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OPTIMIZATION OF CARBON DIOXIDE OPTIMIZATION OF CARBON DIOXIDE
SEQUESTRATION USING A NOVEL HYBRID SEQUESTRATION USING A NOVEL HYBRID
PHOTOBIOREACTOR FOR PHOTOBIOREACTOR FOR
CHLORELLA HOMOSPHAERA CHLORELLA HOMOSPHAERA CULTIVATIONCULTIVATION
SANDA VELEA, PhDNational Research & Development Institute for Chemistry & Petrochemistry –
ICECHIM, Bucharest, ROMANIA
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki, November 1-3, 2012
Materials and methodsMaterials and methods
Kinetic parameters calculation Kinetic parameters calculation
Experimental results Experimental results
ConclusionsConclusions
OverviewOverview
OUTLINEOUTLINE
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki, November 1-3, 2012
Biological carbon sequestration in algal culture could be environmentally friendly and cost-effective means of reducing carbon dioxide emissions in the energy sector.
A novel hybrid photobioreactor, which combines the advantages of an open system with those of a flat plate photobioreactor was developed to improve high surface-to-volume ratio of photobioreactor and the photosynthetic efficiency by enriched CO2-sequestration via bubbling of CO2 into the culture medium to achieve high biomass productivities.
A case study to evaluate the performance of novel photobioreactor was performed to assess biomass productivity and parameters efficiency associated with the conversion of carbon dioxide in the algal photosynthesis process for Chlorella homosphaera culture.
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OVERVIEWOVERVIEW
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki, November 1-3, 2012
Chlorella homosphaera is an original strain deposited in
the Culture Collection of Algae
and Protozoa at the Scottish
Marine Institute, Scotland, as
CCAP 211/121
ALGAE STRAINALGAE STRAIN
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
Components Concentration (g/L)NaHCO3 16,80
K2HPO4 0,50
NaNO3 2,50
K2SO4 1,00
NaCl 1,00
MgSO4 · 7H2O 0,20
CaCl2 · 2H2O 0,04
Solution of microelements 1 mLSolution of chelated Fe2+ 5 mL
Micronutrients - stock solution Concentration (g/L)
H3BO3 2,860
MnSO4 · 4H2O 2,030
ZnSO4 · 7H2O 0,222
MoO3 (85%) 0,018
Cu SO4 · 5H2O 0,079
Co(NO3)2· 6H2O 0,494
CULTURE MEDIUM COMPOSITIONCULTURE MEDIUM COMPOSITION
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
CULTURE CONDITIONSCULTURE CONDITIONS
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
PARAMETERS MEASUREDPARAMETERS MEASURED
The novel hybrid photobioreactor combines the advantages of an open system with those of a flat plate photobioreactor to enhance surface-to-volume ratio of photobioreactor and the photosynthetic efficiency
The novel hybrid photobioreactor combines the advantages of an open system with those of a flat plate photobioreactor to enhance surface-to-volume ratio of photobioreactor and the photosynthetic efficiency
Aerial view of photobioreactorFront view of photobioreactor
Flow schematic of the hybrid photobioreactor system
HYBRID PHOTOBIOREACTORHYBRID PHOTOBIOREACTOR
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
Types Working volume
(L)
Culture depth in OT (mm)
Surface/Volume ratio
(m-1)
Mixing time (s)
CTa 48 100 10 150
HPB-1b 66 100 19.5 360
HPB-2c 42 50 30.7 240
a CT – Control tank is an open tank, working volume of 48 L and culture depth of 100 mm b HPB 1 – Hybrid photobioreactor, , working volume of 66 L and culture depth of 100 mm c HPB 2 – Hybrid photobioreactor, , working volume of 42 L and culture depth of 50 mm
TECHNICAL CARACTERISTICSTECHNICAL CARACTERISTICS
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
No Kinetic parameter Equation Caption
1 Exponential growth rate R - exponential growth rate X - population size
2 Doubling time T – doubling time
3 Nitrogen fixation FN - nitrogen fixation B – biomassN – nitrogenV - volumeM – molecular weight
4 Phosphorus fixation FP – phosphorus fixation
5 Carbon fixation FC – carbon fixation C – carbon
6 Carbon dioxide fixation FD – carbon dioxide fixation
7 Specific consumption of carbon dioxide
CS - Specific consumption
KINETIC PARAMETERS CALCULATION KINETIC PARAMETERS CALCULATION
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
CALIBRATION CURVE OF BIOMASS CALIBRATION CURVE OF BIOMASS CONCENTRATIONCONCENTRATION
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
OD – optical density - absorbance at 678 nm B - biomass FD - carbon dioxide fixation FN - nitrogen fixation FP – phosphorus fixation
EXPONENTIAL GROWTH RATE CALCULATIONEXPONENTIAL GROWTH RATE CALCULATION
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
TIME COURSES OF BIOMASS CONCENTRATIONTIME COURSES OF BIOMASS CONCENTRATION
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
EXPONENTIAL GROWTH RATEEXPONENTIAL GROWTH RATEand DOUBLING TIMEand DOUBLING TIME
as function of different parametersas function of different parameters
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
DAILY CARBON DIOXIDE FIXATIONDAILY CARBON DIOXIDE FIXATIONand SPECIFIC CONSUMPTION OF COand SPECIFIC CONSUMPTION OF CO22
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
Type Biomass concentratio
n (g/L)
Volumetric productivity
(g/L day)
Total biomass
(g)
Exponential growth rate
(day-1)
Doubling time (day)
Maximum CO2
fixation (%)
CTa 1,2 ± 0.2 0.150 ± 0.025 57.6 ± 2.6 0.37 ± 0.02 1.85 ±0.05 27 ± 2
HPB-1b 2.5 ± 0.2 0.312 ± 0.025 165.0 ±
5.2
0.63 ± 0.02 1.09 ± 0.05 55 ± 2
HPB-2c 3.6 ± 0.2 0.450 ± 0.025 151.2 ±
4.4
0.87 ± 0.02 0.79 ± 0.05 65 ± 2
PERFORMANCES OF NOVEL HYBRID PERFORMANCES OF NOVEL HYBRID PHOTOBIOREACTORPHOTOBIOREACTOR
a CT – Control tank is an open tank, working volume of 48 L and culture depth of 100 mm b HPB 1 – Hybrid photobioreactor, , working volume of 66 L and culture depth of 100 mm c HPB 2 – Hybrid photobioreactor, , working volume of 42 L and culture depth of 50 mm
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
CONCLUSIONSCONCLUSIONS
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
The novel hybrid photobioreactor is a very promising microalgae production system, in which the combination of an open system with a flat plate photobioreactor enhances the flow dynamics and gas exchange in the water–air interface, due to 30 fold higher surface to volume ratio compared to standard open tank, and thereby produces increased biomass, dense algal cultures and a very good sequestration of carbon dioxide,.
C. homosphaera produced a maximum biomass yield of 3.6 g/L in HPB-2, which is 3 fold increased compared to a standard open tank system, on the 11th day.
The runs in HPB-2 showed higher values for exponential growth rates (R=0.87 day-1) than standard open tank (R=0.37 day-1) for the same cultures grown in the same media and under the same culture conditions.
System configuration efficiency was quantified by maximum percentage daily carbon dioxide fixation which was 65 ± 2% in HPB-2 that means 2.5 fold increased compared to standard open tank, and by specific consumption of carbon dioxide which was calculated at 1.72 kg per 1 kg of dry algal biomass.
COST Action CM 0903 – UBIOCHEM 3rd Workshop, Thessaloniki:, November 1-3, 2012
Sanda VELEAgeneral.manager@ icechim.ro