "volcanoes" or "tornadoes."It is possible to build cute models of these things, but it is pretty hard to come up with questionsabout them that are testable with materials available to the average person and in the timeframe between when the science fair project is assigned and when it is due!) Another problemoccurs when students need special equipment to test a question. For example, it might beinteresting to find out if television commercials really are louder than regular programming ...but how would you test that without a decible-meter?

    Some of the best science fair projects I have seen have also been the simplest. For example, Ihad students whose parents bought "off-brands" of cereal. They wondered if those brandswere really any different from the name brands. They bought 3 or 4 different brands of thesame type of cereal and asked permission to test them with the whole class. They had their

    peers evaluate them for taste, appearance, and sogginess in milk after 1 minute. They also dida cost comparison. They got a lot of interesting data! (I won't tell you what they found out incase you want to do something similar!) Other students who like sports have done experimentswith the equipment for their sport: Do new tennis balls bounce higher than old ones? Dobasketballs that are fully inflated bounce better than flatter ones? These projects just requiresome tennis balls or basketballs, some volunteer "bouncers" and a meter tape or meter stick!

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    There are many good sources for science fair project questions. The Neuroscience For KidsWeb Site has some neuroscience-related questions that might spark your interest. Projectsinvolving food - tasting, smelling etc - can be very simple to set up yet also very interesting."Can blindfolded people taste the difference between ...?" You can also get lots of ideas fromscience trade books, such as Janice Van Cleave's books ("Biology for Every Kid" etc). If youbrowse through these books at a store or library, they may give you some ideas for a project ofyour own.

    Project Guidelines

    Be sure to carefully read the project guidelines for your particular science fair. Rules varygreatly from fair to fair in what is allowed, both for safety and ethical/animal use considerations.Obviously, experiments should not involve illegal substances or involve clearly preventabledanger to you or your research subjects.

    Some situations may require clarification from your teacher and/or parents. For example,suppose you were doing an experiment on the effects of caffeine (or chocolate) onconcentration or reflexes. Think about the possible consequences! You would need to getpermission before providing large amounts of high-caffeine soda pop. Some science fairsdiscourage the use of food in experiments because of food allergies. Again, check with yourscience fair guidebook or your teacher, and be sure you clearly communicate to your (human)research subjects what you will be asking them to consume so they can tell you if they haveallergies.

    Some science fairs allow experiments with live animals and others don't. For example, oneclassic experiment (found in most older science fair project books) involves changing thetemperature of fish tank water and seeing what it does to the respiration rate (breathing) ofgoldfish. In some places around the country, that experiment would not be allowed at all. In

    others, you would need a special permission form guaranteeing that you will take good care ofthe animal. In other places, they don't yet have such restrictions. Again, use common sense. Ionce had students do a very clever experiment to see if their hamster or their cat could learn togo through a maze more quickly. This experiment, though it had animal subjects, obviouslyinvolved no chance of harming the cat or the hamster so I gave them permission to do it.Generally you are safest if your experiments involve plants or insects, and both types oforganisms can lead to some fascinating studies! If you REALLY want to do an experiment withyour pet, be prepared to explain what information you are hoping to gain from the experimentand how you will ensure the safety of the animal.

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    Prediction or Hypothesis

    As soon as you come up with a testable question, you will probably instantly have a hypothesis(prediction) about what the results will be from your testing. (Isn't the human brain an amazingthing?!) It's a good idea to write this down before starting, because it may change as you goabout your experimenting.

    Materials and Methods

    Once you have come up with a question that you can actually test with materials at yourdisposal, you need to figure out how to set up the tests. If you will have a survey for yourparticipants to fill out, get that written up and duplicated. If you will need a chart to write downyour test results, get it made. If you take the time to make it look nice with a straight-edge, youcan include the actual chart or survey instrument in your project write-up. This really impressesthe judges!

    Let your teacher or science fair coordinator know what your question is and how you plan to goabout testing it. They will likely have some good suggestions to save you lots of time andtrouble. Once you have their go-ahead, then make a list of your materials, gather them up andGET STARTED! If you are really doing science, you will probably find that some things don't goquite as you had predicted they would. You will have to modify your research methods or evenyour original question. You may have to add more materials to your list. My students often getdiscouraged by this, but actually it is a good thing. This is how science really works!

    Keep good notes of the things you have tried and plan to include even the "didn't-works" and"mess-ups" in your project report. Be sure to try your experiment several times to be sure youhave enough data to make a logical conclusion. If you tell me that one brand of cereal getssoggier in milk but you've only tried each cereal in one cup of milk, I would suspect that maybeit was a fluke; you need lots of "trials" (generally at least 3; the more, the better) for believabledata. Remember, too, that you want to keep all of the experimental factors (variables) the

    same except the one you are testing. In the cereal experiment, it wouldn't be fair to all of thecereals if you left one brand in milk for one minute and tried the others after two minutes orsomething like that. Again, GET STARTED EARLY on carrying out your project. You can't still

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    be doing the experiment the day before the project is due and expect to have a first-class write-

    up!

    In science fair projects as in life, "a picture is worth a thousand words." Plan to take pictures ofthe materials you used and of the experiment as it is being carried out. If you get started early,you will have time to have the pictures developed and include them as part of your report. (Or ifyou are lucky and your school has cameras that will take pictures and put them right into thecomputer, you will have time to learn how to do that and print them out for your report.)

    Results or Data

    The results section is where you tell your reader the actual numbers (or other data) that yougot as you were doing the experiment. (In the tennis ball experiment, this would be a table withthe different brands of balls and the actual heights each of them bounced on each trial.) Youmight also include a graph, if your data lends itself to it. But you do not tell your interpretation ofthe data - that's for the last section.

    Conclusion

    In the conclusion you finally get to tell your readers what you found out from the experiment, orhow you interpret your data. Students often like to use this section to expand upon how muchthey liked doing the experiment (and how wise the teacher was to require such a goodassignment!) or how much they learned from it ... but really this section should be focused onwhat you learned about your original question and hypothesis. For example, DID cheapercereals get soggier in milk faster?

    The Display

    Project displays tend to be another source of great frustration to students, teachers andparents ... but they don't have to be! Again, what you need to do is PLAN AHEAD and then

    THINK OF YOUR AUDIENCE. Remember that they weren't there when you did theexperiment, so what seems obvious to you will not be obvious to them unless you make itextremely clear.

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    Check to see if your science fair has any special rules to guide your display. For example, arethere rules about the size of your display? Ideally, choose a display board that is cardboardand a "tri-fold," meaning that it folds into a middle and two side sections. This shape is themost stable and will stand up in the science fair display. These boards can be ordered fromsupply companies and are also usually available at stores like Office Depot. Check and see ifyour school has some from last year that can be re-used. This is good for the environment andfor your pocketbook! I strongly advise against the flimsier posterboard, which tends to fall downeasily and irritates teachers and judges. Also avoid wood backboards, which are VERY difficult

    to transport!

    Once you have written or typed up all of the above sections, be sure you have TITLES for eachsection that are large and legible (I'd suggest 24 point or so on the computer). That way ifpeople have questions about some part of your project, they can go right to the section theyneed to answer their question. Arrange the sections of the report on the board in a way that isattractive and also logical. The purpose and hypothesis should be easy to see right away. Anart teacher can give you some good suggestions about how to use paper of different colors todraw attention to parts of the report and make it look terrific!

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    HOW TO BUILD SIMPLE TELEGRAPH SETS.

    W1TP TELEGRAPH & SCIENTIFIC INSTRUMENT MUSEUMS:

    http://w1tp.com

    Prof. Tom Perera - W 1 T P - Historian and Collector

    ClickBACK to

    return to

    the main

    Telegraph

    Web

    Page.

    HOW TOBUILD SIMPLE TELEGRAPH SETS.

    The Electric Telegraph is one of the most important inventions in the history of science ! Itdirectly led the way to the development of all digital communications including computers,fax, the internet, email, and text messaging.

    I am often asked for help in building a working electric telegraph set for use in schoolprojects, science fairs, displays and demonstrations.

    I have tried to outline the process of building a simple telegraph set in the paragraphs below

    and I have included several variations as well as a simple wireless telegraph set.

