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www.mn-net.comMACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · GermanyFrance:MACHEREY-NAGEL EURLTel.: +33 388 68 22 68Fax: +33 388 51 76 88E-mail: [email protected]
Switzerland:MACHEREY-NAGEL AGTel.: +41 62 388 55 00Fax: +41 62 388 55 05E-mail: [email protected]
Germanyand international:Tel.: +49 24 21 969-0Fax: +49 24 21 969-199E-mail: [email protected]
MACHEREY-NAGEL
EN ISO 9001: 2008CERTIFIED
USA:MACHEREY-NAGEL Inc.Tel.: +1 484 821 0984Fax: +1 484 821 1272E-mail: [email protected]
Highest efficiency in HPLC by core-shell technology
NUCLE SHELL®
. . . we Meet your Needs
Includin
g
NEW
Phenyl−
Hexyl
phase
www.mn-net.com2
Ultrafast separations beyond high pressure driven UHPLCNUCLEOSHELL® 2.7 µm
0.5 μm
0.5 μm
shell of porous silica
shell of porous silica
1.7 μm solid core of silicon dioxide
Core-shell technology Solid core of silicon dioxide, homogeneous
shell of porous silica Highestefficiencycomparedtototally
porous silica particles Particlesize2.7µm(core1.7µm), poresize90Å,specificsurface130m2/g; lower back pressure enables use on conventionalLCsystems Pressurestabilityupto600 bar
NUCLEOSHELL® modificationsTheprogramofsurfacemodificationsbasedon NUCLEOSHELL® silica now comprises the following phases:
NUCLEOSHELL®RP18 NUCLEOSHELL®Phenyl-HexylNEW! NUCLEOSHELL® PFP NUCLEOSHELL® HILIC
Several approaches have been made to achieve fast separations without losing chromato-graphic performance. HPLC columns packed with particles < 2 µm show very high efficien-cies (plates/meter) and allow the use of small-er column sizes with the positive side effect of significant solvent saving. However they gen-erate a high back pressure of the mobile phase during column runs which requires specifically designed equipment.
Core-shell particle technology from MACHEREY-NAGEL is an alternate route to gain highest column efficiency and resolution at almost the same short run time but with much lower back pressure.
NUCLEOSHELL® silica particles consist of a non-poroussolidcoreof1.7µmdiameterandaporousouter shell of0.5µm thickness.Accordingly thetotaldiameteroftheparticleis2.7µm.Withconventionalfullyporousparticlesthemasstransfer between stationary and mobile phase
usuallyresultsinpeakbroadeningathigherflowrates(C-terminvanDeemterequation).Theshortdiffusionpaths inthecore-shellparticlesreducethedwelltimeoftheanalytemoleculesinthesta-tionaryphase,sothatevenathighflowvelocitiesof the mobile phase, optimal separation results can be obtained.The van Deemter plots on page 3 demonstratehowefficiencyisaffectedbyflowrate.Incompari-sonwith fullyporoussilicas, core-shellparticlesfromvariousmanufacturersmaintaintheefficien-cyoptimum(max.plates/m)overalongrangeofincreasinglinearmobilephasevelocity.
Theoretical column efficiency (optimal conditions)Silica phase dp
[µm]L [m]
HETP [µm]
Efficiency [plates/m]
L [mm]
N Rs Analysistime
NUCLEOSHELL® 2.7 1 4 250 000 100 25 000 112 % 40 %NUCLEODUR® 1.8 1 4.5 222 222 100 22 000 105% 40%
3 1 7.5 133333 150 20 000 100% 60%5 1 12.5 80000 250 20 000 100% 100%
Benefits of core-shell technology Core-shell particles vs. totally porous silica gel
Short diffusion paths Fastmasstransfer(termCofVanDeemterequation) HighflowvelocitywithoutpeakbroadeningforfastLC
Narrow particle size distribution (d90/d10 ~ 1.1) Stable packing
High heat transfer Minimizedinfluenceoffrictionalheat EfficiencyofNUCLEOSHELL® ~ 250 000 m–1 (HETP~4µm)
3www.mn-net.com
NUCLEOSHELL®Core-shell silicaDemands on HPLC separations are constantly increasing with respect to separation efficiency, detection limits, and the time requirements for each analysis. Core-shell technology sets new standards for analyses in research and quality control.
Rs =N4
ki’+1ki’
1αα–
Rs = Resolution a = Selectivityki’ = Retention N = Theoretical plates N ∝ 1/dPdP = Particle size
Resolution RS as function of particle sizeColumns: 50 x 4 mm each
NUCLEOSHELL® RP 18, 2.7 μm NUCLEODUR® C18 Gravity, 3 μm NUCLEODUR® C18 Gravity, 1.8 μm
Eluent: acetonitrile – water (60:40, v/v) Flow rate: 1 mL/minTemperature: 25 °CDetection: UV, 254 nmPeaks: 1. Naphthalene2. Ethylbenzene
Better resolution at lower back pressureand shorterretention time
1
2
22
11
0.0 0.5 1.0 1.5 2.0 2.5 min
RS = 2.47104 bar
RS = 2.17190 bar
RS = 1.7188 bar
MN Appl. No. 125270
Electron microscopic image of NUCLEOSHELL® particlesUtilizingaproprietaryprocessofsyn-thesis, NUCLEOSHELL® particles ex-hibit a distinct narrow particle size distribution(d90/d10~1.1).Columnspacked with NUCLEOSHELL® core shell particles feature exceptional separa-tionefficiencieswith theoreticalplatenumbers easily comparable to totallyporous sub 2 micron particles.
Van Deemter plots
0
2
4
6
8
10
12
14
16
18
20
0 2 4 6 8 10
Term A:Term B:Term C:
Eddy diffusion Longitudinal diffusion Mass transfer
Columns:Eluent:Temperature:Sample:
50 x 4.6 mmCH3CN – H2O (70:30, v/v)
Acenaphthene25 °C
Linear velocity u [mm/s]
Plat
e he
ight
h [μ
m]
h = A + Bu--- + C · u
NUCLEOSHELL® RP 18
NUCLEODUR® C18 Gravity, 1.8 μm
Kinetex® C18
Ascentis® Express C18
Poroshell 120 EC-C18NUCLEODUR® C18 Gravity, 3 μm
MN Appl. No. 125500
Pressure drop
0
100
200
300
400
500
600
0 2 4 6 8 10 12
Kinetex® C18
Ascentis® Express C18
Poroshell 120 EC-C18
NUCLEODUR® C18 Gravity, 3 μm
dp2
Φ · LC · η · uΔp =
NUCLEOSHELL® RP 18
Columns:Eluent:Temperature:
50 x 4.6 mmCH3CN – H2O (70:30, v/v)25 °C
Pres
sure
[bar
]
Linear velocity [mm/s]
= pressure drop= flow resistance (nondimensional)= column length= viscosity= linear velocity = particle diameter
ΔpΦLCηu dp
NUCLEODUR® C18 Gravity, 1.8 μm
MN Appl. No. 125510
In direct comparison with the “con-ventional” sub 2 micron phas-es, NUCLEOSHELL® columns onlygenerate about 60% of the backpressure and can be operated with the majority of conventionalHPLC systems. In order to devel-op the maximum performance of NUCLEOSHELL® columns, we rec-ommend reducing extra column voids by using suitable capillaries(<0.15mm inner diameter) andspecially adapted detector cells.Moreover detector settings should be optimized by increasing themeasuring rate or by decrease ofthe time constant.
