NIST Traceable UV/Vis/NIR Reference Sets

SPRING 2015

NIST TraceableUV/Vis/NIR Reference Sets

ISO/IEC-17025 Accredited ISO Guide 34

USB Drive supplied with each set contains the reference certificate and all vital information required

Starna Cells, Inc (800) 228-44822

UKAS ISO/IEC 17025 Accredited / NIST Traceable

Construction of reference materials

the following purposes:* Demonstration of satisfactory instrument performance to any

inspection or regulatory agency.* Detection of instrument malfunction.* Provide data to a service technician allowing detection of

instrument faults and assistance in repair.Each spectrophotometer has specific operating characteristics based on the geometry and basic design of the optical system. These reference materials become your guide to the efficient operation of your instrument.

Ultraviolet, visible and NIR spectrophotometers are fundamentally both accurate and precise but it is vital to check instrument performance on a regular basis to ensure operation is within satisfactory parameters. References provided by Starna are based on materials, which have been used or produced by a National Metrology Laboratory (NMI), such as the National Institute of Standards and Technology (NIST) or fundamental metrological principles. When used in routine testing of instrument performance, a quality control database of the operating characteristics for the specific instrument can be built. This database can then be used for

NIST (National Institute for Standards and Technology in Washington DC, USA) traceability is available on all our reference materials, unless otherwise indicated. Traceability is established during the calibration of each of our five reference spectrophotometers using NIST primary standards, both before and after calibrating Starna reference materials. Documentation supplied with Starna references details the analysis procedure, NIST traceability, the analyzed values of the reference and the confidence limits. Certification supplied with Starna reference materials is suitable for use with all inspecting agencies when

used in the context of standard operating procedures for quality control, established in your laboratory. Our calibration laboratory is inspected and accredited by UKAS under ISO/IEC 17025 for the Starna calibration laboratory and under ISO Guide 34 as a reference material producer. This level of accreditation assures the user that the certification of our reference materials is of the highest standard and may be used to validate instrument performance in all ISO/IEC 17025 regulated environments.

Starna has over forty-five years of experience pioneering the technology of sealing reference materials into quartz spectrophotometer cells. The technology allows the use of materials in the UV range where Glass Filters are not usable. The sealed reference cells have the same optical characteristics as cells used in normal analysis thus removing

many variables, which might be introduced using any other form of optical configuration or reference preparation. The references have a lifetime guarantee subject to specified conditions and are supplied in suitable storage containers. The Glass Filters are made to the highest industry standards with raw material of known characteristics.

What makes Starna reference materials unique?All sealed, Glass Filters or Metal on Quartz reference materials produced by Starna are “Ready to Use”. References are supplied in either heat fusion sealed quartz cells or, in the case of the Glass or Metal on Quartz Filters, in an aluminum holder with the same external dimensions as a standard spectrophotometer cell.

Starna's sealed references are environmentally friendly as they eliminate the need to open an ampoule, use it once and throw it away. Wet chemistry methods are no longer required to make up the references from raw materials. With care,

Properties of Starna heat fusion sealed Quartz cells, glass or Metal on Quartz references:1) Sealed cells ensure cleanliness, repeatability and reliability2) They can be used multiple times over many years3) The range covers the Far UV, UV, Visible and NIR4) All calibrated & NIST traceable references can be recertified.

Should I use NIST Traceable References?Traceability to NIST allows for a definitive, certifiable method of analysis of the reference using the same methods as NIST. Additionally, each reference raw material is either purchased from NIST (where available) or directly tested against a NIST supplied reference. The most important element of NIST traceability is the Starna certificate of analysis and traceability.

This certificate details the method of analysis and the certified results of the analysis. You can use these certified results as the basis of your quality program. If your lab is routinely monitored by a certifying agency, then the Starna level of accreditation and NIST traceability is invaluable.

the sets will last for many years. All NIST traceable sets can be recertified.

Why do you need to use reference materials?

www.starnacells.com 3

Ultraviolet ReferencesMaterial: Used for: Traceable to: Configuration: Page(s):Potassium Dichromate Absorbance, Linearity, UV SRM 935a Filled Quartz Cell 4, 5, 6 & 7Nicotinic Acid Absorbance, Far UV SRM 935a Filled Quartz Cell 8 & 9Metal on Quartz Filters Absorbance, Linearity, UV/VIS SRM 2301 Metal Coated Quartz Window 10Starna Green Absorbance, Wavelength, Linearity UV/VIS SRM 935a/2034 Filled Quartz Cell 11Sodium Chloride Stray light Far UV SRM 2032 Filled Quartz Cell 18 & 19Potassium Chloride Stray light Far UV SRM 2032 Filled Quartz Cell 18 & 19Potassium Iodide Stray light UV SRM 2032 Filled Quartz Cell 18 & 19Sodium Iodide Stray light UV SRM 2032 Filled Quartz Cell 18 & 19Lithium Carbonate Stray light UV SRM 2032 Filled Quartz Cell 18 & 19Sodium Nitrite Stray light VIS SRM 2032 Filled Quartz Cell 18 & 19Acetone Stray light VIS SRM 2032 Filled Quartz Cell 18 & 19Holmium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 12Didymium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 13Samarium Oxide Wavelength UV/VIS SRM 2034 Filled Quartz Cell 14Rare Earth Oxide Wavelength Far UV SRM 2034 Filled Quartz Cell 15Benzene Vapor Resolution not controlled Filled Quartz Cell 20Toluene in Hexane Resolution SRM 935a Filled Quartz Cell 21

Visible ReferencesMaterial Used for Traceable to: Configuration: Page:Neutral Density Glass Filters Absorbance, Linearity, VIS SRM 930e Glass Filters 22, 23 & 25Holmium Glass Filter Wavelength VIS SRM 2034 Glass Filter 24 & 25Didymium Glass Filter Wavelength VIS SRM 2034 Glass Filter 24

Near InfraredMaterial Used for Traceable to: Configuration: Page:Metal on Quartz Filters Absorbance, Linearity, NIR SRM 2301 Metal Coated Quartz Window 16 NIR Wavelength material Wavelength, NIR SRM 2065 Filled Quartz Cell 17

DNA and RNA 260/280 Ratio ReferencesMaterial Used for Traceable to: Configuration: Page: Stable Aqueous 260/280 Absorbance UV SRM 935a Filled Quartz Cell 28Stable Aqueous 260/280 Absorbance UV SRM 935a Vials, Screw or Crimp top 29

Microplate Reader ReferencesMaterial Used for Traceable to: Configuration: Page:Glass Filters Wavelength, Absorbance, VIS SRM 2034, 930e Glass Filters 26Adaptor To facilitate the use of standard sealed quartz cells or glass filters to validate Microplate Readers 27

Fluorescent & Raman ReferencesMaterial Used for Traceable to: Configuration: Page:Quinine Sulphate Solution Wavelength, Intensity & Linearity SRM 2034, 930e Filled Quartz Cell 30Ultra High Purity Water Fluorescent Instrument Sensitivity Filled Quartz Cell 31

References for use with methods described in...Material Test Range USP Ph. Eur. DAB BP ASTM TGA

Holmium Oxide Wavelength UV/Visible √ √ √ √ √ √Toluene in Hexane Spectra Resolution UV √ √ √ √ √ √Potassium Chloride Stray Light UV √ √ √ √ √ √Sodium Iodide Stray Light UV √ √ √ √ √ √Potassium Iodide Stray Light UV √ √ Potassium Dichromate Absorbance UV √ √ √ √ √ √Neutral Density Filters Absorbance Visible √ √ √ √ √ √

Table of Contents:


Potassium Dichromate - UV - Absorbance/Linearity up to 1.5ADescription & NIST Traceability: Potassium Dichromate from NIST (SRM 935a), permanently heat fusion sealed in quartz

cells, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration.

Primary Usage: Testing absorbance and linearity scale in the UV region of the spectrum up to 1.5 Absorbance.

Certified Wavelengths: 235, 257, 313 and 350 nm for 20mg/L to 100mg/L. 430 nm for 600mg/L.Physical Configuration: UV quartz cells that have been permanently heat fusion sealed.

Potassium Dichromate solvated in dilute perchloric acid is deemed to be the best method for testing the absorbance scale and linearity of spectrophotometers in the UV. Solid Potassium Dichromate used by Starna is purchased directly from NIST (SRM 935a). Solutions are made up in accordance to NIST certification and then permanently heat fusion sealed in Far UV quartz spectrophotometer cells.

The filled cells are tested and certified against the expected values from NIST. A certificate is provided with each set of cells, which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for quality control, established in your laboratory. Each set is supplied with a sealed blank cell used to obtain the certified measurements.

Description Part Number, NIST Traceable Price Linearity Dichromate set:Potassium Dichromate, 5 concentrations & Blank Cell20, 40, 60, 80 & 100mg/L & Blank Cell RM-0204060810 $ 2130.00 Single Concentrations: Potassium Dichromate, 20 mg/L & Blank Cell RM-02 $ 830.00 Potassium Dichromate, 40 mg/L & Blank Cell RM-04 $ 830.00 Potassium Dichromate, 60 mg/L & Blank Cell RM-06 $ 830.00 Potassium Dichromate, 80 mg/L & Blank Cell RM-08 $ 830.00 Potassium Dichromate, 100 mg/L & Blank Cell RM-10 $ 830.00 Additional concentration required for European Pharmacopoeia Compliance: Potassium Dichromate, 600 mg/L RM-60 $ 830.00 Linearity Dichromate set, with additional concentration for European Pharmacopoeia Compliance:Potassium Dichromate, 6 concentrations & Blank Cell RM-020406081060 $ 2450.00

Scan the absorbance of the Potassium Dichromate cells over the certified wavelength range, against the blank supplied.

Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget† of the certified references into consideration, if the absorbance values fall within the expected parameters† of your instrument then your instrument is working correctly.

To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the four wavelengths.

If you determine that your instrument is not giving you the correct values, consult your service technician for advice on how to determine and correct any problem, which this reference may have detected.

†For expanded uncertainty budget and expected parameters see page 35

The following chart is a listing of approximate absorbance values for each concentration based on the wavelength at which the Potassium Dichromate cells are to be read:

Concentration 235 nm 257 nm 313 nm 350 nm 430 nm20 mg/L 0.243 0.281 0.095 0.209 n/a40 mg/L 0.492 0.572 0.192 0.426 n/a60 mg/L 0.741 0.862 0.289 0.634 n/a80 mg/L 0.996 1.159 0.385 0.853 n/a100 mg/L 1.243 1.448 0.480 1.069 n/aEuropean Pharmacopoeia Compliance requires the following additional reference:

600mg/L n/a n/a n/a n/a 0.950

Product Description:

Suggestions for Use:

Ordering Information:


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Potassium Dichromate Solutions

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20 mg/l

Potassium Dichromate - UV - Absorbance/Linearity up to 1.5A

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Dichromate Concentrations

Potassium Dichromate Linearity

257 nm

235 nm

350 nm

313 nm

Linearity:Plotting concentration against absorption using the set of 5 cells against the blank, you can test your spectrophotometer for linearity through the UV range.

The use of the NIST traceable set with 5 different concentrations and a blank is recommended for best results.

Spectral scan:The graph to the left is a scan of 10 different concentrations of Potassium Dichromate. It clearly shows the peaks and troughs in relation to absorbance and the differences due to Potassium Dichromate concentration.

Peaks: 257 and 350 nmTroughs: 235 and 313 nm

The certificate supplied with the NIST traceable sets lists the absorbance values for each peak and trough at the four wavelengths measured against the perchloric acid blank at the four wavelengths. When measured in your spectrophotometer you can validate your transmittance/absorbance scale for accuracy and linearity using the set with 5 different concentrations and the blank.

RM-0204060810 set:

This NIST traceable set of one blank and five increasing concentrations of Potassium Dichromate is our most useful and widely purchased set consisting of 6 cells with the following materials:

Cell# Containing Concentration1 Perchloric acid blank 0.001N2 Potassium Dichromate 20 mg/L3 Potassium Dichromate 40 mg/L4 Potassium Dichromate 60 mg/L5 Potassium Dichromate 80 mg/L6 Potassium Dichromate 100mg/L


Potassium Dichromate - UV - Absorbance/Linearity up to 3.3ADescription & NIST Traceability: Potassium Dichromate from NIST (SRM 935a), permanently heat fusion sealed in quartz

cells, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration.

Primary Usage: Testing absorbance and linearity scale in the UV region of the spectrum up to 3.3 Absorbance.

Certified Wavelengths: 235, 257, 313 and 350 nm for 20mg/L to 240mg/L. Physical Configuration: UV quartz cells that have been permanently heat fusion sealed.

Potassium Dichromate solvated in dilute perchloric acid is deemed to be the best method for testing the absorbance scale and linearity of spectrophotometers in the UV. Solid Potassium Dichromate used by Starna is purchased directly from NIST (SRM 935a). Solutions are made up in accordance to NIST certification and then permanently heat fusion sealed in Far UV quartz spectrophotometer cells.

The filled cells are tested and certified against the expected values from NIST. A certificate is provided with each set of cells, which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for quality control, established in your laboratory. Each set is supplied with a sealed blank cell that was used to obtain the certified measurements.

Description Part Number, NIST Traceable Price Linearity Dichromate set from 1.5 to 3.5A Potassium Dichromate, 6 concentrations & Blank Cell120, 140, 160, 180 & 220 & 240mg/L & Blank RM-121416182224 $ 2450.00 Linearity Dichromate set from 0.19 to 3.5APotassium Dichromate, 6 concentrations & Blank Cell40, 80, 120, 160, 200 & 240 mg/L & Blank RM-040812162024 $ 2450.00 Customer specified Potassium Dichromate Concentration options, including those up to 1.5A from page 4: Potassium Dichromate, 1 concentration & Blank $ 830.00 Potassium Dichromate, 2 concentrations & Blank $ 1155.00 Potassium Dichromate, 3 concentrations & Blank $ 1480.00 Potassium Dichromate, 4 concentrations & Blank $ 1805.00 Potassium Dichromate, 5 concentrations & Blank $ 2130.00Potassium Dichromate, 6 concentrations & Blank $ 2450.00 Potassium Dichromate, 7 concentrations & Blank $ 2775.00

Scan the absorbance of the Potassium Dichromate cells over the certified wavelength range against the blank supplied.


To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the four wavelengths.


The following chart is a listing of approximate absorbance values for concentrations 120mg/l or higher, based on the wavelength at which the Potassium Dichromate cells are to be read:

Concentration 235nm 257 nm 313 nm 350 nm 120 mg/L 1.501 1.749 0.582 1.290 140 mg/L 1.758 2.050 0.679 1.506160 mg/L 2.016 2.351 0.777 1.723180 mg/L 2.275 2.655 0.875 1.940200 mg/L 2.536 2.960 0.974 2.157220 mg/L 2.790 3.256 1.071 2.373 240 mg/L 3.043 3.552 1.168 2.589



Ordering Information:†For expanded uncertainty budget and expected parameters see page 35


Potassium Dichromate - UV - Absorbance/Linearity up to 3.3A

Linearity:Plotting concentration against absorption using the set of 5 cells against the blank, you can test your spectrophotometer for linearity through the UV range.

The use of the NIST traceable set with 5 different concentrations and a blank is recommended for best results.

Spectral scan:The graph to the left is a scan of 10 different concentrations of Potassium Dichromate. It clearly shows the peaks and troughs in relation to absorbance and the differences due to Potassium Dichromate concentration.

Peaks: 257 and 350 nmTroughs: 235 and 313 nm

The certificate supplied with the NIST traceable sets lists the absorbance values for each peak and trough at the four wavelengths measured against the perchloric acid blank at the four wavelengths. When measured in your spectrophotometer you can validate your absorption scale for accuracy and linearity using the set with 5 different concentrations and the blank.

RM-121416182224 set:

This NIST traceable set of one blank and six increasing concentrations of Potassium Dichromate is our most useful and widely purchased set consisting of 7 cells with the following materials:

Cell# Containing Concentration1 Perchloric acid blank 0.001N2 Potassium Dichromate 120 mg/L3 Potassium Dichromate 140 mg/L4 Potassium Dichromate 160 mg/L5 Potassium Dichromate 180 mg/L6 Potassium Dichromate 220 mg/L7 Potassium Dichromate 240 mg/L

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Potassium Dichromate Solutions

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Dichromate Concentrations

Potassium Dichromate Linearity

257 nm

235 nm

350 nm

313 nm


Description & NIST Traceability: Nicotinic Acid, Far UV Absorbance standard, sealed in quartz cells, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Testing absorbance scale and linearity in the Far UV region of the spectrum.

Certified Wavelengths: 213 nm, 261 nm

Physical Configuration: UV quartz cells that have been permanently heat fusion sealed

The use of Nicotinic Acid solvated in dilute hydrochloric acid is a well documented method for the validation of the absorbance scale and linearity of a spectrophotometer in the Far UV region. When prepared in 0.1M hydrochloric acid, Nicotinic Acid gives a spectral scan containing characteristic peaks at approx. 210 nm and 260 nm. Within the concentration range 5-25 mg/l, if the absorbance scale of a narrow SBW (2 nm) spectrophotometer is linear, the apparent absorbances of a series of concentrations will be a linear function of concentration, at a specified SBW.

The filled cells are tested and certified against the calculated values. A certificate is provided with each set of cells which has the certified absorbance values for each cell and the confidence limit. The certificate supplied is suitable for use with inspecting agencies when used in the context of a standard operating procedure for the quality control that

Description Part Number, NIST Traceable Price Linearity Nicotinic Acid set:Nicotinic Acid, 4 concentrations & Blank Cell RM-1A2A3A4A $ 1980.00 Single Concentrations: Nicotinic Acid , 6 mg/L & Blank Cell RM-1A $ 880.00 Nicotinic Acid, 12 mg/L & Blank Cell RM-2A $ 880.00 Nicotinic Acid, 18 mg/L & Blank Cell RM-3A $ 880.00 Nicotinic Acid, 24 mg/L & Blank Cell RM-4A $ 880.00

is established in your laboratory. Each set is supplied with a sealed blank cell that was used for the certified measurement.

The following chart is a listing of approximate absorbance values for each concentration based on the wavelength at which the Nicotinic Acid cells are to be read at 1 nm SBW*:

Concentration 213 nm 261 nm

6 mg/L 0.243 0.281

12 mg/L 0.492 0.572

18 mg/L 0.741 0.862

24 mg/L 0.996 1.159

* Alternative SBW values available on request.

Nicotinic Acid - Far UV - Absorbance/Linearity




Scan the absorbance of the Nicotinic Acid cells over the certified wavelength range against the blank supplied.


To evaluate instrument linearity, plot a graph of concentration against absorbance for each of the two wavelengths.




Nicotinic Acid - Far UV - Absorbance/Linearity

Far UV Linearity Assessment:By plotting concentration against absorption when measuring the set of 4 Nicotinic Acid cells against the blank, you can test your spectrophotometer for linearity through the Far UV range.

Spectral scan:The graph to the left is a scan of a set of the 4 different concentrations of Nicotinic Acid. It shows the peaks clearly in relation to absorbance and the differences due to Nicotinic Acid concentration.

