OVERVIEW

R&D, Laboratories and Analysis Services

Gastien Godin Jacques Gagnon, PhDGeneral Manager Scientific Director

Fishery and Marine Coproducts Division

Fredericton , NBOctober 8th, 2014

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COASTAL ZONES RESEARCH INSTITUTE (CZRI)

• LAND & PEATLANDS MANAGEMENT- AGRICULTURE & RENEWABLE RESOURCES- ADAPTATION TO CLIMATIC CHANGE

• AQUACULTURE• FISHERY AND MARINE COPRODUCTS• LABORATORIES AND ANALYSIS SERVICES

• CZRI Highlights – Value-Added Food Sector

• Mobile Pilot Unit

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Fields of Expertise and Strategic Research Concentration

LAND & PEATLANDS MANAGEMENT- AGRICULTURE & RENEWABLE RESOURCES- ADAPTATION TO CLIMATIC CHANGE

Dr. Mathieu Quenum – Scientific DirectorEmail : mathieu.quenum@irzc.umcs.ca Tel. : (506) 336-6600

Biomass & Bioenergy Horticulture, Agriculture & Berries Sustainable Production of Sphagnum & Added Value Products Soil, Water & Environment Sustainable Development in Coastal Areas

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Fields of Expertise

AQUACULTUREDr. André Dumas– Scientific DirectorEmail : andré.dumas@irzc.umcs.ca Tel. : (506) 336-6600

Research focus areas

Nutrition of fish and crustaceans Nutrition modelling Arctic charr, salmon, oyster and

lobster production Product development and

innovation management

Nutritional evaluation of commodity and innovative ingredients, prebiotics, probiotics, immunostimulants

Nutrigenomics

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Fields of Expertise

FISHERY AND MARINE COPRODUCTS Dr. Jacques Gagnon– Scientific DirectorEmail : jacques.gagnon@irzc.umcs.ca Tel. : (506) 336-6600

Research focus areas

Development of green processes Lab scale spaces with hydrolysis, drying & centrifugation capacities 53’ pilot multifunctional unit for turnkey project on site Product development and innovation management

Valorization and commercialisation

of marine coproducts Natural health products

LABORATORIES AND ANALYSIS SERVICES

Chemical/microbiological analyses Environmental, nutritional, etc. Food safety ISO/IEC 17025 (CALA)

R&D support Fatty acids & pigments Amino acids (in development)

Personnalized services On-site, technical, process design, etc. Regulatory support, nutritional labelling, shelf life

Coproducts recovery and characterization

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Mobile Pilot Unit

OPEN FOR BUSINESSWe look forward to new collaboration

opportunities!

• 53 feet reefer trailer• Processing room with temp. control• Walk-in cold room and freezer• 600, 220, 110 volts connections

• Various tanks (2 x 1500 liters)• 8 x 100 liters double walls for

temperature control• Various pumps • Filtration unit (sweco)• Mixers• Etc.

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Highlights – Value-Added Food Sector

• CZRI co-hosted (with AFBPA) the Sixth Acadian Peninsula Food Processing Conference

“Growing Our Industry: Making history with the new biotechnologies”

Acadian Peninsula Convention Centre, Shippagan, NB May 21-23, 2014

www.shippagan.ca

MAXIMIZING THE VALUE OF MARINE COPRODUCTS:

APPLICATIONS IN NUTRISCIENCES

Jacques Gagnon, PhD and colls.

Fisheries Council of Canada 2014 ConferenceOctober 8th

Valorization of effluents, residues and marine coproducts

VALEUR.COM VALEUR.COM = VALorisation

des Effluents d'Usine, des Résidus et des COproduits Marins)

Full title –AIF project:DEVELOPMENT AND COMMERCIALIZATION OF MARINE COPRODUCTS FOR USE IN ANIMAL AND HUMAN NUTRITION AND ITS APPLICATION TO THE PREVENTION OF OBESITY, DIABETES AND NEURODEGENERESCENCE CZRI research team: Nadia Tchoukanova

Sébastien Plante and Jacques Gagnon

BioAtlantech Survey (Biosciences)Geographical

clustering aquaculture and marineSt. George/St. Andrews

(7 Companies + 2 Research Institutions)