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Features of core-shell silica particlesStability under acidic and basic
conditionsColumn: 50 x 4.6 mm NUCLEOSHELL® RP 18, 2.7 μm
50 x 4.6 mm Kinetex® 2.6 μm C18 Eluent: acetonitrile – 1 % TFA in H2O, pH 1 (50:50, v/v)Flow rate: 1.3 mL/min; temperature 80 °CDetection: UV, 254 nmSample: anthracene
0
20
40
60
80
100
0 5000 10000 15000 20000 25000 30000
pH 1
NUCLEOSHELL®
Kinetex®
Column volumes
% in
ital r
eten
tion
(ant
hrac
ene)
Columns: 50 x 4.6 mm NUCLEOSHELL® RP 18, 2.7 μm 50 x 4.6 mm Ascentis® Express C18, 2.7 μm 50 x 4.6 mm Poroshell 120 EC-C18 50 x 4.6 mm Kinetex® 2.6 μm C18
Eluent: 20 mmol/L Na borate – 10 mmol/L NaOH – methanol, pH 10 (21:49:30, v/v))
Flow rate: 1.5 mL/min; temperature 40 °CDetection: UV, 220 nmSample: toluidine
0
2000
4000
6000
8000
10000
12000
0 5000 10000 15000 20000
pH 10
Ascentis® ExpressNUCLEOSHELL®
Poroshell 120Kinetex®
Plat
es (t
olui
dine
)
Column volumes
MN Appl. Nos. 125520 / 125530
TheabovefigureshowsacolumnstabilitytestofNUCLEOSHELL®RP18atmobilephaselevelspH1andpH10comparedwiththreecompetingphases.
Acriterionforthelong-termstabilityofthecol-umn at pH extremes is the percentage decrease ofinitialretentionandinitialplates,respectively.The column can also be operated at elevated tem-peratures without loss in retention behavior, ef-ficiencyorpeaksymmetry.
Temperature stabilityStability test:Column: 50 x 2 mm NUCLEOSHELL® RP 18, 2.7 μmEluent: A) 10 mmol/L ammonium formate – methanol
(9:1, v/v) + 120 μL formic acid, ~ pH 4 B) 10 mmol/L ammonium formate – methanol (1:9, v/v) + 120 μL formic acid, ~ pH 4 0–100 % B in 7 min
Flow rate: 0.5 mL/minTemperature: 100 °CDetection: UV, 220 nmPeaks:1. Phenol2. Naphthalene
0
1
2
2 4 6 8 min
38 h34 h30 h26 h22 h
MN Appl. No. 125400Efficiency test:Eluent: acetonitrile – water (60:40, v/v)Flow rate: 0.33 mL/minTemperature: 25 °CDetection: UV, 254 nmSample: anthracene
HETP[µm] AsymmetryStart(t=0) 5.2 0.98End(t=40h) 5.2 1.01
Batch-to-batch reproducibilityColumn: 50 x 4 mm NUCLEOSHELL® RP 18, 2.7 μmEluent: methanol – 25 mmol/L KH2PO4 pH 7
(70:30, v/v)Flow rate: 1 mL/minTemperature: 40 °CDetection: UV, 254 nmPeaks:1. Uracil2. Toluene3. Ethylbenzene4. Acenapthene5. Amitriptyline6. o-Terphenyl7. Triphenylene
0 2 4 6 8 10
3
4
56
721
min
Batch 1
Batch 2
Batch 3
MN Appl. No. 125410
Uniformly shapedNUCLEOSHELL® particles com-bined with optimized bonding technology safe-guardtightlypackedcolumnsfor100%reproduc-ible results.
5www.mn-net.com
NUCLEOSHELL®
Peak capacityColumns: 100 x 4.6 mm each
NUCLEOSHELL® RP 18, 2.7 μm NUCLEODUR® C18 Gravity, 1.8 μm NUCLEODUR® C18 Gravity, 3 μm NUCLEODUR® C18 Gravity, 5 μm
Eluent: A) acetonitrile, B) water, 40–100 % A in 4 minFlow rate: 1.5 mL/minTemperature: 25 °CDetection: UV, 230 nmPeaks:1. Acetophenone2. Benzoin3. Propiophenone
4. Butyrophenone5. Benzophenone6. Valerophenone
0 1 2 3 4 min
3 4
56
2
1
MN Appl. No. 125540
Max. pressure [bar]
Resolution (4,5)
NUCLEOSHELL®, 2.7 µm 255 5.45NUCLEODUR®,1.8µm 450 4.14NUCLEODUR®,3µm 214 2.97NUCLEODUR®,5µm 142 2.30
Peak capacity
100125
163
216
0
50
100
150
200
250
nc:tg:W:
NUCLEODUR®5 μm 3 μm
NUCLEOSHELL®
Wtgnc = 1 + ( )
peak capacitygradient timepeak width (at baseline)
1.8 μm 2.7 μm
n c (n
orm
aliz
ed)
Thepeakcapacityisameasureofthenumberofsample analytes that can be separated on HPLCcolumns per time unit. Narrow peaks increase thepeakcapacityandefficiencyofanalyticalcol-umns. The example shows, that in comparison with totally porous NUCLEODUR® silica (1.8µm)NUCLEOSHELL®provides33%higherpeakcapac-ity.
Loading capacityNUCLEOSHELL® columns allow reliable quantifi-cationinawideanalyticaldetectionrange.Reten-tion time and peak width at 50% height remainconstant with increasing column load although core-shell particles are suspected of showing aslightly lower loadingcapacity compared to fullyporous silica materials.