    Before you start to build a telegraph set, I STRONGLY SUGGEST that you read HOW ATELEGRAPH SET WORKS.This LINK describes the THEORY and OPERATION of the "electric telegraph".(4KB)

    BUILDING A WORKING TELEGRAPH SYSTEMMany people have inquired about how to build a simple telegraph system to approximate the

    early systems used in the 19th century. You can view thousands of pictures of telegraph sets in my

    main telegraph museum:http://w1tp.com

    Here is a photo of one homemade key and sounder that was build to look just like some ofthe telegraph sets that were used from 1844 to the 1950s:

    http://w1tp.com/pertel.htmhttp://w1tp.com/pertel.htmhttp://w1tp.com/http://w1tp.com/http://w1tp.com/http://w1tp.com/http://w1tp.com/pertel.htm
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    As you can see, this was a carefully thought-out and executed project.

    Many peoplejust want tobuild a verysimple set tobecome familiarwith the basic

    principles of theelectrictelegraph.

    The followingproject is thesimplest functional telegraph system construction project that I could design. It requiresvery few parts and all of them should be commonly available.

    The project uses readilyavailable parts:

    PARTS LIST:

    2 Pieces of wood. (Any kind of wood will do fine.)

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    9 Small wood screws or nails.

    2 Large IRON nails.(About 2-3 inches long.)

    (NOTE: It is important that these be IRON or STEEL nails. Aluminum and copper nails willnot work.)

    (You can test these nails to be sure that they are Iron or Steel by making sure that a

    magnet is attracted to them or by holding a magnetic compass against them to see if it

    deflects the compass needle.)

    4 Flat strips of bendable metal.

    Three of them should be about 4 inches long.

    One should be about 7 inches long.

    The long one MUST be iron-bearing or so-called "ferrous" metal which is metal that is

    attracted by a magnet.

    (This kind of metal is often found in some food cans.)

    (Be careful not to cut yourself as you cut the strips of metal !)

    (You can test this metal strip to be sure that it is ferrous by making sure that a magnet is

    attracted to it or by holding a magnetic compass against it to see if it deflects the compass

    needle.

    20 ft or more ofINSULATED solid electrical wire.

    (22 - 30 gauge.... (the metal part of the wire should be about 1/64 inch or less in

    diameter.)

    (Radio Shack sells appropriate wire as part number: 278-1345, 278-1215, or 278-502 for

    about $4.00 )

    (The MORE turns of wire you can wind around the nail, the stronger its magnetism will be

    and the better it will work.)

    2 Flashlight batteries. (The "D" Cells shown work best.)

    CONSTRUCTION:

    Construction of the telegraph set is very simple. Just look at the photographs and you will see how

    it is put together.

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    BE CAREFUL NOT TO CUT YOURSELF ON THE EDGES OF THE METALSTRIPS.

    If children will be using the set, you will want to round all sharp corners with a file orsandpaper and perhaps put tape over any exposed sharp edges.

    The key is made byscrewing one of the stripsof metal to one of thepieces of wood so thatpushing down on the stripbrings the strip intoelectrical contact with thescrew that is mountedunder it.

    The Battery Holder: is madeby screwing two of the metalstrips to the wood so thatthey can make electrical

    contact with each end of thelineup of the two batteries. Arubber-band may be used tomaintain pressure on thebattery contacts.,br>

    Be sure to put the batteries inthe holder with the positivetip of one battery pushing against the negative bottom of the other battery. This is called a"series connection" of the batteries and adds the 1.5 volt voltage of one battery to the 1.5volt voltage of the other battery to produce a total of 3 volts. Most flashlights are designed

    this way.

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    The Sounder: requires a bit of care inconstruction and adjustment.

    The electromagnet coil consists of one of theiron nails with at least 100 turns of the wire

    wound neatly around it. ( If possible wind on200 or more turns to make the magnetic forcestronger. )

    The longer iron-bearing strip of metal isscrewed to the wooden base and bent so thatit extends up and over the top of the nail.This piece has been labeled "IRON-BEARING" in the parts photograph to indicate that it is pulled in by the magnet. Manyfood cans are made of this type of metal. Be careful not to cut yourself on any sharp edges.

    When the electric current passes through the coil of wire, it makes the nail into anelectromagnet which pulls the strip of metal down to the nail and makes a clicking sound.(You may have to carefully adjust the strip of metal so it is close enough to the nail allow itto be pulled down by the magnet.)

    The second nail is important because it keeps the strip of metal from pulling too far awayfrom the electromagnet. It also serves to make a clicking sound when the strip of metal isreleased by the magnet and moves upward.

    To complete the telegraph set, simply connect the key, batteries, and sounder with the wiresas shown in the first picture.

    SAFETY NOTE:When the coil is deenergized by opening the telegraph key, some stored electricity isreleased.It is possible to receive an electric shock if you are touching both of the contacts of the keywith your fingers while you release the key.(This can not happen if you build the telegraph key as shown in the pictures because thebottom contact can not be touched while pressing the key down.)

    There are two ways to eliminate this problem:

    1. Make it impossible to touch the lower contact of the key.The key in the pictures has a lower contact that is covered by the top contact and it is notpossible to touch it at the same time that the top of the key is being pressed.

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    2. Place a diode between the two contacts on the key. Radio Shack sells a diode that willabsorb the electricity when the key is released. The Radio Shack part numbers forappropriate diodes are: 276-1101, 276-1102, 276-1102 or 276-1104 and they cost about $1.00.Just connect the two wires of the diode to the two contacts on the telegraph key with the

    white band on the diode being connected to the side of the key that is connected to thepositive (+) side of the battery. If you have connected the diode incorrectly, opening thekey will not open the circuit. In that case, simply reverse the diode connections.

    OPERATION OF THE TELEGRAPH SET:

    When you push down on the telegraph key it completes the electrical circuit from the key to one

    end of the coil and from the other end of the coil to one end of the battery and from the otherterminal of the battery back to the other side of the key.

    Now the sounder magnet should pull down the metal strip and make a CLICKING sound. When you

    release the key, the metal strip should spring upwards and make a CLACKING sound.

    (If it does not work, please see the "Troublshooting section below.)

    You can learn to tell the difference between the dots and the dashes of the Morse code bylearning to tell the difference between the pull-in "CLICK" and the release-"CLACK".

    The pull-in "CLICK" is the sound the metal strip makes when it is pulled in by theelectromagnet coil and strikes the nail which is in the center of the coil.

    The release "CLACK" is the sound that the metal strip makes when it is no longer pulled bythe electromagnet coil and it moves rapidly upward to strike the upper nail.

    The Morse Code Characters are called DOTS (or 'dits') and DASHES (or 'dahs') They aremade from the CLICKS and CLACKS as follows:

    A DOT is created when there is just a LITTLE time between the pull-in CLICK and therelease CLACK.Try it now: Push the key and quickly release it.Did you hear the CLICK followed quickly by the CLACK ?

    That was a DOT in Morse code.

    A DASH is created when there is a LONGER time between the pull-in CLICK and therelease CLACK.Try it now: Push the key and after a short wait, release it.Did you hear the CLICK followed after a short wait by the CLACK ?That was a DASH in Morse code.

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    Now let's send the letter "A".In Morse Code, the letter "A" is: DOT DASH. (or Dit Dah).Push down the key and release it quickly to make a DOT and then push down the key, waita bit, and release it to make a DASH.Try it! You have just sent the Morse Code letter "A".

    Now let's send your name in Morse Code:Find the letters in your name in this Morse Code table that shows the dot and dashequivalents of letters and numbers in the American Morse Code and the International code:http://w1tp.com/percode.htm(The American Morse Code was mostly used by the railroads and the International codewas used for wireless and radiotelegraphy and in foreign lands.)

    TELEGRAPH SETS BUILT BY OTHER PEOPLE:

    Here is another version of this set:It was made by 13 year-old Claire Berry in KwaZulu-Natal, South Africa. It won a highgrade in a science project.

    http://w1tp.com/percode.htmhttp://w1tp.com/percode.htmhttp://w1tp.com/percode.htm
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    Here is another version of this set:

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    This is a closeup view of the sounder:

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    Here is a third version of this set:

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    Here is an excellent Telegraph Science Project by 5th. Grader Katie:

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    Here are three students practicing with their telegraph set at a school in England:

    NOTES:

    You may use up to 100 feet of wire between the key and the sounder.

    Please be careful not to hurt yourself while building this set. The improper use of toolsduring construction can cause serious injury. The original designer of the set and themaintainer of this web page accept no liability for injuries caused by the construction oroperation of the set.

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    Here is the circuit diagram of the completed telegraph set:The components are all in what is called a 'series circuit'. When the key is closed, theelectricity flows in 'series' from the batteries through the closed key to the sounder andthrough the sounder coil back to the other terminal on the batteries.(The 2 batteries are combined

    !-----KEY-----!

    ! !

    ! BATTERY 1

    SOUNDER BATTERY 2

    ! !

    !-------------!

