Operation Manual - Cole-Parmer...W2t functionality normally found on ultracentrifuges. This advanced...

Operation Manual

Cole-Parmer® MPR115 Advanced Multipurpose Refrigerated and MP115 Advanced Multipurpose Ambient Centrifuges with Automatic Rotor Recognition and Touchscreen LCD

Models 17406-10, -15, -40, -45

CE

1065z75_MAN_Rev.1

©2016 Cole-Parmer Instrument Company, LLC

Table of Contents

Preface 1

Introduction 1

Unpacking 1

Precautions 1

Setup Conditions 2

Preparation 2

Hazardous Substances 2

Operation & Symbols 3

Features & Advantages 4

Technical Specifications – Centrifuge 5

Technical Specifications – Rotors 6

Transport & Set Up 12

Making Sure the Rotor is Balanced 14

Installation/Fixation for Fixed-Angle Microtube Rotor 15

Installation/Fixation for Fixed-Angle Rotor 16

Installation/Fixation for Swing-Out Rotors 17

Programming & Operation 20

Maintenance & Care 26

Troubleshooting 30

Contact Information 32

1

Preface

Before starting to use the Cole-Parmer series of multipurpose centrifuges, read through this instruction manual carefully and follow the instructions. The information contained in this instruction manual is the property of Cole-Parmer Instrument Company, LLC; it is forbidden to copy or pass on this information without explicit approval. Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the warranty.

This centrifuge is a laboratory product used to separate substance mixtures of different densities. This centrifuge should be operated by trained personnel only.

Introduction

Cole-Parmer series of advanced benchtop centrifuges (MPR115 refrigerated models 17406-10 and -15; MP115 ambient models 17406-40 and -45) offers rugged construction without sacrificing reliable performance. Extra-thick stainless steel chamber and zinc-coated alloy and steel frame increase durability for inorganic separations requiring strong solvents. Industrial-grade inverter drive (VFD) and brushless motor provide a long service life. User-accessible service sections allow onsite maintenance.

Refrigerated models feature a highly accurate PID controller allows better overall temperature control, achieving the desired set temperature. In addition, the large, efficient CFC-free compressor uses less power and lasts longer.

Automatic rotor recognition technology provides safety and convenience when different rotors are used. The installed rotor is recognized at the beginning of a run and is automatically limited to its rated maximum speed. Rotors and accessories are not included; please see pages 6-11 or contact Cole-Parmer.

Bright blue touch-screen LCD is simple to navigate. Programmable memory supports 108 programs including acceleration and deceleration rates, allowing you to quickly perform routine tasks, or use the W2t functionality normally found on ultracentrifuges. This advanced centrifuge is ideal for your research needs, offering reliable and repeatable separations.

Unpacking

Check individual parts against the list of items below. If anything is missing or damaged, please contact Cole-Parmer immediately.

1. Multipurpose refrigerated centrifuge

2. Rotor installation kit (rotor is not included; see pages 6-11)

3. Power cord

4. User manual

Precautions

Observe the safety instructions. Not following these instructions can cause damage. The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with fatal consequences.

The centrifuge should be operated by trained personnel only.

In order to ensure safe operation of the Cole-Parmer centrifuge, general safety regulations must be followed: refer to the regulations in your country.

2

Setup Conditions

Plug the centrifuge only into sockets which have been properly grounded.

Turn off the centrifuge at the main switch. The mains plug must be freely accessible at all times. Press the STOP key to shut the centrifuge down.

Pull out the power supply plug or disconnect the power supply in an emergency.

Maintain a radius of at least 12 in. (30 cm) around the centrifuge.

Do not place any dangerous substances within the security zone.

Set up in a well-ventilated environment, place on a level and rigid surface with adequate load-bearing capacity.

Preparation

It is the general obligation of the operator to make sure that the proper protective clothing is used. Refer to the "Laboratory Biosafety Manual" of the World Health Organization (WHO) and the regulations in your country.

Do not touch the mechanical components of the rotor and do not make any changes to the mechanical components.

Do not touch the electronic components of the centrifuge or alter any electronic or mechanical components.

Use only with rotors which have been properly installed. Follow the instructions on pages 15-19.

Do not use rotors which show any signs of corrosion and/or cracks. Contact your supplier if the centrifuge and rotors are in any conspicuous condition, e.g. showing signs of corrosion and/or cracks.

Use only with rotors which have been loaded properly.

Never overload the rotor.

Always balance the samples.

Use only rotors and accessories for this centrifuge which have been approved by the supplier. Exceptions to this rule are commercially available glass or plastic centrifuge tubes, provided they have been approved for the speed or the RCF value of the rotor.