Loading capacityColumn: 50 x 3 mm NUCLEOSHELL® RP 18, 2.7 μmEluent: acetonitrile – 25 mmol/L KH2PO4, pH 3
(70:30, v/v)Flow rate: 0.66 mL/minTemperature: 30 °CDetection: UV, 285 nmPeaks:1. Valerophenone
9 μg
0 1 2 3 min
3 μg
0.9 μg0.3 μg
0
200
400
600
800
0 2 4 6 8 10
R2 = 0.9991
Area
Load on column [μg]
Normalized column parameters
0 2 4 6 8 10
Peak width(at 50 % peak height)
Retention time
Efficiency(at 10 % peak height)
Load on column [μg]
0
0.2
0.4
0.6
0.8
1
1.2
www.mn-net.com6
NUCLEOSHELL® RP 18 Key features:
·Basedoncore-shellparticletechnol-ogyforfastandefficientHPLC
· Suitable for LC/MS and HPLC at pH extremes(pH1–11)
· Superior base deactivation, ideal for method development
Technical characteristics: Octadecylmodification,multi-
endcapped; pore size 90 Å, particle size2.7μm,carboncontent7.5%
Recommended application: Overallsophisticatedanalyticalseparations, e.g., analgesics, anti-inflammatorydrugs,antidepressants; herbicides; phytopharmaceuticals;immunosuppressants
USP L1
NUCLEOSHELL® RP 18NUCLEOSHELL®RP18isbasedoncore-shellpar-ticle technology silica. A unique derivatizationprocess generates a homogeneous surface with ahighdensityofbondedsilanes(carboncontent~7.5%).Thefollowingthoroughendcappingsup-pressesanyunwantedpolarinteractionsbetweenthe silica surface and the sample, which makes NUCLEOSHELL®RP18particularlysuitablefortheseparationofbasicandotherionizableanalytes.Theextremelyreducedsilanolactivityofthephasecanbedemonstratedbyapplyingbasicanalytes,suchas tricyclic antidepressants.The chromato-gram on page 7 shows a sharp elution profile(superior resolution!)of thesehighlypolar com-pounds with an excellent asymmetry value foramitriptylineof1.12.
Tanaka plot of NUCLEOSHELL® RP 18 Thediagrambelowunderlinesthedistincthydro-phobiccharacteristicsandthelowsilanolactivityofthestationaryphase.
0.5
1
5
10
0.5
1
1
2
0.1
0.2
1
2
hydrogen bonding capacity
capacity
ion exchange capacity pH 7.6
hydrophobicity
ion exchange capacity pH 2.7
stericselectivity
Parameters of the Tanaka diagramCapacity=k’(pentylbenzene)Hydrophobicity=α(pentylbenzene,butylbenzene)Stericselectivity=α(triphenylene,o-terphenyl)Hydrogenbondingcapacity(silanolcapacity)=α(caffeine,phenol)Ionexchangecapacityat2differentpHvalues(2.7and7.6)=α(benzylamine,phenol)
The separation of 13 β-lactam antibiotics illus-trateshowtimeofanalysiscanbeshortenedtoafractionalpartbyusingcore-shellparticleswith-out loss of resolution at moderate back pressure.
13 β-lactam antibiotics in less than 3 minColumns: 50 x 4 mm NUCLEOSHELL® RP 18, 2.7 μm
150 x 4 mm NUCLEODUR® C18 Gravity, 5 μmEluent: A) acetonitrile; B) 20 mmol/L KH2PO4 pH 3.5
10 % A (0.5 min) → 50 % A in 1.5 min (0.5 min 50 % A) 10 % A (3 min) → 50 % A in 9 min (3 min 50 % A)
Flow rate: 2 mL/min, 1 mL/minPressure: 270 bar, 110 barTemperature: 25 °C Detection: UV, 220 nmPeaks:1. Amoxicillin2. Ampicillin3. Cephalexin4. Cefotaxime5. Cefoxitin
6. Cefamandole7. Cephalothin8. Piperacillin9. Penicillin V10. Oxacillin
11. Cloxacillin12. Nafcillin13. Dicloxacillin
0.0 0.4 0.8 1.2 1.6 2.0 min
2.5 min270 bar
0 2 4 6 8 10 12 min
1
23
46
5
7 8 9
1011
1213
1
23 4
6
5
7 89
10 11
12
13
MN Appl. No. 124940
7www.mn-net.com
NUCLEOSHELL®
Tricyclic antidepressants · comparison of selectivity and resolutionColumns: 50 x 4.6 mm each
NUCLEOSHELL® RP 18, 2.7 μm Ascentis® Express C18 Kinetex® 2.6 μm C18 Poroshell 120 EC-C18
Eluent: methanol – acetonitrile – 25 mmol/L KH2PO4 pH 7 (22.5:22.5:55, v/v/v)
Flow rate: 2 mL/min Pressure: 224 bar, 239 bar, 248 bar, 212 barTemperature: 40 °C Detection: UV, 220 nm
Asymmetry (amitriptyline)
Resolution (8, 9)
NUCLEOSHELL® 1.12 3.35Ascentis® Express 2.07 1.91Kinetex® 1.33 n.a.Poroshell 1.05 1.95
1
2
3
7
89
6
5
4
0 2 4 6 8 10 12 14 16 18 min
1
2
3
4
0 1 32 min
NUCLEOSHELL®
Ascentis®
Kinetex®
Poroshell
Peaks:1. Protriptyline2. Desipramine3. Maprotiline4. Nortriptyline5. Doxepin6. Imipramine7. Amitriptyline8. Clomipramine9. Trimipramine
MN Appl. No. 124960
NUCLEOSHELL® RP 18 combines innovative silica technology and excellent surface de-activation, that outperforms conventional C18 silicas in terms of efficiency, resolution and speed. Due to the applied core-shell particle design the back pressure at elevated flow rates remains at a moderate level and per-mits the use of existing HPLC equipment in many cases. NUCLEOSHELL® RP 18 with ex-tended pH stability, low bleed characteristics in LC/MS applications and overall robustness is an ideal tool for method development and routine analysis in modern HPLC.
www.mn-net.com8
Key features: ·Basedoncore-shellparticletechnol-
ogyforfastandefficientHPLC ·Hydrophobicphasewithalternative
selectivityincomparisontoclassicalC18modifications
· Separation principle based on 2 re-tention mechanisms: π-π interactions andhydrophobicinteractions
Technical characteristics: Phenyl-Hexylmodification,multi-
endcapped; pore size 90 Å, particle size2.7μm,carboncontent4.5%; pHstability1–10;suitableforLC/MS
Recommended application: Aromaticandunsaturatedcompounds,polar compounds like pharmaceuticals, antibiotics
USP L11
Phenyl-Hexylmodified phases offer an excellentseparation efficiency especially for aromatic andunsaturatedcompoundswithelectron-withdraw-inggroups.Thecombinationofhydrophobicandπ-πinteractionsresultsinanalternativeandin-terestingselectivityprofilecomparedtheC18 or C8
Bleeding characteristics of NUCLEOSHELL® Phenyl-HexylColumns: 50 x 2 mm each
NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm Kinetex® Phenyl-Hexyl
Eluent: A) acetonitrile, B) water; 5–95 % A in 25 min
Flow rate: 0.2 mL/minTemperature: 25 °CDetection: MS
MN Appl. No. 126400 4 8 12 16 20 min0
1.0e8
5.0e7
cps
Temperature stability of NUCLEOSHELL® Phenyl-Hexyl
Column: 50 x 2 mm NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm
Eluent: acetonitrile – 10 mmol/L ammonium for-mate pH 4 (50:50, v/v)
Flow rate: 0.33 mL/minTemperature: 100 °CDetection: UV, 254 nmInjection: 0.2 μLPeaks:1. Phenol2. Naphthalene3. Anthracene
MN Appl. No. 126430
modifications. NUCLEOSHELL® Phenyl-Hexyl isbasedonanuniquesurfacebondingchemistry-therefore it is suitable for LC/MS due to low bleed-ing characteristics and offers high temperaturestabilityandpHstabilityfrom1to10.
