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Microbiology Case StudiesProfessor Patricia Sidelsky
Microbiology 11330
Rowan University
Summer Session II
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Case Study Problems
1 Read t!e case study Underline t!e significant facts
" Researc! t!e case by using te#t reference $ Prescott% &arley% and 'lein(and Internetresources t!at are included wit! case studies )dditional medical resources s!ould also be
documented
3 *rite out t!e answers in com+lete sentences ,!e answers s!ould eit!er be !andwritten on notebook +a+er$ lined( or com+leted on a word +rocessor
- ,!e answers s!ould reflect scientific and medical accuracy )nswers s!ould be
t!oug!tful and reflect com+re!ension of t!e ty+e of bacterial infection
. /ames of bacteria s!ould be correctly written#am+le in italics wit! 2enus ca+italied and s+ecies in lower case
Sta+!ylococcus aureus
4r
Sta+!ylococcus aureus5 2rades will be based u+on t!e criteria stated
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Case Study 4ne
Con6unctivitis in t!e Classroom
Mrs )llen% a teac!er at C!erokee &ig! Sc!ool% noticed t!at one of t!e girls in t!e frontrow of !er first +eriod 7iology class seemed to !ave red and teary eyes Initially w!en !ea++roac!ed t!e student% 8ennie% s!e believed !er to be crying and u+set *!en !e9uestioned 8ennie% s!e found t!at !er eyes were red and a++eared to be very irritated
S!e referred !er to t!e nurse U+on closer e#amination% t!e nurse% Mrs :ivingston% foundt!e student;s rig!t eye to be blood s!ot ,!e lower lid was swollen and brig!t red ) t!ick
disc!arge was found in t!e corner of t!e eye 8ennie com+lained t!at !er eye t!robbed
Mrs :ivingston told 8ennie not to touc! !er eye S!e called t!e +arents and suggested t!at
8ennie see a +!ysician immediately for t!e correct diagnosis and treatment 8ennieremained in t!e nurse;s office until !er +arents came to take !er to t!e doctor
Case Study
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Case Study ,wo
,!e Case of t!e 2raed 2ardener
) 5" year old botanist and !orticulturist was admiring !er garden of lilies w!en s!e s+otteda weed in t!e midst of !er flowers S!e !ad left !er gloves in t!e !ouse Unfortunately
w!en s!e went to remove t!e offensive weed% t!e tiny t!orny base gas!ed !er !and S!e
immediately went into t!e !ouse and was!ed t!e wound and added some to+ical antibiotic
6ust in case &owever% wit!in a day s!e noticed a +uffy red swelling around t!e gas! 7y t!esecond day s!e became concerned w!en s!e awoke to find t!at w!en s!e tried to get u+ out
of bed s!e found walking difficult S!e immediately called !er son w!o came to take !er tot!e R of t!e local Eirtua &os+ital immediately 7y t!e time s!e reac!ed t!e !os+ital s!e
began to e#+erience difficulty wit! s+eec! and muscle s+asms ,!e doctor ins+ected t!e
wound site and noted t!e now severe swelling &e was more concerned by !is +atient;s
growing res+iratory distress and acute muscle s+asms
,!e +!ysician contacted t!e woman;s +rimary care give &e found t!at s!e !ad not received
a ,etanus booster in more t!an ten years ,roubled by t!is information% !e immediately
intubated !is +atient and administered counter measures
Case Study
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5 *!at ot!er organisms belong to t!is 2enus t!at are medically significant= Aescribe at
least t!ree
LINKS
Med info > !tt+?@@medinfoufledu@year"@[email protected]@bugs@clostet!tmleMedicine > !tt+?@@wwwemedicinecom@med@to+ic"".-!tm
Aoc'aiser;s Microbiology Page>