Make sure the rotor is locked properly into place before operating the centrifuge.

Implement of special measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it is running.

If used for foodstuffs machinery, for cosmetics or pharmaceutical products, only use closed or aerosol-tight containers for centrifugation.

Hazardous Substances

Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and vessel have to be cleaned carefully.

Do not centrifuge explosive or flammable materials or substances which could react violently with one another.

The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone environment.

Do not centrifuge inflammable substances.

Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions.

3

&

If centrifuging any hazardous materials, mind the “Laboratory Biosafety Manual” of the WHO. When centrifuging microbiological samples from the Risk Group II, aerosol-tight biological seals must be used.

For materials in a higher risk group, extra safety measures have to be taken. If toxins or pathogenic substances have gotten into the centrifuge or its parts, appropriate disinfection measures have to be taken. See "Disinfection" on page 27. Highly corrosive substances which can cause material damage and impair the mechanical stability of the rotor should only be centrifuged in corresponding protective tubes. If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.

Operation & Symbols

Never use the centrifuge if parts of its cover panels are damaged or missing.

Never start the centrifuge when the door is open.

Do not move the centrifuge while it is running.

Do not lean on the centrifuge.

Do not place anything on top of the centrifuge during a run. This applies also for the rotor landing pad.

Never open the door until the rotor has come to a complete stop and this has been confirmed in the display.

Emergency door release may be used in emergencies only to recover samples from the centrifuge, e.g. during a power failure (see section "Mechanical Emergency Door Release” on page 30).

Do not open the centrifuge, while it is running.

In any case of mechanical breakdown rotor failure, like burst of the rotor, breaking bottles or shaft crack, the centrifuge is not aerosol-tight.

In case of rotor failure, the centrifuge can be damaged. Leave the room immediately.

Symbols used on the centrifuge:

This symbol refers to general hazards.

CAUTION! means that material damage could occur.

WARNING! means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards.

Observe the information contained in the instruction manual to keep yourself and your environment safe.

This symbol refers to information on hazards, described within this manual.

4

Features & Advantages

Built to last, strong construction yet offering a sleek modern design that will fit into any modern laboratory. No compromise on quality—only the best components are used in the manufacturing process.

User Features Advantages

Large, bright blue LCD with touch control Clarity and ease of use

Automatic rotor recognition Safe operation of multiple rotors

W2t functionality plots speed against time True sample repeatability

10 acceleration rates, 10 deceleration rates Sample assistance

108 program memory (12 pages of 9) Multiple departments and users

Timer 0 to 9999 minutes and Hold in 1-sec increments Total flexibility

Pulse short run Fast pelleting

Run in speed (rpm) or RCF (G) in 1-rpm increments Accuracy

Sound <60 dB Quiet

Orientation acceleration rate Prevents initial sample side deposits

Refrigerated models are PID controlled Excellent temperature accuracy

Mechanical Features Advantages

Extra-thick stainless steel bowl Easy to clean and rust free

Port to lid Tachometer speed certification

Alloy and steel frame with composites Strong light and quiet

World-leading industrial-grade Inverter Reliability and strength

Quality European brushless motor Quiet, cool, and reliable

High technology airflow (ambient models) Cooler running

Safety Features Advantages

Multipoint lid locking Lid safety

Emergency lid-release Power failure release

Lid gas strut Lid dropping safety

Lid lock detection Lid must be shut in order to operate

Imbalance detection Easy “eye” volume loading

Overspeed sensor Safe detection of speed

Set inverter values Electronic safety of speed

Barrier ring Extra metal protection of chamber

Motor overheat sensor Safe motor protection

Note: All centrifuges have user-accessible service sections to access all safety parameters

5

Technical Specifications – Centrifuge

Model 17406-10 (display indicative only)

Speed: 500 to 15,000 rpm, in 1 rpm steps

RCF max: 22,000 RCF x g

Max capacity: 1 L

Temperature (refrigerated models): –9 to 40°C (15.8 to 104°F), PID controlled to ±1°C

Acceleration rates: 10 programs

Deceleration rates: 10 programs

Memory: 108 programs

Timer: 0 to 9999 minutes and Hold, in 1 second steps

Dimensions (W x H x D): Refrigerated models: 17.7 x 12.4 x 25 in. (45 x 31.5 x 63.5 cm) Ambient models: 15.7 x 12.2 x 19.7 in. (40 x 31 x 50 cm)

Weight without rotor: Refrigerated models: 137 lb (62.2 kg) Ambient models: 71 lb (32 kg)

Power: Refrigerated: 110 VAC, 60 Hz (model 17406-10); 230 VAC, 50 Hz (model 17406-15) Ambient: 110 VAC, 60 Hz (model 17406-40); 230 VAC, 50 Hz (model 17406-45)

Rotors included: None; see pages 6-11.

