Centre of Preventive Doping Research
Next Generation Gene Doping
Myths and Facts
Patrick Diel
Center of Preventive Doping Research
Dept. Molecular and Cellular Sports Medicine
German Sports University Cologne
Centre of Preventive Doping Research
Next Generation Gene Doping
Myths and Facts
Patrick Diel
Center of Preventive Doping Research
Dept. Molecular and Cellular Sports Medicine
German Sports University Cologne
Centre of Preventive Doping Research
Physical performance
Sceletal muscle
OxygenEnergy
Anabole Steroids EPO PPARs
Cognitive Abilitys
Methylphenidat
Zürich 2017
Centre of Preventive Doping Research
Zürich 2017
Centre of Preventive Doping Research
Doping is defined as the
occurrence of one or more of the
anti-doping rule violations set forth
in Article 2.1 through
Article 2.8 of the Code.
Zürich 2017
Centre of Preventive Doping Research
?Zürich 2017
Centre of Preventive Doping Research
Zürich 2017
Centre of Preventive Doping Research
Genes and Sport
Eero Mäntyranta: Mutation in the Epogene results in extraordinary physical
performance
Analysis of the
induividual genetic
predisposition to
increase physical
performance
Gene Analysis
Manipulation of the
gene activity to
increase physical
performance
Gendoping
Zürich 2017
Centre of Preventive Doping Research
Zürich 2017
Centre of Preventive Doping Research
Gene doping = Gene therapy ?
Zürich 2017
Centre of Preventive Doping Research
Gene doping vs. Gene therapyGene doping vs. Gene therapy
Gene therapy:
• Aim, Treatment of Diseases
• Risk and Befit must be in a balance
Gene doping:
• Aim, Increase of physical performance.
• Normally healthy powerful people are treated
• It is easier to increase performance than tu cure a life-threatening disease
• Doping is illegal, ethical aspects are not relevant
Zürich 2017
Centre of Preventive Doping Research
What is the difference between gene doping and doping ?
The issue of definition taking hematocrit as an example
Old fashion: Blood transfusion
Old fashion : recombinant EPO
The future: Modulation of endogenous Gene activity (HIF stabilizer's).
The future : Gene therapy?
Reality: Drug development based on gene therapy
is stopped. To risky to expensive , not efficient.
Zürich 2017
Centre of Preventive Doping Research
Moreover according to the
Wada definition the following
techniques also can be
defined to be gene doping
• Every kind of stem cell therapy
• siRNA techniques
• Techniques involving epigenetic modifications
• CRISPR / Cas9 techniques
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Centre of Preventive Doping Research
Doping NewsSalbutamol
Trimetazidine
Meldonium
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Centre of Preventive Doping Research
New drugs and therapies : the real challenge
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Centre of Preventive Doping Research
Zürich 2017
Centre of Preventive Doping Research
Myostatin
Zürich 2017
Centre of Preventive Doping Research
Sceletal muscle dystrophy
Metabolic Syndrome
Sarkopenia
Injurie treatment
Animal breeding
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Centre of Preventive Doping Research
Multiple Ascending Dose Phase 1 Study - A study in healthy postmenopausal women (A031-02), evaluating multiple doses of ACE-031
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Centre of Preventive Doping Research
Myostatin Inhibition
Chemical modification of DNAGen KO Termination of
transkription
siRNA
Blocking
Antibody
(Wyeth)
Overexpression of
Follistatin
Rezeptor
antagonists
Manipulation of the
processing
Application of
rekombinat propeptide
/AG Research)
Soluble ACTII B
(Acelleron)
Zürich 2017
Centre of Preventive Doping Research
Identifying doping abuse
Direct detection of
abused drug or
metabolites
Indirect detection by
biomarker analytics
Zürich 2017
Centre of Preventive Doping Research
Zürich 2017
Centre of Preventive Doping Research
Zürich 2017
Centre of Preventive Doping Research
Zürich 2017
Centre of Preventive Doping Research
Conclusions:
•The identification of new doping relevant molecular targets, the increase in molecular and cellular techniques and finally the introduction of new therapies and drugs, let to an exponential
increase in the complexity of doping.
•This is the real great challenge in the future fight against doping.
•Gene doping as defined by WADA is only a very limited part of the problem
• Combination of established detection sytems with biomarker monitoring and new detection techniques is a promising strategy in the future fight against doping
Erlangen 22.07.2016