Peaks: 213 and 261 nm

The certificate supplied with these NIST traceable references shows the absorbance values for each peak when measured against the hydrochloric acid blank, at the two wavelengths. When measured in your spectrophotometer you can validate your transmittance/absorbance scale for accuracy and linearity using the set with 4 different concentrations and the blank.

RM-1A2A3A4A set:

This NIST traceable set of one blank and four increasing concentrations of Nicotinic Acid is unique in its ability for the verification of the Far UV absorbance scale. It consists of 5 cells with the following materials:

Cell# Containing Concentration

1 Hydrochloric acid blank 0.1M 2 Nicotinic Acid 6 mg/L 3 Nicotinic Acid 12 mg/L 4 Nicotinic Acid 18 mg/L 5 Nicotinic Acid 24 mg/L

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Nicotinic Acid Solutions

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Nicotinic Acid Linearity

213nm

261nm


The Starna RM-1Q3Q9Q is a direct equivalent to the NIST SRM 2031, with a standard range of Metal on Quartz absorbance standards for the UV/Visible area of the spectrum.

All Starna Metal on Quartz Filters have an optically contacted Quartz Cover Plate to protect the metalised surface and facilitate easy cleaning. This was also a feature of the original NIST SRM 2031.

The filters allow Absorbance and Linearity at the ten wavelengths across the range and the relative values are shown on the table below. They can be certified at wavelengths other than those shown above, on request. The filters are produced in our ISO 9001 accredited production facility, under ISO Guide 34 as a reference material producer. Calibration is carried out in our ISO/IEC 17025 accredited Calibration Laboratory.

Metal on Quartz UV/Vis Absorbance/LinearityDescription & NIST Traceability: Metal on Quartz Filter for absorbance and linearity in the UV and Visible area of the

spectrum. NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Certified Wavelengths: 250, 280, 340, 360, 400, 465, 500, 546.1, 590 and 635 nm at Spectral Band Width of 5 nm or less.

Physical Configuration: Metal coated quartz filter with coated side protected by an optically contacted quartz window. The filters are mounted in protective aluminum holders that will fit a standard instrument cell holder.

Description Part Number, NIST Traceable Price

Metal on Quartz Reference, 30%, & Blank Holder RM-3Q $ 1290.00Metal on Quartz Reference, 3%, 50%, & Blank Holder RM-Q35Q $ 1890.00Metal on Quartz Reference, 10%, 30%, 90%, & Blank Holder RM-1Q3Q9Q (NIST 2031a) $ 2850.00

The UV/Visible Metal on Quartz Filters have a relatively flat profile across all UV/Visible wavelengths. Normally three representative absorption filters would be chosen for any given application but the filters may be bought individually, in sets as shown below or any combination from the standard range.

Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget† of the certified references into consideration, if the

absorbance values fall within the expected parameters† of your instrument then your instrument is working correctly.

It is important to confirm that Metal on Quartz Filters are suitable for use with your instrument as they have a highly reflective surface and some instruments are sensitive to back reflection within the sample compartment.

Available Metal on Quartz Filters: Part No. %T Transmittance Absorbance Q1 1% 2.00 A Q3 3% 1.52 A 1Q 10% 1.00 A 3Q 30% 0.52 A 5Q 50% 0.30 A 9Q 90% 0.06 A

RM-1Q3Q9Q (SRM 2031 Equivalent)

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Metal on Quartz

Q1

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9Q



Ordering Information (Let us know what combination you require):



Starna Green Broadband - Wavelength, Absorbance/Linearity


Starna Green, Blank, 25X concentration RM-1SG $ 770.00Starna Green, Blank, 50X concentration RM-2SG $ 770.00Starna Green, Blank, 75X concentration RM-3SG $ 770.00Starna Green, Blank, 100X concentration RM-4SG $ 770.00Starna Green, Blank, 4 concentrations RM-1SG2SG3SG4SG $ 1720.00

Description & NIST Traceability: A mixed ultra stable dye solution permanently heat fusion sealed in quartz cells. NIST traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Testing wavelength accuracy, absorbance scale and linearity in UV/Vis region.

Certified Wavelengths: 257, 416, and 630 nm with a SBW up to 12 nm

Physical Configuration: Far UV quartz cell that has been permanently heat fusion sealed.

The use of stable aqueous dye solutions is an established and well recognized method for the validation of the absorbance scale and linearity of a spectrophotometer.

When prepared at the appropriate concentrations, these references have spectral characteristics that allow both the certification of the wavelength (257, 416, and 630 nm) and absorbance scale (0.25, 0.5, 0.75 or 1.0 A) in one discrete reference. Within the concentration range available, if the

absorbance scale of the spectrophotometer is linear, the apparent absorbencies of a series of concentrations will be a linear function of concentration.

The Starna Green reference set of proven stability is supplied with a Certificate of Calibration valid for 2 years by the Starna Calibration Laboratory, which is accredited by UKAS under ISO/IEC 17025, and ISO Guide 34.

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Starna Green

4S - 100

3S - 75

2S - 50

1S - 25

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Metal on Quartz

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Holmium Oxide - UV/Vis - Wavelength ReferenceDescription & NIST Traceability: Holmium oxide (4%) in perchloric acid (10%) sealed in a quartz cell, NIST Traceable

complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Verification of wavelength scale accuracy in both UV and Visible

Certified Wavelengths: 241.0, 249.9, 278.2, 287.1, 333.5, 345.6, 361.4, 385.4, 416.1, 451.1, 467.8, 485.2, 536.5, 640.6 nm

Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2, and 3 nm. Other SBWs can be provided on request.

Physical Configuration: Filled Far UV quartz cell that has been permanently heat fusion sealed


Holmium, Wavelength reference cell, NIST traceable RM-HL $ 550.00

Holmium has been used as a wavelength standard for many years in both a doped glass filter and in liquid format. Starna has extended the use by creating a filled and permanently heat fusion sealed far UV quartz cell which allows it to be used into the UV.

Offering sharp, stable peaks over the range of 240 to 650 nm Holmium perchlorate is used to confirm that the wavelength scale of your instrument is within the manufacturer's tolerances for the actual wavelength being measured.

The use of the liquid filled quartz cell ensures that the optical configuration method for quality control is exactly the same as for a normal analysis.

Holmium presents a wide range of sharp resolvable peaks, which are easily used to compare the wavelength indication on your spectrophotometer to the known peak. The first procedure should be to scan the cell over the usable range to assure that all peaks can be resolved. Adjust the slit width (SBW) if possible and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances of the wavelength readout. If not, a service technician may

be required to check or adjust your instrument. Holmium is measured against air as a blank makes no difference to the certified values. It is not recommended or good practise to evaluate absorbance using a wavelength reference.

Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Holmium reference will enable the user to build a data log of the instrument’s spectral accuracy for use with certification and troubleshooting should the correlation change over time.




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Holmium Oxide Solution


Didymium Oxide - Visible - Wavelength ReferenceDescription: Didymium Oxide in perchloric acid, sealed in a quartz cell, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Verification of wavelength scale accuracy in the Visible spectrum

Certified Wavelengths: 298.0, 329.0, 354.0, 444.1, 468.7, 481.8, 512.0, 521.3, 574.9, 731.8, 740.1, 794.1, 801.2, 865.0 nm

Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2 and 3 nm. Other SBWs can be provided on request.

Physical Configuration: Filled far UV quartz cell that has been permanently heat fusion sealed


Didymium, Wavelength reference cell, NIST traceable RM-DL $ 770.00

Didymium is a combination of two chemical elements, Praseodymium and Neodymium and has been used as a visible wavelength standard in the form of doped Glass Filters.

Starna provides a quartz cell filled and permanently heat fusion sealed with a Didymium Perchloric solution that will facilitate more effectively the evaluation of your instrument.

Didymium offers sharp, stable peaks over the range of 290 to 870 nm. The primary use of Didymium is to confirm that the wavelength scale of your instrument is within the manufacturer's tolerances for the actual wavelength being measured.


Didymium presents a wide range of sharp resolvable peaks, which are easily used to compare the wavelength indication on your spectrophotometer to the known peak. The first procedure should be to scan the cell over the usable range to assure that all peaks can be resolved. Adjust the slit width (SBW) if possible and scan rate, to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances of the wavelength readout. If not, a service technician may

be required to check or adjust your instrument. Didymium is measured against air as a blank makes no difference to the certified values. It is not recommended or good practise to evaluate absorbance using a wavelength reference.

Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Didymium reference will enable the user to build a data log of the instrument’s spectral accuracy for use with certification and troubleshooting should the correlation change over time




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Samarium Oxide - UV/Vis - WavelengthDescription & NIST Traceability: Samarium Oxide in perchloric acid, sealed in a quartz cell, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Verification of wavelength accuracy in both UV/Visible, resolving complex peaks

Certified Wavelengths: 235.2, 266.7, 279.1, 280.2, 305.3, 317.6, 331.7, 344.6, 401.5, 415.2 441.2, 463.5 478.5 nm


Physical Configuration: Far UV quartz cell that has been permanently sealed by heat fusion


Samarium Oxide, Wavelength reference, NIST Traceable RM-SL $ 770.00

Samarium Oxide in a perchlorate solution is a particularly good reference for establishing wavelength calibration because it has useful peaks from 230 to 560 nm. Many peaks have a natural bandwidth of less than 5 nm permitting precise location of the peaks.

This offers a distinct advantage over Glass Filters, which may have natural bandwidths greater than 30 nm.

In addition to offering wavelength calibration, the Samarium oxide can be used to check instrument spectral bandwidth. Using peaks in the 230-240 nm or the 410-420 nm region, the instrument capability to resolve complex peaks can be tested.

