Ni Microscope

8/12/2019 Ni Microscope

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Upright Microscope ECLIPSE Ni

Upright Microscope


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System expandability Nikons proprietary stratum structure enables ef cient system construction. The numerous accessories can be custom combined depending upon application.

Optical performance CFI Plan Apochromat objectives with chromatic aberration correction and high

transmission throughout a broad range of wavelengths Objectives with improved transmission in near IR wavelength range for multiphoton

excitation imaging

Design 3D ergo design combines functionality with sophistication.

Operability Ni-E: Motorized model with automatic changeover of observation methods and

adjustment of microscope accessories. Ni-U: Manual model with some motorized options. Most microscope controls can be operated with easy-to-reach buttons on the front of

the Ni-E.

The Ni series, the ultimate in upright biological microscope evolution, supports bioscience studies withenhanced basic performance and exible system expandability.The newly developed CFI Plan Apochromat (lambda) series objectives are the key to the seriesoptical performance. Nano Crystal Coat, with its ultra low refractive index, is employed for the rst timen microscope objectives, providing brighter, high-resolution and high-contrast microscopy images.

Nikons proprietary stratum structure, used and highly acclaimed for inverted research microscopes,s now incorporated in an upright microscope, allowing a combination of components to be installed.

Applications using laser and uorescent proteins, such as Kaede and PA-GFP, are possible with theaddition of a two-tiered uorescent unit and a photoactivation unit.The Ni series transcends the concept of conventional upright microscopes and expands the possibilitiesof advanced research in elds such as bioscience and medicine.

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See the evolution


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The Ni series microscopes meet all research demandsThese microscopes have three features: expandability of the system due to the stratum structurehat enables optical paths in two tiers; superior optical performance, as represented by the Plan

Apochromat series objectives; and high-speed motorized accessories. The Ni series is designed tomeet the needs of all advanced bioscience and medical research.

Microscopy images courtesy of:Left) Torsten WhitmannCenter) Michael W. DavidsonRight) Dr. Jennifer Waters and Adrian Salic


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Ni-E

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Expandable system broadens application possibilities

Photo-activation imaging (Ni-E/Ni-U)Research into the reactions and changes of stimulated cells has become popular in recent years. Nikon has developed anew photoactivation unit for upright microscopes, a rst in microscopy.

Ni-E Ni-U Flexible stratum structureThis structure allows two-layer mounting of a photoactivation unit with an epi- uorescence attachment.

Objectives for long-wavelength laserWith CFI Plan Apochromat objectives, chromatic aberration has been corrected up to 850 nm andtransmission improved in the long wavelength range, increasing accuracy and ef ciency of laser excitationat target site.

Multi-color uorescent imaging (Ni-E)Demand for multi-color uorescent imaging that uses newly developed uorescent proteins and uorescent r eagents isconstantly increasing. Nikon meets such needs with diverse functions and optical technologies.

High-speed motorized componentsThe higher speeds of the motorized excitation and barrier lter wheel and the motorized shutter enable

uick wavelength changes, reducing photobleaching of the specimen.t can be operated via easily accessible control buttons, increasing operation ef ciency.

CFI Plan Apochromat series objectivesTransmission and chromatic aberration correction have been improved throughout the wide range of visibleo near IR wavelengths, allowing use of various uorescent reagents. They provide bright, high-contrast,igh S/N (signal-to-noise) ratio multi-color uorescence images with almost no focus shift when used withny wavelength.

Mounted motorized excitation/barrierlter wheel

Mounted photoactivation unit

0

Complies withFDAperformancestandardfor laser products exceptfor deviations pursuanttoLaser NoticeNo.50,datedJune24,2007.

IEC /EN60825-1:2007 TotalPower 500mWMAX.CW400 700nm


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Ni-E

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Multiphoton imaging (Ni-E)Multiphoton microscopy in which long excitation wavelengths are used to allow less-invasive imaging of ever deeper areas ofcells is gaining popularity. The design of the Ni-E model is optimized for multiphoton imaging in both optical and mechanicalystems to meet t he demands of today.

Objective dedicated to multiphoton imagingCFI75 Apochromat 25xW MP objective features chromatic aberration correction over a broad wavelengthange from 405 to 950 nm, high NA (numerical aperture) (1.10) and longer working distance (2.0 mm), andompatibility with water immersion and water dipping.

High-sensitivity multiphoton detector (NDD)Newly developed episcopic NDD (non-descanned detector) unit incorporates a detector that ef cientlyenses weak signals from deep areas of live specimens. In combination with a diascopic NDD unit,ransmitted signals can be also detected.

Retrofittable focusing nosepiece mechanismMicroscopes can be modi ed by switching the focusing stage and focusing nosepiece, enabling xed-stageon guration to meet demands of experiments such asin vivo imaging.

Con guration of manual microscope Ni-U withmotorized epi- uorescence cube turret, epi-

uorescence attachment, motorized septuplenosepiece, digital camera DS-Qi1 and DS-Fi2, controlunit DS-L3, simple remote control pad, control box B

Con guration of motorized microscope Ni-E withmotorized quadrocular tilting tube, motorizedDSC zooming port, motorized ND lter, digitalcamera DS-Fi2, motorized XY stage and joystick,control unit DS-L3, control box A

Enhanced system expandability providesthe best solutionNi series accessories are segmented by function, allowing you to select required units and

exibly combine them to create lean and effec tive system con gurations.

System conguration for acquiring multi-stained specimen images The motorized epi- uorescence cube turret shutter, which helps to reduce photobleaching of specimens, is easily operated witha convenient remote control pad.Images are automatically acquired by controlling the motorized accessories, including the epi- uorescence cube turretaccording to the camera settings such as exposure time, camera gain, and time interval.This con guration is recommended for those who conduct mostly uorescent observations rather than bright eld observations.

System conguration for acquiring pathological specimen images Optimal brightness can be automatically adjusted with objective changeover, eliminating the need for manual adjustment.As operations for optical zooming can be controlled from the control unit DS-L3, images of the desired angle of eld canbe captured while maintaining image quality.