Stability of NUCLEOSHELL® Phenyl-Hexyl at pH 10
Column: 50 x 4 mm NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm
Eluent: acetonitrile – 50 mmol/L TEA pH 10 (60:40, v/v); pH of the mixture 10.4
Flow rate: 1 mL/minTemperature: 40 °CDetection: UV, 254 nmInjection: 1 μLPeaks:1. Phenol2. Naphthalene3. Anthracene
Relative plate numbers
60
80
100%
0 10 20 30 40 50 h
MN Appl. No. 126420
NUCLEOSHELL® Phenyl-Hexyl is a robust phase with an alternative RP selectivity for aromatic and unsaturated analytes com-pared to classical C18 / C8 phases - it is an additional and useful tool for all chromatog-raphers.
0 1 2 min
0 h45 h
12
3
0 1 2 min
12
3
0 h60 h
NUCLEOSHELL® Phenyl-Hexyl
9www.mn-net.com
NUCLEOSHELL®
Comparison of Phenyl-Hexyl phases for the separation of sulfonamidesColumns: 150 x 3 mm each
NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm NUCLEODUR® Phenyl-Hexyl, 1.8 μm NUCLEODUR® Phenyl-Hexyl, 3 μm NUCLEODUR® Phenyl-Hexyl, 5 μm
Eluent: A) methanol, B) 0.1 % formic acid in water, 20–80 % A in 10 min
Flow rate: 0.56 mL/minTemperature: 40 °CDetection: UV, 254 nmInjection: 0.5 μLPeaks:1. Sulfadiazine2. Sulfachloropyridazine3. Sulfapyridine4. Sulfamerazine5. Sulfadimidine6. Sulfathiazole7. Sulfadimethoxine
On NUCLEOSHELL® Phenyl-Hexyl the resolution of the last two peaks is higher than on the fully porous 1.8 μm NUCLEODUR® Phenyl-Hexyl.
MN Appl. No. 125860 0 2 4 6 8 min
1
23
4
5
6
7
Rs26.46
Rs16.03
Rs16.83
Rs13.54
The separation of sulfonamides proves the scalability from fully porous NUCLEODUR® to NUCLEOSHELL® Phenyl-Hexyl. Hereby the core-shell silica exhibits under same conditions identi-calselectivity,narrowerpeaksandslightlyshorterretention. Thus, method transferability betweenNUCLEODUR® and NUCLEOSHELL® is guaranteed,
eitherforspeedingupyourmethodsorscalingupforpreparativerequirements.Thepyridine-phenoltest shows that NUCLEOSHELL®Phenyl-Hexylpro-videsasymmetricalpeakforpyridineandhigherresolutionincomparisontoothercore-shellbasedPhenyl-Hexylphases,whichunderlinestheexcel-lent base deactivation.
Pyridine – phenol testColumns: 50 x 2 mm each
NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm Kinetex® Phenyl-Hexyl Ascentis® Express Phenyl-Hexyl
Eluent: acetonitrile – water (70:30, v/v)Flow rate: 0.3 mL/minTemperature: 40 °CDetection: UV, 254 nm
1
2
min0.0 0.5 1.0
Peaks:Injection volume 0.2 μL1. Pyridine2. Phenol
MN Appl. No. 126410
www.mn-net.com10
Key features: ·Basedoncore-shellparticletechnol-
ogyforfastandefficientHPLC ·Hydrophobicphasewithalternative
selectivityincomparisontoclassicalC18modifications
· Separation principle based on 4 re-tention mechanisms: -polarinteractions(Hbonds) -dipole-dipoleinteractions -π-π interactions -hydrophobicinteractions
Technical characteristics: Pentafluorophenylpropylmodification,multi-endcapped;poresize90Å,par-ticlesize2.7μm,carboncontent~3%;pHstability1–9;suitableforLC/MS
Recommended application: Aromaticandunsaturatedcompounds,phenols, halogenated compounds, isomers, polar compounds like pharmaceuticals, antibiotics; high retention of basic compounds
USP L43
Orthogonality in selectivityFluorinatedstationaryphasesinHPLChavegainedincreasinginterestoverthelastyears.Mostcom-monrepresentativeoffluorinatedsilicaphasesisthe pentafluorophenyl modification (PFP or F5).Especially theorthogonalselectivitycomparedtotraditionalalkylphaseswidensthescopeinana-lytical HPLC. Thus NUCLEOSHELL® PFP offers anexcellent selectivity especially for highly polaranalytes, aromatic and unsaturated compounds,phenolsorhalogenatedhydrocarbons.
Halogen substitutes in molecules result often in anincreaseoftheirpolarityaccompaniedbyade-crease of typical retention characteristics in RP-HPLC.WhileatypicalC18phasejustprovideshydrophobicinteractionsbetweenstationaryphaseandanalyteNUCLEOSHELL®PFPoffersfourdifferentretentionmechanisms:polarinteractions(Hbonds),dipole-dipole interactions,π-π interactionsandhydro-phobicinteractions.Especiallythepronouncedionexchange capacity and distinct steric selectivityaretypicalforthecharacteroffluorinatedphases.
Tanaka plot of NUCLEOSHELL® PFP
0.5
1
5
10
0.5
1
1
2
0.1
0.2
1
2
hydrogen bonding capacity
capacity
ion exchange capacity pH 7.6
hydrophobicity
ion exchange capacity pH 2.7
stericselectivity
NUCLEOSHELL® PFP combines the benefits of core-shell technology, high stability and orthogonal selectivity. So it is a useful complementary tool for highly efficient separations especially of iso-mers, halogenated, aromatic and / or polar compounds.
