The How,Why & When of Non-surgical Sterilization PETER HOWE BVSC(HONS) MSCMED PHD MANZCVS

Introducing Non-surgical Sterilization Additional Considerations in Evaluations

HOW?

1. Vaccination to induce an immune response to endogenous hormones and other key proteins

GnRH - BSA Chemically conjugated GnRH - HSA Antigenic but poorly Immunogenic GnRH - KLH NEED ADJUVANTS - Freunds Complete and Incomplete GnRH LTB Chemically conjugated Active when administered parenterally and orally NEEDS ADJUVANTS - Freunds Complete and Incomplete Patented 1987 GnRH Vitamin B12 Chemically conjugated Orally active Patented 1987 ALL VERY EXPENSIVE TO PRODUCE

1. Vaccination to induce an immune response to endogenous hormones and other key proteins ( cont)

LTB- GnRH-GnRH-1/2(GnRH) Recombinant DNA product Very antigenic and immunogenic Highly irritant to tissues LPS Cheap to produce Patented 1987 Used in fish

2. Using Agonists and Antagonists to GnRH

3. Toxins that destroy specific key cell lines GnRH-Cis-platin GnRH- Pokeweed / (D-Lys6) GnRH -Pokeweed 4-Vinylcyclohexene Diepoxide ( Chemspay)

4. Intra-testicular injection of Seminiferous tubule, Sertoli and Leydig cell (mild) cytotoxins

Zinc Gluconate buffered with 0.2% Arginine

Calcium Chloride in alcohol ( Calchlor)

Lactic acid solutions

* Epididymal injections of Formol saline

4. Intra-testicular injection of Seminiferous tubule, Sertoli and Leydig cell cytotoxins ( Cont)

Neutersol

Esterisol

Zeuterin

5. Novel delivery systems for GnRH agonists/antagonists and other down-regulators of reproduction

Bacterial phages

Viruses - Adeno viruses

6. Other

Gene silencing - using micro RNAs - target Kisspeptin ( Kiss1 gene) and

Neurokinin B

Gene therapy

Other ( cont)

Why?

$

When?

Yuendumu Dog Health and Population Control Program 2 week program Treatment for ectoparasites and community education 37% reduction in dog population within 15 months Mange became extremely rare and general health and

body condition vastly improved Culture changed to seeing that having fewer dogs was

good ( Some surrendering and euthanasia occurred) Dogs With No Names - Canada Routinely use GnRH antagonists

GnRH Antagonists

* GnRH antagonists + Zeuterin

Zeuterin

It is not a matter of will non-surgical sterilization become commonly used , but when. Will we be ready when it does?

The How,Why & When of Non-surgical Sterilization Slide Number 2Slide Number 3Slide Number 4Slide Number 5Slide Number 6Slide Number 7Slide Number 8Slide Number 9Slide Number 10Slide Number 11Slide Number 12Slide Number 13Slide Number 14Slide Number 15Slide Number 16Slide Number 17Slide Number 18Slide Number 19Slide Number 20Slide Number 21Slide Number 22Slide Number 23Slide Number 24Slide Number 25Slide Number 26Slide Number 27Slide Number 28Slide Number 29Slide Number 30Slide Number 31Slide Number 32Slide Number 33Slide Number 34Slide Number 35Slide Number 36Slide Number 37Slide Number 38Slide Number 39Slide Number 40Slide Number 41Slide Number 42Slide Number 43Slide Number 44Slide Number 45Slide Number 46Slide Number 47Slide Number 48Slide Number 49Slide Number 50Slide Number 51Slide Number 52Slide Number 53Slide Number 54Slide Number 55Slide Number 56Slide Number 57Slide Number 58Slide Number 59Slide Number 60Slide Number 61Slide Number 62Slide Number 63