6

Technical Specifications – Hematocrit Rotor

Catalog number 17406-78 (not included)

Rotor model BRK5401

Description Fixed angle, 24 x capillary & 12 x 2 mL tubes

Locking lid Included

Tube size max 2 x 75 mm & 11 x 40 mm

Minimum speed 500 rpm

Maximum speed 12,000 rpm

Maximum RCF x g 13,500

Radius max (cm) 8.5

Sample tube angle 0 & 60

Acceleration time 30 sec

Deceleration time 30 sec

Autoclaveable 121°C (10) (frequency)

7

Technical Specifications – Microtube Rotors* (max 15,000 rpm)

Rotors

Lid

Catalog number 17406-03 17406-28 17406-29 17406-31 (not included) (not included) (not included) (not included)

Rotor model BRK5424 BRK5436 BRK5448 BRK5494

Description Fixed angle, Fixed angle, Fixed angle, Fixed angle, polypropylene, polypropylene, polypropylene, polypropylene, 24 x 2 mL 36 x 0.5 mL 48 x 0.2 mL 4 x PCR strips

High-dome PC lid Included Included Included Included

Tube size max 11 x 50 mm 8 x 30 mm 6 x 40 mm 8 per strip

Minimum speed 500 rpm 500 rpm 500 rpm 500 rpm

Maximum speed 15,000 rpm 15,000 rpm 15,000 rpm 15,000 rpm

Maximum RCF x g 22,000 22,000 22,000 22,000

Radius max (cm) 8.75 8.75 8.75 8.75

Sample tube angle 45 45 45 45

Acceleration time 20 sec 20 sec 20 sec 20 sec

Deceleration time 20 sec 20 sec 20 sec 20 sec

Autoclaveable 121°C (20) 121°C (20) 121°C (20) 121°C (20) (frequency)

Minimum temperature 4°C 4°C 4°C 4°C for refrigerated models

Reducers

Catalog number 17406-37 (set of 24)

Model RS04 [0.2-0.4ml]

Description Description Max tube size 6 x 30 mm

*Additional rotors, reducers, and adapters are available; contact Cole-Parmer for details.

8

Technical Specifications – Rotors* (max 6000 rpm)

Rotors

Catalog number 17406-79 17406-09 17406-07 (not included) (not included) (not included)

Rotor model BRK5324 BRK5308 BRK5100

Description Fixed angle, polypropylene, Fixed angle, polypropylene, Fixed angle, polypropylene, 24 x 15 mL 8 x 50 mL 6 x 100 mL

Lid None None None

Tube size max 17 x 120 mm 8 x 30 mm 6 x 40 mm

Minimum speed 500 rpm 500 rpm 500 rpm

Maximum speed 6000 rpm 6000 rpm 6000 rpm

Maximum RCF x g 4800 4800 4800

Radius max (cm) 12 12 12

Sample tube angle 30 30 30

Acceleration time 35 sec 35 sec 35 sec

Deceleration time 35 sec 35 sec 35 sec

Autoclaveable (frequency) 121°C (20) 121°C (20) 121°C (20)

Minimum temperature 4°C 4°C 4°C for refrigerated models

Reducers

Catalog number 17406-32 (set of 4) 17406-34 (set of 4)

Model RM05 [5ml] RM15 [15ml]

Description Description Max tube size 13 x 80 mm Max tube size 17 x 120 mm




*Additional rotors, reducers, and adapters are available; contact Cole-Parmer for details.

9

Technical Specifications – Rotors* (max 10,000 rpm)

Rotors

Catalog number 17406-13 17406-41 (not included) (not included)

Rotor model BRK5212 BRK5206

Description Fixed angle, aluminum, Fixed angle, aluminum, 12 x 15 mL 6 x 50 mL

Lid None None

Tube size max 17 x 120 mm 30 x 120 mm

Minimum speed 500 rpm 500 rpm

Maximum speed 10,000 rpm 10.000 rpm

Maximum RCF x g 10,600 10,600

Radius max (cm) 9.5 9.5

Sample tube angle 30 30

Acceleration time 35 sec 35 sec

Deceleration time 35 sec 35 sec

Autoclaveable (frequency) 121°C (30) 121°C (30)

Minimum temperature 4°C 4°C for refrigerated models

Reducers







*Additional rotors are available; contact Cole-Parmer for details.