β-Blockers · orthogonal selectivity of NUCLEOSHELL® PFP
Columns: 100 x 4.6 mm each NUCLEOSHELL® RP 18, 2.7 μm NUCLEOSHELL® PFP, 2.7 μm
Eluents: A) acetonitrile + 0.1 % formic acid; B) 0.1 % formic acid; 10–35 % A in 2.5 min, 35-50 % A in 2 min
Flow rate: 1.7 mL/min Temperature: 25 °C Detection: UV, 280 nmPeaks:1. Atenolol2. Pindolol3. Metroprolol4. Labetalol5. Alprenolol6. Propranolol
0 1 2 3 4 min
1
2
3
4
6
5
1
2
3
4 5
6
MN Appl. No. 125610
NUCLEOSHELL® PFP
11www.mn-net.com
NUCLEOSHELL®
Stability of NUCLEOSHELL® PFP at pH 1Columns: 100 x 4,6 mm NUCLEOSHELL® PFP, 2.7 μm
100 x 4.6 mm Kinetex® 2.6 μm PFPEluent: acetonitrile – 0.5% TFA pH 1 (50:50, v/v)Flow rate: 1.3 mL/min Temperature: 60 °C Detection: UV, 254 nmSample:Ethylbenzene
0
20
40
60
80
100
120
0 2000 4000 6000
NUCLEOSHELL® PFP
% initial retention
% initial plates
0
20
40
60
80
100
120
0 2000 4000 6000
Kinetex® PFP
Column volumes
% initial retention
% initial plates
MN Appl. No. 125560
MethylacetophenonesColumns: 100 x 4.6 mm NUCLEOSHELL® PFP, 2.7 μm
250 x 4 mm NUCLEODUR® PFP, 5 μm 100 x 4.6 mm Kinetex® 2.6 μm PFP
Eluent: methanol – water (35:65, v/v)Flow rate: 1.5 mL/min, 3 mL/min, 1 mL/min, 1.5 mL/min Temperature: 35 °C Detection: UV, 254 nmPeaks:1. o-Methylacetophenone2. p-Methylacetophenone3. m-Methylacetophenone
0 10 20 30 40 min
1
NUCLEODUR® PFP, 1 mL/min
2
3
0 5 10 min
1
2
31
NUCLEOSHELL® PFP3 mL/min
2
3
NUCLEOSHELL® PFP, 1.5 mL/min
Kinetex® PFP, 1.5 mL/min
MN Appl. No. 125590
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NUCLEOSHELL® HILIC
NPC IC
HILIC
RPC
eluent
analyte
adso
rben
t
Key features: ·Basedoncore-shellparticle
technologyforfastandefficientHPLC · Ideal for reproducible and stable
chromatographyofhighlypolaranalytes
·Veryshortcolumnequilibrationtimes Technical characteristics:
Ammonium-sulfonicacidmodifiedsilica; pore size 90 Å, particlesize2.7μm; carboncontent1.3%; pHstability2–8.5;suitableforLC/MS
Recommended application: Hydrophiliccompoundssuchas
organic polar acids and bases, polar natural compounds, nucleosides, oligonucleotides, amino acids, peptides, water soluble vitamins
NUCLEOSHELL® HILICHydrophilic interaction chromatography (HILIC)is a separation technique using polar stationaryphases and organic-aqueous mobile phases. Aminimumwater content of at least 2% is indis-pensabletoprovideapermanentwaterlayerbe-tween the adsorbent surface and the organic frac-tion of the mobile phase. The sample molecules becomeseparatedinapartitionchromatography,inwhichpolaranalytesaremorestronglyretainedthanneutral,lesshydrophiliccompounds.Conse-quently,increasingtheaqueouspartinthemobilephase will diminish retention of the polar sample constituents.InthiswayHILICbehavesinversetoclassicalRPchromatography.Theparticularreten-tionprofileofHILICenablesthechromatographyof very polar and often small molecules, whichwon’t show any retention on C8 or C18 reversed phases.
Ultra-fast separations at moderate back pressureNUCLEOSHELL® HILIC is a core-shell technologybasedstationaryphasewithacovalentlybonded3-N,N-dimethylaminopropanesulfonicacidligand(pat.pend.).Thebetaine characterof the strongion-exchangerresultsinfullchargebalancingandfacilitatesfastequilibrationtimes.
CH3
CH3
SiO
2
+ N SO3 –
Good separation of polar compounds like the physiologically important substances creatineand creatinine can be achieved on NUCLEOSHELL® HILICaswellasonNUCLEODUR®HILIC,1.8µmatsimilar retention, but much lower back pressure.
Separation of creatine and creatinineColumns: 50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μm
50 x 4 mm NUCLEODUR® HILIC, 1.8 μmEluent: acetonitrile – 10 mmol/L ammonium acetate
pH 4.0 (90:10, v/v)Flow rate: 1.7 mL/minPressure: 129 bar
180 barTemperature: 25 °CDetection: UV, 210 nm Peaks:1. Creatinine2. Creatine
1
2
0.0 0.2 0.4 0.6 0.8 1.0min
MN Appl. No. 124990
13www.mn-net.com
NUCLEOSHELL®
Separation of catecholaminesColumns: 100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μm
100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μm 250 x 4 mm NUCLEODUR® HILIC, 3 μm
Eluent: acetonitrile – 100 mmol/L ammonium formate pH 3.2 (80:20, v/v)Flow rate: 4 mL/min, 1 mL/min, 1 mL/minPressure: 395 bar, 95 bar, 116 barTemperature: 25 °CDetection: UV, 280 nmPeaks:1. DOPAC2. Serotonin3. Dopamine4. Epinephrine5. Norepinephrine6. DOPA7. DOPS
0 5 10 min
1
2
3
4
5
6
7
12 min12 mL1
2
3
4
56
7
4.5 min4.5 mL
1
1 min4 mL
2
3
4
56
7
Core-Shell {Fully porous
(solventconsumptionper run)
MN Appl. No. 125440
Thechromatogramsshowthemethodtransferfromafullyporous3µmHILICphaseto2.7µmcore-shellsilicawithequalselectivityfeatures.Runtimehasbeencutdownto1min.Columnbackpressureremainsmodest<400bar,whilesolventdemandisreducedtolessthan35%.
NUCLEOSHELL® HILIC provides stable and reproducible chromatogra-phy, comprising all the benefits of a state-of-the-art core-shell silica.