Mounted episcopic NDD unitand diascopic NDD unit


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Versatile microscopes meet all demands

Ni-U (manual model) Ergonomic tube and stage height adjustment mec hanism allowcomfortable viewing positions. Stratum structure and sturdy design improve expandability. Motorized nosepiece, motorized epi-fluorescence cube turret,motorized shutter can be utilized.

Ni-E (motorized model) High-precision motorized focusing Broad range of motorized accessories that can be used in combination. Observation methods can be c hanged at a simple push of a button. Stratum structure and sturdy design improve expandability.3D ergo design buttons with improved operability are located close together for speedy operations. Microscope settings in use can be verified on the display. Optimized for multiphoton excitation imagingTwo focusing mechanism options: focusing stage and focusing nosepiece

Manual and motorized modelsTo meet diverse user demands for operability, system expandability and motorized control, Nikon provides two Ni seriesmodels. The Ni-U, which has compatibility with some motorized accessories, is the manual model suitable for high-qualitymage observation and digital imaging. The Ni-E is a fully motorized model that is ef cient for experiments r equiring

comprehensive control of various devices, such as photoactivation units and confocal systems.

Ni-E provides fully motorized operations

Automatic adjustment with objective changeoverCondenser, aperture and eld diaphragm, and ND lter are automatically set to the optimalposition during objective changeover. In addition, stage XYZ travel amount per handlerotation and parfocal distance deviation correction are automatically adjusted.Microscope settings can also be manually adjusted.

Observation mode changeoverObservation methods such as bright eld, DIC, phase contrast and uorescence can bedesignated individual buttons, enabling changes to be made at the push of a button. This isparticularly useful when the same sample is imaged with multiple observation methods.

High-precision motorized focusingHigh-precision Z-focus incorporated by the Ni-E provides accurate height informationrequired for use with confocal laser microscopes. Individual coarse and ne focus knobsprovide enhanced ease of operation.

Ni-E con gured with a motorized epi- uorescence cube turretand motorized universal condenser

Ni-U con gured with an ergonomic binocular tube


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Lens Lens

Reflected light Reflected lightIncident light Incident light

Nano Crystal CoatThis anti-re ective coating that consists of nanometer-size particles is based onsemiconductor manufacturing technology and is also used for Nikon camera lenses.The coarse structure with particles arranged in a spongy construction with uniformspaces between them enables extremely low refractive indices.

Supreme optical performanceAs a light microscope manufacturer, Nikon has cultivated high technical capabilities and con dence. With its advancedechnologies extending from optical glass production t o lens design, fabrication, coating and processing, Nikon provides

unsurpassed optical performance.

CFI Plan Apochromat seriesWith remarkably high NA, greatly improved transmission in the long wavelengthange thanks to Nikon proprietary Nano Crystal Coat, and chromatic aberrationorrection over 435 to 850 nm, these objectives are ideally suited not only forright eld and DIC observations but also for uorescent observations. These lensesllow acquisition of bright and clear images at any wavelength for near-IR imagingnd multi-color uorescence imaging. Because bright images can be captured even

with a weak excitation light, damage to a specimen is minimized.

Water dipping objective lensesWith a long working distance and high NA, these objectives provide excellentransmission in near IR wavelength range. The axial chromatic aberration of 40x and0x objectives has been corrected to up to 850 nm, allowing high-resolution imagesf minute structures in thick specimens during IR-DIC observation.

The 25xW MP and 100x objectives feature high NA (1.1) and a long working distance

2.0 mm). With chromatic aberration corrected in the IR region, these objectives aredeal for multiphoton excitation observation. In addition, by employing a mechanismo compensate for the changes in spherical aberration that occur at differentemperatures and depths of observation points, clear images of areas deep within ahick specimen can be captured.

Uniformly bright illuminationThe y-eye lens is ideally suited to diascopic illumination optical systems.Uniform and bright illumination up to the view eld periphery is provided atany magni cation.

High-performance objective lens

Epi- uorescence observation

Phase contrast observation

DIC (Differential Interference Contrast) observation

Bright eld observation

Excellent image acquisition with all observation methods

Fluorescencefilter cube

Lens

Camera

Lightsource

Light absorbingmaterial

NDStray light

Technologies supporting the Ni series

Conventional multi-layer coatingWithout Nano Crystal Coat With Nano Crystal Coat

Nano Crystal Coat

Fluorescence noise eliminationNikons proprietary noise terminator mechanism is employed in the epi-

uorescence cube turret and lter cubes. The S/N ratio has been dramaticallimproved by thoroughly eliminating stray light in the lter cubes, allowingimages of weak uorescent signals to be captured with high contrast andbrightness.


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Digital imagingThe Ni series can be controlled in conjunction with a Digital Sight series digital camera, facilitatingffortless digital imaging. Images can be captured with a dedicated button on the microscope body,he touch panel on the camera control unit, or the soft ware GUI on a PC, depending on your preference.

Ultimate ease, speed and clarity in imaging

Digital Sight series digital camerasA wide array of models with high sensitivity, high resolution, high speeds and faithfulcolor reproduction are available to meet all research needs and purposes.

Confocal imagingThe Ni series is sturdy enough for the incorporation of a confocal scanner.The broad ux of light allows the capture of bright confocal images.

Image capture buttonmages can be acquired by simply pressing the image capture button located on the

microscope base.

Multiphoton confocal microscope A1R MP +

A1R MP+ is capable of high-speed multiphoton imaging up to 420 fps. TheNDD enables deep specimen imaging with high sensitivity. High-speed,accurate GFP/YFP unmixing is possible with NDD.

Confocal microscope A1R+/A1+

A1+ incorporates a high-de nition (up to 4096 x 4086 pixels) non-resonantscanner. A1R+ also incorporates a high-speed (up to 420 fps) resonantscanner, enabling simultaneous photo activation and imaging.

Confocal microscope C2 +

C2+ features a compact design and high operability, and it supports4-channel simultaneous acquisition. It provides various imaging such asimage stitching (large images).

Spectral imaging confocal microscopeA1Rsi+/A1si+/C2si+

By incorporating a spectral detector, a wide wavelength spectrum of320 nm can be acquired with a single scan. Closely overlapping spectra canbe unmixed cleanly with no crosstalk.

