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Supplementary Information for Colloids and Surfaces B: Biointerfaces

Novel pH-sensitive interpenetrated network polyspheres of polyacrylamide-g-locust

bean gum and sodium alginate for intestinal targeting of ketoprofen: In vitro and in

vivo evaluation

Rashmi Boppanaa, Sushil Yadaorao Raut

b, G. Krishna Mohan

c, Biswanath Sa

d

Srinivas Mutalikb, Kakarla Raghava Reddy

e, Kusal K. Das

f, Mallanagouda S. Biradar

g

Raghavendra V. Kulkarnia,

*

aDepartment of Pharmaceutics & Pharmaceutical Technology, BLDEA’s SSM College of

Pharmacy & Research Centre, BLDE University Campus, Vijayapur 586 103, India.

bDepartment of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy

of Higher Education, Manipal, 576 104, India

cCentre for Pharmaceutical Sciences, Jawaharlal Nehru Technological University, Kukatpally,

Hyderabad 500 585, India

dDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata 700 032, India

eSchool of Chemical and Biomolecular Engineering, The University of Sydney,

Sydney, NSW 2006, Australia.

fDepartment of Physiology, Shri. B. M. Patil Medical College, Hospital and Research Centre,

BLDE (Deemed to be University), Vijayapur 586 103, India.

gDepartment of Medicine, Shri B. M. Patil Medical College, Hospital and Research Centre,

BLDE (Deemed to be University), Vijayapur 586 103, India.

* Corresponding author. E-mail address: pharma_75raghu@yahoo.com (R.V. Kulkarni)

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Figure captions

Fig. S1. Schematic representation for microwave assisted synthesis of pH-sensitive PAAm-g-

LBG copolymer.

Fig. S2. FTIR spectra of LBG (A), PAAm-g-LBG (B) and hydrolyzed PAAm-g-LBG (C).

Fig. S3. 1H NMR spectra of AAm (A), LBG (B) and PAAm-g-LBG (C).

Fig. S4. TGA thermograms of SA (A), PAAm-g-LBG (B) and drug free L9 IPN polyspheres (C).

Fig. S5. DSC thermograms of pristine ketoprofen (A), drug free L9 IPN polyspheres (B) and

drug loaded L9 IPN polyspheres (C).

Fig. S6. XRD diffractograms of pristine ketoprofen, drug free L9 IPN polyspheres and drug

loaded L9 IPN polyspheres.

Fig. S7. Plasma concentration of pristine ketoprofen (PD) and L9 IPN polyspheres in rats after

oral administration. Results are expressed as mean SD; n = 6.

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Fig. S1. Schematic representation for microwave assisted synthesis of pH-sensitive PAAm-g-

LBG copolymer.

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Fig. S2. FTIR spectra of LBG (A), PAAm-g-LBG (B) and hydrolyzed PAAm-g-LBG (C).

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Fig. S3. 1H NMR spectra of AAm (A), LBG (B) and PAAm-g-LBG (C).

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Table S1. Elemental analysis results

Codes % Nitrogen % Carbon % Hydrogen

LBG 0.44 38.55 6.72

PAAm-g-LBG 12.29 37.63 6.24

H-PAAm-g-LBG 3.58 31.71 6.07

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(Color for web version)

Fig. S4. TGA thermograms of SA (A), PAAm-g-LBG (B) and drug free L9 IPN polyspheres (C).

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(B/W for print version)

Fig. S4. TGA thermograms of SA (A), PAAm-g-LBG (B) and drug free L9 IPN polyspheres (C).

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Fig. S5. DSC thermograms of pristine ketoprofen (A), drug free L9 IPN polyspheres (B) and

drug loaded L9 IPN polyspheres (C).

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Fig. S6. XRD diffractograms of pristine ketoprofen, drug free L9 IPN polyspheres and drug

loaded L9 IPN polyspheres.

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(Color for web version)

Fig. S7. Plasma concentration of pristine ketoprofen (PD) and L9 IPN polyspheres in rats after

oral administration. Results are expressed as mean SD; n = 6.

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(B/W for print version)

Fig. S7. Plasma concentration of pristine ketoprofen (PD) and L9 IPN polyspheres in rats after

oral administration. Results are expressed as mean SD; n = 6.