Samarium presents a wide range of sharp resolvable peaks, which are easily used to compare the wavelength indication on your spectrophotometer to the known peak. The first procedure should be to scan the cell over the usable range to assure that all peaks can be resolved. Adjust the slit width (SBW) if possible and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances of the wavelength readout. If not, a service technician may

be required to check or adjust your instrument. Samarium is measured against air as a blank makes no difference to the certified values. It is not recommended or good practise to evaluate absorbance using a wavelength reference.

Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Holmium reference will enable the user to build a data log of the instrument’s spectral accuracy for use with certification and troubleshooting should the correlation change over time.




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Rare Earth Oxide - Far UV - Wavelength ReferenceDescription & NIST Traceability: Rare Earth Oxide (0.05%) in sulfuric acid (0.03%) sealed in a quartz cell, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Verification of the Far UV wavelength scale of spectrophotometers

Certified Wavelengths: 201, 212, 223, 240, 253 nm


Physical Configuration: Filled far UV quartz cell that has been permanently heat sealed


Far UV Wavelength Reference, NIST traceable RM-RE $ 850.00

The validation of the wavelength scale in the Far UV is difficult because of the lack of suitable reference materials.

Starna has developed a new Rare Earth reference material which allows wavelength scale validation down to 200 nm. The reference consists of a solution of the Rare Earth material dissolved in an aqueous solution of very dilute sulfuric acid, which has been permanently heat fusion sealed into a high quality quartz cuvette.


Each reference set is supplied with a NIST Traceable certificate, which indicates the expected wavelength of each of five peaks and the confidence limit of the analysis.

This Rare Earth reference presents a wide range of resolvable peaks, which are easy to compare with the wavelength indication on your spectrophotometer. The first procedure should be to scan the Rare Earth reference cell over the usable range to ensure that all of the peaks can be resolved. Adjust the slit width and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances for the wavelength of your spectrophotometer. If not, a service technician may be required to check or adjust your instrument.

Rare Earth is measured against air as a blank makes no difference to the certified values. It is not recommended or good practise to evaluate absorbance using a wavelength reference.

Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Rare Earth reference will enable the user to build a data log of the instrument’s spectral accuracy in the Far UV for use with certification and troubleshooting should the correlation change over time.




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A standard range of Metal on Quartz absorbance standards for the NIR area of the spectrum to facilitate full compliance with USP requirements.

All Starna Metal on Quartz Filters have an optically contacted quartz cover plate to protect the metalised surface and facilitate easy cleaning.

The filters allow absorbance and linearity at certified wavelengths across the range from 250 nm to 2800 nm and the relative values are shown on the table to the right. They can be certified at wavelengths other than those shown above, on request.

The filters are produced in our ISO 9001 accredited production facility under ISO Guide 34 as a reference material producer and calibrated in our ISO/IEC 17025 accredited Calibration Laboratory.


Metal on Quartz Near Infra Red (NIR) Absorbance/Linearity

Available Metal on Quartz NIR Filters: Part No. %T Transmittance Absorbance NQ1 1% 2.00 A NQ3 3% 1.52 A 1NQ 10% 1.00 A 3NQ 30% 0.52 A 5NQ 50% 0.30 A 9NQ 90% 0.06 A


Metal on Quartz NIR Reference, 30%, & Blank Holder RM-3NQ $ 1990.00Metal on Quartz NIR Reference, 3%, 50%, & Blank Holder RM-NQ35NQ $ 3090.00Metal on Quartz NIR Reference, 1%, 3%, 50%, Blank Holder RM-NQ1NQ35NQ $ 4220.00

RM-1NQ3NQ9NQ

Description & NIST Traceability: Metal on Quartz Filter for absorbance/linearity in the Near IR area of the spectrum. NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibrationCertified Wavelengths: 250.0, 360.0, 465.0, 546.1, 635.0, 1100, 1700, 2210, 2500, 2800 nmPhysical Configuration: Metal coated quartz filter with coated side protected by an optically contacted quartz

window. The filters are mounted in protective aluminum holders that will fit a standard instrument cell holder.

ISO Accredited: ISO/IEC 17025, ISO Guide 34 by UKAS & ISO 9001 by BSI.

The NIR Metal on Quartz Filters are intended for use across a broad spectrum including the NIR with a relatively flat transmission profile across all wavelengths. Normally three representative absorption filters would be chosen for any given application but, the filters may be bought individually, in sets as shown below or any combination from the standard range.

Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty

budget† of the certified references into consideration, if the absorbance values fall within the expected parameters† of your instrument then your instrument is working correctly.

It is important to confirm that Metal on Quartz Filters are suitable for use with your instrument as they have a highly reflective surface and some instruments are sensitive to the positioning of such materials in the sample holder.


Ordering Information (Let us know what combinations you require):


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Near Infrared - Wavelength ReferenceDescription & NIST Traceability: Quartz cell in either Transmittance or Transmittance/Transflectance format. NIST

Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibrationPrimary Usage: Verification of wavelength scale accuracy in the NIR spectrumCertified Wavelengths: 990.7, 1154.9, 1446.6, 1652.3, 1693.1, 1948.0, 2060.1, 2111.0, 2248.2, 2353.3, 2373.4, 2385.4, 2537.5 nmSpectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.5, 1, 1.5, 2 and 3 nm. Other SBWs can be provided on request.Physical Configuration: Filled near IR quartz cell that has been permanently heat sealed

Resolvable Peaks990.7 nm1154.9 nm1446.6 nm1652.3 nm1693.1 nm1948.0 nm2060.1 nm2111.0 nm2248.2 nm2353.3 nm2373.4 nm2385.4 nm2537.5 nm

The reference is used to validate the wavelength scale of a NIR spectrophotometer. With 13 certified peaks from 990 to 2537 nm that include some complex for instrument resolution evaluation. The reference is available in two formats:

Transmittance: - 10mm optical pathlength with two clear windows (opposite windows to allow light to pass through the cell).

Transmittance/Transflectance - 5mm or 10mm optical path length, depending on orientation. In 5mm configuration, the rear window is mirror coated to provide reflectance optical return so pathlength is effectively 10mm ( 2 x 5mm = 10mm).

Each reference set is supplied with full certification and traceability to NIST through SRM-2065. The reference material is produced and certified in our ISO Guide 34 and ISO/IEC 17025 accredited laboratory. The reference can also be recertified as required.


NIR Wavelength Reference, Transmittance, NIST Traceable RM-NIR $ 1,290.00NIR Wavelength Reference, Transmittance and Transflectance, NIST Traceable RM-NIR/T $ 1,480.00

Transmittance Transmittance/Transflectance



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Stray Light Reference MaterialsDescription & NIST Traceability: Materials with sharp cutoffs in transmission at specified wavelengths, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Detection of stray light in the UV and Visible

Usable Range: 200 nm to 390 nm, depending on the material

Physical Configuration: Filled far UV quartz cell that has been permanently heat fusion sealed

Stray light is an indication of light transmitted through the sample, when there should not be light being transmitted. The presence of more stray light than specified in the instrument operator's manual may cause errors in your analysis.

Stray light can be a problem over any wavelength range but the problem increases the further into the UV that instrument measurements are taken.

The stray light reference materials are intended to determine the presence of stray light in your instrument. Each material stops the transmission of light below a specified wavelength. Any indication of light transmission below the specified “cutoff” wavelength by definition, must be stray light.

Testing for stray light is important even if the spectrophotometer is not used below 390 nm, because it is an excellent indication of the overall health of the instrument optics, grating and deuterium lamp. Stray light determinations are run against a water blank supplied with all NIST traceable stray light references. The stray light calibration certificate is measured against the water blank supplied with the reference cell. When there are more than one stray light references in a set, they are all calibrated against a single stray light blank cell.

The procedure for using the stray light references is similar for all materials. Set your spectrophotometer’s wavelength 20 nm above the cutoff for the stray light reference that you are using (for Potassium Iodide you would start at 280 nm). Insert the stray light reference cell in the measurement cell holder and the stray light blank cell in the blank cell holder of your instrument. Scan down into the UV to the lowest wavelength that your instrument manual specifies. Any light transmitted

below the cutoff wavelength will be stray light. If the amount of stray light is greater than the specification given in your instrument manual, a service technician may be required to investigate and correct the problem.

Periodically rescan with the same instrument configuration and compare the results. Over time you will have a data trail for your instrument which will make the detection and correction of any problems relating to stray light much more reliable.

Description Part Number, NIST Traceable Price per set

Sodium Nitrite & Blank Cell RM-SN $ 810.00Potassium Iodide & Blank Cell RM-KI $ 810.00Sodium Iodide & Blank Cell RM-SI $ 810.00Lithium Carbonate & Blank Cell RM-LC $ 810.00Potassium Chloride & Blank Cell RM-KC $ 810.00Sodium Chloride & Blank Cell RM-SC $ 810.00Acetone RM-AC $ 780.00

Material: Cutoff: Concentration:

Sodium Nitrite 390 nm 5% aqueousPotassium Iodide 260 nm 1% aqueousSodium Iodide 260 nm 1% aqueousLithium Carbonate 227 nm saturatedSodium Chloride 205 nm 1% aqueousPotassium Chloride 200 nm 1.2% aqueousAcetone 326 nm Spectroscopy grade





Stray Light Reference Materials

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Benzene Vapor for Spectral ResolutionDescription: 0.1ml Benzene in a vapor state sealed in a quartz cell

Primary Usage: Test the resolution at various bandwidths of grating based instruments in the UV

Usable Range: 270 nm to 230 nm

Physical Configuration: Far UV quartz cell that has been permanently heat fusion sealed

The ability of your spectrophotometer to resolve absorption peaks is the basis of its theory of operation. If absorption peaks cannot be resolved adequately, then interfering peaks will be integrated with the correct peaks, thus giving an inaccurate measurement.