High-sensitivity cooled monochrome camera headDS-Qi1

The new CCD drive circuit reduces readout noise.The cooling mechanism reduces heat-induced noise,allowing the capture of weak uorescence signals.Furthermore, there is a high frame rate of up to48 fps and a high quantitative linearity within 2%.

Ultrahigh-resolution cooled color camera head DS-Ri1

Provides the 12.7-megapixel equivalent, 2200 TV linehigh de nition. Smooth live image display at max.32 fps makes focusing easy. Superior color reproduction capabilities allow recording of accuratespecimen colors. The cooling mechanism reducesheat-induced noise in uorescent images.

High-denition cooled color camera headDS-Fi1c

Equipped with a 5-magapixel CCD. The coolingmechanism reduces heat-induced noise, making itideal for uorescent image acquisition.

High-denition color camera headDS-Fi2

Equipped with a 5-magapixel CCD. Displays liveimages at max. 21 fps. Minute structures can becaptured in bright eld, phase contrast and DICobservations.

Integrated imaging softwareNikons imaging software NIS-Elements employs a single platform to allow stress-free

perations, from image capture through to comprehensive control of camera, microscope anderipheral devices, to image processing, measurement, data management and analysis.

Stand-alone control unit DS-L3tand-alone control unit DS-L3 allows the Digital Sight series digital camera to be setp and operated without a PC connection. It also enables image acquisition from a PChrough a network. The DS-L3 is rmware based, and therefore will not compromise youracilitys network.

Optimal imaging parameters for each observation method can be selectedusing icons and simple measurements can be taken.Camera, motorized microscope functions and peripheral devices can becomprehensively controlled.Microscope status data, such as objective lens, fluorescent filter cube andcondenser information, is automatically saved along with thecaptured image when a motorized or intelligent unit is attached.Operations can be controlled with a mouse or a touch panel operated bytouch or stylus pen.Required icons can be selected and laid out as desired.

Image capture button

amera/microscope control Microscope control Scene mode icons

Ni-E con gured with C2+


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Wide array of accessories nely segmented by function

Motorized changeover of optical paths is possible. Eyepieceinclination can be adjusted from 15 to 35.

Effective for applications that require highly accuratepositioning, such as photo-activation imaging and FISH.

High-speed motorized changeover of condenser lensesfor bright eld, phase contrast, DIC and simple dark eldobservations is possible.

Laser light photo activation and episcopic illumination arepossible. Both the photoactivation unit and the confocalsystem can be used with a single laser source.

Noise terminator provides high S/N ratio. Six lter cubescan be installed. Either an epi- uorescence attachment ora photoactivation unit can be attached.

A digital camera can be mounted on the camera port. Amotorized 0.6x - 2.0x zoom optical system is incorporated.

Makes control of motorized XY stage possible.

Barrier lter positions (7 positionsusing 25 mm lters)can be changed at a high speed of 0.2 sec. per position.

Objective magni cation is automatically saved along withthe captured image.

High-speed shutter control is possible. The shutter can beattached to diascopic and episcopic illumination systems.

Brightness is automatically optimized with the changeoveof the motorized nosepiece. Motorized adjustment ofdesired brightness is also possible.

In addition to motorized microscope operation, XYZ contof stage is possible with similar operational ease as that ofan actual microscope.

Excitation lter positions (8 positionsusing 25mm ltecan be changed at a high speed of 0.15 sec. per position.

Objective magni cation is automatically saved along withthe captured image. Built-in prism/analyzer plate slot.

Motorized operation of nosepiece, epi- uorescence cubeturret and shutter is possible.

Motorized quadrocular tilting tube (Ni-E)

Motorized XY stage (Ni-E)

Motorized universal condenser Dry (Ni-E)

Photoactivation unit (Ni-E, Ni-U)

Motorized epi-uorescence cube turret (Ni-E,Ni-U)

Motorized DSC zooming port (Ni-E)

Joystick for motorized stage (Ni-E)

Motorized barrier lter wheel (Ni-E)

Motorized septuple nosepiece (Ni-E, Ni-U)

Motorized shutter (Ni-E, Ni-U)

Motorized ND lter (Ni-E)

Ergo controller (Ni-E)

Motorized excitation lter wheel (Ni-E)

Motorized sextuple DIC nosepiece (Ni-E, Ni-U)

Simple remote control pad (Ni-U)

Elegant design with absolute comfort

Our design goal for the Ni series was to provide physical and mental comfortduring use. Straight lines and at surfaces that are traditionally employed inmicroscope design do not exist in nature, so they induce a feeling of tensionwhile seated in front of the microscope. The Ni series employs a modernorganic design in the microscope wing that adds a gentle beauty whileeducing stress during lengthy observations.

When designing the Ni series, Nikon collaborated with Akio Shindate,previously involved in the design of Nikon SLR cameras from the early stageof concept building. Through this collaboration with Shindate, who excelsat unique ideas and st ylish designs, Nikon has produced innovative newmicroscopes that combine function with beauty.

Design for functionThe Ni series embodies the basic spirit of a product design for minimizinguser fatigue.

3D ergo design for comfortable touch-type operation sButtons on microscope sides are angled to allow touch-type operationsduring observation.Easy to see displayMicroscope settings are shown on a display that can be easily viewedfrom the observation position.Small footprintSpace-saving microscope base assures larger work space.

Contrast between elegance and sharpnessThe white microscope wings feature an innovative and elegant curveddesign. In contrast, the rectilinear and black microscope base reflectsNikons image of high quality and high performance. This new design willlso be employed in all accessories and Ci serie s clinical and laboratory

microscopes, and will continue in future models.

Akio Shindate (Director, VO Corporation Ltd.) Based in the United Kingdom. After graduating from a U.S. university, workedfor a design company in London and participated in a great number ofinternational projects. Became independent and established VO Corporation in2000. Active in design of automobiles and electronic devices for clients mainlyin Japan, the United States and Europe.


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System diagram: Ni-E focusing stage type

D F CO 1.43 -1.20

JAPAN

D F CD 0. 95 -0. 80

JAPAN

0.20.40.60.81.01.21.4

A -AN.A=1.4

JAPAN

D - C O i l

JAPAN

JAPAN

Coarse

Fine

ExFine

XYSpeedConstant Speed

Z Speed

Coarse

FineExFine

+Y

-Y

-X +X

POWER

MIN . M AX .