10

Technical Specifications – Swing-Out Rotors*

Rotors

Catalog number 17406-42 Special order 17406-06 (not included) (not included)

Rotor model BRK1000 BRK5508 BRK5530

Description Swing out, aluminum, 4 x 250 mL Swing out, 8 x 15 mL Swing out, 4 x microtiter plates (piggy back)

Buckets* (set of 4) Required (# 17406-12) Included None

Sealed lids (set of 4) Available (# 17406-43) None None

Tube size max 62 x 110 mm 17 x 125 mm 85 x 130 mm




Radius max (cm) 14.6 14.6 12.5





Minimum temperature 4°C 4°C 4°C for refrigerated models

Tube Adapters


Model AR615

Description Tube type Plain, no cap, round bottom, 15 mL, 17 x 100 mm


Model AR650

Description Tube type Plain, no cap, round bottom, 50 mL, 34 x 100 mm


Model AF615

Description Tube type Falcon with cap, pointed bottom, 15 mL, 17 x 120 mm


Model AN0610

Description Tube type Nalgene™/Oakridge, round bottom, 10 mL, 16 x 80 mm


Model AN0650

Description Tube type Nalgene™/Oakridge, round bottom, 50 mL, 29 x 107 mm

*Additional rotors are available; contact Cole-Parmer for details.

11

Technical Specifications – Cytology Rotors*

Using centrifugal force to separate and deposit monolayer of cells onto slides whilst maintaining integrity

within a clearly defined area from any liquid matrix. Cyto centrifugation also constructively flattens cells for

excellent nuclear presentation. Applications include cytology, histology, hematology, immunochemistry,

serology, and microbiology. Offering samples from 0.1 mL to 6 mL and an extensive range of accessories.

Rotors

Catalog number 17406-71 17406-72 17406-73 (not included) (not included) (not included)

Rotor model 4420 4430 4460

Description 4 x 0.2 to 6 mL 8 x 0.2 to 6 mL 12 x 0.2 to 6 mL

Lid Included Included Included

Tube size max Single or double Single or double Single or double




Radius max (cm) 12 12 12





*Cytology rotors include rotor, sealed lid, clips, 24 holders with filter card and 100 slides.

Sample Holders

Catalog number Special order (set of 24) Catalog number Special order (set of 24)

Model 4446 Model 4600

Description Description Double sample holder Description Double sample holder with card (up to 1 mL) with card (up to 6 mL)

Catalog number Special order (set of 24) Catalog number Special order (each)

Model 4444 Model 4462

Description Description Single sample holder Description Stainless steel clip with card (up to 1 mL)

12

Transport & Set Up

Location

The centrifuge should only be operated indoors. The set-up location must fulfill the following requirements:

• A safety zone of at least 12 in. (30 cm) must be maintained around the centrifuge.

• People and hazardous substances must be kept out of the safety zone while centrifuging.

• The supporting structure must be stable and free of resonance.

• The supporting structure must be suitable for horizontal setup of the centrifuge.

• The centrifuge should not be exposed to heat and strong sunlight.

• The set-up location must be well-ventilated at all times.

WARNING!

UV rays reduce the stability of plastics. Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.

Transporting the Centrifuge

• Due to its weight, the centrifuge should be carried by several people.

• Always remove the rotor before moving the centrifuge.

• Always lift the centrifuge on both sides. Never lift the centrifuge by its front or back panel.

• Transport the centrifuge upright and if at all possible in its packaging.

NOTICE

Store the original centrifuge packaging. Contact a shipping company for the transport or inform the customer service.

Always remove the rotor before moving the centrifuge. If you do not remove the rotor, you might damage the centrifuge drive or centrifuge spindle.

Aligning the Centrifuge

The horizontal alignment of the centrifuge must be checked every time after moving it to a different location. The supporting structure must be suitable for horizontal setup of the centrifuge.

CAUTION!

If the centrifuge isn't leveled, imbalances can occur and the centrifuge can be damaged. Do not place anything under the centrifuge feet to level the centrifuge.

13

Mains Connection

1. Turn off the power supply switch located on the left side of the centrifuge (press "0").

Power supply switch

2. Plug the centrifuge into grounded electrical sockets only.

3. Check whether the cable complies with the safety standards of your country.

4. Make sure that the voltage and frequency correspond to the figures on the rating plate.

5. Establish the connection to the power supply with the connecting cable.

Storage

WARNING!

When removing the centrifuge and accessories from use you have to clean and if necessary disinfect or decontaminate the entire system. If in doubt, contact the supplier.