    And Here is one of the circuits which will allow two of these sets

    to be hooked together to send messages to a distant friend. You may

    use up to about 100 feet of wire to connect to the other set:

    This is the circuit which was used in the early land-line telegraph

    stations. Please NOTE that ONE KEY must be in the 'closed' positionin order for the other key to operate the sounders. Most of the

    early keys had a built-in shorting switch to keep the key circuit

    closed and make it possible to receive messages. One advantage of

    this circuit is that the battery can be at either end of the wire

    or even in the middle.

    Wires to other set:

    !------KEY------X.......BATTERY........X-----KEY-----!

    ! !

    SOUNDER SOUNDER

    ! SET 1. Set 2. !

    !---------------X......................X-------------!

    Wires to other set:

    *** NOTE: ONE key MUST be kept CLOSED in order for the other to work. ***

    Here is another circuit which was designed by Bill Horne, W1AC.

    The advantage of this circuit is that it allows either key

    to operate the sounders at any time without wearing down the batteries

    and

    without the need to close the circuit closing switches on the keys.

    NOTE: For this circuit to work, It IS IMPORTANT to wire the batteries

    with

    the POSITIVE (+) and NEGATIVE (-) Terminals as shown.

    If you do not do this, the sounders will close as soonas they are connected.

    NOTE: It is also IMPORTANT to use the same voltage..

    ( ..for example, 2 D-cell batteries.. ) for each battery.

    Wires to other set:

    !----SOUNDER----!......................!----SOUNDER----!

    + ! + ! ! + ! +

    BATTERY KEY KEY BATTERY

    - ! - SET 1. ! ! Set 2. - ! -

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    !---------------!......................!---------------!

    Wires to other set:

    *** BE SURE THE (+) and (-) BATTERY TERMINALS ARE WIRED AS SHOWN ***

    TROUBLESHOOTING PROBLEMS AND SOLUTIONS

    Overheating and dead batteries {CAUSED BY USING UNINSULATED WIRES) One parent emailed that all his completed set did was to have the coil of wire get very hotand that the batteries went dead shortly afterwards.

    After asking him a few questions, I found out that he had wound bare (uninsulated) wirearound the nail. I explained that the coil of wire had to be made from insulated wire so thatthe individual turns in the coil did not electrically connect to each other.

    Sticking Sounder Won't release quickly or at allOne parent noted that closing the key caused the electromagnet to attract the metal armitureof the sounder but that it did not release after the circuit was opened.

    I suggested increasing the strength of the springiness of the metal that pulled the armitureaway from the nail........ ANDINSERTING a very thin piece of plastic sheeting or wrapping materal between the nail and

    the armiture to prevent the metal armiture from directly coming into contact with the nail.

    Can't tell the differece between a CLICK and a CLACK

    Try using a different metal nail as the upper 'stop'.

    Try putting a bit of aluminum foil on the upper stop to change the sound of the release CLACK.

    MODIFICATIONS AND ADDITIONS:

    There are many ways that you can modify this basic circuit.

    For instance, you could put a flashlight bulb or a 3-volt sounder or buzzer in the seriescircuit so it would flash or buzz in addition to clicking when you close the key.This might help some people to learn to copy the Morse Code more easily.

    You could also mount a pencil on the sounder arm and have it mark a piece of paper witheither a high mark or a low mark while the paper was pulled under the pencil at an

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    approximately constant speed. This would work exactly like Samual Morse's very firsttelegraph systems that were used before people learned to copy the Morse Code by ear.Morse invented what he called a "register" which used a clockwork mechanism to pull apaper tape under a pencil which was moved in and out by an electromagnet.

    If you use this system it is quite easy to translate the short or long downwards pencil markson the paper into dots and dashes. Registers were used to make Morse Code marks on papertape right up to the time of the American Civil war when people finally discvered theycould copy Morse Code by ear. President Abraham Lincoln had a telegraph office that kepthim informed of the progress of the Civil War by receiving telegraph messages from hisgenerals in the fields of battle.

    Good Luck with your project...

    HOW THE TELEGRAPH WORKS:

    Explanation of how the telegraph works:

    BUILDING A WORKING 'WIRELESS' TELEGRAPH SET:

    Please click on the following link if you would like to build:

    A simple working wireless telegraph set:(15KB)

    A CIRCUIT FOR OPERATING AN OLD SOUNDER FROM AUDIO TONES:

    If you already have an old telegraph sounder and would like to have it operate in response to

    audio tones such as those from a code practice oscillator or short wave receiver, you will find a

    simple circuit diagram at this link:

    A simple circuit for driving a telegraph sounder from audio tones:(20KB)

    ----->> ADDITIONS, CORRECTIONS, and COMMENTS ARE WELCOME ! ! ! ! !

    Click BACK to return to the main Telegraph Web Page.

    Professor Tom PereraMontclair State University

    Email Address:

    (I receive over 200 spam messages daily.

    To help me avoid them, I ask you to type my email address as follows with no spaces

    between words:)

    http://w1tp.com/pertel.htmhttp://w1tp.com/pertel.htmhttp://w1tp.com/perwirls.htmhttp://w1tp.com/perwirls.htmhttp://w1tp.com/3010.gifhttp://w1tp.com/3010.gifhttp://w1tp.com/3010.gifhttp://w1tp.com/perwirls.htmhttp://w1tp.com/pertel.htm
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    PLEASE TYPE: keys

    THEN TYPE THE @ SYMBOL

    THEN TYPE: w1tp.com

    Please NOTE: It is w1tp Not: wLtp or wItp.

    Please NOTE: You MUST include the word KEYS in the Email Subject Line.

    ( Please Inquire Before Sending Attachments Larger Than 100KB ! )

    Internet On-Line Telegraph & Scientific Instrument Museum:

    http://w1tp.com

    or:

    http://www.chss.montclair.edu/~pererat/telegrap.htm

    Internet ENIGMA Museum:http://w1tp.com/enigma

    http://w1tp.com/enigmahttp://w1tp.com/enigmahttp://w1tp.com/enigmahttp://w1tp.com/enigma
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    Renal Function Science Fair ProjectThe Effects of Different Liquids on Renal Function?

    CATEGORY: Life Science

    STUDENT: Jessica

    GRADE LEVEL: 9th (High School)

    STATE / Country: California, USA

    AWARDS:1st Prize Winner of the 2006 Virtural Summer Science Fair Contest - Super Science Fair Projects

    Problem Being Investigated: Which types of fluids affect the kidneys in different ways? What is the pH of the

    urine excreted? I will research this problem and conduct tests to answer these questions.

    To see Jessica'sRenal Function Science Fair Research Report ... look here....

    Abstract - Renal Function Science Fair Project

    Three types of liquid were ingested (isotonic saline, tap water, and coffee) over five twenty minute intervals to

    determine how much urine each beverage would produce, and the pH of each of the urine from these fluids. Of

    the three liquids, coffee indeed did produce the most urine.

    I. Purpose

    The purpose of my Renal Function Science Fair Project experiment is to show the kidneys aptitude to modify

    the production of urine in response to the ingestion of different liquids, such as tap water, isotonic saline (whichis Gatorade or any other sports drink), and coffee. Various fluids affect the kidneys in different ways. This

    experiment is useful in everyday life because as I ponder on the busy, routines much of us complete daily, it

    would be helpful to know which fluids cause us to have to use the restroom more frequently than others. Also,

    it will be useful to know if certain fluids can be hazardous to our health by causing dehydration.

    II. Procedure

    The controls of the Renal Function Science Fair Project experiment are as follows: The participant must not

    consume large amounts of liquid at any meal preceding the experiment. This is to empty the bladder in order

    not to alter the results. The participant should also not consume drinks (other than the coffee) that contain

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    theophylline (tea). The participant must control the amount of perspiration and condensation from the body

    (Also not to alter the results of experimentation). The independent variables of this experiment are the three

    different types of liquid being consumed. The dependent variables are how much urine that is collected at each

    interval.

    1. Purchase and collect the necessary materials:

    (a) 1 Liter of Gatorade or any other generic sports drink that contains isotonic saline.

    (b) 1 Liter of Tap Water

    (c) 1 liter of regular coffee

    (d) 1 500 Milliliter beaker

    (e) Strips of pH paper

    (f) Drinking cups

    (g) Timer

    (h) Journal to record results from each experimentation

    2. Ingest one liter of tap water rapidly to minimize the total time required for ingestion.

    3. Record the time at completion of ingestion4. Collect urine samples at 20 minute intervals for a total of five twenty minute periods. Each collection

    and total volume of each should represent a complete emptying of the bladder.5. Record the total volume collected and the color of the urine.6. Test the urine by carefully holding and placing the pH paper in the beaker.7. Record the urines pH.8. After every measurement and color is recorded, discard the urine.