POWER

I 0SUPERHIGH PRESSUREMERCURYLAMPPOWERSUPPLY

RUNTIME

IGNITION

POWERLAMPREADY

REPLACE BULBBEFORE THESPECIFIEDTIME

O I

MADE INJAPAN

LAMPHOURS

PUSH ON

POWERLIGHT

XENONPOWERSUPPLYXPS-100

0

D-FB

A1

*6

*1

*2

*7

*7

*5*5

*4

*2

*1

*1

*1Doublelayer ismaximumpossible.*2When usedwith doublelayer, J connectionshouldbe low layerand I connectionshouldbe upperlayer.Single J l isnot possible.*3Thesecubescanonlybeusedwith I connection.*4Whenboth I and J areconnected.*5Cannotbeusedwithdoublelayerstatus.*6Cannotbeusedwithdoublelayerstatus.*7Shouldbe attachedwhenused withboth Ni-PAUandConfo*8C2canonlybeusedwithsinglelayerstatus.

C-HGFIF15/30HGFiber

UN2Transformer100W

HalogenLampSocket100W

HMXLamphouse

C-FCLEpi-FlCollectorLens

QuartzEpi-FlCollectorLens

XenonPowerSupply75W

C-SHG1PowerSupplyforHG100W

XeLampSocket75W

XeLamphouseHMX-4


HgLamphouseHMX-4B


MercuryLampSocketS100WBL

HgLamphouseHMX-3B

C-HGFI/HGFIEHG PrecenteredFiberIlluminatorIntensilight

C-LHGFIHGLamp

C-HGFIBHG100WAdapterR

C-IAAnalyzerTube forFirst-orderRedCompensation

C-ISAAnalyzerTubeforSimple Polarization

C-EREyelevelRiser

D-ESSliderforReectedIllumination

NI-PAUNiPhotoactivation Unit

TI-SHAEP-IMotorizedShutterAdapterforInvertedEpi-FL

NI-EXW-EMotorizedExcitationFilterWheel

NI-SH-EMotorizedShutter

NI-SHAEP-UMotorizedShutterAdapterforUprightEpi-FL

NI-SHCSMotorizedShutterCable Short

D-FBExcitationBalancer

NI-FLEIEPI-FluorescenceAttachment

NI-BAW-EMotorizedBarrierFilterWheel

TI-ADICAnalyzerCube

D-FLEFilterCubefor ReectedIllumination

D-FLDFilterCubefor DarkeldIllumination

C-FLEpi-FilterCube

NI-FLT6-EMotorizedEpi-uorescenceCubeTurret

NI-FLT6-IIntelligentEpi-uorescenceCubeTurret

NI-FLT6Epi-uorescenceCubeTurret

NI-FAFL/DICAnalyzer

NI-ASTAnalyzerSliderStopper

DS-L3DSCameraControlUnit

USBCableNI-CTLAControlBoxA

NI-ERGNIErgoController

NI-SJJoystickforMotorizedStage

D-DPDICRotatablePolarizer

C-TPPolarizerforFirst-orderRedCompensation

C-SPPolarizerforSimplePolarization

NI-LHPrecenteredLamphouse

NIE-CBConnectorBox

NI-SHADIMotorizedShutterAdapterforDia-Illumination

NI-SHCSMotorizedShutterCableShort


NI-ND-EMotorizedNDFilter

ECLIPSE Ni-E

D-CDF DarkeldModule

D-CPH-1PHModuleD-CPH-2PHModuleD-CPH-3PHModule

D-CDICN1DRYDICModuleD-CDICN2DRYDICModuleD-CDICNRDRYDICModule

D-C2-4X AuxiliaryLensfor N2Position

NI-CALN12-4XAuxiliary LensforN1 Position

NI-CUDUniversalCondenserDry

D-CDICModuleOil

D-CUODICCondenserOil

C-CAchromatSwing-outCondenser1-100x

XLWDCondenser

C-CAbbeCondenserNA0.9

C-CAchromatCondenserNA0.9

C-CAchromat/AplanatCondenser

DarkeldCondenserforX/Y(Dry)

C-CSlideAchromatCondenser2-100x

DarkeldCondenserforX/Y(Oil)