• Before storing the centrifuge and accessories, it must be cleaned and if necessary disinfected and decontaminated.

• Store the centrifuge in a clean, dust-free location.

• Be sure to place the centrifuge on its feet.

• Avoid direct sunlight.

Shipping the Centrifuge

Before shipping the centrifuge, please bear the following in mind:

• The centrifuge must be clean and decontaminated.

• You must provide proper documentation confirming the decontamination.

• If in doubt, contact the Cole-Parmer.

14

Making Sure the Rotor is Balanced

In centrifugation, it is vitally important that rotors are balanced correctly. Any slight imbalance when weighing or placing your sample in the rotor could be exaggerated once the centrifuge is spinning.

Please note the examples shown below of correct loading of samples:

Incorrect Incorrect Correct

15

Installation/Fixation for a Fixed-Angle Microtube Rotor

Microtube rotor includes:

High-dome polycarbonate lid

M6 washer

Threaded fastener

Allen key

Step 1. Locate threaded fastener into the Step 2. Place the washer onto rotor screw. three interlocking pin holes on the rotor. Insert screw and washer into central hole of rotor.

Step 3. Hand tighten with Allen key. Step 4. To fasten the rotor lid, place lid on threaded fastener and hand tighten using the rotor knob.

CAUTION!

Please ensure that the fixing installation is checked and hand-tightened on a regular basis.

Failure to follow these instructions could cause serious damage to the centrifuge and possible injury to persons nearby and would make the warranty invalid.

16

Installation/Fixation for a Fixed-Angle Rotor

Installation kit includes:

M6 washer

Rotor fixing screw, M6 x 20 mm

Allen key

Step 1. Place the rotor on the Step 2. Place the washer over

shaft of the motor. the central hole on the rotor.

Step 3. Place the rotor fixing screw Step 4. Hand tighten the rotor

into the central hole of the rotor. fixing screw with the Allen key.

CAUTION!



17

Installation/Fixation for Swing-Out Rotor, 4 x 250 mL


M6 washer


Allen key

Step 1. Place the rotor on the shaft of motor. Step 2. Place the M6 washer over the

central hole on the rotor.

Step 3. Place the rotor fixing screw into Step 4. Hand tighten with Allen key.

the central hole of the rotor.

Step 5. Ensure buckets are fully inserted

before use

CAUTION!



18

Installation/Fixation for Swing-Out Rotor, 8 x 15 mL


M6 washer


Allen key

Step 1. Place the rotor on the shaft of motor. Step 2. Place the M6 washer over the central hole on the rotor.

Step 3. Place the rotor fixing screw into Step 4. Hand tighten with Allen key.

the central hole of the rotor.

CAUTION!



19

Installation/Fixation for Swing-Out Rotor, 4 x microtiter plates


M6 washer


Allen key

Step 1. Place the rotor on the shaft of motor. Step 2. Place the M6 washer over the

central hole on the rotor.

Step 3. Place the rotor fixing screw into Step 4. Hand tighten with Allen key. the central hole of the rotor.

CAUTION!



20

Programming & Operation

Creating a Program

Retrieving a Set Program

Display shown when Page key pressed Display shown at turn on Display shown when program key pressed

1. Press program key (book emblem top right).

2. Press Page number box. 3. Select page number required 1 to 9 pages with 12 names per page (a total of 108 programs can be stored).

4. Press Set Name box. 5. Key in name required (up to 14 alphanumeric characters).

6. Press Tick mark if correct.

1. Press program key (book emblem top right).

2. Press Page number box and select page number required.

3. Press and release to select

desired program name.

Note: These centrifuges can store up to 108 programs, conveniently grouped into 9 pages with up to 12 names

available per page. Each name can consist of up to 14 alphanumeric characters.

21

Programming the Rotor

Display shown after pressing Rotor box Display shown after pressing Set Parameters Display shown after pressing Tick on

name settings

Display shown after selecting rotor

1. Press Set Parameters. 3. Select rotor required by pressing down and releasing.

2. Press Rotor box.

4. Correct rotor should be shown in Rotor Display.

5. Press Bz to turn off end of run buzzer (white square indicates “on”).

22

Programming the Time

Programming the Speed

Display shown when time box key pressed

Display shown when Time Numerical box is shown

Display shown when RPM pressed Display shown after selecting RPM

Display shown after pressing numerical box

1. Press Time box.

3. Press Numerical box next to Time box.

2. Select and press desired timer type:

4. Select Time required in minutes and seconds (Hold and Pulse cannot be changed); or select RCFt

number from previous run).