Core-shell silica:
separation in 1 min
pressure < 400 bar
www.mn-net.com14
ApplicationsSeparation of ketones
Columns: 50 x 3 mm NUCLEOSHELL® RP 18, 2.7 μm 50 x 2 mm NUCLEODUR® C18 Gravity, 1.8 μm 125 x 2 mm NUCLEODUR® C18 Gravity, 5 μm
Eluent: acetonitrile – water (60:40, v/v)Flow rate: 4 mL/min, 1.25 mL/min, 0.33 mL/min Pressure: 540 bar, 774 bar, 89 barTemperature: 25 °CDetection: UV, 254 nm Injection: 1 μL, 1 mg/mL of each compound in eluentPeaks:1. Acetophenone2. Eugenol3. Propiophenone4. Butyrophenone5. Benzophenone6. Valerophenone
0 1 2 3 min
1
2
3
45 6
12
3
4
56
12
3
45
6
1
2
3
4
56
1
2
3
45
6
NUCLEOSHELL®
0.0 0.2 0.4 min
NUCLEODUR®
1,8 μm
MN Appl. No. 124920
Tricyclic antidepressantsColumns: 50 x 4.6 mm NUCLEOSHELL® RP 18, 2.7 μm
250 x 4.6 mm fully porous C18, 5 μmEluent: methanol – acetonitrile – 25 mmol/L KH2PO4
pH 7 (22.5:22.5:55, v/v)Flow rate: 2 mL/min, 1.3 mL/minPressure: 224 bar, 190 barTemp.: 40 °CDetection: UV, 220 nm
0 50 100 150 min
1
2
3
4 5
6 78
9
0 5 10 15 min
1
18 min36 mL
180 min234 mL
2
3
4
5
6 78
9
20
Peaks:1. Protriptyline2. Desipramine3. Maprotiline4. Nortriptyline5. Doxepin6. Imipramine7. Amitriptyline8. Clomipramine9. Trimipramine
MN Appl. No. 125420
time sav
ing
90 %
eluent s
aving
85 %
15www.mn-net.com
NUCLEOSHELL®
Acidic pharmaceuticalsColumns: 50 x 4 mm NUCLEOSHELL® RP 18, 2.7 μm
150 x 4 mm fully porous C18, 5 μmEluent: A) acetonitrile, B) 25 mmol/L KH2PO4 pH 7,
25–40 % A in 2.2 min, 25–40 % A in 20 minFlow rate: 1.5 mL/min, 0.5 mL/minPressure: 219 bar, 92 barTemp.: 20 °CDetection: UV, 215 nm Peaks:1. Ketoprofen2. Fenoprop3. Fenoprofen4. Flurbiprofen
5. Ibuprofen7. Carprofen8. Diclofenac9. Meclofenamic acid
0 1 2 min
12
34
5
6
7
8
2.2 min3.3 mLRs (2, 3) = 1.69
0 5 10 15 min
1
2
34
5
6
7
820 min10 mLRs (2, 3) = 1.70
MN Appl. No. 125430
Separation of steroidsColumns: 50 x 3 mm NUCLEOSHELL® RP 18, 2.7 μm
125 x 3 mm NUCLEODUR® C18 Gravity, 3 μmEluent: A) acetonitrile, B) water
30–80 % A in 1 min (0.4 min 80 % A) 30–80 % A in 5 min (2 min 80 % A)
up to 90 % time saving
66 % solvent saving
Flow rate: 2 mL/min 1 mL/min
Pressure: 350 bar 280 bar
Temperature: 25 °C Detection: UV, 240 nmInjection: 1 μL, 1 mg/mL of each compound in eluent
0 21 3 4 5 min
32
1 4
5
6
7
8 93
4
5
61.4 min2.8 mL
7 min7 mL
2
1 7
8 9
Peaks:1. Estriol2. Prednisolone3. Cortisone4. Estradiol5. Testosterone6. Estrone7. 6a-Methyl-11b-hydroxyprogesterone8. 6a-Methyl-17a-hydroxyprogesterone9. Progesterone
MN Appl. No. 124930
www.mn-net.com16
Non-steroidal anti-inflammatory drugsColumns: 50 x 4.6 mm each
NUCLEOSHELL® RP 18, 2.7 μm Ascentis® Express C18
Eluent: acetonitrile – 20 mmol/L KH2PO4 pH 2.5 (40:60, v/v)
Flow rate: 2.5 mL/minPressure: 268 bar, 281 barTemperature: 22 °CDetection: UV, 230 nmInjection: 1 μL, 1 mg/mL of each compound in eluent
0 1 2 3 min
3
1
2
6
45 8
Rs = 1.85 Rs = 1.59
9 10 1112
7
Rs = 1.25 Rs = 1.14
Peaks:1. Acetylsalicylic acid2. Sulindac3. Piroxicam4. Suprofen5. Tolmetin6. Naproxen7. Diflunisal8. Fenoprofen9. Flurbiprofen10. Niflumic acid11. Indomethacin12. Ibuprofen
MN Appl. No. 124970
Steviol glycosidesColumns: 150 x 4.6 mm
NUCLEOSHELL® RP 18, 2.7 μmEluent: acetonitrile – 10 mmol/L NaH2PO4 pH 2.6
(32:68, v/v)Flow rate: 1.0 mL/minTemperature: 40 °CDetection: UV, 210 nmInjection: 5 μL
0 2 4 6 min
2
3
5
1 4 6 7 8 9
Peaks:1. Rebaudioside D2. Rebaudioside A3. Stevioside4. Rebaudioside F5. Rebaudioside C6. Dulcoside A7. Rubusoside8. Rebaudioside B9. Steviolbioside
MN Appl. No. 125621
Phosphonic acid herbicidesColumns: 100 x 2 mm
NUCLEOSHELL® RP 18, 2.7 μmEluent: A) acetonitrile,
B) 50 mmol/L ammonium acetate; 5–50 % A in 3.7 min, 50–95 % A in 0.6 min (2 min 95 % A), 95–5 % A in 0.5 min (2 min 5 % A)
Flow rate: 0.5 mL/minTemperature: 30 °CDetection: MSInjection: 5 μLPeaks:1. Glyphosate (167 ng/mL)2. Glufosinate (16.7 ng/mL)3. AMPA (167 ng/mL)
GlufosinatetR = 2.34 min
GlyphosatetR = 2.09 min
AMPAtR = 2.88 min
2
1
3
2
3 min
Courtesy of KUDZU SCIENCE, Illkirch, France
MN Appl. No. 126110
good
selectiv
ity
and re
solution
Applications
17www.mn-net.com
NUCLEOSHELL®
β-Lactam antibioticsColumn: 50 x 2 mm
NUCLEOSHELL® Phenyl-Hexyl, 2.7 μmEluent: methanol – 10 mmol/L ammonium formate,
pH 3 (50:50, v/v)Flow rate: 0.45 mL/min Temperature: 40 °C Detection: MSInjection: 1 μLPeaks:1. Cefoxitin2. Cloxacillin3. Nafcillin4. Dicloxacillin
0 1 2 3 4 min
1
2
3
4
MN Appl. No. 126030
AntihistaminesColumn: 100 x 3 mm
NUCLEOSHELL® Phenyl-Hexyl, 2.7 μmEluent: A) methanol,
B) 10 mmol/L ammonium formate, pH 2.8; 17.5 % A (2.5 min) → 65 % A in 1.5 min → 75 % A in 1.5 min (4.5 min 75 % A)
Flow rate: 0.6 mL/min Temperature: 40 °C Detection: MSInjection: 0.5 μLPeaks:1. 4-Acetaminophenol2. Pseudoephedrine3. Codeine4. Chlorpheniramine5. Dextromethorphan
1 2 3 4 5 6 7 8 min0
1
23
4
5
MN Appl. No. 125950
BenzodiazepinesColumn: 50 x 2 mm
NUCLEOSHELL® Phenyl-Hexyl, 2.7 μmEluent: A) acetonitrile,
B) 20 mmol/L ammonium formate, pH 6.4; 25–55 % A in 10 min
Flow rate: 0.33 mL/min Temperature: 25 °C Detection: MSInjection: 2.5 μL
0 1 2 3 4 5 6 7 8 9 min
1
2
3
4 5
6
Peaks:1. Oxazepam2. Chlordiazepoxide3. Alprazolam4. Trazodone5. Nordiazepam6. Diazepam
MN Appl. No. 126140
www.mn-net.com18
ApplicationsPhthalates
Column: 100 x 3 mm NUCLEOSHELL® Phenyl-Hexyl, 2.7 μm