NI-CUD-EMotorizedUniversalCondenserDry

NI-CHCondenserHolder

NI-SSRSubstageforRotatable/MotorizedStage

NIE-CSRR2RightHandleRotatableCeramic-coated

StagewithHolderNI-S-EMotorizedXY Stage

CFIUW10xM

CFIUW10x

C-CTCenteringTelescope

CFI15xCFI10xMCFI10xCFI12.5x

NI-RPSDSCSupportColumn

NI-RPSABDSCAdapterB

NI-RPSAADSCAdapterA

NI-RPZDSCZoomingPort forQuadrocularTube

NI-RPZ-EMotorizedDSCZoomingPort forQuadrocularTube

NI-TTQuadrocularTiltingTube

NI-TT-EMotorizedQuadrocularTiltingTube

C-TAQTubeAdapterforQuadrocularTube

DICSlider

D-LPLambdaPlate

C-ASAnalyzerSlider forFirst-orderRedCompensation

D-SAAnalyzerSliderforSimplePolarization

D-DAAnalyzerSliderforDIC CFI60

ObjectiveLens

NI-N7-EMotorizedSeptupleNosepiece

NI-ND6-EMotorizedDICSextupleNosepiece

NI-N7-IIntelligentSeptupleNosepiece

NI-ND6-IIntelligentDICSextupleNosepiece

D-ND6DICSextupleNosepiece

C-NASextupleNosepiecewithAnalyzerSlot

L-DICDIC Slider

L-NU5UniversalQuintupleNosepieceESD

L-NBD5BDQuintupleNosepieceESD

L-DIHCDICSlider HC

CFI60ObjectiveLens

CFILU PlanFluorEPIObjectiveLens

CFILUPlanFluorBDObjectiveLens

NIE-CAMContactArmNI-SAMStandardArm

NI-DRSDSCRotationStopper

Y-TV0.55TVTube

Y-TVTVTube

V-TPhotoAdapter

C-mountTVAdapterVM4x

C-mountTVAdapterVM2.5x

RelayLens1x

C-mountTVAdapter

TVZoomLens

RelayLens1x

C-mountAdapter0.55x

C-mountAdapter0.7xC-mount

TVAdapterA

C-mountTVAdapter0.35x,0.45x,0.6x

C-mountZoomingAdapter

ENG-mountZoomingAdapter

ENG-mountTVAdapter

ENG-mountTVAdapter0.45x,0.6xProjectionLens

PLI2x,PLI2.5x,PLI4x,PLI5x

C-mountCameraENG-mountCameraFX-III SeriesPhotomicrographicSystem

C-TEP2DSC PortforErgonomicBinocularTube

C-TE2ErgonomicBinocularTube

C-TTTrinocularTubeT

C-TFTrinocularTubeF

C-TBBinocularTube

*8C-TT-C Trinocular Tube

C2Adapter

A1Adapter

C2

AchromatSwing-outCondenser2-100x

C-NSextupleNosepiece

LUNosepieceAdapter

*3

2

3

4

2

3

4


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JAPAN

JAPAN

Coarse

Fine

ExFine

XYSpeedConstant Speed

ZSpeed

CoarseFineExFine

+Y

-Y

-X +X

D-FB

N 1 D r y

JAPAN

USBCable

A1

*1 *2

*5

*5

*2

*1

*1

*1Double layerismaximumpossible.*2Whenusedwithdoublelayer, J connectionshouldbelow

layerand I connectionshouldbe upperlayer.Single J layisnot possible.

*3Thesecubescanonlybeusedwith I connection.*4Cannotbeusedwithdoublelayerstatus.*5ShouldbeattachedwhenusedwithbothNi-PAUandConfoc

C-HGFIF15/30HGFiber

C-HGFI/HGFIEHGPrecenteredFiberIlluminatorIntensilight

C-LHGFIHGLamp

C-HGFIBHG100WAdapterR

D-ESSliderforReectedIllumination

NI-PAUNiPhotoactivation Unit

TI-SHAEP-IMotorizedShutterAdapter forInvertedEpi-FL

NI-EXW-EMotorizedExcitationFilterWheel


NI-SHAEP-UMotorizedShutterAdapterforUprightEpi-FL



NI-FLEIEPI-FluorescenceAttachment

NI-BAW-EMotorizedBarrierFilterWheel

TI-ADICAnalyzerCube

A1-FN1-IRAnalyzerBlockIR

D-FLEFilterCubefor ReectedIllumination


C-FLEpi-FilterCube




NI-FAFL/DICAnalyzer

NI-ASTAnalyzerSliderStopper

DS-L3DSCameraControlUnit

NI-CTLAControlBoxA

NI-ERGNIErgoController

NI-SJJoystickforMotorizedStage

NIE-CBConnectorBox

NI-SHADIMotorizedShutterAdapter forDia-Illumination



NI-RPSABDSCAdapterB

NI-RPSAADSCAdapterA

C-TAQTubeAdapterforQuadrocularTube

NIE-CAMContactArmNI-SAMStandardArm


Y-TV0.55TVTube

Y-TVTVTube

V-TPhotoAdapter

C-mountTVAdapterVM4x

C-mountTVAdapterVM2.5x

RelayLens1x

C-mountTVAdapter

TVZoomLens

RelayLens1x

C-mountAdapter0.55x

C-mountAdapter0.7xC-mount

TVAdapterA

C-mountTVAdapter0.35x,0.45x,0.6x



ENG-mountTVAdapter

ENG-mountTVAdapter0.45x,0.6xProjectionLens

PLI2x,PLI2.5x,PLI4x,PLI5x


C-TEP2DSC PortforErgonomicBinocularTube

*4C-TT Trinocular TubeT

*4C-TF Trinocular TubeF

C-TBBinocularTube

C2Adapter

A1Adapter

C2

NI-RPZDSCZoomingPort forQuadrocularTube

NI-RPZ-EMotorizedDSCZoomingPort forQuadrocularTube


NI-TT-EMotorizedQuadrocularTiltingTube

*4

CFIUW10xM

CFIUW10x

C-CTCenteringTelescope

CFI15xCFI10xMCFI10xCFI12.5x

C-TE2ErgonomicBinocularTube

NI-SSF Substagefor FocusingNosepiece

D-CDICN2DRYDICModule

D-CDICN1DRYDICModule

FN-PT4X4XSupportLens

FN-CLWDCondenser

NI-SH-DDishHolder

NI-S-E MotorizedXYStage

NI-SAMotorizedXYStage Adapter

FN-3PS2 FN1StandardStage

NI-3PSAAdapter forFN1 StandardStage

CFI60ObjectiveLens

DICSliderCFI75ObjectiveLens

ObjectiveSlider

ObjectiveHolder

DICSlider

NI-FNAFocusingNosepieceSub Arm

NI-AMSL Arm Spacer 74mm

NI-AMSS Arm Spacer 49mm

NI-PTPolarizerTurret

NI-LH PrecenteredLamphouse

NI-IRLH PrecenteredIRLamphouse

NI-FIAFiberIlluminationAdapter


NI-ND-EMotorizedNDFilter

ECLIPSE Ni-E

FN-IRPIRPloarizerFN-PPolarizer

Fiber Illuminator

*3

System diagram: Ni-E focusing nosepiece type


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System diagram: Ni-U

JAPAN

JAPAN

*7

*1

*1

*1

*8

*2

*2

*5

*5

*4

*8

*6*9 *6

*1 Doublelayer is maximumpossible.Upper layer accepts onlyNI-FLT6 whendoublelayer is used.

*2 Whenusedwithdoublelayer, J connectionshouldbelower layer andI connectionshouldbeupper layer.Single J layer is notpossible.

*3 Thesecubes canonlybeused with I connection.*4 Whenboth I and J areconnected.*5 DS-L3 canbeconnected directlywhenNi-CTLBis notused.*6 Cannotbeusedwithdouble layer status.*7 Cannotbeusedwithdouble layer status.*8 Shouldbeattachedwhen usedwithboth Ni-PAUandConfocal.*9C2 canonlybeusedwith singlelayer status.