Benzene vapor is an effective material for the detection of a grating based spectrophotometer’s resolving power, as it has a great number of close but distinct absorption peaks.

Benzene vapor may not work well with a photodiode array spectrophotometer.

Set your spectrophotometer to a Spectral Band Width (SBW) and a scan rate and note the settings. Scan from 240 to 265 nm and compare the peaks to the two charts and determine what changes need to be made to the setting of your spectrophotometer to improve the resolution of the scan. Continue to alter the slit width until you have optimized

the resolution. Make a note of the settings in your quality procedures.

Each time that the scan is repeated you can compare the new scan with previous scans to check for any variance. If your instrument needs service because of a drop in resolution, the historical data will greatly assist the service technician.

Description Part Number Price

Instrument Resolution reference cell, Benzene Vapor RM-BZ $ 450.00




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Benzene Vapour Spectra at various Spectral Bandwidths (SBWs) - offset for clarity

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1.0 nm SBW


Ultraviolet Bandwidth- Toluene in Hexane

The Spectral Bandwidth (SBW) of a spectrophotometer is the basis of establishing its ability to resolve spectral features separated by small differences in wavelength. If a sample to be measured has an absorption peak at 257.4 nm and there is an interfering peak at 260.0 nm then it should be established whether or the spectrophotometer is able to resolve the two peaks separately or “mixes” the two absorption peaks into a single peak, leading to erroneous results.

Toluene in Hexane is used as a reference for the calculation of SBW. The ratio of the transmittance/absorbance of the solution when read at two peak wavelengths (~268.7 and ~267.0 nm) relates directly to the SBW of the instrument being assessed. Regular use of this technique will assure that the resolution of your instrument is within the required range for your work.

The Toluene in Hexane reference consists of two far UV quartz spectrophotometer cells with the solution permanently sealed by heat fusion in the cells. One cell contains 0.02% Toluene in Hexane, the other is a Hexane only blank. The Toluene in Hexane cell is traceable to SRM 935a which is a transmittance/absorbance reference material.

Description & NIST Traceability: Quartz cell filled with Toluene in Hexane and permanently heat fusion sealed, NIST traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Determination of bandwidth and resolution in the UV regionUsable Range: 267.0 nm and 268.7 nmPhysical Configuration: Far UV quartz cells that have been permanently sealed by heat fusion


Toluene in Hexane cell with blank, NIST traceable RM-TX $ 860.00

Table of Approximate Ratios

Ratio: 2.5 2.1 1.6 1.4 1.0SBW: 0.5 1.0 1.5 2.0 3.0



270269

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Neutral Density FiltersDescription & NIST Traceability: Neutral Density Glass Filters for absorbance/linearity in the Visible area of the spectrum. NIST traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Validation of the visible of photometric scale of spectrophotometers.

Certified Wavelengths: 440.0, 465.0, 546.1, 590.0, 635.0 nm

Physical Configuration: Glass Filters mounted in anodized aluminum holder

The Starna neutral density filter sets are based on and traceable to the NIST sets SRM 930e, SRM 1930 and SRM 2930. These sets are used to test the photometric scale in both Transmission and Absorbance in the visible wavelength range.

The filters are made from Schott NG-type glass, which block a known percentage of the light passing through it. We calibrate the glass type and thickness of the filters to produce known transmittance and absorbance values. The approximate values are shown on the chart below. Each NIST traceable filter is individually tested and certified.

The neutral density filters are mounted in a black anodised aluminum holder, 12.5 x 12.5 x 45 mm, which is fully compatible with all standard spectro pho tom e ter cell hold ers. The filters have been shown most efficient in instruments with an effective bandwidth of 2.2 nm to 6.5 nm.

Set your spectrophotometer in either absorbance or transmission mode at 440.0 nm. Using the empty filter holder (blank) zero the instrument. Insert each filter in turn and note the value. Set your instrument to each successive wavelength and repeat the procedure. The value that you read should be close to the measured certificate value listed for that filter.


These readings from your instrument may also be used to plot a graph of the instrument linearity.

The user may also build a data log of the instrument’s spectral photometric accuracy. This can be used for certification and troubleshooting should the correlation change over time. If there is any discrepancy in the readings, a service technician can use the data collected to diagnose and correct any problems with your instrument.

Set RM-1N2N3NThis set is identical to NIST SRM 930e and consists of three filters and a blank holder. Approximate values are: %T Transmittance Absorbance 10% 1.10 A 20% 0.78 A 30% 0.56 A Set RM-N1N35NThis set is identical to NIST SRM 1930 and consists of three filters and a blank holder. Approximate values are: %T Transmittance Absorbance 1% 2.10 A 3% 1.60 A 50% 0.30 ASet RM-D1D39NThis set is identical to NIST SRM 2930 and consists of three filters and a blank holder. Approximate values are: %T Transmittance Absorbance 0.1% 3.00 A 0.3% 2.50 A 92% 0.04 A


Neutral Density Filter set, 10, 20, 30 %T, & Blank Holder RM-1N2N3N $ 1190.00 Neutral Density Filter set, 1, 3, 50 %T, & Blank Holder RM-N1N35N $ 1190.00Neutral Density Filter set, 0.1, 0.3, 92 %T, & Blank Holder RM-D1D39N $ 1190.00






Visible References - Neutral Density Filters

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Glass Wavelength Filter - Holmium, DidymiumDescription & Traceability: Holmium glass filter and Didymium glass filter, NIST traceable complete with UKAS ISO/

IEC 17025 accredited certificate of calibration

Primary Usage: Verification of wavelength scale accuracy in both UV and Visible

Usable Range: 241 nm to 640 nm (Holmium for UV/Vis). 430 nm to 890 nm (Didymium for Visible)

Spectral Band Width: The certificate indicates the above wavelength measured at 0.1, 0.25, 0.50, 1, 1.5, 2, and 3 nm. Other SBWs can be provided on request.

Physical Configuration: Stress free glass filter mounted in an anodized aluminium holder


Holmium Glass, Wavelength reference filter, NIST traceable RM-HG $ 350.00Didymium Glass, Wavelength reference filter, NIST traceable RM-DG $ 480.00

Both Holmium and Didymium provide distinctive peaks that make each suitable for use as a wavelength standard. These robust Starna filters are more suitable for some environments than the liquid cells. They are mounted in an anodized aluminum housing to protect the polished surfaces.

The Holmium and Didymium Glass Filters are used to ensure that the instrument wavelength scale is within the expected tolerances for the actual wavelength being measured.

Holmium and Didymium filters present a wide range of peaks that can be resolved. The number of peaks resolved will depend on the spectral bandwidth used. The wavelength peaks can then be used to compare the wavelength indicated by your spectrophotometer to the known peak.

The initial procedure should be to scan the filter over the usable range to identify all the peaks that can be resolved at the specified SBW of the instrument. Adjust the slit width (SBW)and scan rate to produce a usable spectra. Check each peak to ensure that the reading on your spectrophotometer is

within the manufacturer's tolerances of the wavelength readout. If not, a service technician may be required to check or adjust your instrument. The filters should be measured against an air blank.

Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Holmium or Didymium reference will enable the user to build a data log of the instrument’s spectral accuracy for use with certification and troubleshooting should the correlation change over time.




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RM-1N3N5DHG - Photometric Scale / WavelengthDescription & Traceability: Neutral Density Filter Set plus Holmium Glass Filter, NIST traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Validation of the visible of photometric scale and the UV/Vis wavelength scale

Usable Range: 440.0 to 635.0 nm (1N3N5D); 241 to 640 nm (Holmium for UV/Vis)

Physical Configuration: Glass Filters mounted in anodized aluminum holder

The elements of this set include neutral density filters that are used to test the photometric scale in both Transmission and Absorbance in the visible wavelength range. The filters are made from Schott NG-type glass which absorbs a known percentage of the light passing through it.

Adding the Holmium glass filter to the set facilitates validation of the wavelength scale by utilizing the distinctive peaks of the Holmium filter.

The thickness of the filters is calculated to produce known transmission and absorption values. The approximate values are shown on the chart. Each NIST traceable filter is individually tested and certified. The neutral density and Holmium filters are mounted in black anodized aluminum holders, 12.5 x 12.5 x 45 mm, which is fully compatible with all standard spec tro pho tom e ter cell hold ers. The filters have been shown to be effective in instruments with bandwidths of 2.2 nm to 6.5 nm.

Set your spectrophotometer in either absorbance or transmission at 440.0 nm. Using the empty filter holder (blank) zero the instrument. Insert each filter in turn and note the value. Set your instrument to each successive wavelength and repeat the procedure.

Compare the spectrophotometer absorbance values to the certified absorbance values. Taking the expanded uncertainty budget† of the certified references into consideration, if the absorbance values fall within the expected parameters† of your instrument then your instrument is working correctly. These readings can be plotted to check the instrument linearity.

For wavelength validation the procedure should be to scan the

Holmium wavelength reference filter over the usable range to ensure that all of the peaks can be resolved.

Adjust the slit width (SBW) and scan rate to produce a usable spectra. Check each peak to assure that the reading on your spectrophotometer is within the manufacturer's tolerances for the wavelength of your spectrophotometer.

Spectra should be checked regularly, at a minimum in the area of regular analysis. Periodic use of the Neutral Density and Holmium references will enable the user to build a data log of the instrument’s spectral accuracy for use with validation and troubleshooting should the correlation change over time

Set RM-1N3N5DHGThis set consists of 3 neutral density filters, a blank holder and a Holmium Glass filter.