!tt+?@@studentccbcmdedu@courses@bio1-1@lecguide@unit1@+rostruct@diseases@ctetani@ctet
ani!tmlCAC ,etanus immuniation >
!tt+?@@wwwcdcgov@ncidod@diseases@submenus@subFtetanus!tm
CAC> 2eneral Information >
!tt+?@@wwwcdcgov@ncidod@diseases@submenus@subFtetanus!tmCAC> 7otulism > !tt+?@@wwwcdcgov@ncidod@dbmd@diseaseinfo@botulismFg!tm
Mayo Clinic > !tt+?@@wwwmayocliniccom@!ealt!@gangrene@& !tt+?@@!ealt!linkmcwedu@[email protected]""D"!tml
http://medinfo.ufl.edu/year2/mmid/bms5300/bugs/clostet.htmlhttp://www.emedicine.com/med/topic2254.htmhttp://student.ccbcmd.edu/courses/bio141/lecguide/unit1/prostruct/diseases/ctetani/ctetani.htmlhttp://student.ccbcmd.edu/courses/bio141/lecguide/unit1/prostruct/diseases/ctetani/ctetani.htmlhttp://www.cdc.gov/ncidod/diseases/submenus/sub_tetanus.htmhttp://www.cdc.gov/ncidod/diseases/submenus/sub_tetanus.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/botulism_g.htmhttp://www.mayoclinic.com/health/gangrene/HQ00737http://healthlink.mcw.edu/article/954992292.htmlhttp://medinfo.ufl.edu/year2/mmid/bms5300/bugs/clostet.htmlhttp://www.emedicine.com/med/topic2254.htmhttp://student.ccbcmd.edu/courses/bio141/lecguide/unit1/prostruct/diseases/ctetani/ctetani.htmlhttp://student.ccbcmd.edu/courses/bio141/lecguide/unit1/prostruct/diseases/ctetani/ctetani.htmlhttp://www.cdc.gov/ncidod/diseases/submenus/sub_tetanus.htmhttp://www.cdc.gov/ncidod/diseases/submenus/sub_tetanus.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/botulism_g.htmhttp://www.mayoclinic.com/health/gangrene/HQ00737http://healthlink.mcw.edu/article/954992292.html8/13/2019 New Case Study Booklet-1
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Case Study ,!ree
Ra+id Stre+
)t C!ildrens &os+ital$ C&4P( t!e R and Clinics are always busy ) .>year old w!ite male
c!ild in good general !ealt! and +!ysical condition was +resented at t!e Saturday walk>inclinic by !is mot!er &e was broug!t in because !e !ad a fever% was cranky and !ad
com+lained of a sore t!roat for about "- !ours 4n +!ysical e#amination by t!e attending
resident% t!e +atient !ad a fever of 3D oC &e !ad considerable swelling and drainage of t!e
+!aryn# and in t!e con6unctivae &is tonsils were enlarged and coated wit! a w!ite +atc!ye#udate &e !ad a red t!roat and swollen anterior cervical lym+! nodes &is ears were clear
&is c!est sounded clear and !e !ad no additional remarkable findings on routine
e#amination ) ra+id Stre+ test run )S)P by t!e lab was +ositive 7acteria grown from a
t!roat swab taken at t!e time of e#amination indicated gram +ositive 7eta !emolytic
bacteria
1 *!at would be your +resum+tive diagnosis for t!is c!ild= *!y=" *!at diagnostic testing would be indicated to follow t!is e#am=
3 *!at is t!e most likely treatment for t!is illness= *!y is it im+ortant=
- *!at factors of t!is case allowed you to make a +resum+tive diagnosis=
. *!at serious conse9uences can result if treatment for t!is infectious agent !ad notoccurred in a timely manner=
:I/'S
Sore t!roat reference > !tt+?@@wwwc!es!ire>medcom@services@bugs@stre+t!tml
Stre+tococcus > grou+ ) >
!tt+?@@wwwcdcgov@ncidod@dbmd@diseaseinfo@grou+astre+tococcalFg!tm
Stre+tococcus > grou+ 7 !tt+?@@wwwcdcgov@ncidod@dbmd@diseaseinfo@grou+bstre+Fg!tm
http://www.cheshire-med.com/services/bugs/strept.htmlhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/groupastreptococcal_g.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/groupbstrep_g.htmhttp://www.cheshire-med.com/services/bugs/strept.htmlhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/groupastreptococcal_g.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/groupbstrep_g.htm8/13/2019 New Case Study Booklet-1