Acadian Peninsula (7 Companies + 1 Research Institution)

Now BioNB, 2013

LIST OF COPRODUCTS

SEA, PROCESSING AND AQUAFEED: A

VALUE CREATION PATHWAY OF

SEAFOOD RAW MATERIALS

André Dumas, PhD & colls

The Fisheries Council of Canada 2014 conference

Aquaculture in the World

Source: FAO (2012)

020406080

100

2000 2010 2020

MT

(mill

ions

)

Aquafeeds in the World

• 20 to 23 millions MT aquafeed/yr (2006)

• ~3.7 million MT/yr fish meal (FM) = $5.5 bln

• ~0,8 million MT/yr of fish oil (FO) = $1.0 bln

Source: Tacon & Metian (2008)

FM: source of beneficial nutrients

• Essential amino acids (AA)• Valuable AA: Taurine, hydroxyproline • Vitamins (e.g. B12)• Minerals (e.g. available P)

FO: source of beneficial fatty acids

• Omega-3 to meet consumer expectations

FM & FO cost

Source: Tacon & Metian (2008)

• Seafood processing by-products from sustainably managed fisheries

• New business opportunities

Alternatives to FM & FO: what does the sea have to offer?

Shrimp processing

Residues (70%) Liquid

SolidsPhotos: http://dailyfoodporn.wordpress.com/tag/nordic-shrimp/

Recycled

30%

Shrimp residue meal

Shrimp residue meal

0

10

20

30

40

50

60

70

Protein Lipid Ash Fiber P

% o

f DM

Proximate composition

Shrimp residue meal

0,0

1,0

2,0

3,0

4,0

5,0

6,0

7,0

Arg Cys His Iso Leu Lys Met Phe Thr Try Val

% o

f DM

EAA

Shrimp residue

Herring

Shrimp residue meal (SRM)

0

2

4

6

Initial Final

Body

wei

ght (

g)

Commercial SRM

CONCLUSION

1. SRM:

•Candidate alternative to $$$ sources of

beneficial nutrients

2. Next steps

•Approval by regulatory agencies

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LABORATORIES AND ANALYSIS SERVICES:

BIOCHEMICAL CHARACTERIZATION OF MARINE PRODUCTS AND COPRODUCTS

Josée Boudreau, PhD, Marie-Hélène Thibault, PhD, Claude Pelletier, M.Sc, Mathieu Ferron, M.SC

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MARINE PROCESSING COPRODUCTS

Over the years, our multidisciplinary team has developped an expertise in the screening and characterization of several marine processing coproducts such as:

HerringArctic shrimp

Lobster Snow crabSea cucumber

• Mouth• Anus• Viscera

• Head • Hepatopancreas• Shell

• Cephalothorax• Shell• Protein waste

• Head• Tail• Viscera

• Milt

• Roe

Crude pro-tein23%

Crude fat5%

Ash1%

Humidity70%

Crude protein

19%

Crude fat3%

Ash2%

Humidity77%

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CHARACTERIZATION OF MARINE COPRODUCTS

In a first step, proximate analysis, using standard methods, reveals the major constituents in the products under study

Immature herring roe Herring milt

Crude protein

23%Crude

fat5%Ash

1%

Humidity70%

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CHARACTERIZATION OF MARINE COPRODUCTS

Each class of compounds can be probed further to gain knowledge about the product at hand and the biomolecules it contains

Immature herring roe

• Marine products are known to be rich in phospholipids and omega-3 fatty acids, as well as fat-soluble biomolecules such as vitamins (A, D, E) and pigments

• Lipids are therefore often one of the main focuses in the study of marine coproducts

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MARINE COPRODUCT LIPIDS

Each Lipid classes Fatty acid profile

Rancidity/oxidation Other bioactive molecules

• Phospholipids• Sterols• Triglycerides• Wax esters

• Neutral vs polar lipids

• Omega 3• Omega 6• EPA, DHA

• Saturated

• Mono-, poly-unsaturated

• Free fatty acids (FFA)• Peroxide value• p-anisidine value

• Vitamins (A, D and E)• Carotenoid pigments

(Astaxanthin, β-carotene, etc.)