Neutral Density filter's Approximate values are: %T Transmittance Absorbance 10% 1.00 A 30% 0.50 A 52% 0.25 A

The Holmium Glass filter is certified at 11 distinctive peaks ranging from 241 to 640. The seven certified Spectral Band Widths (SBW) measured are 0.10, 0.25, 0.50, 1.00, 1.50, 2.00, and 3.00 nm.


Neutral Density & Wavelength Filter set, NIST Traceable RM-1N3N5DHG $ 1650.00






Microplate Reader Visible Reference PlateDescription: 96 well microplate validation filters for wavelength and absorbance scales, NIST

Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Validation of the visible of photometric scale of 96 well microplate readers.

Certified Wavelengths: Wavelength: Neutral Density - 440.0, 465.0, 546.1, 590.0, 635.0 nm. Holmium - 360, 418, 445, 453, 460, 536, 637 nm, Absorbance scale: 440 nm to 635 nm

Physical Configuration: Glass Filters mounted in 96 well format anodized aluminum holder

Our 96 well microplate reader filter sets are based on and traceable to the NIST sets SRM 930e, SRM 1930 and NIST 2034. This microplate is used to test the photometric scale in both Transmission and Absorbance and wavelength accuracy in the visible wavelength range.

The absorbance filters are made from Schott NG-type glass, which absorbs a known percentage of the light passing through it. The thickness of the particular glass type used is calculated to produce filters of approximate transmittance/absorbance values, these approximate values are shown on the chart below. Each NIST traceable filter is individually tested and certified. The wavelength filter is made of Holmium glass and is effective across the visible range. The filters have been shown to be effective in all microplate readers on the market.

Description Part Number, NIST Traceable Price per set

96 Well Microplate Reader Validation Plate RMMR-1N3NN1N3HG $ 2,250.00

Configuration of Reference Plate:

Well Positions Filter Type Filter Values

C2 - H2 Air Blank C4 - H4 Neutral Density 30%T (0.5 Absorbance) C6 - H6 Neutral Density 10%T (1.0 Absorbance) C8 - H8 Neutral Density 3%T (1.5 Absorbance) C10 - H10 Neutral Density 1%T (2.0 Absorbance) C12 - H12 Holmium Peaks from 360 to 637 nm

Certified Wavelengths:

Neutral Density Absorbance Filters: 440.0 nm 465.0 nm 546.1 nm 590.0 nm 635.0 nm

Holmium Wavelength Filter (approximate wavelengths): 637 nm 536 nm 460 nm 453 nm 445 nm 418 nm 360 nm


Specifications:

Filters in reference plate:



Cuvette to 96 Well Microplate AdaptorDescription: A patented adaptor plate (Patent Number US8115922), which allows the use of standard cuvettes in the validation of a 96 well microplate reader.

Primary Usage: Performance validation of your microplate reader with Starna Reference sets

Usable Cuvettes: Standard rectangular cuvettes and filters.

Physical Appearance: Injection molded polymer tray with slots for 8 cuvettes

Compatible Readers: The adaptor is compatible with all 96 well plate readers


CuvettePlate, 96 well microplate adaptor SCP-96-8R $ 99.00

Hole alignment of the CuvettePlate conforms to the international standard and precise location of the wells in a 96 well microplate. Slots precisely align the cuvettes over the available holes through the bottom plate. A small ridge in the center of the CuvettePlate slightly tilts the cuvettes so that the bubble in a liquid sample does not interfere with the light beam of the microplate reader.

Validating your microplate reader with liquid filled cuvettes is easy with the CuvettePlate. Place your NIST traceable standards in the CuvettePlate and they will be aligned into the format for your reader and held so that the bubble in the cuvettes will not interfere with the analysis. You can use any of the Starna validation references as well as the NIST SRM-2034 Holmium.

Microplate readers can be validated using exactly the same NIST traceable filters either liquid or for instance Neutral Density Filters such as our Part Number RM-1N2N3N or NIST SRM-930), that you may already be using to calibrate other sectrophotometers in your laboratory.

Under these circumstances it is not necessary to purchase extra reference materials and allows the laboratory to cross validate and make comparisons between different types of spectrophotometer instruments.

Configuration of Reference Plate:

Well Positions Filter Type Filter Values

C2 - H2 Air Blank C4 - H4 Neutral Density 30%T (0.5 Absorbance) C6 - H6 Neutral Density 10%T (1.0 Absorbance) C8 - H8 Neutral Density 3%T (1.5 Absorbance) C10 - H10 Neutral Density 1%T (2.0 Absorbance) C12 - H12 Holmium Peaks from 360 to 637 nm




260/280 nm Validation Reference for DNA and RNADescription: Standard solution which gives a stable 260/280 nm ratio of 1.8 to 2.0, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration

Primary Usage: Control the quality of DNA/RNA purity analysis

Certified Wavelengths: 260 nm and 280 nm

Physical Configuration: Far UV quartz cell that has been permanently heat fusion sealed or vials with a crimp cap or screw cap

When running DNA or RNA purity analysis on your spectrophotometer, it is important to ensure that your instrument is working correctly. Our DNA/RNA validation standard in a permanently sealed quartz cell or vial (crimp or screw cap) contains a stable solution, which mimics the 260/280 nm ratio of DNA and RNA. The volume of each vial is 1.5 ml.

The RM-DNA (permanently sealed reference) is supplied with a certificate which lists the expected 260/280 nm ratio of the cell

and the confidence limit of the ratio. The validation analysis is performed by our ISO/IEC 17025 accredited laboratory and is traceable to NIST.

The vials with caps (both crimp cap and screw cap) reseal to maintain the integrity of the DNACON. This format allows for the removal of the DNACON by pipet or syringe. The vials are available in concentrations of 5x and 10x. The number of vials and the concentrations are listed below.


Permanently heat fusion sealed into a cuvette RM-DNA $ 990.00

5X concentration, 1 Crimp Cap Vial DNACON5X-CC1 $ 180.005X concentration, 2 Crimp Cap Vials DNACON5X-CC2 $ 340.005X concentration, 4 Crimp Cap Vials DNACON5X-CC4 $ 590.005X concentration, 1 Screw Cap Vial DNACON5X-SC1 $ 180.005X concentration, 2 Screw Cap Vials DNACON5X-SC2 $ 340.005X concentration, 4 Screw Cap Vials DNACON5X-SC4 $ 590.00

10X concentration, 1 Crimp Cap Vial DNACON10X-CC1 $ 180.0010X concentration, 2 Crimp Cap Vials DNACON10X-CC2 $ 340.0010X concentration, 4 Crimp Cap Vials DNACON10X-CC4 $ 590.0010X concentration, 1 Screw Cap Vial DNACON10X-SC1 $ 180.0010X concentration, 2 Screw Cap Vials DNACON10X-SC2 $ 340.0010X concentration, 4 Screw Cap Vials DNACON10X-SC4 $ 590.00

The importance of this reference is that it allows you to validate your work in real time. When measuring unknown samples you can assure that your instrument is working correctly by reading the validation standard both before, during and after each run of unknowns.

If you are using a spectrophotometer which automatically calculates the 260/280 ratio, then all that you need to do is place the DNA/RNA validation reference in your spectrophotometer and run the instrument's routine for the 260/280 ratio. If the ratio is valid when compared to the certificate then you can be assured that your instrument is calculating the ratio correctly. In a spectrophotometer without an automatic calculation feature, simply use the DNA/RNA validation reference as you would your other samples to validate your analytical procedure.

Generally, the 5x concentration is suitable for the Nanodrop instrument while the 10x concentration is suitable for micro drop systems such as Jenway/Bibby.

Additionally, at 260 nm, results are approximately as follows:

Light Path 5X Concentration 10X Concentration

0.2mm 0.1 A 0.2 A

0.5mm 0.25 A 0.5 A

1mm 0.5 A 1 A

10mm 5 A 10 A





260/280 nm Validation Reference for DNA and RNA

Typical Uncorrected Absorbance Values:

260 nm 0.765A280 nm 0.405A

Ratio: 1.89

RM-DNA DNACON 1.5 ml Vials

Crimp CapScrew Cap

0

0,2

0.4

0.6

0.8

1

1.2

220 240 260 280 300 320 340

Wavelengths (nm)

DNACON 260-280 Solution

Ab

sorb

ance

(A)


Quinine Sulfate Fluorescence ReferenceDescription & NIST Traceability: A solution of 1mg/L Quinine Sulfate in 0.105 mol/L Perchloric Acid. The reference material is permanently heat fusion sealed in quartz fluorometer cells, NIST Traceable complete with UKAS ISO/IEC 17025 accredited certificate of calibration.

Primary Usage: Calibration and evaluation of methods used on a fluorescence spectrophotometer.

Usable Range: 375 nm to 675 nm

Physical Configuration: Far UV quartz fluorescence cell that has been permanently heat fusion sealed. The reference is also available with a blank if required.

NIST SRM 936a is supplied as a crystalline material and is made into a solution as described in the Certificate of Analysis supplied with NIST SRM 936a. Starna then fills a high quality far UV quartz fluorometer cell with the SRM solution and permanently heat fusion seals the cell. The reference is designed for multiple use over time and for inter instrument comparisons. Quinine Sulfate references can also be supplied with a perchloric acid blank.

Where instrument linearity needs to be established, sets with different concentrations of Quinine Sulphate and a blank are available in the following concentrations, 0.25, 0.5, 0.75 & 1.0 mg/l.

The Starna reference set is supplied with a Certificate of Validation by our Calibration Laboratory which is accredited by UKAS under ISO 9001, ISO/IEC 17025, and ISO Guide 34. The certificate is valid for a period of two years.