D-CDF DarkeldModule

D-CPH-1 PH ModuleD-CPH-2 PH ModuleD-CPH-3 PH Module

D-CDICN1 DRYDICModuleD-CDICN2 DRYDICModuleD-CDICNRDRYDIC Module

D-C2-4XAuxiliaryLensfor N2 Position

NI-CALN1 2-4XAuxiliary Lensfor N1 Position

NI-CUDUniversalCondenser Dry

D-CDICModuleOil

D-CUODICCondenserOil

C-CAchromatSwing-outCondenser1-100x

XLWDCondenser

C-CAbbeCondenserNA0.9

C-CAchromatCondenserNA0.9

C-CAchromat/AplanatCondenser

DarkeldCondenserfor X/Y(Dry)

C-CSlideAchromatCondenser2-100x

DarkeldCondenserfor X/Y(Oil)

C-CPhaseContrastTurretCondenser

CFI UW10xM

CFI UW10x

C-CTCenteringTelescopeCFI 15xCFI 10xMCFI 10xCFI 12.5x


NI-RPSABDSCAdapter B

NI-RPSAADSCAdapter A

NI-RPZDSCZoomingPortforQuadrocular Tube


C-TAQTubeAdapter forQuadrocular Tube

NIU-CAM ContactArmNI-SAM StandardA rm


Y-TV0.55TV Tube

Y-TVTV Tube

V-TPhotoAdapter

C-mountTV AdapterVM4x

C-mountTV AdapterVM2.5x

RelayLens1x

C-mountTV Adapter

TV ZoomLens

RelayLens1x

C-mountAdapter0.55x

C-mountAdapter0.7xC-mountTV Adapter A

C-mountTV Adapter0.35x,0.45x,0.6x



ENG-mountTV Adapter

ENG-mountTV Adapter0.45x,0.6xProjectionLens

PLI 2x,PLI 2.5x,PLI 4x,PLI 5x


C-TEP2 DSCPortfor ErgonomicBinocular Tube

C-TE2

ErgonomicBinocular Tube

C-TT

TrinocularTubeT

C-TF

TrinocularTubeF

C-TB

BinocularTube

C-TT-C

TrinocularTube

C2Adapter

C2

UN2 Transformer100W

HalogenLampSocket100W

HMXLamphouse

C-FCL Epi-FlCollector Lens

QuartzEpi-FlCollector Lens

XenonPower Supply75W

C-SHG1Power Supplyfor HG100W

XeLampSocket75W

XeLamphouseHMX-4


HgLamphouseHMX-4B


MercuryLampSocketS 100WBL

HgLamphouseHMX-3B

C-HGFI/HGFIE HGPrecentered FiberIlluminator Intensilight

C-LHGFI HGLamp

C-HGFIBHG100WAdapter R

C-HGFIF15/30HGFiber

DS-L3 DSCamera ControlUnit

NI-SRCPSimpleRemoteControlPadNI-CTLB

ControlBoxB

C-CTCCameraTrigger CableU3/L3

C-CTCCameraTrigger CableU3/L3

TI-100WRC100WLamphouseRemoteCable

D-DPDICRotatablePolarizer

C-TPPolarizerfor First-orderRedCompensation

C-SPPolarizerfor SimplePolarization

NI-LH PrecenteredLamphouse

NI-SHADI MotorizedShutter Adapter forDia-Illumination

NI-SHCL MotorizedShutter CableLong

NI-SH-EMotorizedShut ter

ECLIPSE Ni-U

NI-CHCondenser Holder

NI-SSRSubstagefor Rotatable/MotorizedStag e

NIU-CSRR2RightHandleRotatableCeramic-coatedStagewithHolder

C-H1L SpecimenHolder 1LC-H2L Specimen

Holder 2L

NI-SS Substage

C-CSR1S RightHandle Ceramic-coatedStagewith1S Holder

C-SR2SRightHandleStagewith2S Holder

C-CSRRightHandle Ceramic-coatedStage

D-ES Slider forReected Illumination

NI-PAUNiPhotoactivationUnit

NI-SH-EMotorizedSh utter

NI-SHAEP-UMotorizedShutter Adapterfor UprightEpi-FL

NI-SHCLMotorizedShutte rCableLong


NI-FLEI EPI-FluorescenceAttachment

NI-FAFL/DICAnalyzer

NI-ASTAnal yzerSlider Stopper

C-EREyelevelRiser

C-ISAAnalyzer TubeforSimplePolarization

C-IAAnalyzer TubeforFirst-order RedCompensation

Y-THPL LEDPointer Unitfor TeachingHead

Y-IM MagnicationMo dule

Y-THF TeachingUnitFacetoFace

Y-THS TeachingUnitSidebySide UnitB

TH-AC2 ACAdapter Y-THM MainTeachingUnit

Y-THRTeachingUnitSidebySide A

Y-THSPSupportfor SidebySide

AchromatSwing-outCondenser2-100x

DICSlider

D-LPLambda Plate

C-AS Analyzer Slider forFirst-order RedCompensation

D-SAAnalyzer Sliderfor SimplePolarization

D-DAAnalyzerSlider for DIC CFI60

ObjectiveLens

L-DICDICSlider

L-NU5 UniversalQuintupleNosepieceESD

L-NBD5 BDQuintupleNosepieceESD

L-DIHCDICSlider HC

CFI60ObjectiveLens

LUNosepieceAdapter

CFI LUPlanFluorEPI ObjectiveLen s

CFI LUPlanFluor BDObjectiveLens

*3TI-ADICAnalyzerCube

D-FLE FilterCubefor ReectedIllumination


C-FL Epi-Filter Cube

NI-N7-EMotorizedSeptupleNosepiece

NI-ND6-EMotorizedDICSextupleNosepie ce

NI-N7-I IntelligentSeptupleNosepiece

NI-ND6-I IntelligentDICSextupleNosepiece

D-ND6 DICSextupleNosepiece

C-NASextupleNosepiecewithAnal yzer Slot

C-NSextupleNosepiece

2

3

4

2

3

4


13/15

Speci cations

24 25

Ni-ENi-U

Focusing stage type Focusing nosepiece type

Main body

Optical system CFI60 in nity optical system CFI60 and CFI75 in nity optical systems CFI60 in nity optical system

