OPTOFLUIDIC DEVICES FOR SINGLE CELL MANIPULATION

Ana Rita Ribeiro, Ariel Guerreiro, Pedro Jorge

New Challenges in the European Area - Young

Scientist's 1st International Baku Forum

23-05-2013

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Outline

• Introduction

• Main Principles

• Computational Model and Results

• Experimental Preliminary Results

• Conclusions and Future Work

23-05-2013 Young Scientist's 1st International Baku Forum

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What are optical tweezers?

23-05-2013 Young Scientist's 1st International Baku Forum

Motivation• Manipulation: Cell/particle manipulation withouth

physical contact;

• Miniaturization: Diagnostic devices (in vivo cells, ...)

Main Goals• Development of fiber optical tweezers;

• Study of the forces:

• Numerical models;

• Design and optimization of fiber structures;

• Fabrication of devices;

• Field of application: Manipulation of Red Blood Cells.

Liu, Z. et al Opt. Express, OSA, 2006, 14, 12510-12516

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Main Principles

23-05-2013 Young Scientist's 1st International Baku Forum

Ashkin, A., Dziedzic, J. M., Bjorkholm, J.E. and Chu, S. (1986), Opt Lett., 11, 288-290;Ashkin, A. (1970), Phys. Rev. Lett., 24, 156-159.

Particle placed in the focusing region of a laser beam:

• Force transference of momentum on the particle surface (reflections/refractions).

• Scatering Force

• Gradient Force

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Computational model and Results

23-05-2013 Young Scientist's 1st International Baku Forum

Lorentz Force (dipoles):

𝑓 𝑖=14𝜀0(𝜀𝑝−𝜀𝑚)𝛻𝑖|𝐸|2

R. S. RODRIGUES RIBEIRO et al, “Computational Models for New Fiber Optic Tweezers”, Photonic Sensors

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Results

23-05-2013 Young Scientist's 1st International Baku Forum

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Preliminary Results

• Lens – polimer:

– radius ~ 30-40 μm;

• Laser Diode 980 nm, P = 100mW;

• Polystyrene particles:

23-05-2013 Young Scientist's 1st International Baku Forum

Experimental Preliminary Results

R. S. Rodrigues Ribeiro, et al., New theoretical and experimental methods for the design of fiber optic tweezers, EWOFS, Poland,2013

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Conclusion and Future Work

• Development of a 2D numerical model to calculate optical forces acting on a particle/cell;

• Fabrication of lenses in optical fibers;

• Implementation of a 3D numerical model;

• Study of distinct fiber geometries;

• Manipulation of RBC.

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Thanks!23-05-2013 Young Scientist's 1st International Baku Forum