Eluent: A) acetonitrile – methanol (50:50, v/v), B) 0.1 % formic acid in water; 75 –98 % A in 3.5 min (2.5 min 98 % A)
Flow rate: 0.75 mL/min Temperature: 20 °C Detection: MSInjection: 0.5 μL
0 1 2 3 4 5 min
1
2
3
4
5
6
7
8
Peaks:1. Dimethyl phthalate2. Dipropyl phthalate3. Diphenyl phthalate4. Dibutyl phthalate5. Benzyl butyl phthalate6. Diheptyl phthalate7. Di-n-octyl phthalate8. Di-i-decyl phthalate
MN Appl. No. 126070
AflatoxinsColumn: 50 x 2 mm NUCLEOSHELL® PFP, 2.7 μmEluent: methanol – 10 mmol/L ammonium acetate
(45:55, v/v)Flow rate: 0.33 mL/min Temperature: 25 °C Detection: MSInjection: 0.1 ng eachPeaks:1. Aflatoxin G22. Aflatoxin G13. Aflatoxin B24. Aflatoxin B1
MN Appl. No. 125600
10 2 3 min
1
2
3
4
Beta- and dexamethasoneColumns: 50 x 4 mm NUCLEOSHELL® PFP, 2.7 μm
100 x 4.6 mm NUCLEODUR® PFP, 5 μmEluent: acetonitrile – water (20:80, v/v)Flow rate: 1.5 mL/min
1.3 mL/min Temperature: 30 °C Detection: UV, 260 nmPeaks:1. Betamethasone2. Dexamethasone
0 5 10 15 20 min
1
2
1
2
MN Appl. No. 125580
19www.mn-net.com
NUCLEOSHELL®
PhenolsColumns: 100 x 4.6 mm NUCLEOSHELL® PFP, 2.7 μm
100 x 4.6 mm NUCLEODUR® PFP, 5 μmEluent: acetonitrile + 0.1 % formic acid – 0.1 % formic acid
(35:65, v/v)
0 2 4 6 8 10 min
12
3
4
5
6
7 8
9
10
11
0 1 2 min
12
3
4
5
6
7 8
9
10
11
Flow rate: 4 / 1.3 mL/min Temperature: 35 °C Detection: UV, 280 nm
Peaks:1. o-Cresol2. m-Cresol3. 3,4-Dimethylphenol4. 3,5-Dimethylphenol5. 2,5-Dimethylphenol6. 2,6-Dichlorophenol
7. 2,3-Dichlorophenol8. 2,4-Dichlorophenol9. 3,4-Dichlorophenol10. 2,4-Dibromophenol11. 3,5-Dichlorophenol
MN Appl. No. 125570
Water-soluble vitaminsColumn: 100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent: A) acetonitrile – 100 mmol/L ammonium
acetate pH 3.2 (90:10, v/v), B) water; 4 % B (1 min) → 20 % B in 1.6 min (0.7 min 20 % B)
Flow rate: 2 mL/minPressure: 218 barTemperature: 25 °CDetection: UV, 260 nm Peaks:1. PABA (p-aminobenzoic acid)2. Nicotinamide3. Vitamin B6 (pyridoxine)4. Riboflavin5. Nicotinic acid6. Vitamin C (ascorbic acid)7. Vitamin B1 (thiamine)8. Folic acid9. Vitamin B12 (cyanocobalamine)
0 1 2 3 min
12
3 4
5
67
8
9
MN Appl. No. 125450
Anions and cationsColumn: 100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent: A) 30 mmol/L ammonium formate pH 3,
B) acetonitrile 80 % B (3 min) → 20 % B in 7 min
Flow rate: 1.5 mL/minPressure: 200 barTemperature: 40 °CDetection: CAD (Nebulizer: 35 °C) Peaks:1. Nitrate2. Chloride3. Potassium4. Sodium5. Phosphate6. Sulfate
0 1 2 3 4 5 6 min
12
3
4
5
6
MN Appl. No. 125460
www.mn-net.com20
ApplicationsMetformin
Columns: 50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μm 50 x 4 mm NUCLEODUR® HILIC, 1.8 μm
Eluent: acetonitrile – 10 mmol/L ammonium acetate pH 3.2 (75:25, v/v)
Flow rate: 3 mL/min 1.5 mL/min
Pressure: 202 bar 167 bar
Temperature: 25 °CDetection: UV, 218 nm
1
2
NUCLEOSHELL®
NUCLEODUR®
1
2
0.0 0.5 1.0 min
Peaks:1. Dicyandiamide2. Metformin
MN Appl. No. 125470
5-FluorouracilColumn: 50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent: acetonitrile – 10 mmol/L ammonium acetate
(95:5, v/v)Flow rate: 2.5 mL/minPressure: 119 barTemperature: 25 °CDetection: UV, 254 nmPeaks:1. 5-Fluorouracil2. Uracil
1
2
0.0 0.2 0.4 min
MN Appl. No. 125480
Analysis of an energy drinkColumn: 100 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent: acetonitrile – 100 mmol/L ammonium acetate
pH 5.0 (90:10, v/v)Flow rate: 1.7 mL/minPressure: 126 barTemperature: 35 °CDetection: UV, 254 nm Peaks:1. Caffeine2. Niacinamide3. Pyridoxine4. Benzoic acid5. Sorbic acid6. Riboflavin
1
2
34
5
6
0 1 min
MN Appl. No. 125010
good
selectiv
ity and
peak sh
ape
21www.mn-net.com
NUCLEOSHELL®
Acrylamide and analogsColumn: 50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent: acetonitrile – 0.1% formic acid in water
(98:2, v/v)Flow rate: 1.0 mL/minTemperature: 25 °CDetection: UV, 200 nm Injection: 1 μL, 1 mg/mL of each compound in eluent Peaks:1. Acrylamide2. Methacrylamide3. Methacrylic acid
3
2
1
0.0 0.5 1.0 min
MN Appl. No. 125160
NucleotidesColumn: 50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluents: A) acetonitrile; B) 100 mmol/L ammonium
acetate pH 5.35; 87.5–60 % A in 18 min Flow rate: 2.2 mL/minTemperature: 30 °CDetection: UV, 259 nm Injection: 1 μL, 1 mg/mL of each compound in eluent Peaks:1. Uridine2. Adenosine3. Cytidine4. Cyclic adenosine monophosphate5. Uridine monophosphate6. Adenosine monophosphate7. Inosine monophosphate8. Cytidine monophosphate
3 4
5
8
7
6
2
1
0 2 4 6 8 10 12 min
MN Appl. No. 125200
Amino acidsColumn: 50 x 4 mm NUCLEOSHELL® HILIC, 2.7 μmEluent: acetonitrile – 100 mmol/L ammonium
acetate pH 4.0 (80:20, v/v)Flow rate: 1.5 mL/minPressure: 105 barTemperature: 25 °CDetection: UV, 215 nm Peaks:1. Phenylalanine2. Phenylglycine3. Tyrosine4. Histamine
4
3
2
1
0.0 0.4 0.8 1.2 1.6 2.0 min
MN Appl. No. 125000
www.mn-net.com22
Packed columns · Ordering informationEC standard columns for analytical HPLC
Analyticalcolumnsystemmadeofstainless steel M8outerthreadsonbothendsCombination of sealing element and veryfine-meshedstainlesssteelscreen,PTFEringandfittingadapterColumnheadsSW12,withinnerthreadsM8x0.75andUNF10-32(=1/16”fitting)EC column hardware guarantees pressure stabilityof1200bar-henceECcolumnsaresuitableforU-HPLCapplications(ultrafastHPLC)andallmodernHPLCsystems.