Focusing (stroke from focuspoint)

Via motorized stage Up/Down movement (Up 2 mm/Down 13 mm)

Via motorized nosepiece Up/Down movement (Up 13 mm/Down 2 mm)

Via manual stage Up/Down movement (Up 3 mm/Down 26 mm)Built-in linear encoder, Resolution: 0.025 m

Motorized escape and refocus mechanism

Coaxial Coarse/Fine focusing

Illumination

Halogen lamp (12V100W) NI-ND-E Motorized ND Filter (option) Halogen lamp (12V100W)

Built-in y-eye lens Built-in NCB11, ND8, ND32 lters (detachable, one additional lter mountable) and diffuser (non-detachable)

Controls

Transmitted light On/Off switch, Intensity control dial with preset function Image capture button

Built-in motorized control switches

Stand-alone control unit DS-L3 NI-ERG NI Ergo Controller (option)

Stand-alone control unit DS-L3 (option) NI-SRCP Simple Remote Control Pad

(option)

Power supply unit External power supply NI-CTLA Control Box A for all con gurations

Built-in for halogen lampNI-CTLB Control Box B is necessarywhen Motorized/Intelligent options arecombined.

Eyepieces (F.O.V. mm) CFI 10x (22) CFI 10xM Photomask (22) CFI 12.5x (16)

CFI 15x (14.5) CFI UW10x (25) CFI UW10xM Photomask (25)

Tubes

F.O.V. 22 mm (Eyepiece/Port)

C-TB Binocular Tube C-TE2 Ergonomic Binocular Tube (100/0, 50/50 with C-TEP2 DSC Port)

Inclination angle: 10-30 degree, Extension up to 40 mm

F.O.V. 25 mm*1 (Eyepiece/Port)

C-TF Trinocular Tube F (100/0, 0/100) C-TT Trinocular Tube T (100/0, 20/80, 0/100) C-TT-C Trinocular Tube (100/0, 0/100, for confocal)*2 NI-TT Quadrocular Tilting Tube (Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0/0/100)

Inclination angle: 15-35 degree

NI-TT-E Motorized Quadrocular Tilting Tube(Eyepiece/Upper port/Rear port: 100/0/0, 0/100/0, 0 /0/100) Inclination angle: 15-35 degree

Arms NI-SAM Standard Arm

NIE-CAM Contact Arm (for Motorized/Intelligent options) NIU-CAM Contact Arm (for Motorized/Intellig ent options)

Nosepieces

Motorized NI-N7-E Motorized Septuple Nosepiece

NI-ND6-E Motorized Sextuple DIC Nosepiece

NI-N7-E Motorized SeptupleNosepiece

NI-ND6-E Motorized Sextuple DIC Nosepiece

Intelligent NI-N7-I Intelligent Septuple Nosepiece

NI-ND6-I Intelligent Sextuple DIC Nosepiece NI-N7-I Intelligent Septuple Nosepiece NI-ND6-I Intelligent Sextuple DICNosepiece

Manual

D-ND6 Sextuple DIC Nosepiece

C-N Sextuple Nosepiece

C-NA Sextuple Nosepiece with Analyzer Slot L-NU5 Universal Quintuple Nosepiece ESD L-NBD5 BD Quintuple Nosepiece ESD

FN-S2N Sliding Nosepiece (for CFI60 objectives) Changeover 2 objectives, DIC slider insertable

FN-MN-H Single Objective Holder (for CFI75 objective) DIC slider insertable

D-ND6 Sextuple DIC Nosepiece C-N Sextuple Nosepiece

C-NA Sextuple Nosepiece withAnalyzer Slot L-NU5 Universal Quintuple Nosepiece ESD L-NBD5 BD Quintuple Nosepiece ESD

Stages

NIE-CSRR2 Right Handle Rotatable Ceramic- coated Stage with 2S Holder

Cross travel 78(X) x 54(Y) mm Handle height and torque adjustable

FN-3PS2 FN1 Standard Stage Cross travel 30(X) x 30(Y) mm

C-SR2S Right Handle Stage with 2S Holder C-CSR1S Right Handle Ceramic-coatedStage with 1S Holder

C-CSR Right Handle Ceramic-coated Stage NIU-CSRR2 Right Handle Rotatable Ceramic-coated Stage with 2S Holder

Cross travel 78(X) x 54(Y) mm Handle height and torque adjustable

NI-S-E Motorized XY Stage Resolution: 0.1 m

NI-SH-D Dish Holder (option)

Substages NI-SSR Substage (for Motorized Universal Condenser andRotatable/Motorized stages)

NI-SSF Substage for Focusing Nosepiece(for LWD condenser and FN1 Standard/Motorized

stages)

NI-SSR Substage (for Rotatable stage) NI-SS Substage (for Non-rotatablestages)

Ni-ENi-U

Focusing stage type Focusing nosepiece type

Condensers

Motorized

NI-CUD-E Motorized Universal Condenser Dry For DIC, phase contrast, dark eldobservationsAttached on NI-SSR Substage

Manual

NI-CUD Universal Condenser Dry C-C Abbe Condenser NA 0.9 C-C Achromat Condenser NA 0.9 Dark eld Condenser (Oil) Dark eld Condenser (Dry) C-C Achromat/Aplanat Condenser NA 1.4 C-C Slide Achromat Condenser 2-100x C-C Achromat Swing-out Condenser 1-100x Achromat Swing-out Condenser 2-100x X LWD Condenser D-CUO DIC Condenser Oil

FN-C LWD Condenser NA 0.78 (for DIC and oblique light illumination)

NI-CUD Universal Condenser Dry C-C Abbe Condenser NA 0.9 C-C Achromat Condenser NA 0.9 Dark eld Condenser (Oil) Dark eld Condenser (Dry) C-C Achromat/Aplanat CondenserNA 1.4

C-C Slide Achromat Condenser 2-100 C-C Achromat Swing-out Condenser1-100x

C-C Phase Contrast Turret Condenser*3 Achromat Swing-out Condenser 2-100 X LWD Condenser D-CUO DIC Condenser Oil

