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Date post: 12-Jan-2016
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Objectives. To determine the properties and quality of the horse joint supplements Legend and Hyalovet by:. Using the rheometers to measure the viscosity and elasticity. Finding the intrinsic viscosity using capillary viscometers. Rheometers. Rate Control. Strain Control. Viscometer. - PowerPoint PPT Presentation
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Objectives To determine the properties and quality of the horse joint supplements Legend and Hyalovet by: Using the rheometers to measure the viscosity and elasticity. Finding the intrinsic viscosity using capillary viscometers.
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Page 1: Objectives

Objectives To determine the properties and

quality of the horse joint supplements Legend and Hyalovet by:

•Using the rheometers to measure the viscosity and elasticity.

•Finding the intrinsic viscosity using capillary viscometers.

Page 2: Objectives

Rheometers

Rate Control Strain Control

Page 3: Objectives

Viscometer Glass tubes that

can be used to calculate the intrinsic viscosity of polymer solutions by measuring the rate at which a liquid falls through the capillary at different dilutions.

Capillary

Timing Lines

Page 4: Objectives

Hyaluronic Acid

•Biopolyelectrolyte•High molecular weight•viscoelastic properties•costs 100,000 per kilogram

Sources of HA•rooster combs•micro-organisms•umbilical cords

Page 5: Objectives

Intrinsic Viscosities of HA Supplements in PBS

0.00

200.00

400.00

600.00

800.00

1000.00

1200.00

1400.00

1600.00

1800.00

2000.00

0.00E+00 2.00E-04 4.00E-04 6.00E-04 8.00E-04 1.00E-03 1.20E-03

Concentration HA (mg/ml)

(T-T

S)/

(TS

*C)

Trial 1 Hyalovet

Trial 2 Hyalovet

Trial 1 Legend

Trial 2 Legend

Linear (Trial 2Hyalovet)Linear (Trial 1Hyalovet)Linear (Trial 2Legend)Linear (Trial 1Legend)

IV = 534IV = 531(ml/g)

Intrinsic Viscosity = 1074 ml/g

IV = 991 ml/g

Page 6: Objectives

Moduli of Legend and Hyalovet

0.00

0.01

0.10

1.00

10.00

0.10 1.00 10.00

Frequency (hz)

Mod

uli (

Pa) Legend Elastic Modulus (Pa)

Legend Viscous Modulus (Pa)

Hyalovet Elastic Modulus (Pa)

Hyalovet Viscous Modulus (Pa)

Page 7: Objectives

Viscosity of Legend and Hyalovet

0.01

0.10

1.00

0.10 1.00 10.00 100.00 1000.00

Shear Rate (1/s)

Vis

cosi

ty (

Pas

)

Legend Viscosity

Hyalovet Viscosity

Page 8: Objectives

They Came to Tame the Lame

Page 9: Objectives

Conclusions The experiments performed on the

rheometer showed similar readings for the flow of Legend and Hyalovet

Legend and Hyalovet showed the same viscous and elastic moduli.

The intrinsic viscosity of Hyalovet is twice that of Legend which shows that Hyalovet has a larger molecular weight.


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