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MARINE COPRODUCT PIGMENTS

Current projects in our laboratories include the detailed study and characterization of astaxanthin, a widespread and commercially relevant marine pigment, in shellfish processing coproducts.

For animals:• Widely used in aquaculture for flesh

pigmentation improved fish growth

For humans:• Powerful antioxidant • Anti-tumor • Anti-inflammatory • Protects against cardiovascular

diseases and ulcers• Treats neurodegenerative diseases

Astaxanthin

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ASTAXANTHIN ANALYSIS

Various chemical forms of astaxanthin naturally occur and influence its biological properties and stability. Different analytical methods are required to study these complex molecules.

Astaxanthin

2. Geometrical isomersAll trans, 9-cis, 13-cis, etc.

1. Free or with fatty acid(s)Free, monoester or diester

3. Optical isomers{R,R’}, {R,S’} (MESO), {S,S’}

Current analytical capabilities in our laboratories include:

Quantification of free, esterified and total astaxanthin Identification of geometrical isomers (all trans, 9-cis, 13-cis, etc.)

Work is in progress to develop a rapid, simple, in situ method for the identification of astaxanthin optical isomers in different products

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ASTAXANTHIN ANALYSIS

High performance liquid chromatography (HPLC)

Normal phase Reversed phase

Chiral reversed phase

RAMAN and FT-Infrared microspectroscopy

Microscope for in situ imaging « Same spot » technology for

heterogeneous sample mapping

Fishery coproduct as nutraceutics (natural health products and functional foods):

Innovation based technology

Diabetes

Obesity

Inflammation

Neurodegeneration

Insulin resistance

1. Anti-diabetes

2. Anti-inflammatory

3. Anti-obesity

4. Neuroprotection

Valorization of Coproducts from fish processing plants

CZRIPurification AnalysesProcess developmentPilot scale unit

NRC- HalifaxBioguided fractionationPurificationAdipocytes cell model

(Obesity)

NRC- Charlottetown in vitro and in vivo

diabetes modelBioguided fractionation

Dalhousie University in vitro and in vivo

Alzheimer model

Jacques Gagnon

Dr. Bob Chapman

• Product formulation• Product stability• Market opportunities

• Animal feed• Natural health

products (NHPs)• Human food

products

CZRI

Lead

bioactives

New, added-value

products

• In vitro bioassays• In vivo models

• Natural product chemistry extraction

• Chemical fractionation• Chemical

characterization

• Fish cuttings• Shrimp• Sea cucumbers

• Scale up• Food technology centers• Partnering initiatives

CZRI phases

Product

development

Chemical

analysis

Bio-analysis

Aquaculture or marinebiomass

Summary

• Extracts and protein hydrolysates prepared: ~100

• Focus on lead products that would be beneficial in metabolics disorders (e.g. pre-diabetes, obesity), be neuroprotective, or anti-inflammatory

• Shrimp extracts and herring milt hydrolysates gave good activity for antidiabetic and neuroprotective effects

• These should be further assessed by the development of health supplement ingredients with animal model of alzheimer’s disease and diabetes

Jacques Gagnon

Funding Agencies• Atlantic Canada Opportunities Agencies

(ACOA)- Atlantic Innovation Fund (AIF)

• New Brunswick Innovation Fund (NBIF)

• Industrial Partners: ex: Island Fishermen Cooperative Association

• New Brunswick Department of Agriculture, Aquaculture and Fisheries

Jacques Gagnon

Patent No. Kind Date Application No. DateWO 2014138920 A1 Sep 18, 2014 WO 2014-CA251 Mar 13, 2014

What is in the pipelinePatent published… more to come with

antidiabetic activities of shrimp oilMarket feasability study with shrimp oil in

progressAnimal model of diabetes and

neurodegeneration with shrimp oil and herring milt hydrolysate to confirm positive effects are in progress

Looking for a commercial receptor partnerData collection in animal models to support a

human clinical trial with shrimp oil (NHP)Purification process of shrimp oil for human

use at lab scale- with scaling up at industrial level

Jacques Gagnon

Questions?

THANK YOU