Quinine Sulfate Reference (1 concentration 1.0 mg/l, without blank) RM-QS $ 1050.00Quinine Sulfate Reference (1 concentration 1.0 mg/l & blank) RM-QS00 $ 1400.00Quinine Sulfate Reference (2 concentrations, & blank) RM-2QS00 $ 2190.00Quinine Sulfate Reference (3 concentrations, & blank) RM-3QS00 $ 2980.00Quinine Sulfate Reference (4 concentrations, & blank) RM-4QS00 $ 3770.00

0.000

0.100

0.200

0.300

0.400

0.500

0.600

0.700

0.800

0.900

1.000

375.0 425.0 475.0 525.0 575.0 625.0 675.0

Ab

sorb

ance

(A)

Wavelengths (nm)

Quinine Sulphate

0.000

50.000

100.000

150.000

200.000

250.000

0.00 0.20 0.40 0.60 0.80 1.00 1.20

Rel

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its

(RIU

)

Quinine Sulfate (mg/l)

Quinine Sulfate Linearity

Corrected molecular emission spectrum

RM-QS

Linearity Regression Coefficient 0.999




Raman Reference, very high signal to noise ratioDescription: Ultra-high pure water, permanently heat fusion sealed in a high quality quartz fluorometer cell.

Primary Usage: Evaluation of sensitivity in the qualification of fluorescence spectrophotometers specifically the instrument Signal to Noise Ratio near the detection limits using the Raman band emission at specific wavelengths.

Usable Range: 250 to 600 nm

Physical Configuration: Far UV quartz fluorescence cell that has been permanently heat fusion sealed.

ISO/IEC Accreditation: Produced and manufactured under ISO/IEC 17025, ISO Guide 34 by UKAS, protocols

With years of development, Starna has perfected the technique of producing Ultra Pure water and permanently heat fusion sealing the water into a high quality Far UV quartz fluorometer cell.

The reference can be used multiple times on multiple instruments because the material does not deteriorate.

The Starna reference with a Certificate of Validation by our reference lab which is accredited by UKAS under ISO 9001, ISO/IEC 17025, and ISO Guide 34.


Ultra-pure Water Reference RM-H2O $ 1050.00

Molecular Emission SpectrumRaman Water SensitivityExcitation: 350 nm Emission Peak: 398 nm

Molecular Emission SpectrumRaman Water SensitivityExcitation: 500 nm Emission Peak: 600.4 nm



Raman Water Sensitivity

Wavelength (nm)

372

0

100

200

300

400

500

600

700

382 392 402 412 422 432 442 452 462

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its

(RIU

)

Wavelength (nm)

560 580 600 620 640 660

0

10

20

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40

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its

(RIU

)

Raman Water Sensitivity

Wavelength (nm)

372

0

100

200

300

400

500

600

700

382 392 402 412 422 432 442 452 462

Rel

ativ

e In

ten

sity

Un

its

(RIU

)

Wavelength (nm)

560 580 600 620 640 660

0

10

20

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Rel

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its

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)


Sets Recommended For Instrument QualificationTo make instrument Qualification easier, we have assembled reference materials into sets which allow you to meet specific verification requirements. It is more cost effective to purchase a group of references in a set instead of purchasing several references individually.

The following list of sets will allow you to meet the qualifications of the European Pharmacopoeia, the United States Pharmacopeia and sets that will satisfy the requirements of both the EP and USP.

Part Number: RM-0660HLKCTX NIST Traceable Price $ 3,090.00

This set is used to meet European Pharmacopoeia requirements and contains:

Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nmPotassium Dichromate 60 mg/L Photometric Accuracy 235 nm to 350 nmPotassium Dichromate 600 mg/L Photometric Accuracy 430 nmHolmium Oxide Wavelength Accuracy 240 nm to 650 nmStray Light blank Stray Light 200 nm to 400 nmPotassium Chloride, blank Stray Light 198 nmToluene in Hexane Resolution 267 nm to 268.7 nm

Part Number: RM-0660HLKCSITX NIST Traceable Price: $ 3,560.00

This set meets European Pharmacopoeia requirements as well as the United States Pharmacopeia requirements and contains:

Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 60 mg/L Photometric Accuracy 235 nm to 350 nm Potassium Dichromate 600mg/L Photometric Accuracy 430 nm Holmium Oxide Wavelength Accuracy 240 nm to 650 nm Stray Light blank Stray Light 200 nm to 400 nm Potassium Chloride Stray Light 198 nm Sodium Iodide Stray Light 220 nm Toluene in Hexane Resolution 267 nm to 268.7 nm

European Pharmacopoeia:

European Pharmacopoeia and USP:

Part Number: RM-0660HLKCSISNTX NIST Traceable Price: $ 4,030.00

This set meets European Pharmacopoeia requirements as well as the requirements of the United States Pharmacopeia.

Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nmPotassium Dichromate 60 mg/L Photometric Accuracy 235 nm to 350 nmPotassium Dichromate 600 mg/L Photometric Accuracy 430 nmHolmium Oxide Wavelength Accuracy 240 nm to 650 nmStray Light blank Stray Light 200 nm to 400 nmPotassium Chloride Stray Light 198 nmSodium Iodide Stray Light 220 nmSodium Nitrate Stray Light 340 nmToluene in Hexane Resolution 267 nm to 268.7 nm

Professional & Support Services Set for all Pharmacopoeias and USP:


Sets Recommended for Instrument Qualification

Part Number: RM-06HLKI NIST Traceable Price: $ 1,990.00

This set is primarily used in North America due to it's similarity to NIST SRMs and contains:

Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nmPotassium Dichromate 60 mg/L Photometric Accuracy 235 nm to 350 nmHolmium Perchloric Wavelength Accuracy 240 nm to 650 nmStray Light blank Stray Light 200 nm to 400 nmPotassium Iodide Stray Light 260 nm

Part Number: RM-06DLKI NIST Traceable Price: $ 2,220.00

This set is used where wavelength verification is required at higher visible wavelengths and contains:

Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nmPotassium Dichromate 60 mg/L Photometric Accuracy 235 nm to 350 nmDidymium Perchloric Wavelength Accuracy 290 nm to 870 nmStray Light blank Stray Light 200 nm to 400 nmPotassium Iodide Stray Light 260 nm

Part Number: RM-0204060810 NIST Traceable Price: $ 2130.00

With five concentrations of Potassium Dichromate, used to test the photometric scale and linearity between 0.095A and 1.5A.

Potassium Dichromate BlankPotassium Dichromate 20, 40, 60, 80, 100 mg/L Photometric Accuracy 235 nm to 350 nm

Part Number: RM-121416182224 NIST Traceable Price: $ 2,450.00

With six concentrations of Potassium Dichromate, used to test the photometric scale and linearity between 1.5A and 3.3A.

Potassium Dichromate BlankPotassium Dichromate 120, 140, 160, 180, 220 & 240 mg/L Photometric Accuracy 235 nm to 350 nm

Part Number: RM-040812162024 NIST Traceable Price: $ 2,450.00

With six concentrations of Potassium Dichromate used to test the photometric scale and linearity between 0.19A to 3.3A.

Potassium Dichromate BlankPotassium Dichromate 40, 80, 120, 160, 200 & 240 mg/L Photometric Accuracy 235 nm to 350 nm

Part Number: RM-020610HLKI NIST Traceable Price: $ 2,670.00

This set is used where absorbance linearity in the UV is required to be validated as well as the wavelength and stray light requirements of the USP. The set contains:

Potassium Dichromate blank Photometric Accuracy 235 nm to 350 nmPotassium Dichromate 20, 60, 100 mg/L Photometric Accuracy 235 nm to 350 nmHolmium Perchloric Wavelength Accuracy 240 nm to 650 nmPotassium Iodide, blank Stray Light 260 nm

United States Pharmacopeia set:

United States Pharmacopeia set with Didymium:

United States Pharmacopeia set with linearity:

UV Absorbance/Transmittance Scale & Linearity validation:


Product Summary and Price ListDescription: Part No. Price US$Photometric Scale Verification UVPotassium Dichromate20 mg/L RM-02 $ 830.0040 mg/L RM-04 $ 830.0060 mg/L RM-06 $ 830.0080 mg/L RM-08 $ 830.00100 mg/L RM-10 $ 830.00120 mg/L RM-12 $ 830.00140 mg/L RM-14 $ 830.00160 mg/L RM-16 $ 830.00180 mg/L RM-18 $ 830.00200 mg/L RM-20 $ 830.00220 mg/L RM-22 $ 830.00240 mg/L RM-24 $ 830.00600 mg/L RM-60 $ 830.00PDC 5 concentration set RM-0204060810 $ 2,130.00PDC 6 concentration set RM-121416182224 $ 2,450.00PDC 6 concentration set RM-040812162024 $ 2,450.00

Photometric Scale Verification UV.VISMetal on Quartz1% & Blank RM-Q1 $ 1290.003% & Blank RM-Q3 $ 1290.0010% & Blank RM-1Q $ 1290.0030% & Blank RM-3Q $ 1290.0050% & Blank RM-5Q $ 1290.0090% & Blank RM-9Q $ 1290.003%, 50% & Blank RM-Q35Q $ 1890.0010%, 30%, 90% & Blank RM-1Q3Q9Q $ 2850.00Starna Green25X conc & Blank RM-1SG $ 770.0050X conc & Blank RM-2SG $ 770.0075X conc & Blank RM-3SG $ 770.00100X conc & Blank RM-4SG $ 770.004 conc & Blank RM-1SG2SG3SG4SG $ 1,720.00

Far UV Photometric Scale VerificationNicotinic Acid6mg/L RM-1A $ 880.0012mg/L RM-2A $ 880.0018mg/L RM-3A $ 880.0024mg/L RM-4A $ 880.004 concentration set RM-1A2A3A4A $ 1,980.00