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More :inks
!tt+?@@wwwkids!ealt!org@+arent@infections@lung@stre+Ft!roat!tml!tt+?@@wwwmayocliniccom@!ealt!@stre+>t!roat@AS00"50
!tt+?@@wwwemedicine!ealt!com@stre+Ft!roat@articleFem!tm
!tt+?@@te#tbookofbacteriologynet@stre+tococcus!tml
!tt+?@@wwwabbottdiagnosticscom@HourF&ealt!@Res+iratoryFInfections@Stre+cfm!tt+?@@wwwcdcgov@ncidod@dbmd@diseaseinfo@grou+astre+tococcalFg!tm
!tt+?@@wwwwebmdcom@a>to>>guides@Ra+id>stre+>test>for>stre+>t!roat
http://www.kidshealth.org/parent/infections/lung/strep_throat.htmlhttp://www.mayoclinic.com/health/strep-throat/DS00260http://www.emedicinehealth.com/strep_throat/article_em.htmhttp://textbookofbacteriology.net/streptococcus.htmlhttp://www.abbottdiagnostics.com/Your_Health/Respiratory_Infections/Strep.cfmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/groupastreptococcal_g.htmhttp://www.webmd.com/a-to-z-guides/Rapid-strep-test-for-strep-throathttp://www.kidshealth.org/parent/infections/lung/strep_throat.htmlhttp://www.mayoclinic.com/health/strep-throat/DS00260http://www.emedicinehealth.com/strep_throat/article_em.htmhttp://textbookofbacteriology.net/streptococcus.htmlhttp://www.abbottdiagnostics.com/Your_Health/Respiratory_Infections/Strep.cfmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/groupastreptococcal_g.htmhttp://www.webmd.com/a-to-z-guides/Rapid-strep-test-for-strep-throat8/13/2019 New Case Study Booklet-1
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Case Study our
Sta+!ylococcus and a Sim+le Scratc!
)n eig!teen year old student in an automotive tec!nology class at t!e local !ig! sc!ool
com+lained of a +ain in !is !and &is mot!er was concerned because !e was feveris! and
let!argic% &e wanted to stay !ome from sc!ool ,!e young man reluctantly admitted to !is
mot!er t!at !e !ad cut !is !and during !is automotive class w!ile working on a car engine&e !ad was!ed !is !and wit! soa+ and water immediately at t!e faucet in t!e classroom &e
!ad also covered t!e gas! wit! a band aid )lt!oug! !is teac!er !ad given !im a +ass to t!esc!ool nurse to a follow u+% t!e student conveniently forgot w!en t!e bell rang and !e !ad
to get to !is ne#t class on time
*!en 9uestioned by !is mot!er% t!e young man admitted to !aving diarr!ea and vomiting
t!e +revious day &is mot!er immediately made an a++ointment wit! t!e doctor ,!edoctor noticed t!at t!e boys arm was sensitive to touc! *!en e#amined t!e boys !ad
!ad an o+en sore wit! +us ,!e area around t!e in6ury was edematous ,!e +!ysicianimmediately noticed t!at !e also !ad +ur+lis!% red streaks running u+ !is arm from t!e site
of t!e in6ury ,!e doctor confirmed !is sus+icions w!en !e also observed enlarged lym+!
nodes under t!e arm wit! t!e o+en wound ,!e skin on t!e arm felt very warm and dry
Case Study
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3 *!at sym+toms are indicative of an infection caused by t!is organism=
- *!at are treatments of t!is serious ty+e of infection= *!at tests s!ould be done int!e clinical laboratory at t!e !os+ital to determine t!e antibiotic to be used= *!at
treatment s!ould begin immediately
. If untreated w!at additional measures would need to be administered to +revent a
widely disseminated infection=
:I/'s
!tt+?@@te#tbookofbacteriologynet@sta+!!tml> ,odar 4n :ine Microbiology
!tt+?@@wwwcfsanfdagov@Jmow@c!a+3!tml> 7ad 7ug 7ook
!tt+?@@wwwkids!ealt!org@+arent@infections@bacterialFviral@sta+!ylococcus!tml> 'ids&ealt!