    These procedures are to be repeated with each different liquid ingested.

    III. ConclusionThrough experimentation, I have discovered that the tap water, isotonic saline, and coffeeconsumed indeed affected the kidneys in different ways. In the beginning of the experiment

    with the tap water, the color of the urine had a much darker coloration. As the experimentprogressed, the urine became more dilute and less concentrated. The pH of the urine heldsteady, but fluctuated slightly from 6 to 8.The elimination of substantial amounts of urine is dueto two principal factors. When the tap water was ingested, there was an increase in bloodvolume, which increases blood pressure and the kidneys rate of filtration. This caused agreater output of urine. The increase in blood volume is due mainly to the increase in water.This lowers the osmotic pressure of blood which in turn inhibits the levels of secretion ofantidiuretic hormones. A reduction in the level of this hormone reduces water re-absorptionthus increasing the output of urine. I was able to collect an average volume of 143.06 ML ofurine with the ingestion of the tap water.

    With the isotonic saline I saw a result that was similar to that of the tap water. The color of theurine also started out dark in this experiment, but began to lighten towards the end. The pH ofthe urine did not fluctuate highly, but remained between 6 and 7. The effect the isotonic salinehad on the kidneys was much like that of the tap water. The saline in the drink causes thekidneys rate of filtration to increase, as well as the blood volume. The osmotic pressure of theblood changes very little because of the effects of the saline. Through experimentation, I wasable to collect an average excretion volume of 152.53 ML urine.

    Towards the beginning of experimentation with the coffee I noticed the same dark coloration ofurine as observed in the two other liquids. With time, the urine changed to a pale yellow-green.Some of the dark coloration could have been attributed to the concentration of the urine, but it

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    also may have been because of the pigments in the coffee as certain colors in fluid or foodhave the ability to alter the urines color. The caffeine in coffee dilates the afferent arteriole tothe kidney and greatly enhances the rate of filtration. The net result is an increase in the rate ofurine formation. The pH varied slightly from a 5.5 to a 6. The coffee produced a large volume ofurine. I have discovered that it produced an average excretion of 293 ML.

    Here is what I would do different: This year I have learned much about science fair projectsand what is required to complete one, but I have learned a few things that I would like to use inthe future. First, next year I would like to include more controls and variables in my project. Forexample, on the project that I did this year, I realized that another possible control could be theamount of water that is released from my body in perspiration, or exhaled breath. This controlcould possibly have affected the amount of urine that I collected to a minor extent, but it wouldstill be important if I was to continue my project in the future.

    In order to prove a scientific experiment, continued testing must be priority. If I was to do this

    project again or to continue it, I would like to spend more time testing my project (9 months to ayear rather than six months) and using other liquids as well as the ones included in myexperiment. Thanks Again! Jessica

    Renal Function Science Fair Project Bibliography

    Ahlstrom, Timothy P. The Kidney Patient's Book. Delran, New Jersey: Great Issues Press, 1991.

    Inlander, Charles B. The Consumer's Medical Desk Reference. New York: Stone Song Pres,Inc.,1995. pp 289, 327, 441, 28-30.

    Silverstein, Dr. Alvin. The Excretory System. New York: Twenty First Century Books, 1994

    The Human Body: The Kidneys: Balancing the Fluids. New York:Torstar Books, Inc., 1985.

    Wimer, L.T. Animal Physiology Lab Studies

    Zhang, Z. Kindu, C. Yuan.,J Ward, E. Lee. De Mayo, H. Westphal, A. Mukherjee., "SevereFiberonectin Deposit Renal Glomecular Disease in Mice Lacking Uterglobin.", Science 30 May,1997:1408-1412

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    More display boards and abstracts will be added like the science fair projects abstracts-1. (Iadd another page as students submit them. So please subscribe to my free monthlynewsletter,Science Fair Enthusiasts, to stay up to date.)...

    What do you get when you try four different kinds of popcorn to seewhich one pops the most?

    A Fun 2nd grade science project!

    Shriek wanted to participate in the science fair this year. The Umpirehelped her. They worked really hard and ended up with a great scienceproject that they had a lot of fun with. I think they also had some reallyfun daddy daughter time.

    What they did was choose four different kinds of pop corn

    Jiffy Pop

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    Microwave Bag

    kernals on the stove

    Microwave Bucket

    They popped them all for the same amount time and counted thekernals that did not pop and the popped corn to see which one was thewinner. Shriek told me she had a lot of fun putting the kernals in littlegroups of ten to count them. I say any time you can make math fun gofor it!

    I am not going to tell you the results in case someone else wants to usethis idea for their childs project. because it really was a funsimple science project idea.

    Shriek loved learning how popcorn pops and had a lot of fun putting herposter together. I am really glad she participated, even if my house stillsmelled of burnt popcorn for several days after the finished the project

    * I am also over atMommy Momenttoday talking about rewardcharts. I like them but they never last too long at my house. I wouldlove some tips!

    CHEMICAL CONTAMINATION

    OF LAKES AND STREAMS

    One way to test for contamination in water from lakes, rivers and streams is with a bioassay.

    A bioassay uses a living organism--usually a plant or a bacteria--as a test agent for the presence or

    concentration of a chemical compound or adisease. The idea is to choose a test agent that is very

    sensitive to the condition you are testing.

    Have you ever read about how miners took canaries down into mines to act as early warnings of

    gas leaks? Because canaries are more sensitive to gas than people, the birds reacted to very small

    amounts of gas and gave miners a chance to escape. You could say canaries were a bioassay for

    underground gas.

    Different plants are often used as bioassays because they respond in a predictable way and are

    often very sensitive to the condition that is being tested. A standard toxic dose--the level at which

    no seeds of the bioassay plant sprout or all the plants die--is established as a reference point. Then

    samples are tested and compared to the reference standard.

    Of all the possible water-quality bioassay organisms, lettuce might be one of the last you would

    think of. Lettuce doesn't live in water, so why use it to test water quality? The reason is lettuce

    bioassays are inexpensive, easy to do, and the seeds are pretty sensitive to some types of

    contaminants in water, including heavy metals, pesticides and other organic toxins. Although any

    variety of lettuce may do, Lactuca sativa Buttercrunch is the standard variety recommended for

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    bioassays by the U.S. Environmental Protection Agency, the Food and Drug Administration, and

    the Organization for Economic Cooperation and Development.

    You might try taking a series of samples along one stream or compare streams near industry to

    water running though agricultural areas.

    Directions for conducting experiments can be found at: Lettuce Bioassay.

    (http://ei.cornell.edu/toxicology/bioassays/lettuce/)

    ACID RAIN

    For most of the following experiments, you will need a pH indicator, such as wide-range litmus or

    pH paper, a garden soil pH testing kit, or a pH indicator that you can make yourself in Experiment

    3. These pH indicators contain a chemical that changes color when it comes in contact with acids

    or bases. For example, litmus and pH paper turn red in strong acids and blue in strong bases.

    Because only a few pH indicators measure pH over a wide range of pH values, you will need to find

    out the pH range of the indicator you use. Typically, the color chart provided with each pH

    indicator kit will show the pH range of that indicator. Color pH indicators provide only an

    approximate measure of the pH, or the strength of the acid or base. They are not as accurate as

    the expensive instruments scientists use to measure pH, but they are adequate for the following

    experiments.

    Measuring With pH Paper

    When measuring pH with pH paper, dip the end of a strip of pH paper into each mixture you want

    to test. After about two seconds, remove the paper, and immediately compare the color at the

    wet end of the paper with the color chart provided with that pH indicator. Write down the pH

    value and color. Always use a clean, unused strip of pH paper for each mixture that you test.

    Measuring Liquids with a Garden Soil pH Test Kit

    Soil pH test its are designed to measure the pH of soil, but they may also be used to measure the

    pH of liquids, such as water and water mixtures. Most of these kits contain a test solution (liquid

    pH indicator), color chart, and clear plastic test container, such as a test tube.

    To measure pH, pour 1/4 teaspoon of the mixture you want to test into the test container, and

    add 1/4 teaspoon of the test solution provided in the kit. Cover the container and shake once or

    twice to mix, or stir if necessary. Compare with the color chart provided with the kit and write

    down the result.

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    Tips

    - Except for pHtest paper, all the materials called for in these experiments, including distilled

    water and borax, can be obtained at grocery stores or from local lawn and garden stores or

    nurseries.

    - pH test paper can beordered online.