Epi-uorescence

illuminator

Filter cube turret

6 lter cubes mountable, High S/N noise terminator mechanism for all turrets NI-FLT6-E Motorized Epi- uorescence Cube Turret

Motorized shutter, Status check function*4 NI-FLT6-I Intelligent Epi- uorescence Cube Turret

Manual shutter, Status check function*4 NI-FLT6 Epi- uorescence Cube Turret Manual shutter

Light distributiondevice

NI-FLEI Epi- uorescence Attachment Aperture diaphragm and eld diaphragm (Centerable/Detachable), ND lters (ND4, ND8, ND16)

NI-PAU Ni Photoactivation Unit (405 nm to 650 nm lasers)

Option

NI-BAW-E Motorized Barrier Filter Wheel 7 lters mountable, 0.2 sec between adjacent positions

NI-EXW-E Motorized Excitation Filter Wheel 8 lters mountable, 0.15 sec between adjacent positions

NI-SH-E Motorized Shutter 0.012 sec between open and close state

NI-SH-E Motorized Shutter 0.012 sec between open and close stat

Epi-illumination light source

C-HGFI/HGFIE HG Precentered Fiber Illuminator Intensilight (130W) Hg Lamphouse and Power Supply (100W)*2 Xe Lamphouse and Power Supply (75W)*2 Halogen Lamphouse and Transformer (100W)*2

Power consumption211W(with max. halogen lamp intensityand full motorized options)

96W (with max. halogen lamp intensityand full motorized options)

Main body: 133W (with max. halogenlamp intensity) Control Box B: 29W (with full motorizoptions)

Weight (approx.)29 kg(Epi- uorescent con guration with motorizedquadrocular tilting tube)

42 kg(Photoactivation con guration with motorizedquadrocular tilting tube)

20 kg(Bright eld con guration with ergonombinocular tube)

*1 When used with a double layer, such as with layered epi- uorescence cube turrets, F.O.V. is 22.*2 Cannot be used with the focusing nosepiece type.*3 Can only be mounted on the NI-SS Substage.*4 Status check function: Status of Filter/Nosepiece etc. can be recorded with captured images and/or displayed on the controller monitor.


14/15

Dimensional diagram

130

6 0

2 0 5

437

6 0 4

320

3 8 9

A.S

JAPANFN-S2N

0

Compl ieswithFDA performancestandardforlaser products exceptfordeviations pursuanttoLaserNoticeNo.50,datedJune24, 2007.

IEC/EN60825-1:2007

Total Power 500mWMAX.CW400700nm

320 383

2 0 5

3 4 0

6 0

4 8 8

130

A

320 383

2 0 5

3 4 0

6 0

130

5 0 1

26 27

Ni-E (for use with focusing nosepiece)Con gured with an Ni photoactivation unit, two-tieredmotorized epi- uorescence cube turret and motorizedquadrocular tilting tube

Ni-UCon gured with a motorized epi- uorescence cube turretnd quadrocular tilting tube

Ni-UCon gured with an ergonomic binocular tube and DSC port

Unit: mm


15/15

Specifications and equipment are subject to change without any notice or obligation on the partof the manufacturer. November 2011 2011 NIKON CORPORATION

Company names and product names appearing in this brochure are their registered trademarks or trademarks.N.B. Export of the products* in this brochure i s controlled under the J apanese Foreign Exchange and Foreign Trade Law.

Appropriate export procedure shall be required in case of export from Japan.*Products: Hardware and its technical information (including software)Monitor images are simulated.

WARNINGTO ENSURE CORRECT USAGE, READ THE CORRESPONDINGMANUALS CAREFULLY BEFORE USING YOUR EQUIPMENT.

NIKON CORPORATION

Shin-Yurakucho Bldg., 12-1, Yurakucho 1-chome, Chiyoda-ku, Tokyo 100-8331, Japanphone: +81-3-3216-2375 fax: +81-3-3216-2385http://www.nikon.com/instruments/

NIKON INSTRUMENTS INC.1300 Walt Whitman Road, Melville, N.Y. 11747-3064, U.S.A.phone: +1-631-547-8500; +1-800-52-NIKON (within the U.S.A. only)fax: +1-631-547-0306http://www.nikoninstruments.com/

NIKON INSTRUMENTS EUROPE B.V.Laan van Kronenburg 2, 1183 AS Amstelveen, The Netherlandsphone: +31-20-44-96-300 fax: +31-20-44-96-298http://www.nikoninstruments.eu/

NIKON INSTRUMENTS (SHANGHAI) CO., LTD.CHINA phone: +86-21-6841-2050 fax: +86-21-6841-2060(Beijing branch) phone: +86-10-5831-2028 fax: +86-10-5831-2026(Guangzhou branch) phone: +86-20-3882-0552 fax: +86-20-3882-0580

NIKON SINGAPORE PTE LTDSINGAPORE phone: +65-6559-3618 fax: +65-6559-3668

NIKON MALAYSIA SDN. BHD.MALAYSIA phone: +60-3-7809-3688 fax: +60-3-7809-3633

NIKON INSTRUMENTS KOREA CO., LTD.KOREA phone: +82-2-2186-8400 fax: +82-2-555-4415

NIKON CANADA INC.CANADA phone: +1-905-602-9676 fax: +1-905-602-9953

NIKON FRANCE S.A.S.FRANCE phone: +33-1-4516-45-16 fax: +33-1-4516-45-55

NIKON GMBHGERMANY phone: +49-211-941-42-20 fax: +49-211-941-43-22

NIKON INSTRUMENTS S.p.A.ITALY phone: +39-055-300-96-01 fax: +39-055-30-09-93

NIKON AGSWITZERLAND phone: +41-43-277-28-67 fax: +41-43-277-28-61

NIKON UK LTD.UNITED KINGDOM phone: +44-208-247-1717 fax: +44-208-541-4584

NIKON GMBH AUSTRIA AUSTRIA phone: +43-1-972-6111-00 fax: +43-1-972-6111-40NIKON BELUX BELGIUM phone: +32-2-705-56-65 fax: +32-2-726-66-45

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