!tt+?@@wwwemedicinecom@PA@to+ic"G0-!tm> eMedicine
!tt+?@@wwwcdcgov@/cidod@diseases@submenus@subFsta+!ylococcus!tm> CAC and
Sta+!ylococcus
!tt+?@@medicmedut!tmcedu@[email protected]!tm> Sta+!ylococcus
!tt+?@@wwwcdcgov@ncidod@d!9+@arFmrsa!tml> MRS)$ CAC(
!tt+?@@wwwmayocliniccom@!ealt!@mrsa@AS00G3.> MRS)$ Mayo Clinic(
!tt+?@@wwwnlmni!gov@medline+lus@ency@article@00G"51!tm> Med:ine
http://textbookofbacteriology.net/staph.htmlhttp://www.cfsan.fda.gov/~mow/chap3.htmlhttp://www.kidshealth.org/parent/infections/bacterial_viral/staphylococcus.htmlhttp://www.emedicine.com/PED/topic2704.htmhttp://www.cdc.gov/Ncidod/diseases/submenus/sub_staphylococcus.htmhttp://medic.med.uth.tmc.edu/path/00001456.htmhttp://www.cdc.gov/ncidod/dhqp/ar_mrsa.htmlhttp://www.mayoclinic.com/health/mrsa/DS00735http://www.nlm.nih.gov/medlineplus/ency/article/007261.htmhttp://www.nlm.nih.gov/medlineplus/ency/article/007261.htmhttp://textbookofbacteriology.net/staph.htmlhttp://www.cfsan.fda.gov/~mow/chap3.htmlhttp://www.kidshealth.org/parent/infections/bacterial_viral/staphylococcus.htmlhttp://www.emedicine.com/PED/topic2704.htmhttp://www.cdc.gov/Ncidod/diseases/submenus/sub_staphylococcus.htmhttp://medic.med.uth.tmc.edu/path/00001456.htmhttp://www.cdc.gov/ncidod/dhqp/ar_mrsa.htmlhttp://www.mayoclinic.com/health/mrsa/DS00735http://www.nlm.nih.gov/medlineplus/ency/article/007261.htm8/13/2019 New Case Study Booklet-1
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Peril in t!e Pinelands
Case ive
) young undergraduate student at Rowan University s+ecialiing in cology% c!ecked in at
!er college medical care center% re+orting a wide s+ectrum of !ealt! concerns S!e !adrecently com+leted a deer +o+ulation study as a +art of a researc! +ro6ect in t!e Pinelands
of Sout! 8ersey ) week after !er field work was com+leted% t!e student began to feel
fatigued and listless S!e e#+erienced an inability to concentrate and a general feeling ofmalaise S!e re+orted ac!es in !er legs w!ic! s!e attributed to walking miles t!roug! t!e
+ineland forests ,!e +!ysician noted a circular K ras!K about . inc!es in diameter% wit! a
brig!t red edge and a dim red center wit! t!e a++earance of a K bulls eyeK 4n +!ysicale#am% t!e +atient !ad a fever of 3B. o C and an irregular !eart beat &er w!ite blood count
reflected an elevation of w!ite blood cells% greater t!an 1.%000 cells@cm3 ,!e +!ysician
ordered a test for :ymes Aisease to confirm !is sus+icions
1. *!at is t!e evidence suggests t!at t!e young graduate student !as contracted:ymes Aisease=
2. *!at is t!e environmental +at!way for t!is vector borne infection=
3. &ow did t!e student come into contact wit! t!is vector=4. *!at is t!e basis of t!e blood test t!at confirms t!e +resence of t!is organism=
. *!at are t!e c!aracteristics of t!is +at!ogen= $Mor+!ology% bioc!emistry% and
+at!ogenicity(
6. *!at +recautions s!ould t!e graduate student !ave used to avoid t!is disease=
7. ,!e sym+toms suggest w!at ty+e of antibiotic t!era+y=
8. &ow s!ould t!e treatment be followed to eliminate t!e +ossibility of serious and
continuing !ealt! +roblems=
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:I/'S
!tt+?@@wwwcdcgov@ncidod@dvbid@lyme@> CAC :yme Aisease