    - Inexpensive garden soil pH testing kits are available at most lawn and garden stores or nurseries.

    These testing kits usually contain a pH indicator solution that covers a range of at least pH 4 to 10,

    which is wide enough for most of the following experiments.

    - You may substitute baking soda for household ammonia in the experiments. If you do, be sure to

    stir well because baking soda does not dissolve easily in water unless heated. The pH of

    undissolved baking soda will not be the same as dissolved baking soda.

    - You may substitute fresh-squeezed lemon juice for white vinegar. Lemon juice is slightly more

    acidic than the vinegar sold in grocery stores. White vinegar is preferred over cider vinegar or

    lemon juice because it is colorless and relatively free of impurities.

    - Use clean, dry containers and utensils.

    Experiment 1: Measuring pH

    This experiment will illustrate how to measure the approximate pH of chemicals in water using a

    pH indicator. A pH indicator is a chemical that changes color when it comes in contact with acids

    or bases.

    Materials

    - pH paper and color chart (pH range 3 to 12) or garden soil pH testing kit distilled water (available

    at grocery stores and drug stores)

    - white vinegar

    - household ammonia (or baking soda)

    - 3 small, clear cups or glasses

    - 3 stirring spoons

    - measuring cups and spoons (1/2 cup, 1/4 and 1 teaspoon)

    - notebook and pencil

    Instructions

    - Rinse each cup with distilled water, shake out excess water, and label one cup vinegar, the

    second cup ammonia, and the third cup water.

    - Pour 1/2 cup distilled water into each of the 3 cups.

    - Add 1/2 teaspoon white vinegar to the vinegar cup and stir with a clean spoon.- Add 1/2 teaspoon ammonia to the ammonia cup and stir with a clean spoon.

    - Do not add anything to the water cup.

    - Dip an unused, clean strip of pH paper in the vinegar cup for about 2 seconds and immediately

    compare with the color chart. Write down the approximate pH value and set the cup aside. (If

    using a garden soil pH tester kit, pour 1/4 teaspoon of the contents of the vinegar cup into the test

    container, and add 1/4 teaspoon of the test solution. Cover the test tube and shake once or twice

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    to mix, or stir if necessary. Compare with the color chart provided in the kit, and record the

    result.)

    - Dip an unused, clean strip of pH paper in the ammonia cup for about 2 seconds and immediately

    compare with the color chart. Write down the approximate pH value and set the cup aside. (If

    using a garden soil pH tester kit, repeat the same process in step 6 using the contents of the

    ammonia cup instead of the vinegar cup.)

    - Dip an unused, clean strip of pH paper into the water cup for about 2 seconds and immediately

    compare with the color chart. Write down the approximate pH value. (If using a garden soil pH

    tester kit, repeat the same process above using the contents of the water cup instead of the

    ammonia cup.)

    Questions and Answers

    Is vinegar an acid or a base?

    Vinegar is an acid, and in this experiment it will display a pH of about 4. Vinegar at pH 4 turns pH

    paper yellow and most other pH indicators red.

    Is ammonia an acid or a base?

    Ammonia is a base and in this experiment it will display a pH of about 12. Bases turn most pH

    indicators blue.

    Did distilled water have a neutral pH?

    PURE distilled water would have tested neutral, but pure distilled water is not easily obtained

    because carbon dioxide in the air around us mixes, or dissolves, in the water, making it somewhat

    acidic. The pH of distilled water is between 5.6 and 7. To neutralize distilled water, add about 1/8

    teaspoon baking soda, or a drop of ammonia, stir well, and check the pH of the water with a pH

    indicator. If the water is still acidic, repeat the process until pH 7 is reached. Should youaccidentally add too much baking soda or ammonia, either start over or add a drop or two of

    vinegar, stir, and recheck the pH.

    Experiment 2: Determining the pH of Common Substances

    In this experiment you will use a pH indicator to measure the pH of some fruits, common

    beverages, and borax. Borax is a cleaning agent that some people add to their laundry detergent.

    It is available at grocery stores. Many foods and household cleaners are either acids or bases.

    Acids usually taste sour, and bases bitter. Household cleaners are poisons so you should never

    taste them.

    Materials

    - pH paper and color chart (range pH 2 to 12) or garden soil pH testing kit

    - 3 fresh whole fruits (lemon, lime, orange, or melon)

    - 3 beverages (cola, carbonated non-cola, milk)

    - 1/8 teaspoon borax

    - measuring spoons (1/4 and 1/8 teaspoons)

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    - 4 small, clear cups or glasses

    - 1 clean stirring spoon

    - notebook and pencil

    - paring knife

    Instructions- Cut each fruit in half, drying off the knife after each cut.

    - Place an unused strip of pH paper half-on and half-off the inside of the cut fruit. Leave until wet

    (about 2 seconds). Immediately compare with the color chart. Write down the approximate pH

    value of the fruit. (If using a garden soil pH tester kit, squeeze 1/4 teaspoon of juice from the cut

    fruit into the test container, and add 1/4 teaspoon of the test solution. Cover the test container

    and shake once or twice to mix, or stir if necessary. Compare with the color chart provided in the

    kit, and record the result.)

    - Repeat the same process for the other 2 fruits.

    - Label the 3 cups: one cola, another non-cola, and the third milk.

    - Pour each liquid into an appropriately labeled cup.- Dip an unused strip of pH paper into the cola, compare with the color chart, and record the

    result. Repeat the same process for the remaining beverages. Be sure to use a clean, unused strip

    of pH paper for each one. (If using a garden soil pH tester kit, pour 1/4 teaspoon of cola into the

    test container, and add 1/4 teaspoon of the test solution. Tightly press your finger over the top of

    the test container and shake once or twice to mix, or stir if necessary. Compare with the color

    chart provided in the kit, and record the result.)

    - Add 1/8 teaspoon borax to 1/4 cup distilled water and stir for about 2 minutes.

    - Dip an unused strip of pH paper in the borax mixture, compare with the color chart, and record

    the result. (If using a garden soil pH tester kit, pour 1/4 teaspoon of the borax/water mixture into

    the test container, and add 1/4 teaspoon of the test solution. Tightly press your finger over the topof the test container and gently shake, or stir if necessary. Compare with the color chart provided

    in the kit and record the result.)

    Questions and Answers

    Are lemons, limes and oranges acids or bases?

    These fruits all contain acids and taste sour. Lemons and limes have pH values near 2. Oranges

    may be slightly less acidic than lemons and limes, but your pH indicator may not be accurate

    enough to show the difference.

    Are colas and non-colas acids or bases?They are both acidic, primarily becasue they both contain carbon dioxide to make them fizz, and

    carbon dioxide and water produce carbonic acid. The pH of these beverages varies with the

    amount of carbon dioxide and other ingredients in them, but it is usually below 4.

    Was the milk acidic or basic?

    Milk can be slightly basic or slightly acidic depending on its age and how it was processed at the

    dairy.

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    Was the borax/water mixture acidic or basic?

    Borax contains a strong base and will turn most pH indicators blue. The approximate pH of the

    borax/water mixture is 9. Its alkaline properties make it an excellent cleaning agent, which is why

    some people use it to wash clothes.

    Experiment 3: Making a Natural pH Indicator

    In this experiment you will make your own pH indicator from red cabbage. Red cabbage contains a

    chemical that turns from its natural deep purple color to red in acids and blue in bases. Litmus

    paper, another natural pH indicator, also turns red in acids and blue in bases. The red cabbage pH

    indicator can be obtained by boiling the cabbage.

    Materials

    - sliced red cabbage

    - stainless steel or enamel pan or microwave casserole dish

    - 1 quart water

    - stove, microwave, or hotplate

    - white vinegar

    - ammonia or baking soda

    - clear, non-cola beverage

    - 3 glass cups (preferably clear)

    - measuring spoons

    - 3 clean teaspoons for stirring

    - measuring cup (1/4 cup)

    - notebook and pencil

    Instructions- Boil cabbage in a covered pan for 30 minutes or microwave for 10 minutes. (Don't let water boil

    away.)

    - Let cool before removing the cabbage.

    - Pour about 1/4 cup of cabbage juice into each cup.

    - Add 1/2 teaspoon ammonia or baking soda to one cup and stir with a clean spoon.

    - Add 1/2 teaspoon vinegar to second cup, stir with a clean spoon.

    - Add about 1 teaspoon clear non-cola to the last cup and stir with a clean spoon.

    - After answering the first two questions for this experiment, pour the contents of the vinegar cup

    into the ammonia cup.