Time = 0 to 9999 minutes

RCFt = See W2t document

for explanation

Pulse = Timer counts up in

seconds when Start button is held down (90 seconds max)

Hold = Timer is held on until

Stop button is pressed

5. Press Tick mark after selection.

1. Press RPM box. 3. Press Numerical box next to RPM box.

2. Select RPM (speed) or RCF (G Force)

4. Set Speed (RPM) or RCF in numerical form.


23

Setting Acceleration and Deceleration (Brake) Rates

Display shown when Acc pressed

Display shown after selecting Acc

1. Press Acc box.

3. Press number required and release.

2. Select Rate 1 to 10

7. Press Tick mark after checking all parameters are correct.

4. Press Dec box. 5. Select Rate 1 to 10

6. Press number required and release.

24

Programming the Temperature

Before Operation

If the centrifuge has been running for some time, over an hour, at the same temperature, then equilibration is not necessary. However if it has just been turned on, then we recommend the following for best performance:

For Temperatures Below Ambient or 18°C (4 to 18°C)

Turn on the unit. Select your temperature, and make sure the rotor is in position and firmly fixed. Close the centrifuge lid by pressing both sides down firmly. Wait at least 20 minutes. While waiting, place your samples with tubes in refrigerated unit at the desired temperature.

After your set temperature has been reached, use the Precondition mode by pressing down the Function button and Start/Stop button together (see display at right). Two dots will appear on the display showing that the precondition mode has been accessed; then press Function button to start. Precondition mode runs the centrifuge at half programmed speed to ensure full mixing of the chamber air and therefore equilibrates all areas of the rotor and parts for maximum efficiency.

Once this has finished, lift the lid and load samples in position. See page 14 for correct loading procedures.

For Temperatures Above Ambient or 18°C (18 to 40°C)

Turn on the unit. Set the temperature to 4°C. Make sure the rotor is in position and firmly fixed. Close the centrifuge lid by pressing both sides down firmly. Wait at least 20 minutes. This method allows the gases to warm up for higher temperature use. Once 4°C has been reached, set your desired temperature above ambient. While waiting, place samples with tubes in a heated unit at the desired temperature.

After your set temperature has been reached, use the Precondition mode by pressing down the Function button and Start/Stop button together (see display at right). Two dots will appear on the display showing that the precondition mode has been accessed; then press Function button to start. Precondition mode runs the centrifuge to ensure full mixing of the chamber air and therefore equilibrates all areas of the rotor and parts for maximum efficiency.

Once this has finished, lift the lid and load samples in position. See page 14 for correct loading procedures.

Note: A slight clicking sound may be heard; this is normal and is just the solenoid gas valve adjusting and allowing for slight variances in temperature.

Display shown when Temp is pressed Display showing a set temperature of 4°C

1. Press Temp numeric box.


2. Set temperature in numeric form (°C).

Note: See rotor information

(on pages 6-11) for minimum possible temperature at max speed per rotor.

Function button

Start button

Function button

Start button

25

Loading and Operating the Centrifuge

Display showing screen when run is finished

(with Buzzer selected)

Display showing running screen

1. Press Lid Open button.

2. Load your samples evenly (see page 14).

3. Fit sealed lid to rotor (if part of rotor type).

4. Close centrifuge lid by pressing both sides down firmly.

5. Press Start button.

6. Centrifuge is now running.

7. If you need to terminate operation early in Time, Hold, or RCFt mode, press Square at bottom right.

8. If you need to terminate operation early in Pulse mode, press Square at bottom right and release when counter (seconds) has reached desired number.

9. Press Run Complete Tick mark

(only shows if Buzzer was selected).

10. Press Lid Open button.

11. You may now retrieve your samples.

26

Maintenance & Care

Cleaning Intervals

For the sake of personal, environmental, and material protection, it is your duty to clean and if necessary disinfect the centrifuge on a regular basis. See recommended cleaning intervals:

Rotor chamber ....... Daily or when polluted

Rotor ....................... Daily or when polluted

Accessories ............ Daily or when polluted

Cabinet ................... Once per month

Ventilation holes ..... Every six months

CAUTION!

Use only approved cleansers. Refrain from using any other cleaning or decontamination procedure than those recommended here. If you are not entirely sure that the intended procedure or cleanser is safe for the equipment, contact the supplier.

Cleaning

Clean centrifuge and accessories as follows:

• Turn off the centrifuge and remove power supply plug.

• Open the centrifuge lid.

• Grab the rotor handle with both hands and lift it vertically from the centrifuge spindle.

• Remove the centrifuge tubes and adapters.

• Use a neutral cleaning agent with a pH value between 6 and 8 for cleaning. Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions, or scrubbing powder.