!tt+?@@wwwnlmni!gov@medline+lus@lymedisease!tml> Med:ine
!tt+?@@wwwlymenetorg@> :yme Aisease /etwork
!tt+?@@wwwmedicinenetcom@lymeFdisease@article!tm> Medicine net
!tt+?@@te#tbookofbacteriologynet@:yme!tml> ,odars ,e#tbook of Microbiology
!tt+?@@wwwwadswort!org@databank@borreli!tm> *adswort! Center
http://www.cdc.gov/ncidod/dvbid/lyme/http://www.nlm.nih.gov/medlineplus/lymedisease.htmlhttp://www.lymenet.org/http://www.lymenet.org/http://www.medicinenet.com/lyme_disease/article.htmhttp://textbookofbacteriology.net/Lyme.htmlhttp://www.wadsworth.org/databank/borreli.htmhttp://www.cdc.gov/ncidod/dvbid/lyme/http://www.nlm.nih.gov/medlineplus/lymedisease.htmlhttp://www.lymenet.org/http://www.medicinenet.com/lyme_disease/article.htmhttp://textbookofbacteriology.net/Lyme.htmlhttp://www.wadsworth.org/databank/borreli.htm8/13/2019 New Case Study Booklet-1
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Case Study Si#
,!e ,em+erature ,ells t!e ,ale
7ased U+on
Microbiology> Aiseases of t!e Aigestive System > ,ortora
Many of t!e ".00 students and em+loyees of a +ublic sc!ool system develo+ed diarr!ealillness on May "% "00G ,!e cafeteria served c!icken on t!at day 4n May 1% +art of t!e
c!icken was +laced in water>filled +ans and cooked in an oven for " !ours at a dial setting of1GGo C ,!e oven was turned off and t!e c!icken was left overnig!t in t!e warm oven ,!e
remainder of t!e c!icken was cooked for " !ours for a steam cooker and t!en left in t!edevice overnig!t at t!e lowest +ossible setting% -3oC ,wo seroty+es of a gram> negative%
cytoc!rome o#idase negative% lactose>negative rods were isolated from 3" +atients
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!tt+?@@wwwu!mcsunysbedu@microbiology@2R)ML"0/2),IE!tm> 2ram negative
enteric +at!ogens
!tt+?@@wwwbuddycomcom@bacteria@gnr!tml> Clinical bacteria
!tt+?@@vmcfsanfdagov@Jmow@c!a+1!tml> 7ad 7ug 7ook
!tt+?@@wwwcdcgov@ncidod@dbmd@diseaseinfo@salmonellosisFg!tm> CAC reference for
Salmonella
!tt+?@@wwwcdcgov@ncidod@dbmd@diseaseinfo@salmentFg!tm> Salmonella reference +age
!tt+?@@wwwsalmonellaorg@> ,!e latest u+dates on Salmonella
!tt+?@@wwwmicrobesinfo@resources@2eneralFMicrobiology@Microorganisms@ubacteria@Sal
monella@> Salmonella links
http://www.uhmc.sunysb.edu/microbiology/GRAM%20NEGATIVE.htmhttp://www.buddycom.com/bacteria/gnr.htmlhttp://vm.cfsan.fda.gov/~mow/chap1.htmlhttp://vm.cfsan.fda.gov/~mow/chap1.htmlhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/salmonellosis_g.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/salment_g.htmhttp://www.salmonella.org/http://www.microbes.info/resources/General_Microbiology/Microorganisms/Eubacteria/Salmonella/http://www.microbes.info/resources/General_Microbiology/Microorganisms/Eubacteria/Salmonella/http://www.uhmc.sunysb.edu/microbiology/GRAM%20NEGATIVE.htmhttp://www.buddycom.com/bacteria/gnr.htmlhttp://vm.cfsan.fda.gov/~mow/chap1.htmlhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/salmonellosis_g.htmhttp://www.cdc.gov/ncidod/dbmd/diseaseinfo/salment_g.htmhttp://www.salmonella.org/http://www.microbes.info/resources/General_Microbiology/Microorganisms/Eubacteria/Salmonella/http://www.microbes.info/resources/General_Microbiology/Microorganisms/Eubacteria/Salmonella/