    Related Experiment: Neutralizing Acids or Bases Using a Garden Soil pH Tester Kit

    - Pour 1/4 teaspoon of the contents of the vinegar cup into the test container, and add 1/4

    teaspoon of the test solution. Seal the top of the test container with your finger, shake once or

    twice, or stir if necessary, and compare with the color chart. Then pour about 1/4 teaspoon of the

    contents of the ammonia cup into the test container. Mix it and compare with the color chart.

    What happens to the pH ? What would happen if you added more of the ammonia mixture? (For

    answers: see questions 3 and 4.)

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    Questions and Answers What color change took place when you added vinegar to the cabbage

    juice? Why?

    The vinegar and cabbage juice mixture should change from deep purple to red, indicating that

    vinegar is an acid.

    Did the ammonia turn the cabbage juice pH indicator red or blue? Why?The ammonia and cabbage juice mixture should change from deep purple to blue, because

    ammonia, like baking soda, is a base, which reacts chemically with the pH indicator, turning it blue.

    What happens to the color if you pour the contents of the vinegar cup into the ammonia cup?

    You should find that the acid and base are neutralized, changing the color from blue or red to

    purple, which is the original, neutral color of the cabbage juice

    If you were to gradually add vinegar to the cup containing the baking soda (or ammonia) and

    cabbage juice, what do you think would happen to the color of the indicator? Try it, stirring

    constantly.

    As you add more vinegar, the acid level increases and the color becomes red.

    Is the non-cola soft drink acidic or basic?

    It is acidic and turns the cabbage juice pH indicator red.

    Experiment 4: Measuring Soil pH

    In this experiment you will collect soil and measure its pH. Soil pH is one of several important

    conditions that affect the health of plants and animals. In addition, you will also be asked to survey

    the plants and animals that live in the area where you collected the soil. Area surveys provide

    information about how well plants and animals can live under different conditions.

    For this experiment, you will need an inexpensive garden soil pH test kit, which may be obtained

    from lawn and garden stores or nurseries.

    Materials

    - garden soil pH test kit

    - distilled water

    - 2 cups soil from each of two or three different locations (some of the soil will be needed for the

    "Soil Buffering" experiment)

    - measuring spoons

    - digging tool

    - self-sealing plastic bags

    - notebook and pencil

    Instructions

    - Pick two or three different soil locations, such as a garden, wooded area, city park, or meadow.

    Ask an adult to go with you.

    - At each location, observe the plants and animals living in or rooted on these soils, especially

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    those that are in greatest numbers. Write down as much as you can about what you find. Dig

    down about 2 inches, scoop out 2 cups of soil, and seal it in a plastic bag for later use. Label each

    plastic bag. Be sure to clean your digging tool after collecting soil samples at each location.

    - Measure the pH of each soil sample following the directions provided in the garden soil pH test

    kit, and record the approximate pH of each soil sample.

    - Save the excess soil from each site for use in the "Soil Buffering" experiment.

    Questions and Answers

    Were there any big differences between the plant and animal life at each location?

    Some types of plants and animals are able to live in acid soils, while others are not. Be aware,

    however, that many factors, not just the soil acidity, determine the types of plants and animals

    that occur at a particular site.

    Were any of your soil samples acidic?

    Some plants require acid soils to grow and thrive. For example, pine trees, azaleas,

    rhododendrons, cranberries, blueberries, potatoes, and tomatoes prefer acid soils. However, most

    plants thrive only in soils of pH 6 to 7.

    Were any of your soil samples basic?

    Some soils, such as in many midwestern United States, contain a lot of limestone and are alkaline.

    In those locations, people often add sulfate, such as ammonium bisulfate to soil to make it less

    basic.

    Experiment 5: Soil Buffering

    Soil sometimes contains substances, like limestone, that buffer acids or bases. Some salts in soil

    may also act as buffers. In this experiment you will find out if soil from your lawn, garden, or

    school can buffer acids. You will observe the pH change of an acid mixture poured over soil in a

    filter. If the water collected from the filter is less acidic than the original mixture, then the soil is

    buffering some of the acid. If it does not change, then the soil may not be capable of buffering

    acids. Since the buffering capability of soils differs, you may want to do this experiment with

    several different soil types including those collected for the "Soil pH" experiment.

    Materials

    - pH paper and color chart (pH range 2 to 10) or garden soil pH test kit

    - about 2 cups of soil from a garden, wooded area, lawn, or school yard

    - distilled water- white vinegar

    - measuring cups and spoons

    - stirring spoon

    - large funnel

    - 3 coffee filters

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    - paper cup

    - notebook and pencil

    Instructions

    - Pour 1 teaspoon of vinegar into 2 cups of distilled water, stir well, and check the pH with either

    pH paper or a garden soil pH testing kit. The pH of the vinegar/water mixture should be about 4. Ifit is below that, add a sprinkle of baking soda, stir well, and recheck the pH; but if it is above pH 4,

    add a drop or two of vinegar and again recheck the pH.

    - Put 1 coffee filter into the funnel, and fill the filter with soil from one location. Do not pack the

    soil down.

    - Hold the filter over a paper cup and slowly pour the vinegar/water mixture over the soil until

    some water collects in the paper cup (the filter may clog quickly, but you need only a small

    amount of water).

    - Check the pH of the collected water using either pH paper or a garden soil pH testing kit and

    record the results.

    - Repeat the experiment with other soil samples, using a new coffee filter for each sample.

    Questions and Answers

    Did the pH of the collected water stay the same as the original mixture, increase, or decrease?

    If the pH stayed the same, the soil did not buffer the acid. Each pH value above 4 indicates that the

    soil buffered increasing amounts of the acid. Even soil capable of buffering acids can be

    overpowered if enough acid is added. As more acid is added to the soil, the buffering capability

    decreases, and the water from the filter becomes more acidic.

    What can you add to the soil to increase its buffering capability?

    Limestone can be added, but it takes weeks to months for the limestone to work into the soil.

    Experiment 6: Observing the Influence of Acid Rain on Plant Growth

    Acid rain most often damages plants by washing away nutrients and by poisoning the plants with

    toxic metals. It can, however, have direct effects on plants as well. In this experiment you will

    observe one of the direct effects of acid water on plant growth. The experiment will take about 2

    weeks.

    Materials

    - 4 cups or jars

    - distilled water- white vinegar

    - measuring cups

    - stirring spoon

    - 2 cuttings of a philodendron plant (1 leaf and small amount of stem)

    - 2 cuttings of a begonia or coleus plant (1 leaf and small amount of stem)

    - notebook and pencil

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    Instructions

    - Pour 1 teaspoon of vinegar into 2 cups of distilled water, stir well, and check the pH with either

    pH paper or a garden soil pH testing kit. The pH of the vinegar/water mixture should be about 4. If

    it is below pH 4, add a sprinkle of baking soda, or a drop of ammonia, stir well, and recheck the pH.

    If it is above pH 4, add a drop or two of vinegar and again recheck the pH.

    - Measure the pH of the distilled water using either pH paper or a garden soil pH testing kit. If the

    pH is below 7, add about 1/8 teaspoon baking soda, or a drop of ammonia, stir well, and check the

    pH of the water with the pH indicator. If the water is still acidic, repeat the process until pH 7 is

    reached. Should you accidentally add too much baking soda or ammonia, either start over again or

    add a drop or two of vinegar, stir, and recheck the pH.

    - Put one of the following labels on each cup or jar:

    - water philodendron

    - acid philodendron

    - water begonia (or coleus)

    - acid begonia (or coleus)

    - Pour about a cup of distilled water into the water-philodendron and water-begonia cups.

    - Pour about a cup of the vinegar/water mixture into the acid-philodendron and acid-begonia

    cups.

    - Put one philodendron cutting into each philodendron labeled cup, covering the stem and part of

    the leaf with the liquid.

    - Put one begonia cutting into each begonia-labeled cup, covering the stem and part of the leaf

    with the liquid.

    - Set the cups where they are not likely to be spilled and where they will receive some daylight.

    - About every 2 days, check to be sure that the plant cuttings are still in the water or

    vinegar/water. You may need to add more liquid if the cups become dry.

    - After 1 week, compare the new root growth of each plant in distilled water with the new root

    growth of its corresponding plant in acid water. Record the results.

    - After 2 weeks, again observe the plant cuttings for new root growth, and record the results.

    Questions and Answers

    Which plant cuttings had the fastest root growth, those in distilled water or those in acid water?

    The plants grown in distilled water should grow faster than plants grown in acid water. Acid water,

    like acid rain, can directly damage plants and slow or stop new growth.