• Rinse the cavities out thoroughly.

• Use a soft brush without metal bristles to remove stubborn residue.

• Afterwards rinse with distilled water.

• Dry all of the rotors and accessories after cleaning with a soft cloth or in a warm air cabinet at a maximum temperature of 50°C. Higher temperatures could damage the material and shorten the lifetime of the parts.

• After cleaning, treat the entire surface of aluminum parts with corrosion protection oil. Also treat the cavities with oil.

• Treat the bolt of the swing out rotor with bolt grease.

• Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down

CAUTION!

When cleaning, do not allow liquids, especially organic solvents, to get on the drive shaft, the bearings, or the locks. Organic solvents break down the grease in the motor bearing; the drive shaft could lock up.

27

Disinfection

Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation. Decontaminate the affected parts immediately. Take other precautions if need be.

The rotor chamber and the rotor should be treated preferably with a neutral disinfectant. If unsure, contact the supplier for questions regarding the use of other disinfectants.

Observe the safety precautions and handling instructions for the cleaning agents used.

Disinfect the rotor and accessories as follows:



• Grab the rotor handle with both hands and press against the Auto-Lock button. At the same time, pull the rotor directly upwards it from the centrifuge spindle.

• Remove the centrifuge tubes and adapters and dispose of them or disinfect them.

• Treat the rotor and accessories according to the instructions for the disinfectant. Adhere strictly to the given application times.

• Be sure the disinfectant can drain off the rotor.

• Rinse the rotor and rotor li d thoroughly with water and then rub down.

• Dispose of the disinfectant according to the applicable guidelines.

• Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50°C.



WARNING!

Infectious material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions. In case of contamination, make sure that others are not put at risk.

28

Decontamination

Decontaminate the centrifuge immediately whenever radioactive material has spilled during centrifugation. In case of contamination, make sure that others are not put at risk. Decontaminate all affected parts immediately. Take other precautions if need be.

Before using any cleaning or decontamination methods except those recommended by the supplier, users should check with the supplier that the proposed method will not damage the equipment.

For general radioactive decontamination, use a solution of equal parts of 70% ethanol, 10% SDS and water.

Disinfect the rotor and accessories as follows:



• Grab the rotor handle with both hands and press against the Auto-Lock button. At the same time, pull the rotor directly upwards it from the centrifuge spindle.

• Remove the centrifuge tubes and adapters and dispose of them or disinfect them.

• Rinse the rotor first with ethanol and then with deionized water. Adhere strictly to the given application times.

• Be sure the decontamination solution can drain off the rotor. Rinse the rotor and accessories thoroughly with water.

• Dispose of the decontamination solution according to the applicable guidelines.

• Dry all of the rotors and accessories after cleaning with a cloth or in a warm air cabinet at a maximum temperature of 50°C.



WARNING!

Radioactive material can get into the centrifuge when a tube breaks or as a result of spills. Keep in mind the risk of infection when touching the rotor and take all necessary precautions.

Autoclaving

• Clean the rotor before autoclaving and rinse thoroughly with distilled water.

• Remove all accessories (tubes, adapters) from the rotor. Place the rotor on a flat surface.

• Rotors and adapter can be autoclaved at 121°C (250°C) with a maximum permissible autoclave cycle is 20 minutes.

CAUTION!

No chemical additives are permitted in the steam. Never exceed the permitted temperature and duration when autoclaving.

29

Servicing

We recommend having the centrifuge and accessories serviced once a year by an authorized service technician; contact the supplier for details. The service technician checks the following:

• Electrical equipment

• Suitability of the set-up site

• Door lock and safety system

• Rotor

• Installation/fixation of the rotor and the centrifuge spindle

Shipping and Disposing

For proper disposal of the centrifuge, refer to the regulations in your country. If in doubt, contact the supplier. Also refer to "Transporting the Centrifuge" and "Shipping the Centrifuge" on pages 12-13.

For the countries of the European Union the disposal is regulated by the European Union's Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC.

WARNING!

When removing the centrifuge and accessories from use for disposal, you must clean and if necessary disinfect or decontaminate the entire system. If in doubt, contact Cole-Parmer.

Performance Recommendations

• Always keep buckets and adapters clean and free from moisture at all times, this will help prevent corrosion and keep them in good, safe operating condition.

• Use only supplier-approved accessories with your product.

• Always ensure samples are distributed in a diametric pattern to ensure the unit runs balanced.

• Rotor pins should be kept clean and lubricated on a regular basis.

• It is recommended that your centrifuge is switched off and the lid left open overnight to ensure that any moisture build up can evaporate.