Asscrew-onguardcolumnsystemwerec-ommend the Column Protection System used with EC guard column cartridges with 4mmlength(seenextpage).
EC analytical columns Allphases:poresize90Å,particlesize2.7µm; eluent in column CH3CN – H2O
Length→ 50 mm 100mm 150mm EC guard columns*
NUCLEOSHELL® RP 18, 2.7 µm octadecylmodification,multi-endcapped,7.5%C2mmID 763132.20 763134.20 763136.20 4x2mm:763138.203mmID 763132.30 763134.30 763136.30 4x3mm:763138.304mmID 763132.40 763134.40 763136.40 4x3mm:763138.304.6mmID 763132.46 763134.46 763136.46 4x3mm:763138.30
NUCLEOSHELL® Phenyl-Hexyl, 2.7 µm phenyl-hexylmodification,multi-endcapped,4.5%C2mmID 763732.20 763734.20 763736.20 4x2mm:763738.203mmID 763732.30 763734.30 763736.30 4x3mm:763738.304mmID 763732.40 763734.40 763736.40 4x3mm:763738.304.6mmID 763732.46 763734.46 763736.46 4x3mm:763738.30
NUCLEOSHELL® PFP, 2.7 µm pentafluorophenylmodification,multi-endcapped,~3%C2mmID 763532.20 763534.20 763536.20 4x2mm:763538.203mmID 763532.30 763534.30 763536.30 4x3mm:763538.304mmID 763532.40 763534.40 763536.40 4x3mm:763538.304.6mmID 763532.46 763534.46 763536.46 4x3mm:763538.30
NUCLEOSHELL® HILIC, 2.7 µm ammonium–sulfonicacidmodification,1.3%C2mmID 763332.20 763334.20 763336.20 4x2mm:763338.203mmID 763332.30 763334.30 763336.30 4x3mm:763338.304mmID 763332.40 763334.40 763336.40 4x3mm:763338.304.6mmID 763332.46 763334.46 763336.46 4x3mm:763338.30
*ECguardcolumnsrequiretheColumnProtectionSystemCartridgeHolderREF718966(seeright). ECcolumnsinpacksof1,guardcolumnsinpacksof3
23www.mn-net.com
NUCLEOSHELL®
Column Protection SystemInnovative and universal screw-on guard column holder systemSuitable for all analytical HPLC columns with 1/16” fittings
CartridgesfilledwithspecifiedNUCLEOSHELL®,NUCLEODUR®, and NUCLEOSIL® HPLC adsorbents
Idealprotectionforyouranalyticalmaincolumn →significantincreaseincolumnlifetime
Minimized void volume → suitable also for ultra fast HPLC
Special ferrules →pressurestabilityupto1034bar(15000psi)
Visualcontaminationcheck→in-timechanging of the guard column
Guardcolumnlength4mm,ID2mm(formaincolumnswith2mmID)orID3mm(formaincolumnswith3,4and4.6mmID)
UNIVERSALRPguardcolumnsavailableforall HPLC columns under RP conditions
Content of the Column Protection System
Description REF
Column Protection System 718966
Details Content
Cartridge Holder 1
Replacementcapillaries(0.12mmID) 2
Ferrules 3
Wrenches 2
Manual 1
Replacement parts for the Column Protection System • Ordering information
Description Pack of REF
Ferrules 5 718967ReplacementconnectorincludingO-ring 1 718968Stainlesssteelcapillaries0.12mmID,nutsandmetalferrules 3 718969Stainlesssteelcapillaries0.18mmID(forhigherflowrates),nutsandmetalferrules 3 718971Wrench(size12and14mm) 1 718970EC4/2UNIVERSALRPguardcolumn(formaincolumnswith2mmID) 3 728777.20EC4/2UNIVERSALRPguardcolumn(formaincolumnswith2mmID),valuepack 9 728778.20EC4/3UNIVERSALRPguardcolumn(formaincolumnswith3,4and4.6mmID) 3 728777.30EC4/3UNIVERSALRPguardcolumn(formaincolumnswith3,4and4.6mmID),valuepack 9 728778.30
Visual Contamination Check
The cartridge is fitted with a special filter membrane.
Ifthesilvermembraneiscontaminated(brightordarkdiscoloration),itisadvisable to replace the cartridge.
If the contaminants are colorless, replace the cartridge as soon as the pressure rises or the chromatographic performance decreases.
Trademarks: NUCLEOSHELL®,NUCLEODUR®(MACHEREY-NAGELGmbH&Co.KG,Germany),Ascentis®(Sigma-AldrichCo.,USA),Kinetex®(PhenomenexInc.,USA),Poroshell(AgilentTechnologiesInc.,USA) Imagecredits:©by-studio/Alexey(Fotolia.com)
www.mn-net.com
www.mn-net.comMACHEREY-NAGEL GmbH & Co. KG · Neumann-Neander-Str. 6–8 · 52355 Düren · GermanyFrance:MACHEREY-NAGEL EURLTel.: +33 388 68 22 68Fax: +33 388 51 76 88E-mail: [email protected]
Switzerland:MACHEREY-NAGEL AGTel.: +41 62 388 55 00Fax: +41 62 388 55 05E-mail: [email protected]
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EN ISO 9001: 2008CERTIFIED
USA:MACHEREY-NAGEL Inc.Tel.: +1 484 821 0984Fax: +1 484 821 1272E-mail: [email protected]
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