    Experiment 7: Observing Buffers in Lakes, Ponds, and Streams

    In this experiment you will observe the effects of limestone on the acidity of water. Some areas of

    the nation have a lot of limestone in lake bottoms and in soil, which helps neutralize the effects of

    acid rain. Crushed limestone is sometimes added to lakes, ponds, and other aquatic areas to help

    neutralize the effects of acid rain, thus preserving important aquatic systems until the source of

    acid rain can be reduced. Crushed limestone is easily obtained from local lawn and garden stores

    or nurseries.

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    Materials

    - pH paper and color chart (pH range 2 to 7) or garden soil pH testing kit

    - white vinegar

    - distilled water

    - measuring cup and spoon

    - 2 stirring spoons

    - 1/2 cup crushed hydrated limestone or spray limestone

    - 2 cereal bowls (about 2 cup size)

    - plastic wrap

    - notebook and pencil

    Instructions

    - Label one bowl vinegar; the other one vinegar plus limestone.

    - Pour 1/4 cup crushed limestone into one bowl.

    - Pour 1 teaspoon of vinegar into 2 cups of distilled water, stir well, and check the pH with either

    pH paper or a garden soil pH testing kit. The pH of the vinegar/water mixture should be about 4. Ifit is below pH 4, add a sprinkle of baking soda, stir well, and recheck the pH; but if it is above pH 4,

    add a drop or two of vinegar and again recheck the pH.

    - Pour about 1 cup of the vinegar/water mixture over the limestone in the cereal bowl and stir

    with a clean, dry spoon.

    - Pour the remaining vinegar/water mixture into the other cereal bowl.

    - Check the pH of the vinegar/water mixture over the limestone and record it.

    - Cover each bowl with plastic wrap to prevent evaporation.

    - Every day for 6 days, stir the contents of each bowl with a clean, dry spoon and about 4 or more

    hours later (after the limestone has settled), test the pH of the water mixture in each bowl and

    record the result.

    Questions and Answers

    Did the pH of the vinegar/water mixture over the limestone become more or less acidic during the

    6-day period? Why?

    The water mixture should have become less acidic, changing from about pH 4 to as much as pH 6,

    depending on the water content of the limestone you used.

    Does crushed limestone buffer the acid?

    Yes, by neutralizing it.

    Did the pH of the vinegar/water mixture in the other bowl (without limestone) change during the

    6-day period?

    The pH of the bowl without limestone should not have changed.

    Experiment 8: Looking at Acid's Effects on Metals

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    When acids and metals come in contact with each other, the metal is gradually dissolved away in a

    chemical reaction. In this experiment you will observe this reaction for yourself, but you will need

    patience. The chemical effect of acids on metals may take at least five days for the human eye to

    see, even though the reaction starts as soon as the acid contacts the metal.

    Materials- pH paper and color chart (pH range 2 to 7) or garden soil pH testing kit

    - 2 small, clear glasses (nonmetal)

    - 2 clean copper pennies (use pennies minted before 1983)

    - white vinegar or fresh-squeezed lemon juice

    - distilled water

    - plastic wrap

    - notebook and pencil

    Instructions

    - Label one glass water and the other vinegar or lemon juice depending on which acid you use.

    - Place one penny in each glass. Be sure to use pennies minted before 1983 because pennies

    minted after that time have a different chemical composition.

    - Barely cover one of the pennies with either vinegar or lemon juice.

    - Dip a strip of pH paper into the vinegar, or lemon juice, for about 2 seconds, compare with the

    color chart, and record the result. Or use a garden soil pH test kit.

    - Add enough distilled water to the glass labeled water to barely cover the other penny.

    - Dip a strip of pH paper into the distilled water for about 2 seconds and compare with the color

    chart. Or use a garden soil pH test kit. If the pH is below 6, add a tiny amount (less than 1/8

    teaspoon) of baking soda, or a drop of ammonia, and recheck the pH. Repeat this process until the

    pH is between 6 and

    - Record the pH of the water.

    - Seal the top of each glass with plastic wrap to prevent evaporation.

    - Place in a safe, dry place for about 5 days.

    - After about 5 days, observe the changes that occurred in each glass.

    - At the end of the experiment, wash off the pennies with water, and pour the contents of the

    glasses down the sink (do not drink).

    Questions and Answers

    What change, if any, took place in the water glass after 5 days?

    There should be no change

    What change, if any, took place in the vinegar (or lemon juice) glass after 5 days?

    The liquid should be bluish-green. The bluish-green substance in the vinegar, or lemon juice,

    comes from the copper in the penny. It is a byproduct of the chemical reaction in which the acid in

    the vinegar, or lemon juice, very gradually eats away the penny.

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    When you rinsed off the pennies, were you surprised that they both looked about the same as

    they did at the beginning of the experiment (assuming you used clean pennies)?

    The chemical reaction between the acid and the copper penny is so slow that you cannot see any

    difference in the shape of the metal in just 5 days, at least not with your eye alone. You may see

    some changes after about two weeks, especially at the edge of the penny.

    Science projects about plants and botany

    Learn how to prepare

    award-winningscience

    fair projects.

    1. How do different

    conditions affect the

    speed at which fruit and

    vegetables ripen?

    Temperature, light,

    placement in sealed

    bags, exposure to other

    ripe fruit--all havedifferent effects on

    different fruits and

    vegetables. Design an

    experiment to test two

    or more of these

    variables.

    Background Info:

    Ethylene gas is the

    ripening agent that

    many fruits and

    vegetables produce

    naturally. Ethylene

    causes them to ripen--

    and then overripen.

    While refrigeration and

    humidity slow the

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    effects of ripening, they

    don't stop the

    production of ethylene

    gas. The more the fruit

    ripens, the more

    ethylene gas it makes.

    This has a big effect on

    how--and when--farmers

    harvest their fruits and

    vegetables for market.

    Most commercial

    tomatoes are picked

    before ripening is

    completed, so the fruit

    won't spoil before it gets

    to your market. But

    picking early also means

    the tomato spends less

    time on the vine, where

    ethylene would help

    build more of the sugars

    and acids that create tip-

    top tomato flavor.

    2. How do different

    types of fertilizers affect

    plant growth?

    Fertilizers differ in their

    amounts of the nutrients

    nitrogen, phosphorus

    and potassium. Get

    differentfertilizersfrom

    a garden shop or nursery

    and apply them to

    groups of the same

    plant. Do the different

    fertilizers change how

    the plants grow? You

    could measure height,

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    width, number of leaves,

    how fast the plants

    grow, number of flowers

    or yield.

    3. What happens when

    you grow sweet

    potatoes next to other

    plants?

    Compare how fast the

    other plants grow at

    different distances from

    sweet potatoes.

    Remember to grow

    some control plants

    nowhere near the sweet

    potato.

    Background Info:

    Allelopathy is a chemical

    process that a plant uses

    to keep other plants

    from growing too close

    to it. Some plants thatuse allelopathy are black

    walnut trees,

    sunflowers,

    wormwoods,

    sagebrushes, and trees

    of heaven.

    There are several ways

    in which an allelopathic

    plant can release itsprotective chemicals:

    Volatilization -

    Allelopathic

    trees release a

    chemical in the

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    form of a gas

    through small

    openings in their

    leaves. Other

    plants absorb

    the toxic

    chemical and

    die.

    Leaching - Some

    plants store

    protective

    chemicals in the

    leaves they

    drop. When the

    leaves fall to theground, they

    decompose,

    giving off

    chemicals that

    protect the

    plant.

    Exudation -

    Some plants

    releasedefensive

    chemicals into

    the soil through

    their roots.

    Those chemicals

    are absorbed by

    the roots of

    other nearby

    plants, which are

    damaged.

    4. How do different

    treatments change how

    fast seeds sprout?

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    You can find out how

    quickly seeds sprout

    under different

    temperatures, or after

    being soaked for

    different times or in

    different liquids. Or, see

    how one kind of

    treatment affects

    different types of seeds.

    5. How close does a

    pesticide have to be to

    protect a plant?

    Grow a number of

    groups of the same

    plant. Apply a Bt-based

    insecticide directly to

    the plant according to

    the directions on the

    package and at various

    distances from the

    plants. Compare the

    amount of insect

    damage to each group of

    plants. You might also

    look at how big or fast

    each group of plants

    grows.

    6. How does soil pH

    affect the pH of waterthat touches the soil?

    Gather different types of

    soil. Put some of each

    type in a cup and check

    out the pH. Then add

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    water to the cups, and

    mix. Wait for the soil to

    settle and measure the

    pH of the water. Be sure

    you use water from the

    same source for each

    soil. Find out more about

    soil.