• Always ensure that lids and accessories are correctly tightened before running the unit.

• Ensure that all buckets are present in the rotor when running your centrifuge, as using the unit with an unbalanced rotor can cause distortion and excessive wear.

• Do not use any kind of scourers or abrasive material to clean the buckets and ensure that a pH neutral cleaner is always used.

• It is recommended that regular maintenance and cleaning of the unit and accessories is carried out.

• Be sure to make note of the rotor expiration date which can be found on the underside of your rotor.

30

Troubleshooting

Mechanical Emergency Door Release

During a power failure, you will not be able to open the centrifuge lid with the regular electric lid release. A mechanical override is provided to allow sample recovery in the case of an emergency. However, this should be used only in emergencies and after the rotor has come to a complete stop

WARNING!

The rotor can still be spinning at high speed. If touched, it can cause serious injuries. Always wait until the rotor has come to a complete stop without braking. The brake does not work when there is no current. The braking process lasts much longer than usual.

Proceed as follows:

1. Make sure the rotor has stopped (view port in the lid).

WARNING!

Never use your hands or other tools to brake the rotor.

2. Pull out the power supply plug.

3. On the bottom of the housing is one black plastic plug which you can pry out of the side plate with a screwdriver. It is located below the front side of the centrifuge. It is reachable with a screwdriver. Pull the release cord attached to it to trigger the mechanical door release. The door will open and the samples can be removed. Open the front door.

Overhang the unit to gain access to emergency lid release. Mechanical Emergency Door Release on the underside.

4. Place the centrifuge so that you have access to the downside of the front panel.

CAUTION!

Due to its weight, the centrifuge should be carried by several people. Always lift the centrifuge from both sides.

5. Push the cord back into the centrifuge and mount the plug.

6. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge.

7. Press the OPEN key to have the door locks operative again.

31

Error Codes Shown on the Display

Rotor out of Balance.

1. When centrifuge has stopped, press the Tick button.

2. Press the Lid Open button.

3. An imbalance as occurred. Check for improperly loaded tubes (see page 14), broken tubes, or incorrectly measured tube.

Tacho Failure

1. When centrifuge has stopped, press the Tick button.

2. Press the Lid Open button.

3. Check that tubes are not too tall or if anything else is stopping the rotation of the rotor.

Lid Open!

1. Check that lid is closed correctly. Press down firmly on lid at both sides near the front.

2. If that doesn’t work, then check the sensor (see below) or call Cole-Parmer.

How to Check Sensors

Make sure the centrifuge lid is open. Diagnostics

Go to parameters page.

Press “di” (small top-right button) for the Diagnostics screen.

Either 0 or 1 may show when this screen first appears. Then follow instructions below to perform diagnostics checks.

--------------- or greater (version number of installed firmware)

--------------- 0 or 1: by rotating rotor Tacho will show 0 or 1 as rotor turns if working properly.

--------------- 0 or 1: by rotating rotor Rotor Rec will show 0 or 1 as rotor turns if working properly.

--------------- 0 or 1: by pressing rotor firmly or shaking hard, Balance will show 0 or 1.

--------------- 0 or 1: by shutting the lid, the Lid switch will go from 0 to 1 if working properly.

32

Error Messages and Troubleshooting

Rotor out of Balance shown on the display

An imbalance has occurred.

Check the sample tubes for equal and opposite positioning in rotor.

See “Programming & Operation” section for correct loading and instructions.

Turn off the centrifuge at the power inlet and wait 2 minutes. If message still shows on display, check to see if the detector is damaged.

If none of the above, contact Cole-Parmer.

Tacho Failure shown on the display

Note this does not necessarily mean a Tachometer or board problem.

First check that the sample tubes are not too tall and preventing the rotor to move when the lid is closed.

Check the rotor and motor for movement; they must rotate freely and smoothly.

Check the tachometer on the underside for dust or intrusions to the sensor.


Speed will not go beyond 2000 rpm

Electronics have set the rotor into safety mode; unit has automatic rotor recognition.

Check the sensor on top of the motor, check the magnets are clean (wipe with a dry cloth) on underside of rotor.


Noisy

Balance the samples in the rotor, check that the lid seals are in place and in good order.

Lid support is weak

Gas strut failure, contact Cole-Parmer.

For Information, Contact:

Toll-Free: 1-800-323-4340

Phone: 1-847-549-7600

Fax: 1-847-247-2929

ColeParmer.com

Note: If you need to contact Cole-Parmer for troubleshooting or repair, please provide the model number and serial number of your centrifuge.

Date post:	21-Jun-2020
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