UV/VIS Wavelength Accuracy Holmium Glass filter RM-HG $ 350.00Didymium Glass filter RM-DG $ 480.00Holmium Oxide RM-HL $ 550.00Didymium Oxide RM-DL $ 770.00Samarium Oxide RM-SL $ 770.00

Far UV Wavelength Accuracy Rare Earth RM-RE $ 850.00

Stray LightSodium Iodide RM-SI $ 810.00Potassium Iodide RM-KI $ 810.00Lithium Carbonate RM-LC $ 810.00Potassium Chloride RM-KC $ 810.00Sodium Chloride RM-SC $ 810.00Sodium Nitrite RM-SN $ 810.00Acetone RM-AC $ 780.00

Instrument ResolutionBenzene Vapor RM-BZ $ 450.00

Description: Part No. Price US$SBW Bandwidth Calibration Toluene in Hexane RM-TX $ 860.00

Validation of 260/280 ratio DNACON Sealed Cell RM-DNA $ 990.00Crimp Cap Vial1 Vial 5X concentration DNACON5X-CC1 $ 180.002 Vials 5X concentration DNACON5X-CC2 $ 340.004 Vials 5X concentration DNACON5X-CC4 $ 590.001 Vial 10X concentration DNACON10X-CC1 $ 180.002 Vials 10X concentration DNACON10X-CC2 $ 340.004 Vials 10X concentration DNACON10X-CC4 $ 590.00Screw Cap Vial1 Vial 5X concentration DNACON5X-SC1 $ 180.002 Vials 5X concentration DNACON5X-SC2 $ 340.004 Vials 5X concentration DNACON5X-SC4 $ 590.001 Vial 10X concentration, DNACON10X-SC1 $ 180.002 Vial 10X concentration, DNACON10X-SC2 $ 340.004 Vial 10X concentration, DNACON10X-SC4 $ 590.00

Absorbance scale & Linearity VisibleNeutral Density Filter sets10, 20, 30%T RM-1N2N3N $ 1,190.001, 3, 50%T RM-N1N35N $ 1,190.000.1, 0.3, 92%T RM-D1D39N $ 1,190.00

Microplate Absorbance scale and Wavelength Accuracy VisibleMicroplate Reference RMMR-1N3NN1N3HG $ 2,250.00Microplate Cell Adaptor SCP-96-8R $ 99.00

Wavelength Accuracy NIRNIR transmittance reference RM-NIR $ 1,290.00NIR transmittance/reflectance reference RM-NIR/T $ 1,480.00

Photometric Scale Verification NIRMetal on Quartz1% & Blank RM-NQ1 $ 1290.003% & Blank RM-NQ3 $ 1290.0010% & Blank RM-1NQ $ 1290.0030% & Blank RM-3NQ $ 1290.0050% & Blank RM-5NQ $ 1290.0090% & Blank RM-9NQ $ 1290.003%, 50% & Blank RM-NQ35NQ $ 1890.0010%, 30%, 90% & Blank RM-1NQ3NQ9NQ $ 2850.00

Fluorescent ReferenceQuinine Sulfate 1 x 1.0 mg/l, RM-QS $ 1050.001 x1.0 mg/l & blank RM-QS00 $ 1400.002 concentrations, & blank RM-2QS00 $ 2190.003 concentrations, & blank RM-3QS00 $ 2980.00 4 concentrations, & blank RM-4QS00 $ 3770.00

Raman Reference, Very High Signal to Noise RatioUltra Pure Water RM-H20 $ 1050.00


Calibration Services & Certificates on USB StickCalibration Services

The Starna Calibration Laboratory offers a calibration and re-calibration service to ISO/IEC 17025, the highest level of accreditation currently available, second only to National Metrology Laboratories.

In addition to this calibration service being available for reference materials manufactured by Starna it is also available for many similar reference materials produced by third parties, including organisations such as NIST, Stranaska, as well as a number of other companies.

Starna Cells is also able to extend the service to cover a wide range of optical products within the UV/VIS/NIR range, We welcome enquiries for all types of similar product, even though in some instances they may not have been produced in the first instance as certified references.

Certification on USB Stick

Calibration Production Experience & Accreditation History

Starna has over forty-five years experience in the production of both Glass Filters and permanently heat fusion sealed liquid Reference materials.

Calibration accreditation through UKAS to ISO/IEC17025 was achieved in 2001. This was followed in 2006 by Starna also achieving ISO/IEC Guide 34 as a Certified Reference Material Producer. Calibration is carried out on any one of five fully qualified reference spectrophotometers.

The above accreditation, experience and accumulated data puts Starna in a unique position with regard to Certified Reference Materials ranging from the Deep UV to the NIR

The scope detailing specifically what the Starna accreditation covers, including uncertainty values, can be viewed at the following URL on the UKAS web site.www.ukas.org/AccredationOthers/schedules/4001%20RMP%20Single.pdfwww.ukas.org/calibration/schedules/Actual/0659Calibration%20Single.pdf

Guarantees

Expected Parameters and Expanded Uncertainty Budgets

Starna Reference Materials carry a lifetime Guarantee subject to: the references being re-calibrated by Starna at intervals not greater than two years; the references have not been subject to thermal, optical or physical abuse.

Starna will replace free of charge any references that, under normal environmental conditions, move outside their expanded uncertainty budget (k=3), within a two year period and cannot be corrected by cleaning or any other process.

The expanded uncertainty budget (k=2) is the tolerance that we could expect to measure in the assigned value on any one of our reference spectrophotometers during the 2 year period of certification.

When used to verify the performance of any given spectrophotometer this certificate tolerance must be added in a simple linear manner to the stated appropriate specification accuracy of the instrument on the test, see following example:

Wavelength AbsorbanceCertificate ±0.10 nm ±0.0049 AInstrument ±0.30 nm ±0.0050 ATotal ±0.40 nm ±0.0099 A

Certification Provided in USB Electronic Format

As a result of increased customer requests for certificates and associated data to be supplied in electronic format, especially from those customers working in controlled environments, in 2013 Starna changed certificates to an electronic format.

This advancement in presentation has helped the efficiency of Pharmaceutical companies, Instrument Manufacturers and other customers worldwide, another example of Starna taking the lead in reference material presentation. This environmentally friendly approach allows greater customer flexibility in the use and circulation of Calibration Certificates, allowing data to be input directly into other databases.

As part of the recalibration protocol certified references are measured as recieved, prior to any cleaning or other analysis.Many customers request the 'as received data' to be included when the sets are returned after recalibration, particularly where this is part of the customer ISO/IEC 17025 protocols.

This data is a good "health" indicator relating to the environment in which the references have been used and handled. Starna has often been able to offer advice in their use and handling, which has saved customers from unnecessary expense by replacing references, when it could otherwise have been avoided.

First Published 1994. Copyright January 2015 The copyright © of this brochure is held by Starna Cells, Inc

Product WarrantyAll Starna® Certified Reference Materials (CRMs) are warranted to meet the specifications defined in this brochure, when received by the customer. We offer a Lifetime Guarantee on all CRMs, providing that they are not physically, optically or thermally abused and that they have been re-certified at least every two years by Starna.

Any CRM that changes value to outside its expanded uncertainty budget (k=3), within a time frame of two years or less since its last certification by Starna, will be replaced free of charge. Warranty does not cover breakage. Any references to be returned under warranty require a Return of Merchandise Authorization (RMA) number. The RMA can be obtained by calling our customer service department.

RecertificationAll Starna NIST Traceable References can be re-certified and certificates issued for re-certified sets are identical in form to the certificates issued with new sets. Calculated uncertainty values are based on over fifteen years of Starna accredited data and over forty-five years production data.

Certificates are valid for a period of two years from date of issue and re-certification is recommended at either one or two year intervals depending on customer protocols. Please call for current pricing and return instructions. We also recertify references from NIST such as SRM 930, SRM 1930, SRM 2031 and SRM 2034 as well as other third party manufactured references.

Terms of SaleOur terms of sale are Mastercard, VISA, American Express or net 30 days to recognized accounts. If you are unsure of your account status, please call for verification of your account. The prices published in this brochure are in US$ and are current at the time of publication. They are subject to change without notice. The prices and our terms of shipment are calculated as FOB Atascadero, CA USA. We prepay shipping charges and add them to your charge card, invoice or UPS account.

World Wide Web:www.starnacells.com

Method of ShipmentWe usually ship via United Parcel Service and have all levels of service available including Red (overnight).

If required we can also ship via Federal Express. Orders for delivery outside of the USA are shipped via UPS or Fedex using either the customer's account or by prepaying shipping charges and adding them to the customer invoice.

Technical InformationWe have technical staff available to assist you with any information that you may need for the selection of a reference or its use. The best method of inquiry is via e-mail at: [email protected]. We are usually able to answer your inquiries within 24 hours via return e-mail.

Our hours of business are:8:30AM to 4:30PM, USA Pacific Time

DeliveryDelivery of new Certified Reference Materials is usually within two weeks from receipt of order. Delivery of re-calibrated sets of CRMs is approximately two weeks from receipt of the returned set at the Atascadero facility. All reference sets are calibrated immediately prior to dispatch from the accredited Starna Calibration Laboratory.

It is best practise to call for price and delivery verification either prior to or on placement of order. Every effort is made to keep delivery times to a minimum but, due to the certified nature of the product, we must do all that is necessary to assure that the reference materials are accurate, stable and properly documented as a priority.

Please call us at any time for a delivery estimate.

Mail orders: Phone Orders:Starna Cells, Inc (800) 228-4482 PO Box 1919 orAtascadero, CA 93423 (805) 466-8855

E-Mail Address: FAX Orders:[email protected] (805) 461-1575

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NIST Traceable UV/Vis/NIR Reference Sets

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