    Background Info: A pH

    meter can be found at

    almost any garden shop

    or nursery.

    The pH scale - Just about

    every substance is acidic,

    basic or neutral. The acidor base nature of a

    substance is measured

    by a pH scale that runs

    from 0 to 14. Substances

    from 0 to 7 are

    considered acid;

    substances from 7 to 14

    are basic. Seven, the pH

    of pure water, is

    considered "neutral."The pH of your blood is

    about 7.35. Most plants

    grow best around pH

    7.0. Some--like

    blueberries, azaleas and

    rhododendrons--like acid

    soil with a pH from

    about 5 to 6.

    7. Which way is up?

    Many seeds and bulbs

    have a definite top and

    bottom. What happens if

    you plant them upside

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    down or sideways? Will

    the seeds still grow; will

    it take longer for leaves

    to start showing up?

    What happens if youchange a seed's

    direction once it starts to

    sprout? Many seeds like

    beans can be sprouted in

    moist cotton or paper

    towels. What happens if

    you turn the seed 90 or

    180 degrees from right

    side up every few days

    after it sprouts?

    You can take it a step

    further by using a record

    player turntable to

    simulate changing

    gravity's pull on seeds.

    You'll want to know

    more about the chemical

    auxin, which affects

    where roots and stemsgrow.

    Sprout bean seeds for 3

    days in moist paper

    towels inside pieces of

    folded aluminum foil.

    Then tape one or more

    packets on the turntable

    and set it for 78 RPM.

    Allow the machine to

    rotate continuously for 5days. After the 5 days

    are up, turn off the

    record player and

    without changing the

    position of the foil, open

    them up and observe

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    the beans.

    The rotating turntable

    creates a gravity with an

    outward force instead of

    the normal down.

    8. Roots Restrictions

    Does the amount of

    room a plant has for

    roots make a difference

    in how big a plant will

    grow, regardless of how

    much fertilizer the plant

    is given? Plant seeds in a

    variety of different-sized

    containers using

    vermiculite or other soil-

    less material, so you will

    be able to give each

    plant a measured

    amount of fertilizer. Or

    plant a number of plants

    in the same size

    containers and vary the

    amount of fertilizer and

    see what happens. Be

    sure to use small enough

    containers so that root

    growth really will be

    constricted.

    9. Can different colorsand types of cloth

    attract or repel insects

    from plants?

    Plant a number of

    groups of the same type

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    of plant near each other,

    but far enough apart to

    surround each set with

    several feet of fabric. Or

    select several of the

    same kind of bush in one

    yard. You want to use

    the same type of plant in

    the same place, so all of

    the plants will have the

    same potential for insect

    damage.

    Surround each group of

    plants with a different

    color fabric. Be surewater can penetrate the

    fabrics. At set intervals,

    record all the insects you

    can find on each plants

    and any signs of insect

    damage on the plant. It

    is a good idea to check

    reference sources for

    common insect

    problems of the type ofplants you are using.

    10. The effects of light

    on seedlings

    germination

    How do light and dark

    conditions affect the

    germination and growth

    of seedlings?

    Materials: 20 bean

    seeds, 2 Ziploc bags, 2

    damp paper towels, desk

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    lamp.

    Procedure: Separate

    bean seeds into two

    different piles with equal

    number of seeds (10seeds in each pile). Wet

    the paper towels until

    completely dampened.

    Place the dampened

    paper towels in the

    Ziploc bags, and then

    place 10 seeds on top of

    the paper towels in each

    bag. Make sure the

    seeds are on the papertowel in the bag and

    close the bag, but not

    completely (about 3/4

    way closed). Wrap one

    of the Ziploc bags

    completely in aluminum

    foil. Leave the other one

    uncovered. Place both

    Ziploc bags under a desk

    lamp. After 7 days, checkthe bag that has been in

    the light as well as the

    bag that has been

    wrapped in aluminum

    foil. Compare the

    germinated seeds. You

    should definitely see a

    difference between the

    two. You should note

    mainly the color and

    stem length differences

    between the seedlings

    that germinated in light

    and those that

    germinated in darkness.

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    What differences did

    you observe between

    seedlings that

    germinated in the light

    and in the dark? (color

    of leaves, length of

    stems, etc.) What caused

    those differences?

    11. What affect does the

    brightness of light have

    on the growth rate of a

    plant?

    How do light and dark

    conditions affect the

    germination and growth

    of seedlings?

    Materials: Greenhouse

    or sunny window sill, 10

    bean seeds, 10 small

    pots, water, ruler,

    potting soil, pencil.

    Procedure: Fill the 10

    small pots with equal

    amounts of dampened

    potting soil. With a

    pencil, make holes about

    2 centimeters deep in

    each pot. Place the 10

    bean seeds, one per pot,

    and cover the seeds with

    some of the soil. Place 5of the pots in the

    greenhouse or on a

    window sill on the sunny

    side of the house. Place

    the other 5 on a window

    sill that does not receive

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    bright sunlight. Seeds

    will germinate within 7

    days, and you can begin

    making stem

    measurements. Take

    stem measurements for

    14 days. Be sure to

    water the plants as

    needed. Note the

    difference in stem length

    for each set of plants,

    and write down your

    observations.

    What differences did

    you observe betweenseedlings that grew in

    the bright sunlight

    compared to less bright

    light? (color of leaves,

    length of stems, etc.)

    What caused those

    differences?

    12. Does crowding

    affect plant growth?

    Determine the effects of

    growing plants close

    together vs. growing

    plants farther apart.

    Materials: 6 medium-

    sized pots, 10 bean

    seeds, potting soil,water, ruler, large

    measuring cup, desk

    lamp, pencil.

    Procedure: Fill all pots

    with an equal amount of

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    potting soil. Be sure that

    the soil has been

    dampened with water.

    Using a pencil, make 5

    holes about 2

    centimeters (cm) deep in

    the soil of one pot. Place

    seeds in each hole

    making sure that they

    are spaced relatively

    close but equal distance

    from each other within

    the pot. Place the

    remaining 5 seeds, 1 in

    each of the remaining 5

    pots, about 2 cm deep.

    Cover the seeds with

    soil. Place all the pots

    underneath a large desk

    lamp so that each pot

    receives full light. Be

    sure to water each plant

    as needed. The seeds

    will germinate in about 7

    days, and you will be

    able to begin making

    stem measurements.

    Take measurements for

    14 days. Note the

    difference in stem length

    for each plant and write

    down your observations.

    What differences did

    you observe between

    seedlings that were

    crowded and those that

    were not? (color of

    leaves, length of stems,

    etc.) What caused those

    differences?

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    13. Does Colored Mulch

    Affect Soil

    Temperature?

    To determine if different

    colored plastic (or

    mulch) on the

    soilsurfaceaffects the

    temperature of soil.

    Materials: Digital

    thermometer; potting

    soil; 8 pots; colored

    mulch in black, white,

    and red; window sill with

    full sun; tape. For the

    colored mulch, you can

    use different paint colors

    to paint black plastic, or

    you might use different

    colored plastic bags or

    mulch from the store.

    Procedure: Fill pots with

    equal amounts of soil.Place 2 uncovered pots

    on the window sill where

    there is full sun. Cover 2

    pots with black plastic, 2

    pots with white plastic,

    and 2 pots with red

    plastic. Make sure the

    plastic covers the top of

    the pot, and tape it to

    the pot. Place these pots

    on the window sill. In 24

    hours, record your first

    temperature

    measurements. You can

    do this by sticking the

    temperature probe in

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    the drainage hole of the

    pot. For 10 days, record

    the temperatures for

    each pot in the morning

    and afternoon. Note the

    differences in

    temperature among the

    colors, and record

    observations.

    Which pot had the

    highest soil

    temperature? What

    color kept the soil the

    coolest? Why do you

    think that some mulchcolors make the soil

    hotter or cooler? How

    does this affect plant

    growth (see project 14)?

    14. Does Colored Mulch

    Affect the Growth Rate

    of a Plant?

    To determine if colored

    plastic (mulch) will affect

    the stem length of

    plants.

    Materials: 8 pots; 8 bean

    seeds; colored plastic in

    red, black, and white;

    ruler; water; toothpicks;

    greenhouse or windowthat receives full sun;

    tape; potting soil; pencil;

    small cup.

    Procedure: Fill pots with

    equal amounts of

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    potting soil. Make a hole

    about 2 cm long in each

    pot using a pencil, and

    place 1 seed in each pot.

    Cover seed with loose

    soil. Mark where you

    planted the seed with a

    toothpick. Cut plastic in

    pieces large enough to

    cov


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